P32388 (RM49_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein MRP49, mitochondrial

Gene names

Name:	MRP49
Ordered Locus Names:	YKL167C
ORF Names:	YKL631

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not essential in cells grown at 30 degree Celsius but is required for mitochondrial translation in cells grown at 18 degree Celsius.
Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S).
Subcellular location	Mitochondrion Ref.6 Ref.7.

Ontologies

Keywords
Cellular component	Mitochondrion
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial translation Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	mitochondrial large ribosomal subunit Inferred from direct assay Ref.1. Source: SGD
Molecular function	structural constituent of ribosome Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	54S ribosomal protein MRP49, mitochondrial		PRO_0000030583

Sequences

	Sequence		Length	Mass (Da)
	P32388 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 34176EEAC91A14CD	FASTA	137	16,021
	10 20 30 40 50 60 MSKVAQQLKF LNKISATTRL PQILVDPKKY SGLRLTFQTK NHNGHMGARV FWHNYLPTLQ 70 80 90 100 110 120 FYNPRMKFDV IRIKNEDKQK SVPCKLEILS HEGSVVETID MRNKMHEDIM KDLLDKIEHV 130 PLPENEIIRV GPQESII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function of MRP20 and MRP49, the nuclear genes for two proteins of the 54 S subunit of the yeast mitochondrial ribosome." Fearon K., Mason T.L. J. Biol. Chem. 267:5162-5170(1992) [PubMed: 1544898] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S25-S33(1994) [PubMed: 8091858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M81697 Genomic DNA. Translation: AAA34792.1. Z26878 Genomic DNA. Translation: CAA81520.1. Z28167 Genomic DNA. Translation: CAA82009.1. AY692991 Genomic DNA. Translation: AAT93010.1. BK006944 Genomic DNA. Translation: DAA08998.1.
PIR	A42105.
RefSeq	NP_012754.1. NM_001179733.1.
3D structure databases
ProteinModelPortal	P32388.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6767N.
IntAct	P32388. 4 interactions.
MINT	MINT-652794.
STRING	P32388.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL167C; YKL167C; YKL167C.
GeneID	853687.
KEGG	sce:YKL167C.
NMPDR	fig|4932.3.peg.3732.
Organism-specific databases
CYGD	YKL167c.
SGD	S000001650. MRP49.
Phylogenomic databases
eggNOG	fuNOG11015.
HOGENOM	HBG202408.
OMA	NGHMGAR.
OrthoDB	EOG45QMPX.
Gene expression databases
ArrayExpress	P32388.
Genevestigator	P32388.
GermOnline	YKL167C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007741. Ribosome/NADH_DH. [Graphical view]
Pfam	PF05047. L51_S25_CI-B8. 1 hit. [Graphical view]
SMART	SM00916. L51_S25_CI-B8. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974658.

Entry information

Entry name

RM49_YEAST

Accession

Primary (citable) accession number: P32388
Secondary accession number(s): D6VX32

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program