Reviewed, UniProtKB/Swiss-Prot P32434 (PGTB1_SCHPO)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl transferase type-1 subunit beta
    EC=2.5.1.59
Alternative name(s):
    Geranylgeranyl transferase type I subunit beta
      Short name=GGTase-I-beta
    Type I protein geranyl-geranyltransferase subunit beta
      Short name=PGGT

Gene names

Name:	cwg2
ORF Names:	SPAC2E1P5.04c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal C-A-A-L where A is an aliphatic amino acid. In particular it modifies the GTP-binding component of the 1,3-beta-D-glucan synthase.
Catalytic activity	Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Heterodimer of an alpha and a beta subunit.
Sequence similarities	Belongs to the protein prenyltransferase subunit beta family. Contains 4 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Prenyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	1,3-beta-glucan biosynthetic process Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe establishment or maintenance of cell polarity Inferred from mutant phenotype. Source: GeneDB_SPombe protein amino acid geranylgeranylation Inferred by curator. Source: GeneDB_SPombe
Cellular component	CAAX-protein geranylgeranyltransferase complex Non-traceable author statement. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	CAAX-protein geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC Rab geranylgeranyltransferase activity Inferred from genetic interaction. Source: GeneDB_SPombe protein binding Ref.1 Inferred from physical interaction. Source: GeneDB_SPombe zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Geranylgeranyl transferase type-1 subunit beta		PRO_0000119778
Regions
	Repeat	119 – 163	45	PFTB 1
	Repeat	169 – 210	42	PFTB 2
	Repeat	234 – 275	42	PFTB 3
	Repeat	282 – 324	43	PFTB 4
Sites
	Metal binding	260	1	Zinc By similarity
	Metal binding	262	1	Zinc By similarity
	Metal binding	312	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32434-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 811A3CBFF0067071	FASTA	355	40,023
	10 20 30 40 50 60 MELTRAKHIA FFKRHLILFP TPYEEHDCER TVLAFFCLLG LDLLNALNTI DDDDKKSWIE 70 80 90 100 110 120 WIYKNYVTKE SKGIKYSGFQ AYRTGIQPIS FEQEPQLAGT VFSICCLLFL GDNLSRIDRD 130 140 150 160 170 180 LIKNFVELCK TSQGHFRSIA VPSCSDQDMR QLYMATTIAS LLDFSLSDPL CSIQYIKSCQ 190 200 210 220 230 240 RYEGGFSLLP YGEAHAGATF CALASWSLIL KMIPNSSLNT SNQSYNLMDC VPKVERLIRW 250 260 270 280 290 300 LASRQLSSGG LNGRTNKDVD TCYAYWVLSS LKLLDALPFI DGGELEKYLL LHAQHALGGF 310 320 330 340 350 SKTPGEFPDV LHSALGLYAM AYQDDKSFPK VNADIHMTSK YINICRDCIQ AAKGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Schizosaccharomyces pombe cwg2+ gene codes for the beta subunit of a geranylgeranyltransferase type I required for beta-glucan synthesis." Diaz M., Sanchez Y., Bennett T., Dun C.R., Godoy C., Tamanoi F., Duran A., Perez P. EMBO J. 12:5245-5254(1993) [PubMed: 8262067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z12155 Genomic DNA. Translation: CAA78143.1. CU329670 Genomic DNA. Translation: CAB86347.1.
PIR	S41686.
RefSeq	NP_594142.1.
3D structure databases
HSSP	HSSP built from PDB template 1D8D based on UniProtKB Q02293.
ModBase	Search...
Protein-protein interaction databases
STRING	P32434.
Genome annotation databases
GeneID	2541514.
GenomeReviews	Gene locus cwg2 in contig CU329670_GR.
KEGG	spo:SPAC2E1P5.04c.
NMPDR	fig|4896.1.peg.4112.
Organism-specific databases
GeneDB_Spombe	SPAC2E1P5.04c.
Phylogenomic databases
OMA	CYIAVAL.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001764-MON.
BRENDA	2.5.1.59. 653.
Gene expression databases
ArrayExpress	P32434.
Family and domain databases
InterPro	IPR001330. Prenyltrans. [Graphical view]
Pfam	PF00432. Prenyltrans. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGTB1_SCHPO

Accession

Primary (citable) accession number: P32434

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 3, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)