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Reviewed, UniProtKB/Swiss-Prot P32390 (ARP3_SCHPO)

Last modified November 24, 2009. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Actin-related protein 3
Alternative name(s):
    Actin-like protein 3
Gene names
Name: act2
ORF Names: SPAC630.03
OrganismSchizosaccharomyces pombe (Fission yeast) [Complete proteome]
Taxonomic identifier4896 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament By similarity. May be involved in cytokinesis.

Subunit structure

Component of the Arp2/3 complex composed of arp2, act2, arc1/p41-ARC, arc2/p34-ARC, arc3/p21-ARC, arc4/p20-ARC and arc5/p16-ARC.

Subcellular location

Cytoplasmcytoskeletonactin patch.

Sequence similarities

Belongs to the actin family. ARP3 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Actin-related protein 3
PRO_0000089088

Secondary structure

..................................................... 427
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32390-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: C7909FFEE544789B

FASTA42747,373
        10         20         30         40         50         60 
MASFNVPIIM DNGTGYSKLG YAGNDAPSYV FPTVIATRSA GASSGPAVSS KPSYMASKGS 

        70         80         90        100        110        120 
GHLSSKRATE DLDFFIGNDA LKKASAGYSL DYPIRHGQIE NWDHMERFWQ QSLFKYLRCE 

       130        140        150        160        170        180 
PEDHYFLLTE PPLNPPENRE NTAEIMFESF NCAGLYIAVQ AVLALAASWT SSKVTDRSLT 

       190        200        210        220        230        240 
GTVVDSGDGV THIIPVAEGY VIGSSIKTMP LAGRDVTYFV QSLLRDRNEP DSSLKTAERI 

       250        260        270        280        290        300 
KEECCYVCPD IVKEFSRFDR EPDRYLKYAS ESITGHSTTI DVGFERFLAP EIFFNPEIAS 

       310        320        330        340        350        360 
SDFLTPLPEL VDNVVQSSPI DVRKGLYKNI VLSGGSTLFK NFGNRLQRDL KRIVDERIHR 

       370        380        390        400        410        420 
SEMLSGAKSG GVDVNVISHK RQRNAVWFGG SLLAQTPEFG SYCHTKADYE EYGASIARRY 


QIFGNSL 

« Hide

References

« Hide 'large scale' references
[1]"Identification of act2, an essential gene in the fission yeast Schizosaccharomyces pombe that encodes a protein related to actin."
Lees-Miller J.P., Henry G., Helfman D.M.
Proc. Natl. Acad. Sci. U.S.A. 89:80-83(1992) [PubMed: 1729722] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 38366 / 972.
[3]"A mutant of arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast."
Morrell J.L., Morphew M., Gould K.L.
Mol. Biol. Cell 10:4201-4215(1999) [PubMed: 10588653] [Abstract]
Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

M81068 Genomic DNA. No translation available.
CU329670 Genomic DNA. Translation: CAB52725.1.
PIRA41790.
RefSeqNP_592898.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
3DWLX-ray3.78A/B1-427[»]
ModBaseSearch...

Protein-protein interaction databases

STRINGP32390.

Genome annotation databases

GeneID2543233.
GenomeReviewsGene locus act2 in contig CU329670_GR.
KEGGspo:SPAC630.03.
NMPDRfig|4896.1.peg.2868.

Organism-specific databases

GeneDB_SpombeSPAC630.03.

Phylogenomic databases

OMARVEPEDH
OrthoDBEOG95MPNF

Gene expression databases

ArrayExpressP32390.

Family and domain databases

InterProIPR004000. Actin-like.
IPR015623. Arp3.
[Graphical view]
PANTHERPTHR11937. Actin_like. 1 hit.
PTHR11937:SF31. Arp3. 1 hit.
PfamPF00022. Actin. 1 hit.
[Graphical view]
SMARTSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameARP3_SCHPO
AccessionPrimary (citable) accession number: P32390
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents