P32418 (NAC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/calcium exchanger 1

Alternative name(s):
Na(+)/Ca(2+)-exchange protein 1
Solute carrier family 8 member 1

Gene names

Name:	SLC8A1
Synonyms:	CNC, NCX1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	973 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Rapidly transports Ca2+ during excitation-contraction coupling. Ca2+ is extruded from the cell during relaxation so as to prevent overloading of intracellular stores. Ref.11
Enzyme regulation	By ATP.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in cardiac sarcolemma, brain, kidney, liver, pancreas, skeletal muscle, placenta and lung. Ref.11
Sequence similarities	Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily. Contains 2 Calx-beta domains.
Sequence caution	The sequence AAH98308.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Antiport Calcium transport Ion transport Sodium transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium Calmodulin-binding Metal-binding Sodium
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome calcium ion export Inferred from direct assay PubMed 19481548. Source: BHF-UCL calcium ion homeostasis Inferred from sequence or structural similarity. Source: BHF-UCL calcium ion import Inferred from direct assay Ref.1PubMed 19481548PubMed 19481548. Source: BHF-UCL calcium ion transport into cytosol Inferred from sequence or structural similarity. Source: BHF-UCL cardiac muscle cell development Inferred from sequence or structural similarity. Source: BHF-UCL cardiac muscle contraction Inferred from electronic annotation. Source: Ensembl cell communication by electrical coupling involved in cardiac conduction Inferred from sequence or structural similarity. Source: BHF-UCL cellular response to caffeine Inferred from sequence or structural similarity. Source: BHF-UCL cellular response to reactive oxygen species Inferred from direct assay PubMed 19481548PubMed 19481548. Source: BHF-UCL cytosolic calcium ion transport Traceable author statement PubMed 19095005. Source: BHF-UCL embryonic heart tube development Inferred from electronic annotation. Source: Ensembl embryonic placenta development Inferred from electronic annotation. Source: Ensembl heart morphogenesis Inferred from electronic annotation. Source: Ensembl ion transport Traceable author statement. Source: Reactome muscle contraction Traceable author statement Ref.1. Source: ProtInc muscle fiber development Inferred from electronic annotation. Source: Ensembl negative regulation of cytosolic calcium ion concentration Inferred from sequence or structural similarity. Source: BHF-UCL post-embryonic development Inferred from electronic annotation. Source: Ensembl regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion Inferred from sequence or structural similarity. Source: BHF-UCL regulation of heart rate Inferred from sequence or structural similarity. Source: BHF-UCL regulation of the force of heart contraction Inferred from sequence or structural similarity. Source: BHF-UCL relaxation of cardiac muscle Inferred by curator PubMed 19481548. Source: BHF-UCL relaxation of smooth muscle Inferred from sequence or structural similarity. Source: BHF-UCL sodium ion export Inferred from direct assay PubMed 19481548PubMed 19481548. Source: BHF-UCL sodium ion import Inferred from direct assay Ref.1. Source: BHF-UCL transmembrane transport Traceable author statement. Source: Reactome vascular smooth muscle contraction Inferred from sequence or structural similarity. Source: BHF-UCL
Cellular_component	T-tubule Inferred from sequence or structural similarity. Source: BHF-UCL Z disc Inferred from sequence or structural similarity. Source: BHF-UCL basolateral plasma membrane Inferred from electronic annotation. Source: Ensembl integral component of plasma membrane Inferred from direct assay Ref.1. Source: BHF-UCL intercalated disc Inferred from sequence or structural similarity. Source: BHF-UCL plasma membrane Traceable author statement PubMed 19095005. Source: BHF-UCL sarcolemma Inferred from sequence or structural similarity. Source: BHF-UCL
Molecular_function	ankyrin binding Inferred from physical interaction PubMed 17178715. Source: BHF-UCL calcium:sodium antiporter activity Inferred from direct assay Ref.1PubMed 19481548PubMed 19481548. Source: BHF-UCL cytoskeletal protein binding Inferred from direct assay PubMed 16292983. Source: BHF-UCL ion channel binding Inferred from sequence or structural similarity. Source: BHF-UCL metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P32418-1)

