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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1

Gene

PLC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for cell growth, osmoresistance and expression of GPD1.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei395PROSITE-ProRule annotation1
Active sitei439PROSITE-ProRule annotation1
Binding sitei518SubstrateBy similarity1
Binding sitei520SubstrateBy similarity1
Binding sitei614SubstrateBy similarity1
Binding sitei643SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi282 – 293PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • inositol phosphate biosynthetic process Source: SGD
  • intracellular signal transduction Source: InterPro
  • phospholipid catabolic process Source: SGD
  • protein localization to kinetochore Source: SGD
  • signal transduction involved in filamentous growth Source: SGD

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YPL268W-MONOMER
BRENDAi3.1.4.11 984
ReactomeiR-SCE-114604 GPVI-mediated activation cascade
R-SCE-1169408 ISG15 antiviral mechanism
R-SCE-1855204 Synthesis of IP3 and IP4 in the cytosol
R-SCE-202433 Generation of second messenger molecules
R-SCE-5607764 CLEC7A (Dectin-1) signaling

Chemistry databases

SwissLipidsiSLP:000000122

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C
Phospholipase C-1
Short name:
PLC-1
Gene namesi
Name:PLC1
Ordered Locus Names:YPL268W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YPL268W
SGDiS000006189 PLC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000885141 – 8691-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1Add BLAST869

Proteomic databases

MaxQBiP32383
PaxDbiP32383
PRIDEiP32383

Interactioni

Subunit structurei

Interacts with SGD1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SGD1Q061323EBI-13485,EBI-34377

Protein-protein interaction databases

BioGridi35945, 98 interactors
IntActiP32383, 6 interactors
MINTiP32383
STRINGi4932.YPL268W

Structurei

3D structure databases

ProteinModelPortaliP32383
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini269 – 304EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini382 – 520PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST139
Domaini590 – 709PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST120
Domaini734 – 846C2PROSITE-ProRule annotationAdd BLAST113

Phylogenomic databases

GeneTreeiENSGT00730000110782
HOGENOMiHOG000115576
InParanoidiP32383
KOiK05857
OMAiCKTIWQE
OrthoDBiEOG092C0GR7

Family and domain databases

CDDicd13360 PH_PLC_fungal, 1 hit
Gene3Di2.60.40.150, 1 hit
3.20.20.190, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR001192 PI-PLC_fam
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR037755 Plc1_PH
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PfamiView protein in Pfam
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

P32383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESAIDDQR FNLTKELQRH SCRDQGKITQ KDDALDFISY SSFQSSFNTD
60 70 80 90 100
QKSANNGSTV RRSIRSIFRR AAELPRVHMG PLTYSHGINE LVNKKLRKDC
110 120 130 140 150
DLSTLCRVLQ RGIRMIRMTR RRRKFYEFKL INNNGQIIWK DGSKYLELDS
160 170 180 190 200
VKDIRIGDTA STYQEEVDPK RLRSDSKLWI AIIYKVSNKL KALHVVALNE
210 220 230 240 250
LDFNTFLSCI CGLVKLRREL MESILLPDNS QFARIHWQIT VSEKEEDEKK
260 270 280 290 300
DTLSFADVKK LCDKFHIYVS TGQLLEFFQL ADINHNGLLN YFEFEKFIKI
310 320 330 340 350
LKNRKEVNMI WSKFTKPPHS HLSFENFFQF LITEQHEQVD RQTAWSYFIK
360 370 380 390 400
YREPTQLTMG QDGFTKFLKE QPYLVEVKEE LYSKPLNHYF IASSHNTYLL
410 420 430 440 450
GKQIAETPSV EGYIQVLQQG CRCVEIDIWD GENGPVVCHG FLTSAIPLKT
460 470 480 490 500
VIRVIKKYAF ITSPYPLIIS LEINCNKDNQ KLASLIMREV LAEQLYFVGT
510 520 530 540 550
RTDKLPSPRE LKHKILLKSK KTSEATRGLS VNEPFPSSFS SSYESANEQE
560 570 580 590 600
LRMKDDSTNS SSATNSSSMQ RIKRIGLKKH ADIINDVSNI SGIHGIKFRN
610 620 630 640 650
FSLPESKTIA HCFSLNERKV EYMIKDKHLK LSLDKHNRRY LMRVYPHVLR
660 670 680 690 700
YKSSNFNPIP FWKAGVQMVA TNWQTNDIGQ QLNLAMFQIL DHQPDGSFKS
710 720 730 740 750
GYVLKPKKLL PVVTKAKMIP LIYEHFENGS DPVTVKIRIL STQLLPRLND
760 770 780 790 800
TSPSRNNTNS FVKVEFHTDD EPTMPISIDK GTRISATEAS TKSSQGNGFN
810 820 830 840 850
PIWDAEVSIT LKDTDLTFIK FMVISEETQI ASVCLKLNYL RMGYRHIPLF
860
NMEGEQYIFC TLFIHTQIL
Length:869
Mass (Da):100,548
Last modified:October 1, 1993 - v1
Checksum:i13EE751259405E90
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159T → M in AAA99927 (PubMed:8395015).Curated1
Sequence conflicti181A → T in AAA99927 (PubMed:8395015).Curated1
Sequence conflicti581A → V in AAA99927 (PubMed:8395015).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12738 Genomic DNA Translation: BAA02230.1
L13036 Genomic DNA Translation: AAA99927.1
S63468 Genomic DNA Translation: AAB27349.2
Z73624 Genomic DNA Translation: CAA98004.1
Z73623 Genomic DNA Translation: CAA98003.1
BK006949 Genomic DNA Translation: DAA11168.1
PIRiA47257
RefSeqiNP_015055.1, NM_001184082.1

Genome annotation databases

EnsemblFungiiYPL268W; YPL268W; YPL268W
GeneIDi855860
KEGGisce:YPL268W

Entry informationi

Entry nameiPLC1_YEAST
AccessioniPrimary (citable) accession number: P32383
Secondary accession number(s): D6W3A2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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