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P32432

- SFP1_YEAST

UniProt

P32432 - SFP1_YEAST

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Protein

Transcription factor SFP1

Gene

SFP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor that regulates ribosomal protein (RP) and ribosome biogenesis (Ribi) gene expression in response to nutrients and stress. Promotes RP gene expression under optimal growth conditions. Leaves the nucleus upon environmental challenges, resulting in a down-regulation of RP gene transcription. The effect of the environmental cues on SFP1 localization is mediated through the TOR pathway. Also regulates the expression of genes involved in the G2/M transition during the mitotic cell cycle and the DNA-damage response. Required for carbon-source modulation of cell size.5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri598 – 62326C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri659 – 68325C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter Source: SGD
  2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  3. regulation of cell size Source: SGD
  4. response to stress Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Prion

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32465-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SFP1
Alternative name(s):
Split finger protein 1
Gene namesi
Name:SFP1
Ordered Locus Names:YLR403W
ORF Names:L8084.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR403w.
SGDiS000004395. SFP1.

Subcellular locationi

Cytoplasm. Nucleus
Note: Nuclear under optimal growth conditions. Leaves the nucleus in response to stress or changes in nutrient availability. The [ISP+] aggregates appear to be nuclear.

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Amyloid, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 683683Transcription factor SFP1PRO_0000046851Add
BLAST

Post-translational modificationi

Phosphorylated by TORC1 kinase at multiple sites. Phosphorylation regulates nuclear localization and RP promoter binding.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32432.
PaxDbiP32432.
PeptideAtlasiP32432.

Expressioni

Gene expression databases

GenevestigatoriP32432.

Interactioni

Subunit structurei

Interacts with the target of rapamycin complex 1 (TORC1) in a rapamycin-dependent manner. Interacts with MRS6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MRS6P328644EBI-17035,EBI-14799

Protein-protein interaction databases

BioGridi31660. 50 interactions.
DIPiDIP-1379N.
IntActiP32432. 11 interactions.
MINTiMINT-410747.
STRINGi4932.YLR403W.

Structurei

3D structure databases

ProteinModelPortaliP32432.
SMRiP32432. Positions 592-619, 652-677.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni230 – 458229Prion domain (PrD)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi532 – 55524Poly-AspAdd
BLAST

Domaini

The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.By similarity

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri598 – 62326C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri659 – 68325C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5189.
GeneTreeiENSGT00390000003635.
InParanoidiP32432.
OMAiNQKLHEN.
OrthoDBiEOG7P5TBB.

Family and domain databases

Gene3Di3.30.160.60. 1 hit.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32432-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDFTTMTMAS NMATSTTTTA TSAHASINSS SNFNIDIDSN QNTPSILINN
60 70 80 90 100
NSDSSNGKNT DFNGVNNIHQ KNIMNNTNNV HLYSPNIMDQ TLLTPQDIAK
110 120 130 140 150
LRRESIAHSQ GMGGVSWGSI SVGSWLRDEI ISRRNSIVPA SANGAASAAA
160 170 180 190 200
SATTTATNTL QIQQPTKRPS VSNPPYHRGY SISPQIAYTA YLPNLEKQYC
210 220 230 240 250
KDYSCCGLSL PGLHDLLRHY EEAHISTSPN TTNMSQIPMN SAGNTSSSVR
260 270 280 290 300
MTNNTSSANY NLQNNMAANT KNAGHKTNTM QAHSSNATNN TSINNMHANL
310 320 330 340 350
QSNMDSNSTI RQSQHPHHQQ NIIQQQLQSN SVNHTSGAVP TPSVMGSATA
360 370 380 390 400
SSTTANPNVI SITGAPNSGL SMANHSQQLH LNGNLVDAVS TNDVFLRTSN
410 420 430 440 450
SPSRHVPHNK QINSNNNSGI NINNNTSHNS NINMGSKNAM VNRPHTFNNY
460 470 480 490 500
SLNKTSRNPI QHQSRKIDPH QTDLSPLVLV QDIDLSFMDD DILGPSNHNS
510 520 530 540 550
MNSVVNPTTG SHNYNTFHSS VHAKSSQNMV EDQDIDDIDD DDDVDDDDDD
560 570 580 590 600
DDDDDTENGS SSNGKSVHNN NYKMPQQAYI DDPARRLYVM DHEEQKPFKC
610 620 630 640 650
PVIGCEKTYK NQNGLKYHRL HGHQNQKLHE NPDGTFSVID PDSTDSFGDG
660 670 680
MGSAKDKPYR CEVCGKRYKN LNGLKYHRGH STH
Length:683
Mass (Da):74,785
Last modified:October 1, 1993 - v1
Checksum:i0332A381D75A6F14
GO

Sequence cautioni

The sequence AAA35041.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63577 Genomic DNA. Translation: AAA35041.1. Different initiation.
U19729 Genomic DNA. Translation: AAB82343.1.
BK006945 Genomic DNA. Translation: DAA09702.1.
PIRiJH0497.
RefSeqiNP_013507.1. NM_001182291.1.

