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Protein

Uroporphyrinogen decarboxylase

Gene

hemE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.1 Publication

Catalytic activityi

Uroporphyrinogen III = coproporphyrinogen + 4 CO2.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (hemB)
  2. Porphobilinogen deaminase (hemC)
  3. Uroporphyrinogen-III synthase (hemD)
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48SubstrateBy similarity1
Binding sitei78SubstrateBy similarity1
Sitei78Transition state stabilizerBy similarity1
Binding sitei154SubstrateBy similarity1
Binding sitei209SubstrateBy similarity1
Binding sitei322SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU10120-MONOMER.
MetaCyc:BSU10120-MONOMER.
UniPathwayiUPA00251; UER00321.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen decarboxylase (EC:4.1.1.37)
Short name:
UPD
Short name:
URO-D
Gene namesi
Name:hemE
Ordered Locus Names:BSU10120
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001875841 – 353Uroporphyrinogen decarboxylaseAdd BLAST353

Proteomic databases

PaxDbiP32395.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005626.

Structurei

Secondary structure

1353
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 15Combined sources6
Beta strandi30 – 32Combined sources3
Helixi36 – 52Combined sources17
Helixi54 – 68Combined sources15
Turni79 – 82Combined sources4
Helixi83 – 86Combined sources4
Beta strandi94 – 96Combined sources3
Beta strandi99 – 102Combined sources4
Helixi107 – 111Combined sources5
Helixi118 – 121Combined sources4
Helixi123 – 135Combined sources13
Beta strandi141 – 146Combined sources6
Helixi148 – 157Combined sources10
Helixi165 – 173Combined sources9
Helixi175 – 198Combined sources24
Beta strandi202 – 207Combined sources6
Helixi211 – 213Combined sources3
Helixi216 – 222Combined sources7
Helixi224 – 234Combined sources11
Helixi235 – 237Combined sources3
Beta strandi241 – 244Combined sources4
Helixi249 – 251Combined sources3
Helixi252 – 256Combined sources5
Beta strandi261 – 264Combined sources4
Helixi271 – 276Combined sources6
Beta strandi281 – 284Combined sources4
Helixi289 – 293Combined sources5
Helixi296 – 310Combined sources15
Beta strandi316 – 318Combined sources3
Beta strandi320 – 322Combined sources3
Helixi330 – 348Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2INFX-ray2.30A/B/C/D1-353[»]
ProteinModelPortaliP32395.
SMRiP32395.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32395.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 33Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CFZ. Bacteria.
COG0407. LUCA.
HOGENOMiHOG000253897.
InParanoidiP32395.
KOiK01599.
OMAiGSSKDFR.
PhylomeDBiP32395.

Family and domain databases

CDDicd00717. URO-D. 1 hit.
HAMAPiMF_00218. URO_D. 1 hit.
InterProiIPR006361. Uroporphyrinogen_deCO2ase_HemE.
IPR000257. Uroporphyrinogen_deCOase.
[Graphical view]
PfamiPF01208. URO-D. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01464. hemE. 1 hit.
PROSITEiPS00906. UROD_1. 1 hit.
PS00907. UROD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRETFNET FLKAARGEKA DHTPVWYMRQ AGRSQPEYRK LKEKYGLFEI
60 70 80 90 100
THQPELCAYV TRLPVEQYGV DAAILYKDIM TPLPSIGVDV EIKNGIGPVI
110 120 130 140 150
DQPIRSLADI EKLGQIDPEQ DVPYVLETIK LLVNEQLNVP LIGFSGAPFT
160 170 180 190 200
LASYMIEGGP SKNYNKTKAF MYSMPDAWNL LMSKLADMII VYVKAQIEAG
210 220 230 240 250
AKAIQIFDSW VGALNQADYR TYIKPVMNRI FSELAKENVP LIMFGVGASH
260 270 280 290 300
LAGDWHDLPL DVVGLDWRLG IDEARSKGIT KTVQGNLDPS ILLAPWEVIE
310 320 330 340 350
QKTKEILDQG MESDGFIFNL GHGVFPDVSP EVLKKLTAFV HEYSQNKKMG

QYS
Length:353
Mass (Da):39,647
Last modified:October 1, 1993 - v1
Checksum:iF499C26BBAFA337E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97208 Genomic DNA. Translation: AAA22517.1.
Y14083 Genomic DNA. Translation: CAA74518.1.
AL009126 Genomic DNA. Translation: CAB12852.1.
PIRiB47045.
RefSeqiNP_388893.1. NC_000964.3.
WP_009966929.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB12852; CAB12852; BSU10120.
GeneIDi936296.
KEGGibsu:BSU10120.
PATRICi18973720. VBIBacSub10457_1054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97208 Genomic DNA. Translation: AAA22517.1.
Y14083 Genomic DNA. Translation: CAA74518.1.
AL009126 Genomic DNA. Translation: CAB12852.1.
PIRiB47045.
RefSeqiNP_388893.1. NC_000964.3.
WP_009966929.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2INFX-ray2.30A/B/C/D1-353[»]
ProteinModelPortaliP32395.
SMRiP32395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005626.

Proteomic databases

PaxDbiP32395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12852; CAB12852; BSU10120.
GeneIDi936296.
KEGGibsu:BSU10120.
PATRICi18973720. VBIBacSub10457_1054.

Phylogenomic databases

eggNOGiENOG4105CFZ. Bacteria.
COG0407. LUCA.
HOGENOMiHOG000253897.
InParanoidiP32395.
KOiK01599.
OMAiGSSKDFR.
PhylomeDBiP32395.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00321.
BioCyciBSUB:BSU10120-MONOMER.
MetaCyc:BSU10120-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP32395.

Family and domain databases

CDDicd00717. URO-D. 1 hit.
HAMAPiMF_00218. URO_D. 1 hit.
InterProiIPR006361. Uroporphyrinogen_deCO2ase_HemE.
IPR000257. Uroporphyrinogen_deCOase.
[Graphical view]
PfamiPF01208. URO-D. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01464. hemE. 1 hit.
PROSITEiPS00906. UROD_1. 1 hit.
PS00907. UROD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCUP_BACSU
AccessioniPrimary (citable) accession number: P32395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.