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P32411

- RL44E_HALMA

UniProt

P32411 - RL44E_HALMA

Protein

50S ribosomal protein L44e

Gene

rpl44e

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds to the 23S rRNA. Binds deacetylated tRNA in the E site; when the tRNA binds a stretch of 7 amino acids are displaced to allow binding.

    Cofactori

    Binds 1 zinc ion per subunit.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi11 – 111ZincCurated
    Metal bindingi14 – 141ZincCurated
    Metal bindingi71 – 711ZincCurated
    Metal bindingi74 – 741ZincCurated

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri11 – 7464C4-typeCuratedAdd
    BLAST

    GO - Molecular functioni

    1. large ribosomal subunit rRNA binding Source: UniProtKB-HAMAP
    2. structural constituent of ribosome Source: InterPro
    3. tRNA binding Source: UniProtKB-KW
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    Metal-binding, RNA-binding, rRNA-binding, tRNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciHMAR272569:GJDH-3155-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L44eUniRule annotation
    Alternative name(s):
    HLA
    La
    Gene namesi
    Name:rpl44eUniRule annotation
    Ordered Locus Names:rrnAC3514
    OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
    Taxonomic identifieri272569 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloarcula
    ProteomesiUP000001169: Chromosome I

    Subcellular locationi

    GO - Cellular componenti

    1. ribosome Source: UniProtKB-KW

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 929250S ribosomal protein L44ePRO_0000149150Add
    BLAST

    Interactioni

    Subunit structurei

    Part of the 50S ribosomal subunit. Interacts weakly with protein L15e. Binds the E site tRNA.4 PublicationsUniRule annotation

    Protein-protein interaction databases

    STRINGi272569.rrnAC3514.

    Structurei

    Secondary structure

    1
    92
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 1110
    Turni12 – 154
    Beta strandi16 – 249
    Beta strandi32 – 343
    Helixi35 – 439
    Beta strandi45 – 473
    Helixi50 – 534
    Beta strandi61 – 633
    Beta strandi67 – 715
    Turni72 – 743
    Beta strandi77 – 793
    Beta strandi89 – 913

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FFKX-ray2.40Z1-92[»]
    1JJ2X-ray2.4021-92[»]
    1K73X-ray3.0141-92[»]
    1K8AX-ray3.0041-92[»]
    1K9MX-ray3.0041-92[»]
    1KC8X-ray3.0141-92[»]
    1KD1X-ray3.0041-92[»]
    1KQSX-ray3.1021-92[»]
    1M1KX-ray3.2041-92[»]
    1M90X-ray2.8041-92[»]
    1N8RX-ray3.0041-92[»]
    1NJIX-ray3.0041-92[»]
    1Q7YX-ray3.2041-92[»]
    1Q81X-ray2.9541-92[»]
    1Q82X-ray2.9841-92[»]
    1Q86X-ray3.0041-92[»]
    1QVFX-ray3.1021-92[»]
    1QVGX-ray2.9021-92[»]
    1S72X-ray2.4031-92[»]
    1VQ4X-ray2.7031-92[»]
    1VQ5X-ray2.6031-92[»]
    1VQ6X-ray2.7031-92[»]
    1VQ7X-ray2.5031-92[»]
    1VQ8X-ray2.2031-92[»]
    1VQ9X-ray2.4031-92[»]
    1VQKX-ray2.3031-92[»]
    1VQLX-ray2.3031-92[»]
    1VQMX-ray2.3031-92[»]
    1VQNX-ray2.4031-92[»]
    1VQOX-ray2.2031-92[»]
    1VQPX-ray2.2531-92[»]
    1W2BX-ray3.5021-92[»]
    1YHQX-ray2.4031-92[»]
    1YI2X-ray2.6531-92[»]
    1YIJX-ray2.6031-92[»]
    1YITX-ray2.8031-92[»]
    1YJ9X-ray2.9031-92[»]
    1YJNX-ray3.0031-92[»]
    1YJWX-ray2.9031-92[»]
    2OTJX-ray2.9031-92[»]
    2OTLX-ray2.7031-92[»]
    2QA4X-ray3.0031-92[»]
    2QEXX-ray2.9031-92[»]
    3CC2X-ray2.4031-92[»]
    3CC4X-ray2.7031-92[»]
    3CC7X-ray2.7031-92[»]
    3CCEX-ray2.7531-92[»]
    3CCJX-ray2.7031-92[»]
    3CCLX-ray2.9031-92[»]
    3CCMX-ray2.5531-92[»]
    3CCQX-ray2.9031-92[»]
    3CCRX-ray3.0031-92[»]
    3CCSX-ray2.9531-92[»]
    3CCUX-ray2.8031-92[»]
    3CCVX-ray2.9031-92[»]
    3CD6X-ray2.7531-92[»]
    3CMAX-ray2.8031-92[»]
    3CMEX-ray2.9531-92[»]
    3CPWX-ray2.7021-92[»]
    3CXCX-ray3.0021-92[»]
    3G4SX-ray3.2031-92[»]
    3G6EX-ray2.7031-92[»]
    3G71X-ray2.8531-92[»]
    3I55X-ray3.1131-92[»]
    3I56X-ray2.9031-92[»]
    3OW2X-ray2.7021-92[»]
    4ADXelectron microscopy6.6031-92[»]
    4HUBX-ray2.4031-92[»]
    ProteinModelPortaliP32411.
    SMRiP32411. Positions 1-92.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32411.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L44e family.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri11 – 7464C4-typeCuratedAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1631.
    HOGENOMiHOG000224988.
    KOiK02929.
    OMAiYRGYPRP.

