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Protein

ATP-dependent bile acid permease

Gene

YBT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Vacuolar class C ABC transporter which regulates the translocation of phosphatidylcholine to the vacuole lumen, the release of lumenal calcium stores, and acts as a negative regulator of vacuole fusion. Exhibits ATP-dependent bile acid transport.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi729 – 7368ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1415 – 14228ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • bile acid-exporting ATPase activity Source: SGD

GO - Biological processi

  • bile acid and bile salt transport Source: SGD
  • calcium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32147-MONOMER.

Protein family/group databases

TCDBi3.A.1.208.12. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent bile acid permease
Gene namesi
Name:YBT1
Synonyms:BAT1
Ordered Locus Names:YLL048C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL048C.
SGDiS000003971. YBT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333LumenalBy similarityAdd
BLAST
Transmembranei34 – 5421Helical; Name=1PROSITE-ProRule annotationAdd
BLAST
Topological domaini55 – 7420CytoplasmicBy similarityAdd
BLAST
Transmembranei75 – 9521Helical; Name=2PROSITE-ProRule annotationAdd
BLAST
Topological domaini96 – 13338LumenalBy similarityAdd
BLAST
Transmembranei134 – 15421Helical; Name=3PROSITE-ProRule annotationAdd
BLAST
Topological domaini155 – 16612CytoplasmicBy similarityAdd
BLAST
Transmembranei167 – 18721Helical; Name=4PROSITE-ProRule annotationAdd
BLAST
Topological domaini188 – 20518LumenalBy similarityAdd
BLAST
Transmembranei206 – 22621Helical; Name=5PROSITE-ProRule annotationAdd
BLAST
Topological domaini227 – 345119CytoplasmicBy similarityAdd
BLAST
Transmembranei346 – 36621Helical; Name=6PROSITE-ProRule annotationAdd
BLAST
Topological domaini367 – 39327LumenalBy similarityAdd
BLAST
Transmembranei394 – 41421Helical; Name=7PROSITE-ProRule annotationAdd
BLAST
Topological domaini415 – 49581CytoplasmicBy similarityAdd
BLAST
Transmembranei496 – 51621Helical; Name=8PROSITE-ProRule annotationAdd
BLAST
Topological domaini517 – 5193LumenalBy similarity
Transmembranei520 – 54021Helical; Name=9PROSITE-ProRule annotationAdd
BLAST
Topological domaini541 – 60262CytoplasmicBy similarityAdd
BLAST
Transmembranei603 – 62321Helical; Name=10PROSITE-ProRule annotationAdd
BLAST
Topological domaini624 – 64421LumenalBy similarityAdd
BLAST
Transmembranei645 – 66521Helical; Name=11PROSITE-ProRule annotationAdd
BLAST
Topological domaini666 – 1053388CytoplasmicBy similarityAdd
BLAST
Transmembranei1054 – 107421Helical; Name=12PROSITE-ProRule annotationAdd
BLAST
Topological domaini1075 – 111440LumenalBy similarityAdd
BLAST
Transmembranei1115 – 113521Helical; Name=13PROSITE-ProRule annotationAdd
BLAST
Topological domaini1136 – 117843CytoplasmicBy similarityAdd
BLAST
Transmembranei1179 – 119921Helical; Name=14PROSITE-ProRule annotationAdd
BLAST
Topological domaini1200 – 12001LumenalBy similarity
Transmembranei1201 – 122121Helical; Name=15PROSITE-ProRule annotationAdd
BLAST
Topological domaini1222 – 129271CytoplasmicBy similarityAdd
BLAST
Transmembranei1293 – 131321Helical; Name=16PROSITE-ProRule annotationAdd
BLAST
Topological domaini1314 – 13152LumenalBy similarity
Transmembranei1316 – 133621Helical; Name=17PROSITE-ProRule annotationAdd
BLAST
Topological domaini1337 – 1661325CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16611661ATP-dependent bile acid permeasePRO_0000093447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence analysis
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence analysis
Modified residuei936 – 9361PhosphoserineCombined sources
Modified residuei940 – 9401PhosphoserineCombined sources
Modified residuei955 – 9551PhosphoserineCombined sources

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32386.

PTM databases

iPTMnetiP32386.

Interactioni

Protein-protein interaction databases

BioGridi31267. 40 interactions.
DIPiDIP-6474N.
IntActiP32386. 8 interactions.
MINTiMINT-603723.

Structurei

3D structure databases

ProteinModelPortaliP32386.
SMRiP32386. Positions 463-964, 1109-1634.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini354 – 662309ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini694 – 935242ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini1026 – 1345320ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1381 – 1636256ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00840000131000.
InParanoidiP32386.
OrthoDBiEOG70KGXT.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 3 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHHVLNSTRP DHRFWFYDDV TQYGRTKYLN YYTPLVLLIF TVLFITYNIW
60 70 80 90 100
KHYYYYDVLH LKQKNPIDEL LYSSTDEDEQ SPLINNNTIT TNYVDNNCTK
110 120 130 140 150
DALKNRHFSL EKLKSVKVNG EPHGTPEIVR RGFIEKSRII LEFFLVLSQV
160 170 180 190 200
IIHSFILLHY VNKNPEFTQQ GTITGLVEWC ALFIIVSLRL ANVNQNFKFI
210 220 230 240 250
NKYPGNLWSV SFINYLALFI SMILPFRSIF IHHINSPISR KYYISQISIN
260 270 280 290 300
LALFLLLFFA RIRNNFAIIY KTDSWITPSP EPVTSIAGFI CWAWLDSFVW
310 320 330 340 350
KAHKVSIKVK DIWGLMMQDY SFFVVKKFRY FVDHKVKRKR IFSLNLFFFF
360 370 380 390 400
SNYLVLQCFW AFLGSVLSFI PTVLLKRILE YVEDQSSAPS NLAWFYVTVM
410 420 430 440 450
FVGRILVAIC QAQALFFGRR VCIRMKSIII SEIYTKALRR KISTNKTKPS
460 470 480 490 500
NEDPQEINDQ KSINGDEEST SSANLGAIIN LMAIDAFKVS EICGYLHSFL
510 520 530 540 550
EAFVMTVVAL ALLYRLLGFA AIVGVLIIVA MLPLNYKLAK YIGDLQKKNL
560 570 580 590 600
AVTDNRIQKL NEAFQAIRII KYFSWEENFE KDINTIRENE LSLLLMRSIV
610 620 630 640 650
WSISSFLWFV TPTIVTAASF AYYIYVQGEV LTTPVAFTAL SLFTLLRDPL
660 670 680 690 700
DRLSDMLSFV VQSKVSLDRV QDFLNENDTK KYDQLTIDPN GNRFAFENST
710 720 730 740 750
ISWDKDNQDF KLKDLNIEFK TGKLNVVIGP TGSGKTSLLM ALLGEMYLLN
760 770 780 790 800
GKVVVPALEP RQELIVDANG TTNSIAYCSQ AAWLLNDTVK NNILFNSPFN
810 820 830 840 850
EARYKAVVEA CGLKRDFEIL KAGDLTEIGE KGITLSGGQK QRVSLARALY
860 870 880 890 900
SNARHVLLDD CLSAVDSHTA SWIYDNCITG PLMEDRTCIL VSHNIALTLR
910 920 930 940 950
NAELVVLLED GRVKDQGDPI DMLQKGLFGE DELVKSSILS RANSSANLAA
960 970 980 990 1000
KSSTSLSNLP AVKEQQVSVN NNSSHFEAKK LQKSLRTEAE RTEDGKLIKE
1010 1020 1030 1040 1050
ETKEEGVVGL DVYKWYLKIF GGWKIVSFLA SLFLIAQLLY IGQSWWVRAW
1060 1070 1080 1090 1100
ASHNVIAKII PRAQRAIAFI SKKASHLIDW RGSSQISMAS AENQPSSGHS
1110 1120 1130 1140 1150
TMYYLVLYLI IGFAQALLGA GKTILNFVAG INASRKIFNM ILNKVLHSKI
1160 1170 1180 1190 1200
RFFDATPTGR IMNRFSKDIE AIDQELTPYI QGAFYSLIEC LSTVILITFI
1210 1220 1230 1240 1250
TPQFLSVAIV VSILYYFVGY FYMAGSRELK RFESISRSPI YQHFSETLVG
1260 1270 1280 1290 1300
VTTIRAFGDE GRFMQENLHK IDENNKPFFY LWVANRWLAF RIDMIGSLVI
1310 1320 1330 1340 1350
FGAGLFILFN INNLDSGMAG ISLTYAISFT EGALWLVRLY SEVEMNMNSV
1360 1370 1380 1390 1400
ERVKEYMEIE QEPYNEHKEI PPPQWPQDGK IEVNDLSLRY APNLPRVIKN
1410 1420 1430 1440 1450
VSFSVDAQSK IGIVGRTGAG KSTIITALFR FLEPETGHIK IDNIDISGVD
1460 1470 1480 1490 1500
LQRLRRSITI IPQDPTLFSG TIKTNLDPYD EFSDRQIFEA LKRVNLISEE
1510 1520 1530 1540 1550
QLQQGATRET SNEASSTNSE NVNKFLDLSS EISEGGSNLS QGQRQLMCLA
1560 1570 1580 1590 1600
RSLLRSPKII LLDEATASID YSSDAKIQET IRKEFQGSTI LTIAHRLRSV
1610 1620 1630 1640 1650
IDYDKILVMD AGEVKEYDHP YSLLLNKQSA FYSMCEHSGE LDILIELAKK
1660
AFVEKLNSKK D
Length:1,661
Mass (Da):189,162
Last modified:December 15, 1998 - v2
Checksum:iE71D3D57A3013C38
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti172 – 1743TIT → QIH in AAA20992 (PubMed:8139928).Curated
Sequence conflicti188 – 1892LR → FS in AAA20992 (PubMed:8139928).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73153 Genomic DNA. Translation: CAA97500.1.
M88608 Genomic DNA. Translation: AAA20992.1.
BK006945 Genomic DNA. Translation: DAA09276.1.
PIRiS64800.
RefSeqiNP_013052.1. NM_001181868.1.

