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Protein

Malate dehydrogenase, peroxisomal

Gene

MDH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3).
Present with 3300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADBy similarity1
Binding sitei80SubstratePROSITE-ProRule annotation1
Binding sitei86SubstratePROSITE-ProRule annotation1
Binding sitei93NADBy similarity1
Binding sitei118SubstratePROSITE-ProRule annotation1
Binding sitei152SubstratePROSITE-ProRule annotation1
Active sitei187Proton acceptorBy similarity1
Binding sitei237NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 14NADBy similarity7
Nucleotide bindingi116 – 118NADBy similarity3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: SGD
  • mRNA binding Source: SGD

GO - Biological processi

  • fatty acid beta-oxidation Source: SGD
  • glyoxylate cycle Source: UniProtKB-KW
  • malate metabolic process Source: InterPro
  • NADH regeneration Source: SGD
  • tricarboxylic acid cycle Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciYEAST:YDL078C-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, peroxisomal (EC:1.1.1.37)
Gene namesi
Name:MDH3
Ordered Locus Names:YDL078C
ORF Names:D2468
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL078C
SGDiS000002236 MDH3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001133422 – 343Malate dehydrogenase, peroxisomalAdd BLAST342

Proteomic databases

MaxQBiP32419
PaxDbiP32419
PRIDEiP32419

2D gel databases

UCD-2DPAGEiP32419

PTM databases

iPTMnetiP32419

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi31983, 152 interactors
DIPiDIP-6473N
IntActiP32419, 7 interactors
MINTiP32419
STRINGi4932.YDL078C

Structurei

3D structure databases

ProteinModelPortaliP32419
SMRiP32419
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075847
HOGENOMiHOG000213792
InParanoidiP32419
KOiK00026
OMAiFCIVTNP
OrthoDBiEOG092C34N7

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32419-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKVAILGAS GGVGQPLSLL LKLSPYVSEL ALYDIRAAEG IGKDLSHINT
60 70 80 90 100
NSSCVGYDKD SIENTLSNAQ VVLIPAGVPR KPGLTRDDLF KMNAGIVKSL
110 120 130 140 150
VTAVGKFAPN ARILVISNPV NSLVPIAVET LKKMGKFKPG NVMGVTNLDL
160 170 180 190 200
VRAETFLVDY LMLKNPKIGQ EQDKTTMHRK VTVIGGHSGE TIIPIITDKS
210 220 230 240 250
LVFQLDKQYE HFIHRVQFGG DEIVKAKQGA GSATLSMAFA GAKFAEEVLR
260 270 280 290 300
SFHNEKPETE SLSAFVYLPG LKNGKKAQQL VGDNSIEYFS LPIVLRNGSV
310 320 330 340
VSIDTSVLEK LSPREEQLVN TAVKELRKNI EKGKSFILDS SKL
Length:343
Mass (Da):37,186
Last modified:January 23, 2007 - v3
Checksum:i54725114B2CAD6A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti240A → R in AAA34767 (PubMed:1447211).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98763 Genomic DNA Translation: AAA34767.1
Z74126 Genomic DNA Translation: CAA98644.1
BK006938 Genomic DNA Translation: DAA11781.1
PIRiS67614 DEBYMP
RefSeqiNP_010205.1, NM_001180137.1

Genome annotation databases

EnsemblFungiiYDL078C; YDL078C; YDL078C
GeneIDi851481
KEGGisce:YDL078C

Similar proteinsi

Entry informationi

Entry nameiMDHP_YEAST
AccessioniPrimary (citable) accession number: P32419
Secondary accession number(s): D6VRS1, Q12689
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 163 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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