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P32400

- CSH_ARTSP

UniProt

P32400 - CSH_ARTSP

Protein

N-carbamoylsarcosine amidase

Gene
N/A
Organism
Arthrobacter sp.
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (16 Oct 2013)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    N-carbamoylsarcosine + H2O = sarcosine + CO2 + NH3.

    Cofactori

    Binds 1 sulfate ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei177 – 1771Nucleophile

    GO - Molecular functioni

    1. N-carbamoylsarcosine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. creatinine catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-11021.
    UniPathwayiUPA00274; UER00397.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-carbamoylsarcosine amidase (EC:3.5.1.59)
    Alternative name(s):
    N-carbamoylsarcosine amidohydrolase
    Short name:
    CSHase
    OrganismiArthrobacter sp.
    Taxonomic identifieri1667 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 264264N-carbamoylsarcosine amidasePRO_0000079397Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    Secondary structure

    1
    264
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 3325
    Beta strandi43 – 519
    Helixi54 – 574
    Beta strandi58 – 614
    Helixi68 – 8417
    Beta strandi89 – 946
    Helixi109 – 1135
    Helixi117 – 1193
    Helixi125 – 1273
    Helixi131 – 1333
    Beta strandi140 – 15011
    Helixi155 – 1617
    Beta strandi166 – 1727
    Turni174 – 1763
    Helixi177 – 18812
    Beta strandi191 – 1955
    Helixi196 – 1983
    Beta strandi202 – 2054
    Helixi206 – 21712
    Beta strandi220 – 2223
    Helixi224 – 23310
    Helixi237 – 2393

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NBAX-ray2.00A/B/C/D1-264[»]
    ProteinModelPortaliP32400.
    SMRiP32400. Positions 7-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP32400.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.50.850. 1 hit.
    InterProiIPR000868. Isochorismatase-like.
    [Graphical view]
    PfamiPF00857. Isochorismatase. 1 hit.
    [Graphical view]
    SUPFAMiSSF52499. SSF52499. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P32400-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTETSGTFND IEARLAAVLE EAFEAGTSIY NERGFKRRIG YGNRPAVIHI    50
    DLANAWTQPG HPFSCPGMET IIPNVQRINE AARAKGVPVF YTTNVYRNRD 100
    ASSGTNDMGL WYSKIPTETL PADSYWAQID DRIAPADGEV VIEKNRASAF 150
    PGTNLELFLT SNRIDTLIVT GATAAGCVRH TVEDAIAKGF RPIIPRETIG 200
    DRVPGVVQWN LYDIDNKFGD VESTDSVVQY LDALPQFEDT VPKTLSDPQP 250
    EVEAPADPVF AEQH 264
    Length:264
    Mass (Da):29,057
    Last modified:October 1, 1993 - v1
    Checksum:i8A213B555EA5DCDC
    GO

    Sequence databases

    PIRiS28969.

    Cross-referencesi

    Sequence databases

    PIRi S28969.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NBA X-ray 2.00 A/B/C/D 1-264 [» ]
    ProteinModelPortali P32400.
    SMRi P32400. Positions 7-259.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00274 ; UER00397 .
    BioCyci MetaCyc:MONOMER-11021.

    Miscellaneous databases

    EvolutionaryTracei P32400.

    Family and domain databases

    Gene3Di 3.40.50.850. 1 hit.
    InterProi IPR000868. Isochorismatase-like.
    [Graphical view ]
    Pfami PF00857. Isochorismatase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52499. SSF52499. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Crystal structure analysis, refinement and enzymatic reaction mechanism of N-carbamoylsarcosine amidohydrolase from Arthrobacter sp. at 2.0-A resolution."
      Romao M.J., Turk D., Gomis-Rueth F.-X., Huber R.
      J. Mol. Biol. 226:1111-1130(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SEQUENCE REVISION TO 184 AND 232.
    2. "Crystallographic and fluorescence studies of ligand binding to N-carbamoylsarcosine amidohydrolase from Arthrobacter sp."
      Zajc A., Romao M.J., Turk D., Huber R.
      J. Mol. Biol. 263:269-283(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.28 ANGSTROMS) OF COMPLEX WITH INHIBITORS.

    Entry informationi

    Entry nameiCSH_ARTSP
    AccessioniPrimary (citable) accession number: P32400
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 16, 2013
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3