    Family and domain databases

    Gene3Di3.10.450.80. 1 hit.
    HAMAPiMF_01476. Ribosomal_L44e.
    InterProiIPR000552. Ribosomal_L44e.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view]
    PANTHERiPTHR10369. PTHR10369. 1 hit.
    PfamiPF00935. Ribosomal_L44. 1 hit.
    [Graphical view]
    ProDomiPD002841. Ribosomal_L44e. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF57829. SSF57829. 1 hit.
    PROSITEiPS01172. RIBOSOMAL_L44E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32411-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQMPRRFNTY CPHCNEHQEH EVEKVRSGRQ TGMKWIDRQR ERNSGIGNDG   50
    KFSKVPGGDK PTKKTDLKYR CGECGKAHLR EGWRAGRLEF QE 92
    Length:92
    Mass (Da):10,791
    Last modified:October 1, 1993 - v1
    Checksum:i14197AA6AE7AC07A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY596297 Genomic DNA. Translation: AAV48183.1.
    PIRiS33790.
    RefSeqiYP_137889.1. NC_006396.1.

    Genome annotation databases

    EnsemblBacteriaiAAV48183; AAV48183; rrnAC3514.
    GeneIDi3129532.
    KEGGihma:rrnAC3514.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY596297 Genomic DNA. Translation: AAV48183.1 .
    PIRi S33790.
    RefSeqi YP_137889.1. NC_006396.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FFK X-ray 2.40 Z 1-92 [» ]
    1JJ2 X-ray 2.40 2 1-92 [» ]
    1K73 X-ray 3.01 4 1-92 [» ]
    1K8A X-ray 3.00 4 1-92 [» ]
    1K9M X-ray 3.00 4 1-92 [» ]
    1KC8 X-ray 3.01 4 1-92 [» ]
    1KD1 X-ray 3.00 4 1-92 [» ]
    1KQS X-ray 3.10 2 1-92 [» ]
    1M1K X-ray 3.20 4 1-92 [» ]
    1M90 X-ray 2.80 4 1-92 [» ]
    1N8R X-ray 3.00 4 1-92 [» ]
    1NJI X-ray 3.00 4 1-92 [» ]
    1Q7Y X-ray 3.20 4 1-92 [» ]
    1Q81 X-ray 2.95 4 1-92 [» ]
    1Q82 X-ray 2.98 4 1-92 [» ]
    1Q86 X-ray 3.00 4 1-92 [» ]
    1QVF X-ray 3.10 2 1-92 [» ]
    1QVG X-ray 2.90 2 1-92 [» ]
    1S72 X-ray 2.40 3 1-92 [» ]
    1VQ4 X-ray 2.70 3 1-92 [» ]
    1VQ5 X-ray 2.60 3 1-92 [» ]
    1VQ6 X-ray 2.70 3 1-92 [» ]
    1VQ7 X-ray 2.50 3 1-92 [» ]
    1VQ8 X-ray 2.20 3 1-92 [» ]
    1VQ9 X-ray 2.40 3 1-92 [» ]
    1VQK X-ray 2.30 3 1-92 [» ]
    1VQL X-ray 2.30 3 1-92 [» ]
    1VQM X-ray 2.30 3 1-92 [» ]
    1VQN X-ray 2.40 3 1-92 [» ]
    1VQO X-ray 2.20 3 1-92 [» ]
    1VQP X-ray 2.25 3 1-92 [» ]
    1W2B X-ray 3.50 2 1-92 [» ]
    1YHQ X-ray 2.40 3 1-92 [» ]
    1YI2 X-ray 2.65 3 1-92 [» ]
    1YIJ X-ray 2.60 3 1-92 [» ]
    1YIT X-ray 2.80 3 1-92 [» ]
    1YJ9 X-ray 2.90 3 1-92 [» ]
    1YJN X-ray 3.00 3 1-92 [» ]
    1YJW X-ray 2.90 3 1-92 [» ]
    2OTJ X-ray 2.90 3 1-92 [» ]
    2OTL X-ray 2.70 3 1-92 [» ]
    2QA4 X-ray 3.00 3 1-92 [» ]
    2QEX X-ray 2.90 3 1-92 [» ]
    3CC2 X-ray 2.40 3 1-92 [» ]
    3CC4 X-ray 2.70 3 1-92 [» ]
    3CC7 X-ray 2.70 3 1-92 [» ]
    3CCE X-ray 2.75 3 1-92 [» ]
    3CCJ X-ray 2.70 3 1-92 [» ]
    3CCL X-ray 2.90 3 1-92 [» ]
    3CCM X-ray 2.55 3 1-92 [» ]
    3CCQ X-ray 2.90 3 1-92 [» ]
    3CCR X-ray 3.00 3 1-92 [» ]
    3CCS X-ray 2.95 3 1-92 [» ]
    3CCU X-ray 2.80 3 1-92 [» ]
    3CCV X-ray 2.90 3 1-92 [» ]
    3CD6 X-ray 2.75 3 1-92 [» ]
    3CMA X-ray 2.80 3 1-92 [» ]
    3CME X-ray 2.95 3 1-92 [» ]
    3CPW X-ray 2.70 2 1-92 [» ]
    3CXC X-ray 3.00 2 1-92 [» ]
    3G4S X-ray 3.20 3 1-92 [» ]
    3G6E X-ray 2.70 3 1-92 [» ]
    3G71 X-ray 2.85 3 1-92 [» ]
    3I55 X-ray 3.11 3 1-92 [» ]
    3I56 X-ray 2.90 3 1-92 [» ]
    3OW2 X-ray 2.70 2 1-92 [» ]
    4ADX electron microscopy 6.60 3 1-92 [» ]
    4HUB X-ray 2.40 3 1-92 [» ]
    ProteinModelPortali P32411.
    SMRi P32411. Positions 1-92.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272569.rrnAC3514.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV48183 ; AAV48183 ; rrnAC3514 .
    GeneIDi 3129532.
    KEGGi hma:rrnAC3514.

