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P32383 (PLC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1

EC=3.1.4.11
Alternative name(s):
Phosphoinositide phospholipase C
Phospholipase C-1
Short name=PLC-1
Gene names
Name:PLC1
Ordered Locus Names:YPL268W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length869 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for cell growth, osmoresistance and expression of GPD1. Ref.6

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Subunit structure

Interacts with SGD1. Ref.6

Sequence similarities

Contains 1 C2 domain.

Contains 1 EF-hand domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SGD1Q061323EBI-13485,EBI-34377

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8698691-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1
PRO_0000088514

Regions

Domain269 – 30436EF-hand
Domain382 – 520139PI-PLC X-box
Domain590 – 709120PI-PLC Y-box
Domain734 – 846113C2
Calcium binding282 – 29312 Potential

Sites

Active site3951 By similarity
Active site4391 By similarity
Binding site5181Substrate By similarity
Binding site5201Substrate By similarity
Binding site6141Substrate By similarity
Binding site6431Substrate By similarity

Experimental info

Sequence conflict1591T → M in AAA99927. Ref.2
Sequence conflict1811A → T in AAA99927. Ref.2
Sequence conflict5811A → V in AAA99927. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P32383 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 13EE751259405E90

FASTA869100,548
        10         20         30         40         50         60 
MTESAIDDQR FNLTKELQRH SCRDQGKITQ KDDALDFISY SSFQSSFNTD QKSANNGSTV 

        70         80         90        100        110        120 
RRSIRSIFRR AAELPRVHMG PLTYSHGINE LVNKKLRKDC DLSTLCRVLQ RGIRMIRMTR 

       130        140        150        160        170        180 
RRRKFYEFKL INNNGQIIWK DGSKYLELDS VKDIRIGDTA STYQEEVDPK RLRSDSKLWI 

       190        200        210        220        230        240 
AIIYKVSNKL KALHVVALNE LDFNTFLSCI CGLVKLRREL MESILLPDNS QFARIHWQIT 

       250        260        270        280        290        300 
VSEKEEDEKK DTLSFADVKK LCDKFHIYVS TGQLLEFFQL ADINHNGLLN YFEFEKFIKI 

       310        320        330        340        350        360 
LKNRKEVNMI WSKFTKPPHS HLSFENFFQF LITEQHEQVD RQTAWSYFIK YREPTQLTMG 

       370        380        390        400        410        420 
QDGFTKFLKE QPYLVEVKEE LYSKPLNHYF IASSHNTYLL GKQIAETPSV EGYIQVLQQG 

       430        440        450        460        470        480 
CRCVEIDIWD GENGPVVCHG FLTSAIPLKT VIRVIKKYAF ITSPYPLIIS LEINCNKDNQ 

       490        500        510        520        530        540 
KLASLIMREV LAEQLYFVGT RTDKLPSPRE LKHKILLKSK KTSEATRGLS VNEPFPSSFS 

       550        560        570        580        590        600 
SSYESANEQE LRMKDDSTNS SSATNSSSMQ RIKRIGLKKH ADIINDVSNI SGIHGIKFRN 

       610        620        630        640        650        660 
FSLPESKTIA HCFSLNERKV EYMIKDKHLK LSLDKHNRRY LMRVYPHVLR YKSSNFNPIP 

       670        680        690        700        710        720 
FWKAGVQMVA TNWQTNDIGQ QLNLAMFQIL DHQPDGSFKS GYVLKPKKLL PVVTKAKMIP 

       730        740        750        760        770        780 
LIYEHFENGS DPVTVKIRIL STQLLPRLND TSPSRNNTNS FVKVEFHTDD EPTMPISIDK 

       790        800        810        820        830        840 
GTRISATEAS TKSSQGNGFN PIWDAEVSIT LKDTDLTFIK FMVISEETQI ASVCLKLNYL 

       850        860 
RMGYRHIPLF NMEGEQYIFC TLFIHTQIL 

« Hide

References

« Hide 'large scale' references
[1]"The putative phosphoinositide-specific phospholipase C gene, PLC1, of the yeast Saccharomyces cerevisiae is important for cell growth."
Yoko-O T., Matsui Y., Yagisawa H., Nojima H., Uno I., Toh-e A.
Proc. Natl. Acad. Sci. U.S.A. 90:1804-1808(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae."
Flick J.S., Thorner J.W.
Mol. Cell. Biol. 13:5861-5876(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A mutation in PLC1, a candidate phosphoinositide-specific phospholipase C gene from Saccharomyces cerevisiae, causes aberrant mitotic chromosome segregation."
Payne W.E., Fitzgerald-Hayes M.
Mol. Cell. Biol. 13:4351-4364(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae."
Lin H., Nguyen P.H., Vancura A.
Mol. Genet. Genomics 267:313-320(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SGD1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D12738 Genomic DNA. Translation: BAA02230.1.
L13036 Genomic DNA. Translation: AAA99927.1.
S63468 Genomic DNA. Translation: AAB27349.2.
Z73624 Genomic DNA. Translation: CAA98004.1.
Z73623 Genomic DNA. Translation: CAA98003.1.
BK006949 Genomic DNA. Translation: DAA11168.1.
PIRA47257.
RefSeqNP_015055.1. NM_001184082.1.

3D structure databases

ProteinModelPortalP32383.
SMRP32383. Positions 108-869.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35945. 84 interactions.
IntActP32383. 6 interactions.
MINTMINT-416735.
STRING4932.YPL268W.

Proteomic databases

PaxDbP32383.
PRIDEP32383.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL268W; YPL268W; YPL268W.
GeneID855860.
KEGGsce:YPL268W.

Organism-specific databases

CYGDYPL268w.
SGDS000006189. PLC1.

Phylogenomic databases

eggNOGNOG149692.
GeneTreeENSGT00730000110782.
HOGENOMHOG000115576.
KOK05857.
OMAQKTEHWI.
OrthoDBEOG7NPG2M.

Enzyme and pathway databases

BioCycYEAST:YPL268W-MONOMER.

Gene expression databases

GenevestigatorP32383.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028409. Plc1.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF20. PTHR10336:SF20. 1 hit.
PfamPF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
SMARTSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980475.

Entry information

Entry namePLC1_YEAST
AccessionPrimary (citable) accession number: P32383
Secondary accession number(s): D6W3A2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families