Also known as: NaCa1; NCX1.1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 3 (identifier: P32418-2)

Also known as: NaCa3; NCX1.3;

The sequence of this isoform differs from the canonical sequence as follows:
     605-613: TISVKVIDD → IITIRIFDR
     619-645: NKTFFLEIGEPRLVEMSEKKALLLNEL → ECSFSLVLEEPKWIRRGMK
     652-679: Missing.

Isoform 7 (identifier: P32418-3)

Also known as: NaCa7; NCX1.7;

The sequence of this isoform differs from the canonical sequence as follows:
     605-613: TISVKVIDD → IITIRIFDR
     619-645: NKTFFLEIGEPRLVEMSEKKALLLNEL → ECSFSLVLEEPKWIRRGMK
     652-656: Missing.

Isoform 10 (identifier: P32418-4)

Also known as: NaCa10; NCX1.10;

The sequence of this isoform differs from the canonical sequence as follows:
     605-613: TISVKVIDD → IITIRIFDR
     619-645: NKTFFLEIGEPRLVEMSEKKALLLNEL → ECSFSLVLEEPKWIRRGMK

Isoform 5 (identifier: P32418-5)

The sequence of this isoform differs from the canonical sequence as follows:
     652-656: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 973	938	Sodium/calcium exchanger 1		PRO_0000019379
Regions
	Topological domain	36 – 74	39	Extracellular Potential
	Transmembrane	75 – 95	21	Helical; Potential
	Topological domain	96 – 136	41	Cytoplasmic Potential
	Transmembrane	137 – 157	21	Helical; Potential
	Topological domain	158 – 170	13	Extracellular Potential
	Transmembrane	171 – 191	21	Helical; Potential
	Topological domain	192 – 204	13	Cytoplasmic Potential
	Transmembrane	205 – 225	21	Helical; Potential
	Topological domain	226 – 231	6	Extracellular Potential
	Transmembrane	232 – 252	21	Helical; Potential
	Topological domain	253 – 800	548	Cytoplasmic Potential
	Transmembrane	801 – 821	21	Helical; Potential
	Topological domain	822 – 824	3	Extracellular Potential
	Transmembrane	825 – 845	21	Helical; Potential
	Topological domain	846 – 874	29	Cytoplasmic Potential
	Transmembrane	875 – 895	21	Helical; Potential
	Topological domain	896 – 906	11	Extracellular Potential
	Transmembrane	907 – 927	21	Helical; Potential
	Topological domain	928 – 944	17	Cytoplasmic Potential
	Transmembrane	945 – 965	21	Helical; Potential
	Topological domain	966 – 973	8	Extracellular Potential
	Repeat	141 – 181	41	Alpha-1
	Domain	396 – 496	101	Calx-beta 1
	Domain	527 – 627	101	Calx-beta 2
	Repeat	842 – 878	37	Alpha-2
	Region	254 – 273	20	Calmodulin-binding Potential
	Compositional bias	239 – 242	4	Poly-Phe
	Compositional bias	692 – 695	4	Poly-Glu
	Compositional bias	759 – 763	5	Poly-Asp
Sites
	Metal binding	420	1	Calcium 3 By similarity
	Metal binding	456	1	Calcium 1 By similarity
	Metal binding	456	1	Calcium 2 By similarity
	Metal binding	481	1	Calcium 4 By similarity
	Metal binding	482	1	Calcium 3 By similarity
	Metal binding	482	1	Calcium 4; via carbonyl oxygen By similarity
	Metal binding	484	1	Calcium 3; via carbonyl oxygen By similarity
	Metal binding	486	1	Calcium 1 By similarity
	Metal binding	486	1	Calcium 2 By similarity
	Metal binding	486	1	Calcium 3 By similarity
	Metal binding	489	1	Calcium 1 By similarity
	Metal binding	533	1	Calcium 3 By similarity
	Metal binding	534	1	Calcium 4 By similarity
	Metal binding	535	1	Calcium 3 By similarity
	Metal binding	535	1	Calcium 4 By similarity
	Metal binding	551	1	Calcium 6 By similarity
	Metal binding	587	1	Calcium 5 By similarity
	Metal binding	613	1	Calcium 5 By similarity
	Metal binding	613	1	Calcium 6 By similarity
	Metal binding	615	1	Calcium 6 By similarity
	Metal binding	683	1	Calcium 6 By similarity
Amino acid modifications
	Modified residue	392	1	Phosphoserine Potential
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	160	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	605 – 613	9	TISVKVIDD → IITIRIFDR in isoform 3, isoform 7 and isoform 10.		VSP_003397
	Alternative sequence	619 – 645	27	NKTFF…LLNEL → ECSFSLVLEEPKWIRRGMK in isoform 3, isoform 7 and isoform 10.		VSP_003398
	Alternative sequence	652 – 679	28	Missing in isoform 3.		VSP_003400
	Alternative sequence	652 – 656	5	Missing in isoform 7 and isoform 5.		VSP_003399
	Natural variant	692	1	E → V. Corresponds to variant rs5557 [ dbSNP | Ensembl ].		VAR_014847

