UniProtKB - P32432 (SFP1_YEAST)
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Protein
Transcription factor SFP1
Gene
SFP1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Transcription factor that regulates ribosomal protein (RP) and ribosome biogenesis (Ribi) gene expression in response to nutrients and stress. Promotes RP gene expression under optimal growth conditions. Leaves the nucleus upon environmental challenges, resulting in a down-regulation of RP gene transcription. The effect of the environmental cues on SFP1 localization is mediated through the TOR pathway. Also regulates the expression of genes involved in the G2/M transition during the mitotic cell cycle and the DNA-damage response. Required for carbon-source modulation of cell size.5 Publications
Miscellaneous
[ISP+] is the prion form of SFP1. [ISP+] is the result of a conformational change of the cellular SFP1 protein that becomes self-propagating and infectious. This conformational change generates a form of SFP1 that assembles into amyloid fibrils. [ISP+]-aggregates accumulate in the nucleus, and results in significantly larger cell size and increased drug resistance (PubMed:20498075). [ISP+] can be cured by GdnHCl (PubMed:11805042). It is speculated that prion properties of transcription factors may generate an optimized phenotypic heterogeneity that buffers yeast populations against diverse environmental insults (PubMed:20498075).2 Publications
Present with 259 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 598 – 623 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 659 – 683 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- positive regulation of ribosomal protein gene transcription by RNA polymerase II Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
- regulation of cell size Source: SGD
- transcription, DNA-templated Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, Prion |
Biological process | Stress response, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | YEAST:G3O-32465-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor SFP1Alternative name(s): Split finger protein 1 |
Gene namesi | Name:SFP1 Ordered Locus Names:YLR403W ORF Names:L8084.4 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YLR403W. |
SGDi | S000004395. SFP1. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046851 | 1 – 683 | Transcription factor SFP1Add BLAST | 683 |
Post-translational modificationi
Phosphorylated by TORC1 kinase at multiple sites. Phosphorylation regulates nuclear localization and RP promoter binding.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P32432. |
PaxDbi | P32432. |
PRIDEi | P32432. |
PTM databases
iPTMneti | P32432. |
Interactioni
Subunit structurei
Interacts with the target of rapamycin complex 1 (TORC1) in a rapamycin-dependent manner. Interacts with MRS6.1 Publication
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
MRS6 | P32864 | 5 | EBI-17035,EBI-14799 |
Protein-protein interaction databases
BioGridi | 31660. 52 interactors. |
DIPi | DIP-1379N. |
IntActi | P32432. 25 interactors. |
MINTi | P32432. |
STRINGi | 4932.YLR403W. |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 230 – 458 | Prion domain (PrD)Add BLAST | 229 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 532 – 555 | Poly-AspAdd BLAST | 24 |
Domaini
The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 598 – 623 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 659 – 683 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
GeneTreei | ENSGT00390000003635. |
InParanoidi | P32432. |
KOi | K19487. |
OMAi | LEKQYCK. |
OrthoDBi | EOG092C4FS7. |
Family and domain databases
InterProi | View protein in InterPro IPR036236. Znf_C2H2_sf. IPR013087. Znf_C2H2_type. |
SMARTi | View protein in SMART SM00355. ZnF_C2H2. 3 hits. |
SUPFAMi | SSF57667. SSF57667. 2 hits. |
PROSITEi | View protein in PROSITE PS00028. ZINC_FINGER_C2H2_1. 2 hits. PS50157. ZINC_FINGER_C2H2_2. 2 hits. |
i Sequence
Sequence statusi: Complete.
P32432-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDFTTMTMAS NMATSTTTTA TSAHASINSS SNFNIDIDSN QNTPSILINN
60 70 80 90 100
NSDSSNGKNT DFNGVNNIHQ KNIMNNTNNV HLYSPNIMDQ TLLTPQDIAK
110 120 130 140 150
LRRESIAHSQ GMGGVSWGSI SVGSWLRDEI ISRRNSIVPA SANGAASAAA
160 170 180 190 200
SATTTATNTL QIQQPTKRPS VSNPPYHRGY SISPQIAYTA YLPNLEKQYC
210 220 230 240 250
KDYSCCGLSL PGLHDLLRHY EEAHISTSPN TTNMSQIPMN SAGNTSSSVR
260 270 280 290 300
MTNNTSSANY NLQNNMAANT KNAGHKTNTM QAHSSNATNN TSINNMHANL
310 320 330 340 350
QSNMDSNSTI RQSQHPHHQQ NIIQQQLQSN SVNHTSGAVP TPSVMGSATA
360 370 380 390 400
SSTTANPNVI SITGAPNSGL SMANHSQQLH LNGNLVDAVS TNDVFLRTSN
410 420 430 440 450
SPSRHVPHNK QINSNNNSGI NINNNTSHNS NINMGSKNAM VNRPHTFNNY
460 470 480 490 500
SLNKTSRNPI QHQSRKIDPH QTDLSPLVLV QDIDLSFMDD DILGPSNHNS
510 520 530 540 550
MNSVVNPTTG SHNYNTFHSS VHAKSSQNMV EDQDIDDIDD DDDVDDDDDD
560 570 580 590 600
DDDDDTENGS SSNGKSVHNN NYKMPQQAYI DDPARRLYVM DHEEQKPFKC
610 620 630 640 650
PVIGCEKTYK NQNGLKYHRL HGHQNQKLHE NPDGTFSVID PDSTDSFGDG
660 670 680
MGSAKDKPYR CEVCGKRYKN LNGLKYHRGH STH
Sequence cautioni
The sequence AAA35041 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63577 Genomic DNA. Translation: AAA35041.1. Different initiation. U19729 Genomic DNA. Translation: AAB82343.1. BK006945 Genomic DNA. Translation: DAA09702.1. |
PIRi | JH0497. |
RefSeqi | NP_013507.1. NM_001182291.1. |
Genome annotation databases
EnsemblFungii | YLR403W; YLR403W; YLR403W. |
GeneIDi | 851119. |
KEGGi | sce:YLR403W. |
Similar proteinsi
Entry informationi
Entry namei | SFP1_YEAST | |
Accessioni | P32432Primary (citable) accession number: P32432 Secondary accession number(s): D6VZ36 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | March 28, 2018 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Caution
It is uncertain whether Met-1 or Met-6 is the initiator.Curated