Genome annotation databases

EnsemblFungiiYLR403W; YLR403W; YLR403W.
GeneIDi851119.
KEGGisce:YLR403W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63577 Genomic DNA. Translation: AAA35041.1 . Different initiation.
U19729 Genomic DNA. Translation: AAB82343.1 .
BK006945 Genomic DNA. Translation: DAA09702.1 .
PIRi JH0497.
RefSeqi NP_013507.1. NM_001182291.1.

3D structure databases

ProteinModelPortali P32432.
SMRi P32432. Positions 592-619, 652-677.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31660. 50 interactions.
DIPi DIP-1379N.
IntActi P32432. 11 interactions.
MINTi MINT-410747.
STRINGi 4932.YLR403W.

Proteomic databases

MaxQBi P32432.
PaxDbi P32432.
PeptideAtlasi P32432.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR403W ; YLR403W ; YLR403W .
GeneIDi 851119.
KEGGi sce:YLR403W.

Organism-specific databases

CYGDi YLR403w.
SGDi S000004395. SFP1.

Phylogenomic databases

eggNOGi COG5189.
GeneTreei ENSGT00390000003635.
InParanoidi P32432.
OMAi NQKLHEN.
OrthoDBi EOG7P5TBB.

Enzyme and pathway databases

BioCyci YEAST:G3O-32465-MONOMER.

Miscellaneous databases

NextBioi 967839.
PROi P32432.

Gene expression databases

Genevestigatori P32432.

Family and domain databases

Gene3Di 3.30.160.60. 1 hit.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 3 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A split zinc-finger protein is required for normal yeast growth."
    Blumberg H., Silver P.
    Gene 107:101-110(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The SFP1 gene product of Saccharomyces cerevisiae regulates G2/M transitions during the mitotic cell cycle and DNA-damage response."
    Xu Z., Norris D.
    Genetics 150:1419-1428(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Novel non-Mendelian determinant involved in the control of translation accuracy in Saccharomyces cerevisiae."
    Volkov K.V., Aksenova A.Y., Soom M.J., Osipov K.V., Svitin A.V., Kurischko C., Shkundina I.S., Ter-Avanesyan M.D., Inge-Vechtomov S.G., Mironova L.N.
    Genetics 160:25-36(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PRION FORM.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A dynamic transcriptional network communicates growth potential to ribosome synthesis and critical cell size."
    Jorgensen P., Rupes I., Sharom J.R., Schneper L., Broach J.R., Tyers M.
    Genes Dev. 18:2491-2505(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Sfp1 is a stress- and nutrient-sensitive regulator of ribosomal protein gene expression."
    Marion R.M., Regev A., Segal E., Barash Y., Koller D., Friedman N., O'Shea E.K.
    Proc. Natl. Acad. Sci. U.S.A. 101:14315-14322(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "SFP1 is involved in cell size modulation in respiro-fermentative growth conditions."
    Cipollina C., Alberghina L., Porro D., Vai M.
    Yeast 22:385-399(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Sfp1 interaction with TORC1 and Mrs6 reveals feedback regulation on TOR signaling."
    Lempiainen H., Uotila A., Urban J., Dohnal I., Ammerer G., Loewith R., Shore D.
    Mol. Cell 33:704-716(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TORC1 AND MRS6, PHOSPHORYLATION BY TORC1.
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Non-Mendelian determinant [ISP+] in yeast is a nuclear-residing prion form of the global transcriptional regulator Sfp1."
    Rogoza T., Goginashvili A., Rodionova S., Ivanov M., Viktorovskaya O., Rubel A., Volkov K., Mironova L.
    Proc. Natl. Acad. Sci. U.S.A. 107:10573-10577(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRION FORMATION.

Entry informationi

Entry nameiSFP1_YEAST
AccessioniPrimary (citable) accession number: P32432
Secondary accession number(s): D6VZ36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

[ISP+] is the prion form of SFP1. [ISP+] is the result of a conformational change of the cellular SFP1 protein that becomes self-propagating and infectious. This conformational change generates a form of SFP1 that assembles into amyloid fibrils. [ISP+] aggregates accumulate in the nucleus, and results in significantly larger cell size and increased drug resistance (PubMed:20498075). [ISP+] can be cured by GdnHCl (PubMed:11805042). It is speculated that prion properties of transcription factors may generate an optimized phenotypic heterogeneity that buffers yeast populations against diverse environmental insults (PubMed:20498075).2 Publications
Present with 259 molecules/cell in log phase SD medium.1 Publication

Caution

It is uncertain whether Met-1 or Met-6 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3