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Protein

Transcription factor SFP1

Gene

SFP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that regulates ribosomal protein (RP) and ribosome biogenesis (Ribi) gene expression in response to nutrients and stress. Promotes RP gene expression under optimal growth conditions. Leaves the nucleus upon environmental challenges, resulting in a down-regulation of RP gene transcription. The effect of the environmental cues on SFP1 localization is mediated through the TOR pathway. Also regulates the expression of genes involved in the G2/M transition during the mitotic cell cycle and the DNA-damage response. Required for carbon-source modulation of cell size.5 Publications

Miscellaneous

[ISP+] is the prion form of SFP1. [ISP+] is the result of a conformational change of the cellular SFP1 protein that becomes self-propagating and infectious. This conformational change generates a form of SFP1 that assembles into amyloid fibrils. [ISP+]-aggregates accumulate in the nucleus, and results in significantly larger cell size and increased drug resistance (PubMed:20498075). [ISP+] can be cured by GdnHCl (PubMed:11805042). It is speculated that prion properties of transcription factors may generate an optimized phenotypic heterogeneity that buffers yeast populations against diverse environmental insults (PubMed:20498075).2 Publications
Present with 259 molecules/cell in log phase SD medium.1 Publication

Caution

It is uncertain whether Met-1 or Met-6 is the initiator.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri598 – 623C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri659 – 683C2H2-type 2PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • positive regulation of ribosomal protein gene transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • regulation of cell size Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Prion
Biological processStress response, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32465-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SFP1
Alternative name(s):
Split finger protein 1
Gene namesi
Name:SFP1
Ordered Locus Names:YLR403W
ORF Names:L8084.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR403W
SGDiS000004395 SFP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Amyloid, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000468511 – 683Transcription factor SFP1Add BLAST683

Post-translational modificationi

Phosphorylated by TORC1 kinase at multiple sites. Phosphorylation regulates nuclear localization and RP promoter binding.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32432
PaxDbiP32432
PRIDEiP32432

PTM databases

iPTMnetiP32432

Interactioni

Subunit structurei

Interacts with the target of rapamycin complex 1 (TORC1) in a rapamycin-dependent manner. Interacts with MRS6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MRS6P328645EBI-17035,EBI-14799

Protein-protein interaction databases

BioGridi31660, 53 interactors
DIPiDIP-1379N
IntActiP32432, 19 interactors
MINTiP32432
STRINGi4932.YLR403W

Structurei

3D structure databases

ProteinModelPortaliP32432
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni230 – 458Prion domain (PrD)Add BLAST229

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi532 – 555Poly-AspAdd BLAST24

Domaini

The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri598 – 623C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri659 – 683C2H2-type 2PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000003635
InParanoidiP32432
KOiK19487
OMAiLEKQYCK
OrthoDBiEOG092C4FS7

Family and domain databases

InterProiView protein in InterPro
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 2 hits
PS50157 ZINC_FINGER_C2H2_2, 2 hits

Sequencei

Sequence statusi: Complete.

P32432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFTTMTMAS NMATSTTTTA TSAHASINSS SNFNIDIDSN QNTPSILINN
60 70 80 90 100
NSDSSNGKNT DFNGVNNIHQ KNIMNNTNNV HLYSPNIMDQ TLLTPQDIAK
110 120 130 140 150
LRRESIAHSQ GMGGVSWGSI SVGSWLRDEI ISRRNSIVPA SANGAASAAA
160 170 180 190 200
SATTTATNTL QIQQPTKRPS VSNPPYHRGY SISPQIAYTA YLPNLEKQYC
210 220 230 240 250
KDYSCCGLSL PGLHDLLRHY EEAHISTSPN TTNMSQIPMN SAGNTSSSVR
260 270 280 290 300
MTNNTSSANY NLQNNMAANT KNAGHKTNTM QAHSSNATNN TSINNMHANL
310 320 330 340 350
QSNMDSNSTI RQSQHPHHQQ NIIQQQLQSN SVNHTSGAVP TPSVMGSATA
360 370 380 390 400
SSTTANPNVI SITGAPNSGL SMANHSQQLH LNGNLVDAVS TNDVFLRTSN
410 420 430 440 450
SPSRHVPHNK QINSNNNSGI NINNNTSHNS NINMGSKNAM VNRPHTFNNY
460 470 480 490 500
SLNKTSRNPI QHQSRKIDPH QTDLSPLVLV QDIDLSFMDD DILGPSNHNS
510 520 530 540 550
MNSVVNPTTG SHNYNTFHSS VHAKSSQNMV EDQDIDDIDD DDDVDDDDDD
560 570 580 590 600
DDDDDTENGS SSNGKSVHNN NYKMPQQAYI DDPARRLYVM DHEEQKPFKC
610 620 630 640 650
PVIGCEKTYK NQNGLKYHRL HGHQNQKLHE NPDGTFSVID PDSTDSFGDG
660 670 680
MGSAKDKPYR CEVCGKRYKN LNGLKYHRGH STH
Length:683
Mass (Da):74,785
Last modified:October 1, 1993 - v1
Checksum:i0332A381D75A6F14
GO

Sequence cautioni

The sequence AAA35041 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63577 Genomic DNA Translation: AAA35041.1 Different initiation.
U19729 Genomic DNA Translation: AAB82343.1
BK006945 Genomic DNA Translation: DAA09702.1
PIRiJH0497
RefSeqiNP_013507.1, NM_001182291.1

Genome annotation databases

EnsemblFungiiYLR403W; YLR403W; YLR403W
GeneIDi851119
KEGGisce:YLR403W

Similar proteinsi

Entry informationi

Entry nameiSFP1_YEAST
AccessioniPrimary (citable) accession number: P32432
Secondary accession number(s): D6VZ36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 20, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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