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P32420

- SDHB_USTMA

UniProt

P32420 - SDHB_USTMA

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Protein

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Gene

SDH2

Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi106 – 1061Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi111 – 1111Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi114 – 1141Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi126 – 1261Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi195 – 1951Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi198 – 1981Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi201 – 2011Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi205 – 2051Iron-sulfur 3 (3Fe-4S)By similarity
Binding sitei210 – 2101Ubiquinone; shared with DHSDBy similarity
Metal bindingi252 – 2521Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi258 – 2581Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi262 – 2621Iron-sulfur 2 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  4. electron carrier activity Source: InterPro
  5. metal ion binding Source: UniProtKB-KW
  6. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Iron-sulfur subunit of complex II
Short name:
Ip
Gene namesi
Name:SDH2
Synonyms:CBXR
OrganismiUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Taxonomic identifieri237631 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago
ProteomesiUP000000561: Unassembled WGS sequence

Subcellular locationi

Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 295Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrialPRO_0000010352
Transit peptidei1 – ?MitochondrionSequence Analysis

Interactioni

Subunit structurei

Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.By similarity

Protein-protein interaction databases

STRINGi5270.UM00844.1.

Structurei

3D structure databases

ProteinModelPortaliP32420.
SMRiP32420. Positions 52-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 144782Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini185 – 215314Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0479.
HOGENOMiHOG000160590.
InParanoidiP32420.
KOiK00235.
OMAiIMNCSRT.
OrthoDBiEOG7X9GJ0.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32420-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLFNVSNGL RTALRPSVAS SSRVAAFSTT AAARLATPTS DNVGSSGKPQ
60 70 80 90 100
HLKQFKIYRW NPDKPSEKPR LQSYTLDLNQ TGPMVLDALI KIKNEIDPTL
110 120 130 140 150
TFRRSCREGI CGSCAMNIDG VNTLACLCRI DKQNDTKIYP LPHMYIVKDL
160 170 180 190 200
VPDLTQFYKQ YRSIEPFLKS NNTPSEGEHL QSPEERRRLD GLYECILCAC
210 220 230 240 250
CSTSCPSYWW NQDEYLGPAV LMQAYRWMAD SRDDFGEERR QKLENTFSLY
260 270 280 290
RCHTIMNCSR TCPKNLNPGK AIAQIKKDMA VGAPKASERP IMASS
Length:295
Mass (Da):33,282
Last modified:October 1, 1993 - v1
Checksum:iF374A20BA8F8E7E5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti253 – 2531H → L in carboxin-resistant mutant.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62762 Genomic DNA. Translation: CAA44612.1.
Z11738 Genomic DNA. Translation: CAA77798.1.
AACP01000027 Genomic DNA. Translation: EAK81918.1.
PIRiS26978.
RefSeqiXP_756991.1. XM_751898.1.

Genome annotation databases

EnsemblFungiiUM00844T0; UM00844P0; UM00844.
GeneIDi3628909.
KEGGiuma:UM00844.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62762 Genomic DNA. Translation: CAA44612.1 .
Z11738 Genomic DNA. Translation: CAA77798.1 .
AACP01000027 Genomic DNA. Translation: EAK81918.1 .
PIRi S26978.
RefSeqi XP_756991.1. XM_751898.1.

3D structure databases

ProteinModelPortali P32420.
SMRi P32420. Positions 52-280.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5270.UM00844.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii UM00844T0 ; UM00844P0 ; UM00844 .
GeneIDi 3628909.
KEGGi uma:UM00844.1.

Phylogenomic databases

eggNOGi COG0479.
HOGENOMi HOG000160590.
InParanoidi P32420.
KOi K00235.
OMAi IMNCSRT.
OrthoDBi EOG7X9GJ0.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01006 .

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view ]
Pfami PF13085. Fer2_3. 1 hit.
[Graphical view ]
SUPFAMi SSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR00384. dhsB. 1 hit.
PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, characterization and sequence of a gene conferring resistance to the systemic fungicide carboxin from the maize smut pathogen, Ustilago maydis."
    Keon J.P.R., White G.A., Hargreaves J.A.
    Curr. Genet. 19:475-481(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 38509 / 92.
  2. "A single amino-acid change in the iron-sulphur protein subunit of succinate dehydrogenase confers resistance to carboxin in Ustilago maydis."
    Broomfield P.L.E., Hargreaves J.A.
    Curr. Genet. 22:117-121(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis."
    Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J.
    , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W.
    Nature 444:97-101(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 521 / FGSC 9021.

Entry informationi

Entry nameiSDHB_USTMA
AccessioniPrimary (citable) accession number: P32420
Secondary accession number(s): Q12722, Q4PGB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3