Genome annotation databases

EnsemblFungiiYLL048C; YLL048C; YLL048C.
GeneIDi850678.
KEGGisce:YLL048C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73153 Genomic DNA. Translation: CAA97500.1.
M88608 Genomic DNA. Translation: AAA20992.1.
BK006945 Genomic DNA. Translation: DAA09276.1.
PIRiS64800.
RefSeqiNP_013052.1. NM_001181868.1.

3D structure databases

ProteinModelPortaliP32386.
SMRiP32386. Positions 463-964, 1109-1634.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31267. 40 interactions.
DIPiDIP-6474N.
IntActiP32386. 8 interactions.
MINTiMINT-603723.

Protein family/group databases

TCDBi3.A.1.208.12. the atp-binding cassette (abc) superfamily.

PTM databases

iPTMnetiP32386.

Proteomic databases

MaxQBiP32386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL048C; YLL048C; YLL048C.
GeneIDi850678.
KEGGisce:YLL048C.

Organism-specific databases

EuPathDBiFungiDB:YLL048C.
SGDiS000003971. YBT1.

Phylogenomic databases

GeneTreeiENSGT00840000131000.
InParanoidiP32386.
OrthoDBiEOG70KGXT.

Enzyme and pathway databases

BioCyciYEAST:G3O-32147-MONOMER.

Miscellaneous databases

PROiP32386.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 3 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "RNP1, a new ribonucleoprotein gene of the yeast Saccharomyces cerevisiae."
    Cusick M.E.
    Nucleic Acids Res. 22:869-877(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-378.
  4. "A yeast ATP-binding cassette-type protein mediating ATP-dependent bile acid transport."
    Ortiz D.F., St Pierre M.V., Abdulmessih A., Arias I.M.
    J. Biol. Chem. 272:15358-15365(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-936, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-940 AND SER-955, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Vacuolar import of phosphatidylcholine requires the ATP-binding cassette transporter Ybt1."
    Gulshan K., Moye-Rowley W.S.
    Traffic 12:1257-1268(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION.
  11. "The Yeast vacuolar ABC transporter Ybt1p regulates membrane fusion through Ca2+ transport modulation."
    Sasser T.L., Padolina M., Fratti R.A.
    Biochem. J. 448:365-372(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION.

Entry informationi

Entry nameiYBT1_YEAST
AccessioniPrimary (citable) accession number: P32386
Secondary accession number(s): D6VXW0, Q07882
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: December 15, 1998
Last modified: June 8, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.