P32427 (PCAB_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-carboxy-cis,cis-muconate cycloisomerase
EC=5.5.1.2

Alternative name(s):
3-carboxymuconate lactonizing enzyme
Short name=CMLE

Gene names

Name:

pcaB

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group

Protein attributes

Sequence length	422 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes an anti cycloisomerization.
Catalytic activity	2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate.
Pathway	Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-4,5-dihydro-2-furylacetate from 3-carboxy-cis,cis-muconate: step 1/2.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II fumarase/aspartase family.
Sequence caution	The sequence AAA25920.1 differs from that shown. Reason: Frameshift at position 379.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	beta-ketoadipate pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway protocatechuate catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-carboxy-cis,cis-muconate cycloisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›422	›422	3-carboxy-cis,cis-muconate cycloisomerase		PRO_0000161349
Experimental info
	Non-terminal residue	422	1

Sequences

	Sequence		Length	Mass (Da)
	P32427 [UniParc]. Last modified May 15, 2007. Version 3. Checksum: DCB0C1E9E90DB03B	FASTA	422	44,676
	10 20 30 40 50 60 MTNQLFDAYF TAPAMREIFS DRGRLQGMLD FEAALARAEA AAGLVPHSAV AAIEAACKAE 70 80 90 100 110 120 RYDVGALANA IATAGNSAIP LVKALGKVIA SGVPEAERYV HLGATSQDAM DTGLVLQLRD 130 140 150 160 170 180 ALDLIEADLG KLADTLSQQA LKHADTPMVG RTWLQHATPV TLGMKLAGVL GALTRHRQRL 190 200 210 220 230 240 QELGPPCWCC SSGGASGSLA ALGSKAMPVA EALAEQLKLS LPEQPWHTQR DRLVEFASVL 250 260 270 280 290 300 GLVAGSLGKF GRDVSLLMQT EAGEVFEPSA PGKGGSSTMP HKRNPVGAAV LIGAATRVPG 310 320 330 340 350 360 LVSTLFAAMP QEHERSLGLW HAEWETLPDI CCLVSGALRQ AQVIAEGIEV DAARMRRNLD 370 380 390 400 410 420 LTQGLVLAEA VSIVLARTLG RDRAHHLLEQ CCQRAVAEQR HLRAVLGDDP QVSAELSAEE LD

References

[1]

"3-carboxy-cis,cis-muconate lactonizing enzyme from Pseudomonas putida is homologous to the class II fumarase family: a new reaction in the evolution of a mechanistic motif." Williams S.E., Woolridge E.M., Ransom S.C., Landro J.A., Babbitt P.C., Kozarich J.W. Biochemistry 31:9768-9776(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15. Strain: PRS2000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L17082 Genomic DNA. Translation: AAA25920.1. Frameshift.
PIR	A44374.
3D structure databases
ProteinModelPortal	P32427.
SMR	P32427. Positions 3-422.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-3222.
UniPathway	UPA00157; UER00265.
Family and domain databases
Gene3D	1.10.275.10. 1 hit.
InterPro	IPR019468. AdenyloSucc_lyase_C. IPR003031. D_crystallin. IPR024083. Fumarase/histidase_N. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR022761. Fumarate_lyase_N. IPR008948. L-Aspartase-like. IPR012789. Protocat_PcaB. [Graphical view]
Pfam	PF10397. ADSL_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SMART	SM00998. ADSL_C. 1 hit. [Graphical view]
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR02426. protocat_pcaB. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCAB_PSEPU

Accession

Primary (citable) accession number: P32427
Secondary accession number(s): Q59703

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	May 15, 2007
Last modified:	April 3, 2013
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program