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (NaCa1) (NCX1.1) [UniParc]. Last modified December 1, 2000. Version 3. Checksum: 17DFC1B1F15921D8	FASTA	973	108,547
	10 20 30 40 50 60 MYNMRRLSLS PTFSMGFHLL VTVSLLFSHV DHVIAETEME GEGNETGECT GSYYCKKGVI 70 80 90 100 110 120 LPIWEPQDPS FGDKIARATV YFVAMVYMFL GVSIIADRFM SSIEVITSQE KEITIKKPNG 130 140 150 160 170 180 ETTKTTVRIW NETVSNLTLM ALGSSAPEIL LSVIEVCGHN FTAGDLGPST IVGSAAFNMF 190 200 210 220 230 240 IIIALCVYVV PDGETRKIKH LRVFFVTAAW SIFAYTWLYI ILSVISPGVV EVWEGLLTFF 250 260 270 280 290 300 FFPICVVFAW VADRRLLFYK YVYKRYRAGK QRGMIIEHEG DRPSSKTEIE MDGKVVNSHV 310 320 330 340 350 360 ENFLDGALVL EVDERDQDDE EARREMARIL KELKQKHPDK EIEQLIELAN YQVLSQQQKS 370 380 390 400 410 420 RAFYRIQATR LMTGAGNILK RHAADQARKA VSMHEVNTEV TENDPVSKIF FEQGTYQCLE 430 440 450 460 470 480 NCGTVALTII RRGGDLTNTV FVDFRTEDGT ANAGSDYEFT EGTVVFKPGD TQKEIRVGII 490 500 510 520 530 540 DDDIFEEDEN FLVHLSNVKV SSEASEDGIL EANHVSTLAC LGSPSTATVT IFDDDHAGIF 550 560 570 580 590 600 TFEEPVTHVS ESIGIMEVKV LRTSGARGNV IVPYKTIEGT ARGGGEDFED TCGELEFQND 610 620 630 640 650 660 EIVKTISVKV IDDEEYEKNK TFFLEIGEPR LVEMSEKKAL LLNELGGFTI TGKYLFGQPV 670 680 690 700 710 720 FRKVHAREHP ILSTVITIAD EYDDKQPLTS KEEEERRIAE MGRPILGEHT KLEVIIEESY 730 740 750 760 770 780 EFKSTVDKLI KKTNLALVVG TNSWREQFIE AITVSAGEDD DDDECGEEKL PSCFDYVMHF 790 800 810 820 830 840 LTVFWKVLFA FVPPTEYWNG WACFIVSILM IGLLTAFIGD LASHFGCTIG LKDSVTAVVF 850 860 870 880 890 900 VALGTSVPDT FASKVAATQD QYADASIGNV TGSNAVNVFL GIGVAWSIAA IYHAANGEQF 910 920 930 940 950 960 KVSPGTLAFS VTLFTIFAFI NVGVLLYRRR PEIGGELGGP RTAKLLTSCL FVLLWLLYIF 970 FSSLEAYCHI KGF
	Isoform 3 (NaCa3) (NCX1.3) [UniParc]. Checksum: 112BE915D7AE8723	FASTA	937	104,689
