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P32395 (DCUP_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uroporphyrinogen decarboxylase
Short name=UPD
Short name=URO-D
EC=4.1.1.37
Gene names
Name:hemE
Ordered Locus Names:BSU10120
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III. Ref.4

Catalytic activity

Uroporphyrinogen III = coproporphyrinogen + 4 CO2. HAMAP-Rule MF_00218

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4. HAMAP-Rule MF_00218

Subunit structure

Homodimer. Ref.4

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the uroporphyrinogen decarboxylase family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Cellular componentCytoplasm
   Molecular functionDecarboxylase
Lyase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionuroporphyrinogen decarboxylase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Uroporphyrinogen decarboxylase HAMAP-Rule MF_00218
PRO_0000187584

Regions

Region29 – 335Substrate binding By similarity

Sites

Binding site481Substrate By similarity
Binding site781Substrate By similarity
Binding site1541Substrate By similarity
Binding site2091Substrate By similarity
Binding site3221Substrate By similarity
Site781Transition state stabilizer By similarity

Secondary structure

1 ............................................................ 353
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32395 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: F499C26BBAFA337E

FASTA35339,647
        10         20         30         40         50         60 
MSKRETFNET FLKAARGEKA DHTPVWYMRQ AGRSQPEYRK LKEKYGLFEI THQPELCAYV 

        70         80         90        100        110        120 
TRLPVEQYGV DAAILYKDIM TPLPSIGVDV EIKNGIGPVI DQPIRSLADI EKLGQIDPEQ 

       130        140        150        160        170        180 
DVPYVLETIK LLVNEQLNVP LIGFSGAPFT LASYMIEGGP SKNYNKTKAF MYSMPDAWNL 

       190        200        210        220        230        240 
LMSKLADMII VYVKAQIEAG AKAIQIFDSW VGALNQADYR TYIKPVMNRI FSELAKENVP 

       250        260        270        280        290        300 
LIMFGVGASH LAGDWHDLPL DVVGLDWRLG IDEARSKGIT KTVQGNLDPS ILLAPWEVIE 

       310        320        330        340        350 
QKTKEILDQG MESDGFIFNL GHGVFPDVSP EVLKKLTAFV HEYSQNKKMG QYS

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes."
Hansson M., Hederstedt L.
J. Bacteriol. 174:8081-8093(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene."
Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S.
Microbiology 144:859-875(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Crystal structure of uroporphyrinogen decarboxylase from Bacillus subtilis."
Fan J., Liu Q., Hao Q., Teng M., Niu L.
J. Bacteriol. 189:3573-3580(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), FUNCTION, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M97208 Genomic DNA. Translation: AAA22517.1.
Y14083 Genomic DNA. Translation: CAA74518.1.
AL009126 Genomic DNA. Translation: CAB12852.1.
PIRB47045.
RefSeqNP_388893.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2INFX-ray2.30A/B/C/D1-353[»]
ProteinModelPortalP32395.
SMRP32395. Positions 6-349.
ModBaseSearch...

Protein-protein interaction databases

STRING224308.BSU10120.

Proteomic databases

PaxDbP32395.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12852; CAB12852; BSU10120.
GeneID936296.
KEGGbsu:BSU10120.
PATRIC18973720. VBIBacSub10457_1054.

Organism-specific databases

GenoListBSU10120. [Micado]

Phylogenomic databases

eggNOGCOG0407.
HOGENOMHOG000253897.
KOK01599.
OMATLATYMI.
ProtClustDBPRK00115.

Enzyme and pathway databases

BioCycBSUB:BSU10120-MONOMER.
UniPathwayUPA00251; UER00321.

Family and domain databases

HAMAPMF_00218. URO-D.
InterProIPR006361. Uroporphyrinogen_deCO2ase_HemE.
IPR000257. Uroporphyrinogen_deCOase.
[Graphical view]
PANTHERPTHR21091:SF2. PTHR21091:SF2. 1 hit.
PfamPF01208. URO-D. 1 hit.
[Graphical view]
TIGRFAMsTIGR01464. hemE. 1 hit.
PROSITEPS00906. UROD_1. 1 hit.
PS00907. UROD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP32395.

Entry information

Entry nameDCUP_BACSU
AccessionPrimary (citable) accession number: P32395
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents