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Protein

54S ribosomal protein L41, mitochondrial

Gene

MRP20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.2 Publications

Miscellaneous

Present with 2200 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • mitochondrial translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29949-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L41, mitochondrial
Alternative name(s):
Mitochondrial large ribosomal subunit protein uL23m1 Publication
YmL41
Gene namesi
Name:MRP20
Synonyms:MRPL41
Ordered Locus Names:YDR405W
ORF Names:D9509.23
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR405W.
SGDiS000002813. MRP20.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 45Mitochondrion1 PublicationAdd BLAST45
ChainiPRO_000003048846 – 26354S ribosomal protein L41, mitochondrialAdd BLAST218

Proteomic databases

MaxQBiP32387.
PRIDEiP32387.

PTM databases

iPTMnetiP32387.

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S small subunit contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins. uL23m forms the wall of the exit tunnel (PubMed:2060626, PubMed:9151978, PubMed:24675956). Interacts with the C-terminus of OXA1 (PubMed:14657017).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MRPL40P365344EBI-15599,EBI-15595

Protein-protein interaction databases

BioGridi32465. 148 interactors.
DIPiDIP-6703N.
IntActiP32387. 9 interactors.
MINTiMINT-650158.
STRINGi4932.YDR405W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20P1-263[»]
5MRCelectron microscopy3.25P55-261[»]
5MREelectron microscopy3.75P55-261[»]
5MRFelectron microscopy4.97P55-261[»]
ProteinModelPortaliP32387.
SMRiP32387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000246534.
InParanoidiP32387.
KOiK02892.
OMAiLYFPKAR.
OrthoDBiEOG092C4LSY.

Family and domain databases

InterProiView protein in InterPro
IPR012678. Ribosomal_L23/L15e_core_dom.
IPR013025. Ribosomal_L25/23.
PfamiView protein in Pfam
PF00276. Ribosomal_L23. 1 hit.
SUPFAMiSSF54189. SSF54189. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32387-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRLTVGTKN MLYPLQKTLA VGSCKPEQVP IRSLASVVES SSKILDKSGS
60 70 80 90 100
DREVDINVSE KIYKWTKAGI EQGKEHFKVG GNKVYFPKAR IILLRPNAKH
110 120 130 140 150
TPYQAKFIVP KSFNKLDLRD YLYHIYGLRA MNITTQLLHG KFNRMNLQTT
160 170 180 190 200
RFREPQIKKM TIEMEEPFIW PEEPRPDENS FWDSTTPDNM EKYREERLNC
210 220 230 240 250
LGSDANKPGT AFDGVVGPYE RVAQPFIPRF LKREIDNKRE RHAAELQRAD
260
KLIALNRYIE DLH
Length:263
Mass (Da):30,566
Last modified:October 1, 1993 - v1
Checksum:iB8B1E51BAC60C673
GO

Sequence cautioni

The sequence AAA34790 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52R → Q AA sequence (PubMed:2060626).Curated1
Sequence conflicti155P → Y AA sequence (PubMed:9151978).Curated1
Sequence conflicti253I → K AA sequence (PubMed:9151978).Curated1
Sequence conflicti259I → K AA sequence (PubMed:9151978).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81696 Genomic DNA. Translation: AAA34789.1.
M81696 Genomic DNA. Translation: AAA34790.1. Different initiation.
U32274 Genomic DNA. Translation: AAB64845.1.
BK006938 Genomic DNA. Translation: DAA12247.1.
PIRiB42105.
RefSeqiNP_010693.3. NM_001180713.3.

Genome annotation databases

EnsemblFungiiYDR405W; YDR405W; YDR405W.
GeneIDi852014.
KEGGisce:YDR405W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRM41_YEAST
AccessioniPrimary (citable) accession number: P32387
Secondary accession number(s): D6VT37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 5, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names