	10 20 30 40 50 60 MYNMRRLSLS PTFSMGFHLL VTVSLLFSHV DHVIAETEME GEGNETGECT GSYYCKKGVI 70 80 90 100 110 120 LPIWEPQDPS FGDKIARATV YFVAMVYMFL GVSIIADRFM SSIEVITSQE KEITIKKPNG 130 140 150 160 170 180 ETTKTTVRIW NETVSNLTLM ALGSSAPEIL LSVIEVCGHN FTAGDLGPST IVGSAAFNMF 190 200 210 220 230 240 IIIALCVYVV PDGETRKIKH LRVFFVTAAW SIFAYTWLYI ILSVISPGVV EVWEGLLTFF 250 260 270 280 290 300 FFPICVVFAW VADRRLLFYK YVYKRYRAGK QRGMIIEHEG DRPSSKTEIE MDGKVVNSHV 310 320 330 340 350 360 ENFLDGALVL EVDERDQDDE EARREMARIL KELKQKHPDK EIEQLIELAN YQVLSQQQKS 370 380 390 400 410 420 RAFYRIQATR LMTGAGNILK RHAADQARKA VSMHEVNTEV TENDPVSKIF FEQGTYQCLE 430 440 450 460 470 480 NCGTVALTII RRGGDLTNTV FVDFRTEDGT ANAGSDYEFT EGTVVFKPGD TQKEIRVGII 490 500 510 520 530 540 DDDIFEEDEN FLVHLSNVKV SSEASEDGIL EANHVSTLAC LGSPSTATVT IFDDDHAGIF 550 560 570 580 590 600 TFEEPVTHVS ESIGIMEVKV LRTSGARGNV IVPYKTIEGT ARGGGEDFED TCGELEFQND 610 620 630 640 650 660 EIVKIITIRI FDREEYEKEC SFSLVLEEPK WIRRGMKGGF TITDEYDDKQ PLTSKEEEER 670 680 690 700 710 720 RIAEMGRPIL GEHTKLEVII EESYEFKSTV DKLIKKTNLA LVVGTNSWRE QFIEAITVSA 730 740 750 760 770 780 GEDDDDDECG EEKLPSCFDY VMHFLTVFWK VLFAFVPPTE YWNGWACFIV SILMIGLLTA 790 800 810 820 830 840 FIGDLASHFG CTIGLKDSVT AVVFVALGTS VPDTFASKVA ATQDQYADAS IGNVTGSNAV 850 860 870 880 890 900 NVFLGIGVAW SIAAIYHAAN GEQFKVSPGT LAFSVTLFTI FAFINVGVLL YRRRPEIGGE 910 920 930 LGGPRTAKLL TSCLFVLLWL LYIFFSSLEA YCHIKGF
	Isoform 7 (NaCa7) (NCX1.7) [UniParc]. Checksum: 85F0A9CD7BE0A806	FASTA	960	107,242
	10 20 30 40 50 60 MYNMRRLSLS PTFSMGFHLL VTVSLLFSHV DHVIAETEME GEGNETGECT GSYYCKKGVI 70 80 90 100 110 120 LPIWEPQDPS FGDKIARATV YFVAMVYMFL GVSIIADRFM SSIEVITSQE KEITIKKPNG 130 140 150 160 170 180 ETTKTTVRIW NETVSNLTLM ALGSSAPEIL LSVIEVCGHN FTAGDLGPST IVGSAAFNMF 190 200 210 220 230 240 IIIALCVYVV PDGETRKIKH LRVFFVTAAW SIFAYTWLYI ILSVISPGVV EVWEGLLTFF 250 260 270 280 290 300 FFPICVVFAW VADRRLLFYK YVYKRYRAGK QRGMIIEHEG DRPSSKTEIE MDGKVVNSHV 310 320 330 340 350 360 ENFLDGALVL EVDERDQDDE EARREMARIL KELKQKHPDK EIEQLIELAN YQVLSQQQKS 370 380 390 400 410 420 RAFYRIQATR LMTGAGNILK RHAADQARKA VSMHEVNTEV TENDPVSKIF FEQGTYQCLE 430 440 450 460 470 