Reviewed, UniProtKB/Swiss-Prot P32440 (HMD_METTM)

Names and origin

Protein names

Recommended name:
    5,10-methenyltetrahydromethanopterin hydrogenase
    EC=1.12.98.2
Alternative name(s):
    H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase
    N(5),N(10)-methenyltetrahydromethanopterin hydrogenase
    H(2)-dependent methylene-H(4)MPT dehydrogenase

Gene names

Name:	hmd
Synonyms:	mth

Organism

Methanobacterium thermoautotrophicum (strain Marburg / DSM 2133)

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible reduction of methenyl-H4MPT+ to methylene-H4MPT. HAMAP MF_01090
Catalytic activity	H2 + 5,10-methenyltetrahydromethanopterin = H+ + 5,10-methylenetetrahydromethanopterin. HAMAP MF_01090
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methylene-H(4)MPT from 5,10-methenyl-H(4)MPT (hydrogen route): step 1/1. HAMAP MF_01090
Sequence similarities	Belongs to the HMD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	methanogenesis, from carbon dioxide Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N5,N10-methenyltetrahydromethanopterin hydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	5,10-methenyltetrahydromethanopterin hydrogenase HAMAP MF_01090		PRO_0000218512
Experimental info
	Sequence conflict	74 – 75	2	DD → EH in CAA42121. Ref.1
	Sequence conflict	304 – 305	2	ID → RY in CAA42121. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32440-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 5DA41A4BCA5ED64A	FASTA	344	37,704
	10 20 30 40 50 60 MKLAILGAGC YRTHAASGIT NFSRACEVAE MVGKPEIAMT HSTITMGAEL KELAGVDEVV 70 80 90 100 110 120 VADPVFDNQF TVIDDFAYED VIEAHKEDPE NIMPQIREKV NEVAKELPKP PEGAIHFTHP 130 140 150 160 170 180 EDLGFEITTD DREAVADADF IMTWFPKGDM QPDIINKFID DIKPGAIVTH ACTIPTTKFY 190 200 210 220 230 240 KIFEQKHGDL VTKPETLNVT SYHPGAVPEM KGQVYIAEGY ASEDAIETLF ELGQKARGNA 250 260 270 280 290 300 YRLPAELLGP VCDMCSALTA MTYACILSYR DSVTQVLGAP ASFAQMMAKE SLEQITALME 310 320 330 340 KVGIDKMEEN LDPGALLGTA DSMNFGASAE ILPTVFEILE KRKK

References

[1]	"Hydrogen-forming and coenzyme-F420-reducing methylene tetrahydromethanopterin dehydrogenase are genetically distinct enzymes in Methanobacterium thermoautotrophicum (Marburg)." Von Buenau R., Zirngibl C., Thauer R.K., Klein A. Eur. J. Biochem. 202:1205-1208(1991) [PubMed: 1765081] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"H2-forming methylenetetrahydromethanopterin dehydrogenase, a novel type of hydrogenase without iron-sulfur clusters in methanogenic archaea." Zirngibl C., van Dongen W., Schwoerer B., von Buenau R., Richter M., Klein A., Thauer R.K. Eur. J. Biochem. 208:511-520(1992) [PubMed: 1521540] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59547 Genomic DNA. Translation: CAA42121.1.
PIR	S19705.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_01090. [Tree]
InterPro	IPR004889. HMD. IPR010062. HMD_sub. [Graphical view]
Pfam	PF03201. HMD. 1 hit. [Graphical view]
TIGRFAMs	TIGR01723. hmd_TIGR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HMD_METTM

Accession

Primary (citable) accession number: P32440

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 5, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)