P32413 (BR2E_RANES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Brevinin-2E
Organism	Rana esculenta (Edible frog) (Pelophylax esculentus)
Taxonomic identifier	8401 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Neobatrachia › Ranoidea › Ranidae › Raninae › Rana › Pelophylax

Protein attributes

Sequence length	33 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Shows antibacterial activity against representative Gram-negative and Gram-positive bacterial species, and hemolytic activity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the skin glands.
Sequence similarities	Belongs to the frog skin active peptide (FSAP) family. Brevinin subfamily.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis
Cellular component	Secreted
Molecular function	Amphibian defense peptide Antibiotic Antimicrobial
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 33	33	Brevinin-2E		PRO_0000044642
Amino acid modifications
	Disulfide bond	27 ↔ 33		Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32413 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 99140BC640ABB0EE	FASTA	33	3,364
	10 20 30 GIMDTLKNLA KTAGKGALQS LLNKASCKLS GQC

References

[1]	"Novel antimicrobial peptides from skin secretion of the European frog Rana esculenta." Simmaco M., Mignogna G., Barra D., Bossa F. FEBS Lett. 324:159-161(1993) [PubMed: 8508915] [Abstract] Cited for: PROTEIN SEQUENCE, DISULFIDE BOND. Tissue: Skin secretion.

Cross-references

Sequence databases
PIR	S33730.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG107535.
Family and domain databases
InterPro	IPR012521. Antimicrobial_frog_2. [Graphical view]
Pfam	PF08023. Antimicrobial_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BR2E_RANES

Accession

Primary (citable) accession number: P32413

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program