480 NCGTVALTII RRGGDLTNTV FVDFRTEDGT ANAGSDYEFT EGTVVFKPGD TQKEIRVGII 490 500 510 520 530 540 DDDIFEEDEN FLVHLSNVKV SSEASEDGIL EANHVSTLAC LGSPSTATVT IFDDDHAGIF 550 560 570 580 590 600 TFEEPVTHVS ESIGIMEVKV LRTSGARGNV IVPYKTIEGT ARGGGEDFED TCGELEFQND 610 620 630 640 650 660 EIVKIITIRI FDREEYEKEC SFSLVLEEPK WIRRGMKGGF TITGQPVFRK VHAREHPILS 670 680 690 700 710 720 TVITIADEYD DKQPLTSKEE EERRIAEMGR PILGEHTKLE VIIEESYEFK STVDKLIKKT 730 740 750 760 770 780 NLALVVGTNS WREQFIEAIT VSAGEDDDDD ECGEEKLPSC FDYVMHFLTV FWKVLFAFVP 790 800 810 820 830 840 PTEYWNGWAC FIVSILMIGL LTAFIGDLAS HFGCTIGLKD SVTAVVFVAL GTSVPDTFAS 850 860 870 880 890 900 KVAATQDQYA DASIGNVTGS NAVNVFLGIG VAWSIAAIYH AANGEQFKVS PGTLAFSVTL 910 920 930 940 950 960 FTIFAFINVG VLLYRRRPEI GGELGGPRTA KLLTSCLFVL LWLLYIFFSS LEAYCHIKGF
	Isoform 10 (NaCa10) (NCX1.10) [UniParc]. Checksum: FB6516EB3925B431	FASTA	965	107,850
	10 20 30 40 50 60 MYNMRRLSLS PTFSMGFHLL VTVSLLFSHV DHVIAETEME GEGNETGECT GSYYCKKGVI 70 80 90 100 110 120 LPIWEPQDPS FGDKIARATV YFVAMVYMFL GVSIIADRFM SSIEVITSQE KEITIKKPNG 130 140 150 160 170 180 ETTKTTVRIW NETVSNLTLM ALGSSAPEIL LSVIEVCGHN FTAGDLGPST IVGSAAFNMF 190 200 210 220 230 240 IIIALCVYVV PDGETRKIKH LRVFFVTAAW SIFAYTWLYI ILSVISPGVV EVWEGLLTFF 250 260 270 280 290 300 FFPICVVFAW VADRRLLFYK YVYKRYRAGK QRGMIIEHEG DRPSSKTEIE MDGKVVNSHV 310 320 330 340 350 360 ENFLDGALVL EVDERDQDDE EARREMARIL KELKQKHPDK EIEQLIELAN YQVLSQQQKS 370 380 390 400 410 420 RAFYRIQATR LMTGAGNILK RHAADQARKA VSMHEVNTEV TENDPVSKIF FEQGTYQCLE 430 440 450 460 470 480 NCGTVALTII RRGGDLTNTV FVDFRTEDGT ANAGSDYEFT EGTVVFKPGD TQKEIRVGII 490 500 510 520 530 540 DDDIFEEDEN FLVHLSNVKV SSEASEDGIL EANHVSTLAC LGSPSTATVT IFDDDHAGIF 550 560 570 580 590 600 TFEEPVTHVS ESIGIMEVKV LRTSGARGNV IVPYKTIEGT ARGGGEDFED TCGELEFQND 610 620 630 640 650 660 EIVKIITIRI FDREEYEKEC SFSLVLEEPK WIRRGMKGGF TITGKYLFGQ PVFRKVHARE 670 680 690 700 710 720 HPILSTVITI ADEYDDKQPL TSKEEEERRI