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P32383

- PLC1_YEAST

UniProt

P32383 - PLC1_YEAST

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1

Gene

PLC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for cell growth, osmoresistance and expression of GPD1.1 Publication

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei395 – 3951PROSITE-ProRule annotation
    Active sitei439 – 4391PROSITE-ProRule annotation
    Binding sitei518 – 5181SubstrateBy similarity
    Binding sitei520 – 5201SubstrateBy similarity
    Binding sitei614 – 6141SubstrateBy similarity
    Binding sitei643 – 6431SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi282 – 29312PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: SGD
    3. protein binding Source: IntAct
    4. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. inositol phosphate biosynthetic process Source: SGD
    2. intracellular signal transduction Source: InterPro
    3. phospholipid catabolic process Source: SGD
    4. protein localization to kinetochore Source: SGD
    5. signal transduction involved in filamentous growth Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:YPL268W-MONOMER.
    ReactomeiREACT_188945. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C
    Phospholipase C-1
    Short name:
    PLC-1
    Gene namesi
    Name:PLC1
    Ordered Locus Names:YPL268W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL268w.
    SGDiS000006189. PLC1.

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome, centromeric region Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8698691-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1PRO_0000088514Add
    BLAST

    Proteomic databases

    MaxQBiP32383.
    PaxDbiP32383.
    PRIDEiP32383.

    Expressioni

    Gene expression databases

    GenevestigatoriP32383.

    Interactioni

    Subunit structurei

    Interacts with SGD1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SGD1Q061323EBI-13485,EBI-34377

    Protein-protein interaction databases

    BioGridi35945. 85 interactions.
    IntActiP32383. 6 interactions.
    MINTiMINT-416735.
    STRINGi4932.YPL268W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32383.
    SMRiP32383. Positions 251-868.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini269 – 30436EF-handPROSITE-ProRule annotationAdd
    BLAST
    Domaini382 – 520139PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini590 – 709120PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini734 – 846113C2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    GeneTreeiENSGT00730000110782.
    HOGENOMiHOG000115576.
    KOiK05857.
    OMAiLFVHIMV.
    OrthoDBiEOG7NPG2M.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32383-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTESAIDDQR FNLTKELQRH SCRDQGKITQ KDDALDFISY SSFQSSFNTD    50
    QKSANNGSTV RRSIRSIFRR AAELPRVHMG PLTYSHGINE LVNKKLRKDC 100
    DLSTLCRVLQ RGIRMIRMTR RRRKFYEFKL INNNGQIIWK DGSKYLELDS 150
    VKDIRIGDTA STYQEEVDPK RLRSDSKLWI AIIYKVSNKL KALHVVALNE 200
    LDFNTFLSCI CGLVKLRREL MESILLPDNS QFARIHWQIT VSEKEEDEKK 250
    DTLSFADVKK LCDKFHIYVS TGQLLEFFQL ADINHNGLLN YFEFEKFIKI 300
    LKNRKEVNMI WSKFTKPPHS HLSFENFFQF LITEQHEQVD RQTAWSYFIK 350
    YREPTQLTMG QDGFTKFLKE QPYLVEVKEE LYSKPLNHYF IASSHNTYLL 400
    GKQIAETPSV EGYIQVLQQG CRCVEIDIWD GENGPVVCHG FLTSAIPLKT 450
    VIRVIKKYAF ITSPYPLIIS LEINCNKDNQ KLASLIMREV LAEQLYFVGT 500
    RTDKLPSPRE LKHKILLKSK KTSEATRGLS VNEPFPSSFS SSYESANEQE 550
    LRMKDDSTNS SSATNSSSMQ RIKRIGLKKH ADIINDVSNI SGIHGIKFRN 600
    FSLPESKTIA HCFSLNERKV EYMIKDKHLK LSLDKHNRRY LMRVYPHVLR 650
    YKSSNFNPIP FWKAGVQMVA TNWQTNDIGQ QLNLAMFQIL DHQPDGSFKS 700
    GYVLKPKKLL PVVTKAKMIP LIYEHFENGS DPVTVKIRIL STQLLPRLND 750
    TSPSRNNTNS FVKVEFHTDD EPTMPISIDK GTRISATEAS TKSSQGNGFN 800
    PIWDAEVSIT LKDTDLTFIK FMVISEETQI ASVCLKLNYL RMGYRHIPLF 850
    NMEGEQYIFC TLFIHTQIL 869
    Length:869
    Mass (Da):100,548
    Last modified:October 1, 1993 - v1
    Checksum:i13EE751259405E90
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti159 – 1591T → M in AAA99927. (PubMed:8395015)Curated
    Sequence conflicti181 – 1811A → T in AAA99927. (PubMed:8395015)Curated
    Sequence conflicti581 – 5811A → V in AAA99927. (PubMed:8395015)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D12738 Genomic DNA. Translation: BAA02230.1.
    L13036 Genomic DNA. Translation: AAA99927.1.
    S63468 Genomic DNA. Translation: AAB27349.2.
    Z73624 Genomic DNA. Translation: CAA98004.1.
    Z73623 Genomic DNA. Translation: CAA98003.1.
    BK006949 Genomic DNA. Translation: DAA11168.1.
    PIRiA47257.
    RefSeqiNP_015055.1. NM_001184082.1.

