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P32420

- SDHB_USTMA

UniProt

P32420 - SDHB_USTMA

Protein

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Gene

SDH2

Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity

    Catalytic activityi

    Succinate + a quinone = fumarate + a quinol.

    Cofactori

    Binds 1 2Fe-2S cluster.By similarity
    Binds 1 3Fe-4S cluster.By similarity
    Binds 1 4Fe-4S cluster.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi106 – 1061Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi111 – 1111Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi114 – 1141Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi126 – 1261Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi195 – 1951Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi198 – 1981Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi201 – 2011Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi205 – 2051Iron-sulfur 3 (3Fe-4S)By similarity
    Binding sitei210 – 2101Ubiquinone; shared with DHSDBy similarity
    Metal bindingi252 – 2521Iron-sulfur 3 (3Fe-4S)By similarity
    Metal bindingi258 – 2581Iron-sulfur 3 (3Fe-4S)By similarity
    Metal bindingi262 – 2621Iron-sulfur 2 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    4. electron carrier activity Source: InterPro
    5. metal ion binding Source: UniProtKB-KW
    6. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport, Tricarboxylic acid cycle

    Keywords - Ligandi

    2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00223; UER01006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC:1.3.5.1)
    Alternative name(s):
    Iron-sulfur subunit of complex II
    Short name:
    Ip
    Gene namesi
    Name:SDH2
    Synonyms:CBXR
    OrganismiUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
    Taxonomic identifieri237631 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago
    ProteomesiUP000000561: Unassembled WGS sequence

    Subcellular locationi

    Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 295Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrialPRO_0000010352
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Interactioni

    Subunit structurei

    Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.By similarity

    Protein-protein interaction databases

    STRINGi5270.UM00844.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP32420.
    SMRiP32420. Positions 52-280.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini67 – 144782Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini185 – 215314Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0479.
    HOGENOMiHOG000160590.
    KOiK00235.
    OMAiIMNCSRT.
    OrthoDBiEOG7X9GJ0.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR012675. Beta-grasp_dom.
    IPR009051. Helical_ferredxn.
    IPR004489. Succ_DH/fum_Rdtase_Fe-S.
    IPR025192. Succ_DH/fum_Rdtase_N.
    [Graphical view]
    PfamiPF13085. Fer2_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF46548. SSF46548. 1 hit.
    SSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR00384. dhsB. 1 hit.
    PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32420-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLFNVSNGL RTALRPSVAS SSRVAAFSTT AAARLATPTS DNVGSSGKPQ    50
    HLKQFKIYRW NPDKPSEKPR LQSYTLDLNQ TGPMVLDALI KIKNEIDPTL 100
    TFRRSCREGI CGSCAMNIDG VNTLACLCRI DKQNDTKIYP LPHMYIVKDL 150
    VPDLTQFYKQ YRSIEPFLKS NNTPSEGEHL QSPEERRRLD GLYECILCAC 200
    CSTSCPSYWW NQDEYLGPAV LMQAYRWMAD SRDDFGEERR QKLENTFSLY 250
    RCHTIMNCSR TCPKNLNPGK AIAQIKKDMA VGAPKASERP IMASS 295
    Length:295
    Mass (Da):33,282
    Last modified:October 1, 1993 - v1
    Checksum:iF374A20BA8F8E7E5
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti253 – 2531H → L in carboxin-resistant mutant.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62762 Genomic DNA. Translation: CAA44612.1.
    Z11738 Genomic DNA. Translation: CAA77798.1.
    AACP01000027 Genomic DNA. Translation: EAK81918.1.
    PIRiS26978.
    RefSeqiXP_756991.1. XM_751898.1.

    Genome annotation databases

    EnsemblFungiiUM00844T0; UM00844P0; UM00844.
    GeneIDi3628909.
    KEGGiuma:UM00844.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62762 Genomic DNA. Translation: CAA44612.1 .
    Z11738 Genomic DNA. Translation: CAA77798.1 .
    AACP01000027 Genomic DNA. Translation: EAK81918.1 .
    PIRi S26978.
    RefSeqi XP_756991.1. XM_751898.1.

    3D structure databases

    ProteinModelPortali P32420.
    SMRi P32420. Positions 52-280.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5270.UM00844.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii UM00844T0 ; UM00844P0 ; UM00844 .
    GeneIDi 3628909.
    KEGGi uma:UM00844.1.

    Phylogenomic databases

    eggNOGi COG0479.
    HOGENOMi HOG000160590.
    KOi K00235.
    OMAi IMNCSRT.
    OrthoDBi EOG7X9GJ0.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01006 .

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR012675. Beta-grasp_dom.
    IPR009051. Helical_ferredxn.
    IPR004489. Succ_DH/fum_Rdtase_Fe-S.
    IPR025192. Succ_DH/fum_Rdtase_N.
    [Graphical view ]
    Pfami PF13085. Fer2_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46548. SSF46548. 1 hit.
    SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR00384. dhsB. 1 hit.
    PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, characterization and sequence of a gene conferring resistance to the systemic fungicide carboxin from the maize smut pathogen, Ustilago maydis."
      Keon J.P.R., White G.A., Hargreaves J.A.
      Curr. Genet. 19:475-481(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 38509 / 92.
    2. "A single amino-acid change in the iron-sulphur protein subunit of succinate dehydrogenase confers resistance to carboxin in Ustilago maydis."
      Broomfield P.L.E., Hargreaves J.A.
      Curr. Genet. 22:117-121(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis."
      Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J.
      , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W.
      Nature 444:97-101(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 521 / FGSC 9021.

    Entry informationi

    Entry nameiSDHB_USTMA
    AccessioniPrimary (citable) accession number: P32420
    Secondary accession number(s): Q12722, Q4PGB9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3