AEMGRPILGE HTKLEVIIEE SYEFKSTVDK 730 740 750 760 770 780 LIKKTNLALV VGTNSWREQF IEAITVSAGE DDDDDECGEE KLPSCFDYVM HFLTVFWKVL 790 800 810 820 830 840 FAFVPPTEYW NGWACFIVSI LMIGLLTAFI GDLASHFGCT IGLKDSVTAV VFVALGTSVP 850 860 870 880 890 900 DTFASKVAAT QDQYADASIG NVTGSNAVNV FLGIGVAWSI AAIYHAANGE QFKVSPGTLA 910 920 930 940 950 960 FSVTLFTIFA FINVGVLLYR RRPEIGGELG GPRTAKLLTS CLFVLLWLLY IFFSSLEAYC HIKGF
	Isoform 5 [UniParc]. Checksum: D965406E3FF9EC08	FASTA	968	107,938
	10 20 30 40 50 60 MYNMRRLSLS PTFSMGFHLL VTVSLLFSHV DHVIAETEME GEGNETGECT GSYYCKKGVI 70 80 90 100 110 120 LPIWEPQDPS FGDKIARATV YFVAMVYMFL GVSIIADRFM SSIEVITSQE KEITIKKPNG 130 140 150 160 170 180 ETTKTTVRIW NETVSNLTLM ALGSSAPEIL LSVIEVCGHN FTAGDLGPST IVGSAAFNMF 190 200 210 220 230 240 IIIALCVYVV PDGETRKIKH LRVFFVTAAW SIFAYTWLYI ILSVISPGVV EVWEGLLTFF 250 260 270 280 290 300 FFPICVVFAW VADRRLLFYK YVYKRYRAGK QRGMIIEHEG DRPSSKTEIE MDGKVVNSHV 310 320 330 340 350 360 ENFLDGALVL EVDERDQDDE EARREMARIL KELKQKHPDK EIEQLIELAN YQVLSQQQKS 370 380 390 400 410 420 RAFYRIQATR LMTGAGNILK RHAADQARKA VSMHEVNTEV TENDPVSKIF FEQGTYQCLE 430 440 450 460 470 480 NCGTVALTII RRGGDLTNTV FVDFRTEDGT ANAGSDYEFT EGTVVFKPGD TQKEIRVGII 490 500 510 520 530 540 DDDIFEEDEN FLVHLSNVKV SSEASEDGIL EANHVSTLAC LGSPSTATVT IFDDDHAGIF 550 560 570 580 590 600 TFEEPVTHVS ESIGIMEVKV LRTSGARGNV IVPYKTIEGT ARGGGEDFED TCGELEFQND 610 620 630 640 650 660 EIVKTISVKV IDDEEYEKNK TFFLEIGEPR LVEMSEKKAL LLNELGGFTI TGQPVFRKVH 670 680 690 700 710 720 AREHPILSTV ITIADEYDDK QPLTSKEEEE RRIAEMGRPI LGEHTKLEVI IEESYEFKST 730 740 750 760 770 780 VDKLIKKTNL ALVVGTNSWR EQFIEAITVS AGEDDDDDEC GEEKLPSCFD YVMHFLTVFW 790 800 810 820 830 840 KVLFAFVPPT EYWNGWACFI VSILMIGLLT AFIGDLASHF GCTIGLKDSV TAVVFVALGT 850 860 870 880 890 900 SVPDTFASKV AATQDQYADA SIGNVTGSNA VNVFLGIGVA WSIAAIYHAA NGEQFKVSPG 910 920 930 940 950 960 TLAFSVTLFT IFAFINVGVL LYRRRPEIGG ELGGPRTAKL LTSCLFVLLW LLYIFFSSLE AYCHIKGF