    Genome annotation databases

    EnsemblFungiiYPL268W; YPL268W; YPL268W.
    GeneIDi855860.
    KEGGisce:YPL268W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D12738 Genomic DNA. Translation: BAA02230.1 .
    L13036 Genomic DNA. Translation: AAA99927.1 .
    S63468 Genomic DNA. Translation: AAB27349.2 .
    Z73624 Genomic DNA. Translation: CAA98004.1 .
    Z73623 Genomic DNA. Translation: CAA98003.1 .
    BK006949 Genomic DNA. Translation: DAA11168.1 .
    PIRi A47257.
    RefSeqi NP_015055.1. NM_001184082.1.

    3D structure databases

    ProteinModelPortali P32383.
    SMRi P32383. Positions 251-868.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35945. 85 interactions.
    IntActi P32383. 6 interactions.
    MINTi MINT-416735.
    STRINGi 4932.YPL268W.

    Proteomic databases

    MaxQBi P32383.
    PaxDbi P32383.
    PRIDEi P32383.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL268W ; YPL268W ; YPL268W .
    GeneIDi 855860.
    KEGGi sce:YPL268W.

    Organism-specific databases

    CYGDi YPL268w.
    SGDi S000006189. PLC1.

    Phylogenomic databases

    eggNOGi NOG149692.
    GeneTreei ENSGT00730000110782.
    HOGENOMi HOG000115576.
    KOi K05857.
    OMAi LFVHIMV.
    OrthoDBi EOG7NPG2M.

    Enzyme and pathway databases

    BioCyci YEAST:YPL268W-MONOMER.
    Reactomei REACT_188945. Synthesis of IP3 and IP4 in the cytosol.

    Miscellaneous databases

    NextBioi 980475.

    Gene expression databases

    Genevestigatori P32383.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The putative phosphoinositide-specific phospholipase C gene, PLC1, of the yeast Saccharomyces cerevisiae is important for cell growth."
      Yoko-O T., Matsui Y., Yagisawa H., Nojima H., Uno I., Toh-e A.
      Proc. Natl. Acad. Sci. U.S.A. 90:1804-1808(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae."
      Flick J.S., Thorner J.W.
      Mol. Cell. Biol. 13:5861-5876(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "A mutation in PLC1, a candidate phosphoinositide-specific phospholipase C gene from Saccharomyces cerevisiae, causes aberrant mitotic chromosome segregation."
      Payne W.E., Fitzgerald-Hayes M.
      Mol. Cell. Biol. 13:4351-4364(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae."
      Lin H., Nguyen P.H., Vancura A.
      Mol. Genet. Genomics 267:313-320(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SGD1.

    Entry informationi

    Entry nameiPLC1_YEAST
    AccessioniPrimary (citable) accession number: P32383
    Secondary accession number(s): D6W3A2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 144 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3