    Phylogenomic databases

    eggNOGi COG1631.
    HOGENOMi HOG000224988.
    KOi K02929.
    OMAi YRGYPRP.

    Enzyme and pathway databases

    BioCyci HMAR272569:GJDH-3155-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P32411.

    Family and domain databases

    Gene3Di 3.10.450.80. 1 hit.
    HAMAPi MF_01476. Ribosomal_L44e.
    InterProi IPR000552. Ribosomal_L44e.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view ]
    PANTHERi PTHR10369. PTHR10369. 1 hit.
    Pfami PF00935. Ribosomal_L44. 1 hit.
    [Graphical view ]
    ProDomi PD002841. Ribosomal_L44e. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF57829. SSF57829. 1 hit.
    PROSITEi PS01172. RIBOSOMAL_L44E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "HL35e and HLA: primary structure of two very basic and cysteine-rich ribosomal proteins from Haloarcula marismortui."
      Bergmann U., Wittmann-Liebold B.
      Biochim. Biophys. Acta 1173:195-200(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, SUBUNIT.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    3. "The complete atomic structure of the large ribosomal subunit at 2.4 A resolution."
      Ban N., Nissen P., Hansen J., Moore P.B., Steitz T.A.
      Science 289:905-920(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT, SUBUNIT, RRNA-BINDING, PROBABLE COFACTOR.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    4. "The structural basis of ribosome activity in peptide bond synthesis."
      Nissen P., Hansen J., Ban N., Moore P.B., Steitz T.A.
      Science 289:920-930(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    5. "A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits."
      Schmeing T.M., Seila A.C., Hansen J.L., Freeborn B., Soukup J.K., Scaringe S.A., Strobel S.A., Moore P.B., Steitz T.A.
      Nat. Struct. Biol. 9:225-230(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    6. "The kink-turn: a new RNA secondary structure motif."
      Klein D.J., Schmeing T.M., Moore P.B., Steitz T.A.
      EMBO J. 20:4214-4221(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    7. "The structures of four macrolide antibiotics bound to the large ribosomal subunit."
      Hansen J.L., Ippolito J.A., Ban N., Nissen P., Moore P.B., Steitz T.A.
      Mol. Cell 10:117-128(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FOUR MACROLIDE ANTIBIOTICS.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    8. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF THE 50S SUBUNIT.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    9. "Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit."
      Hansen J.L., Moore P.B., Steitz T.A.
      J. Mol. Biol. 330:1061-1075(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF THE 50S SUBUNIT IN COMPLEX WITH FIVE ANTIBIOTICS AT THE PEPTIDYL TRANSFERASE CENTER.
      Strain: ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809.
    10. "Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit."
      Schmeing T.M., Moore P.B., Steitz T.A.
      RNA 9:1345-1352(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF THE 50S SUBUNIT WITH TWO DIFFERENT E SITE SUBSTRATES, RRNA-BINDING, TRNA-BINDING.
    11. "Revisiting the Haloarcula marismortui 50S ribosomal subunit model."
      Gabdulkhakov A., Nikonov S., Garber M.
      Acta Crystallogr. D 69:997-1004(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF THE 50S SUBUNIT.

    Entry informationi

    Entry nameiRL44E_HALMA
    AccessioniPrimary (citable) accession number: P32411
    Secondary accession number(s): Q5UX20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3