References

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[1]	"Molecular cloning and characterization of the human cardiac Na+/Ca2+ exchanger cDNA." Komuro I., Wenninger K.E., Philipson K.D., Izumo S. Proc. Natl. Acad. Sci. U.S.A. 89:4769-4773(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Heart.
[2]	"NCX1 Na/Ca exchanger splice variants in pancreatic islet cells." Van Eylen F., Bollen A., Herchuelz A. J. Endocrinol. 168:517-526(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 7).
[3]	"Na+/Ca2+ exchanger isoforms in cultured human retinal pigment epithelium." Mangini N.J., Chen W., Wang Q., Kennedy B.G. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 7).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Placenta.
[5]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"Na+/Ca2+ exchanger isoforms of rat odontoblasts and osteoblasts." Lundquist P., Lundgren T., Gritli-Linde A., Linde A. Calcif. Tissue Int. 67:60-67(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 459-681 (ISOFORM 10).
[8]	"Homo sapiens protein coding cDNA." Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 558-973 (ISOFORM 5). Tissue: Brain.
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 594-973 (ISOFORM 5).
[10]	"Functional expression of human renal Na+/Ca2+ exchanger in insect cells." Loo T.W., Clarke D.M. Am. J. Physiol. 267:F70-F74(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 600-684 (ISOFORM 3). Tissue: Embryonic kidney.
[11]	"Expression of the Na-Ca exchanger in diverse tissues: a study using the cloned human cardiac Na-Ca exchanger." Kofuji P., Hadley R.W., Kieval R.S., Lederer W.J., Schulze D.H. Am. J. Physiol. 263:C1241-C1249(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[12]	"The topology of the cardiac Na(+)/Ca(2)(+) exchanger, NCX1." Ren X., Philipson K.D. J. Mol. Cell. Cardiol. 57:68-71(2013) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M91368 mRNA. Translation: AAA35702.1. AF108388 mRNA. Translation: AAF08987.1. AF108389 mRNA. Translation: AAF08988.1. AF128524 mRNA. Translation: AAD26362.1. AK291696 mRNA. Translation: BAF84385.1. AC007254 Genomic DNA. Translation: AAF19235.1. AC007281 Genomic DNA. Translation: AAF19237.1. AC007377 Genomic DNA. Translation: AAX81985.1. CH471053 Genomic DNA. Translation: EAX00331.1. CH471053 Genomic DNA. Translation: EAX00332.1. CH471053 Genomic DNA. Translation: EAX00333.1. CH471053 Genomic DNA. Translation: EAX00334.1. CH471053 Genomic DNA. Translation: EAX00335.1. CH471053 Genomic DNA. Translation: EAX00336.1. AF115505 mRNA. Translation: AAD17213.1. AB209075 mRNA. Translation: BAD92312.1. BC098308 mRNA. Translation: AAH98308.1. Different initiation.
CCDS	CCDS1806.1. [P32418-1] CCDS46264.1. [P32418-2] CCDS46265.1. [P32418-5] CCDS59430.1. [P32418-4]
PIR	S32815.
