P32383 (PLC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1
EC=3.1.4.11

Alternative name(s):
Phosphoinositide phospholipase C
Phospholipase C-1
Short name=PLC-1

Gene names

Name:	PLC1
Ordered Locus Names:	YPL268W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	869 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for cell growth, osmoresistance and expression of GPD1. Ref.6
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Subunit structure	Interacts with SGD1. Ref.6
Sequence similarities	Contains 1 C2 domain. Contains 1 EF-hand domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inositol phosphate biosynthetic process Inferred from mutant phenotype PubMed 10390371. Source: SGD intracellular signal transduction Inferred from electronic annotation. Source: InterPro phospholipid catabolic process Inferred from direct assay Ref.2. Source: SGD protein localization to kinetochore Inferred from mutant phenotype PubMed 19205744. Source: SGD signal transduction involved in filamentous growth Inferred from genetic interaction PubMed 10514491. Source: SGD
Cellular_component	condensed nuclear chromosome kinetochore Inferred from physical interaction PubMed 10779349. Source: SGD
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro phosphatidylinositol phospholipase C activity Inferred from direct assay PubMed 15485855Ref.2. Source: SGD signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SGD1	Q06132	3	EBI-13485,EBI-34377

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 869	869	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1		PRO_0000088514
Regions
	Domain	269 – 304	36	EF-hand
	Domain	382 – 520	139	PI-PLC X-box
	Domain	590 – 709	120	PI-PLC Y-box
	Domain	734 – 846	113	C2
	Calcium binding	282 – 293	12	Potential
Sites
	Active site	395	1	By similarity
	Active site	439	1	By similarity
	Binding site	518	1	Substrate By similarity
	Binding site	520	1	Substrate By similarity
	Binding site	614	1	Substrate By similarity
	Binding site	643	1	Substrate By similarity
Experimental info
	Sequence conflict	159	1	T → M in AAA99927. Ref.2
	Sequence conflict	181	1	A → T in AAA99927. Ref.2
	Sequence conflict	581	1	A → V in AAA99927. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32383 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 13EE751259405E90	FASTA	869	100,548
	10 20 30 40 50 60 MTESAIDDQR FNLTKELQRH SCRDQGKITQ KDDALDFISY SSFQSSFNTD QKSANNGSTV 70 80 90 100 110 120 RRSIRSIFRR AAELPRVHMG PLTYSHGINE LVNKKLRKDC DLSTLCRVLQ RGIRMIRMTR 130 140 150 160 170 180 RRRKFYEFKL INNNGQIIWK DGSKYLELDS VKDIRIGDTA STYQEEVDPK RLRSDSKLWI 190 200 210 220 230 240 AIIYKVSNKL KALHVVALNE LDFNTFLSCI CGLVKLRREL MESILLPDNS QFARIHWQIT 250 260 270 280 290 300 VSEKEEDEKK DTLSFADVKK LCDKFHIYVS TGQLLEFFQL ADINHNGLLN YFEFEKFIKI 310 320 330 340 350 360 LKNRKEVNMI WSKFTKPPHS HLSFENFFQF LITEQHEQVD RQTAWSYFIK YREPTQLTMG 370 380 390 400 410 420 QDGFTKFLKE QPYLVEVKEE LYSKPLNHYF IASSHNTYLL GKQIAETPSV EGYIQVLQQG 430 440 450 460 470 480 CRCVEIDIWD GENGPVVCHG FLTSAIPLKT VIRVIKKYAF ITSPYPLIIS LEINCNKDNQ 490 500 510 520 530 540 KLASLIMREV LAEQLYFVGT RTDKLPSPRE LKHKILLKSK KTSEATRGLS VNEPFPSSFS 550 560 570 580 590 600 SSYESANEQE LRMKDDSTNS SSATNSSSMQ RIKRIGLKKH ADIINDVSNI SGIHGIKFRN 610 620 630 640 650 660 FSLPESKTIA HCFSLNERKV EYMIKDKHLK LSLDKHNRRY LMRVYPHVLR YKSSNFNPIP 670 680 690 700 710 720 FWKAGVQMVA TNWQTNDIGQ QLNLAMFQIL DHQPDGSFKS GYVLKPKKLL PVVTKAKMIP 730 740 750 760 770 780 LIYEHFENGS DPVTVKIRIL STQLLPRLND TSPSRNNTNS FVKVEFHTDD EPTMPISIDK 790 800 810 820 830 840 GTRISATEAS TKSSQGNGFN PIWDAEVSIT LKDTDLTFIK FMVISEETQI ASVCLKLNYL 850 860 RMGYRHIPLF NMEGEQYIFC TLFIHTQIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The putative phosphoinositide-specific phospholipase C gene, PLC1, of the yeast Saccharomyces cerevisiae is important for cell growth." Yoko-O T., Matsui Y., Yagisawa H., Nojima H., Uno I., Toh-e A. Proc. Natl. Acad. Sci. U.S.A. 90:1804-1808(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae." Flick J.S., Thorner J.W. Mol. Cell. Biol. 13:5861-5876(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A mutation in PLC1, a candidate phosphoinositide-specific phospholipase C gene from Saccharomyces cerevisiae, causes aberrant mitotic chromosome segregation." Payne W.E., Fitzgerald-Hayes M. Mol. Cell. Biol. 13:4351-4364(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae." Lin H., Nguyen P.H., Vancura A. Mol. Genet. Genomics 267:313-320(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SGD1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12738 Genomic DNA. Translation: BAA02230.1. L13036 Genomic DNA. Translation: AAA99927.1. S63468 Genomic DNA. Translation: AAB27349.2. Z73624 Genomic DNA. Translation: CAA98004.1. Z73623 Genomic DNA. Translation: CAA98003.1. BK006949 Genomic DNA. Translation: DAA11168.1.
PIR	A47257.
RefSeq	NP_015055.1. NM_001184082.1.
3D structure databases
ProteinModelPortal	P32383.
SMR	P32383. Positions 251-868.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32383. 4 interactions.
MINT	MINT-416735.
STRING	4932.YPL268W.
Proteomic databases
PaxDb	P32383.
PRIDE	P32383.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL268W; YPL268W; YPL268W.
GeneID	855860.
KEGG	sce:YPL268W.
Organism-specific databases
CYGD	YPL268w.
SGD	S000006189. PLC1.
Phylogenomic databases
eggNOG	NOG149692.
GeneTree	ENSGT00670000098052.
HOGENOM	HOG000115576.
KO	K05857.
OMA	NGPVVCH.
OrthoDB	EOG441TKH.
Gene expression databases
Genevestigator	P32383.
GermOnline	YPL268W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.238.10. 2 hits. 2.30.29.30. 1 hit. 3.20.20.190. 2 hits.
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR011993. PH_like_dom. IPR001192. Pinositol_PLipase_C. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR000909. PLipase_C_PInositol-sp_X_dom. IPR001711. PLipase_C_Pinositol-sp_Y. [Graphical view]
PANTHER	PTHR10336. PTHR10336. 1 hit.
Pfam	PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF51695. PLC-like_Pdiesterase_TIM-brl. 1 hit.
PROSITE	PS50004. C2. 1 hit. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980475.

Entry information

Entry name

PLC1_YEAST

Accession

Primary (citable) accession number: P32383
Secondary accession number(s): D6W3A2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 130 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program