Reviewed, UniProtKB/Swiss-Prot P32397 (PPOX_BACSU)

Names and origin

Protein names

Recommended name:
    Protoporphyrinogen oxidase
      Short name=PPO
    EC=1.3.3.4

Gene names

Name:	hemY
Synonyms:	hemG
Ordered Locus Names:	BSU10140

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. Also oxidizes the pathway intermediate coproporphyrinogen-III.
Catalytic activity	Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.
Cofactor	Binds 1 FAD per dimer By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the protoporphyrinogen oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro protoporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Protoporphyrinogen oxidase		PRO_0000135263
Regions
	Nucleotide binding	12 – 17	6	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P32397-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 95CC4E5847686D2E	FASTA	470	51,203
	10 20 30 40 50 60 MSDGKKHVVI IGGGITGLAA AFYMEKEIKE KNLPLELTLV EASPRVGGKI QTVKKDGYII 70 80 90 100 110 120 ERGPDSFLER KKSAPQLVKD LGLEHLLVNN ATGQSYVLVN RTLHPMPKGA VMGIPTKIAP 130 140 150 160 170 180 FVSTGLFSLS GKARAAMDFI LPASKTKDDQ SLGEFFRRRV GDEVVENLIE PLLSGIYAGD 190 200 210 220 230 240 IDKLSLMSTF PQFYQTEQKH RSLILGMKKT RPQGSGQQLT AKKQGQFQTL STGLQTLVEE 250 260 270 280 290 300 IEKQLKLTKV YKGTKVTKLS HSGSCYSLEL DNGVTLDADS VIVTAPHKAA AGMLSELPAI 310 320 330 340 350 360 SHLKNMHSTS VANVALGFPE GSVQMEHEGT GFVISRNSDF AITACTWTNK KWPHAAPEGK 370 380 390 400 410 420 TLLRAYVGKA GDESIVDLSD NDIINIVLED LKKVMNINGE PEMTCVTRWH ESMPQYHVGH 430 440 450 460 470 KQRIKELREA LASAYPGVYM TGASFEGVGI PDCIDQGKAA VSDALTYLFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes." Hansson M., Hederstedt L. J. Bacteriol. 174:8081-8093(1992) [PubMed: 1459957] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene." Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S. Microbiology 144:859-875(1998) [PubMed: 9579061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Expression of a cloned protoporphyrinogen oxidase." Dailey T.A., Meissner P., Dailey H.A. J. Biol. Chem. 269:813-815(1994) [PubMed: 8288631] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M97208 Genomic DNA. Translation: AAA22519.1. Y14083 Genomic DNA. Translation: CAA74520.1. AL009126 Genomic DNA. Translation: CAB12854.1.
PIR	D47045.
RefSeq	NP_388895.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	936311.
GenomeReviews	Gene locus BSU10140 in contig AL009126_GR.
KEGG	bsu:BSU10140.
NMPDR	fig|224308.1.peg.1014.
Organism-specific databases
SubtiList	BG10431. hemY. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P32397.
OMA	P32397. EGSERLG.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1014-MON.
BRENDA	1.3.3.4. 150.
Family and domain databases
InterPro	IPR002937. Amino_oxidase. IPR004572. Protoporphyrinogen_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00562. proto_IX_ox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPOX_BACSU

Accession

Primary (citable) accession number: P32397

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)