RefSeq	NP_001106271.1. NM_001112800.1. [P32418-5] NP_001106272.1. NM_001112801.1. [P32418-3] NP_001106273.1. NM_001112802.1. [P32418-2] NP_001239553.1. NM_001252624.1. [P32418-4] NP_066920.1. NM_021097.2. [P32418-1] XP_005264571.1. XM_005264514.1. [P32418-2] XP_006712144.1. XM_006712081.1. [P32418-1] XP_006712145.1. XM_006712082.1. [P32418-1] XP_006712146.1. XM_006712083.1. [P32418-1] XP_006712147.1. XM_006712084.1. [P32418-1] XP_006712148.1. XM_006712085.1. [P32418-1]
UniGene	Hs.31961. Hs.468274. Hs.744988.
3D structure databases
ProteinModelPortal	P32418.
SMR	P32418. Positions 406-727.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	112436. 1 interaction.
DIP	DIP-48356N.
IntAct	P32418. 1 interaction.
Chemistry
ChEMBL	CHEMBL4076.
DrugBank	DB00132. Alpha-Linolenic Acid. DB00159. Icosapent.
Protein family/group databases
TCDB	2.A.19.3.4. the ca(2+):cation antiporter (caca) family.
PTM databases
PhosphoSite	P32418.
Polymorphism databases
DMDM	12644210.
Proteomic databases
PaxDb	P32418.
PRIDE	P32418.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000332839; ENSP00000332931; ENSG00000183023. [P32418-1] ENST00000402441; ENSP00000385188; ENSG00000183023. [P32418-2] ENST00000403092; ENSP00000384763; ENSG00000183023. [P32418-1] ENST00000405269; ENSP00000385535; ENSG00000183023. [P32418-2] ENST00000405901; ENSP00000385678; ENSG00000183023. [P32418-5] ENST00000406391; ENSP00000385811; ENSG00000183023. [P32418-2] ENST00000406785; ENSP00000383886; ENSG00000183023. [P32418-2] ENST00000408028; ENSP00000384908; ENSG00000183023. [P32418-4] ENST00000542756; ENSP00000440727; ENSG00000183023. [P32418-5]
GeneID	6546.
KEGG	hsa:6546.
UCSC	uc002rrx.3. human. [P32418-1] uc002rrz.3. human. [P32418-3] uc002rsa.3. human. [P32418-2] uc002rsb.2. human. [P32418-4]
Organism-specific databases
CTD	6546.
GeneCards	GC02M040339.
HGNC	HGNC:11068. SLC8A1.
HPA	CAB022694.
MIM	182305. gene.
neXtProt	NX_P32418.
PharmGKB	PA314.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0530.
HOGENOM	HOG000266971.
HOVERGEN	HBG006441.
InParanoid	P32418.
KO	K05849.
OMA	DDEECGE.
OrthoDB	EOG7S4X56.
PhylomeDB	P32418.
TreeFam	TF314308.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P32418.
Bgee	P32418.
CleanEx	HS_SLC8A1.
Genevestigator	P32418.
Family and domain databases
InterPro	IPR003644. Calx_beta. IPR001623. DnaJ_domain. IPR004836. Na_Ca_Ex. IPR002987. NaCa_exhngr1. IPR004837. NaCa_Exmemb. [Graphical view]
Pfam	PF03160. Calx-beta. 2 hits. PF01699. Na_Ca_ex. 2 hits. [Graphical view]
PRINTS	PR01259. NACAEXCHNGR. PR01260. NACAEXCHNGR1.
SMART	SM00237. Calx_beta. 2 hits. [Graphical view]
TIGRFAMs	TIGR00845. caca. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	SLC8A1. human.
GenomeRNAi	6546.
NextBio	25469.
PRO	P32418.
SOURCE	Search...

Entry information

Entry name

NAC1_HUMAN

Accession

Primary (citable) accession number: P32418
Secondary accession number(s): A8K6N1 , D6W595, O95849, Q4QQG6, Q587I6, Q59GN4, Q9UBL8, Q9UD55, Q9UDN1, Q9UDN2, Q9UKX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	December 1, 2000
Last modified:	July 9, 2014
This is version 145 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.