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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1

Gene

PLC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for cell growth, osmoresistance and expression of GPD1.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei395PROSITE-ProRule annotation1
Active sitei439PROSITE-ProRule annotation1
Binding sitei518SubstrateBy similarity1
Binding sitei520SubstrateBy similarity1
Binding sitei614SubstrateBy similarity1
Binding sitei643SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi282 – 293PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • inositol phosphate biosynthetic process Source: SGD
  • intracellular signal transduction Source: InterPro
  • phospholipid catabolic process Source: SGD
  • protein localization to kinetochore Source: SGD
  • signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YPL268W-MONOMER.
BRENDAi3.1.4.11. 984.
ReactomeiR-SCE-114604. GPVI-mediated activation cascade.
R-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-SCE-202433. Generation of second messenger molecules.
R-SCE-5607764. CLEC7A (Dectin-1) signaling.

Chemistry databases

SwissLipidsiSLP:000000122.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C
Phospholipase C-1
Short name:
PLC-1
Gene namesi
Name:PLC1
Ordered Locus Names:YPL268W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL268W.
SGDiS000006189. PLC1.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: SGD
  • condensed nuclear chromosome kinetochore Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000885141 – 8691-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1Add BLAST869

Proteomic databases

MaxQBiP32383.
PRIDEiP32383.

Interactioni

Subunit structurei

Interacts with SGD1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SGD1Q061323EBI-13485,EBI-34377

Protein-protein interaction databases

BioGridi35945. 96 interactors.
IntActiP32383. 6 interactors.
MINTiMINT-416735.

Structurei

3D structure databases

ProteinModelPortaliP32383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini269 – 304EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini382 – 520PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST139
Domaini590 – 709PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST120
Domaini734 – 846C2PROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00730000110782.
HOGENOMiHOG000115576.
InParanoidiP32383.
KOiK05857.
OMAiVYTCSVP.
OrthoDBiEOG092C0GR7.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_dom-like.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESAIDDQR FNLTKELQRH SCRDQGKITQ KDDALDFISY SSFQSSFNTD
60 70 80 90 100
QKSANNGSTV RRSIRSIFRR AAELPRVHMG PLTYSHGINE LVNKKLRKDC
110 120 130 140 150
DLSTLCRVLQ RGIRMIRMTR RRRKFYEFKL INNNGQIIWK DGSKYLELDS
160 170 180 190 200
VKDIRIGDTA STYQEEVDPK RLRSDSKLWI AIIYKVSNKL KALHVVALNE
210 220 230 240 250
LDFNTFLSCI CGLVKLRREL MESILLPDNS QFARIHWQIT VSEKEEDEKK
260 270 280 290 300
DTLSFADVKK LCDKFHIYVS TGQLLEFFQL ADINHNGLLN YFEFEKFIKI
310 320 330 340 350
LKNRKEVNMI WSKFTKPPHS HLSFENFFQF LITEQHEQVD RQTAWSYFIK
360 370 380 390 400
YREPTQLTMG QDGFTKFLKE QPYLVEVKEE LYSKPLNHYF IASSHNTYLL
410 420 430 440 450
GKQIAETPSV EGYIQVLQQG CRCVEIDIWD GENGPVVCHG FLTSAIPLKT
460 470 480 490 500
VIRVIKKYAF ITSPYPLIIS LEINCNKDNQ KLASLIMREV LAEQLYFVGT
510 520 530 540 550
RTDKLPSPRE LKHKILLKSK KTSEATRGLS VNEPFPSSFS SSYESANEQE
560 570 580 590 600
LRMKDDSTNS SSATNSSSMQ RIKRIGLKKH ADIINDVSNI SGIHGIKFRN
610 620 630 640 650
FSLPESKTIA HCFSLNERKV EYMIKDKHLK LSLDKHNRRY LMRVYPHVLR
660 670 680 690 700
YKSSNFNPIP FWKAGVQMVA TNWQTNDIGQ QLNLAMFQIL DHQPDGSFKS
710 720 730 740 750
GYVLKPKKLL PVVTKAKMIP LIYEHFENGS DPVTVKIRIL STQLLPRLND
760 770 780 790 800
TSPSRNNTNS FVKVEFHTDD EPTMPISIDK GTRISATEAS TKSSQGNGFN
810 820 830 840 850
PIWDAEVSIT LKDTDLTFIK FMVISEETQI ASVCLKLNYL RMGYRHIPLF
860
NMEGEQYIFC TLFIHTQIL
Length:869
Mass (Da):100,548
Last modified:October 1, 1993 - v1
Checksum:i13EE751259405E90
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159T → M in AAA99927 (PubMed:8395015).Curated1
Sequence conflicti181A → T in AAA99927 (PubMed:8395015).Curated1
Sequence conflicti581A → V in AAA99927 (PubMed:8395015).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12738 Genomic DNA. Translation: BAA02230.1.
L13036 Genomic DNA. Translation: AAA99927.1.
S63468 Genomic DNA. Translation: AAB27349.2.
Z73624 Genomic DNA. Translation: CAA98004.1.
Z73623 Genomic DNA. Translation: CAA98003.1.
BK006949 Genomic DNA. Translation: DAA11168.1.
PIRiA47257.
RefSeqiNP_015055.1. NM_001184082.1.

Genome annotation databases

EnsemblFungiiYPL268W; YPL268W; YPL268W.
GeneIDi855860.
KEGGisce:YPL268W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12738 Genomic DNA. Translation: BAA02230.1.
L13036 Genomic DNA. Translation: AAA99927.1.
S63468 Genomic DNA. Translation: AAB27349.2.
Z73624 Genomic DNA. Translation: CAA98004.1.
Z73623 Genomic DNA. Translation: CAA98003.1.
BK006949 Genomic DNA. Translation: DAA11168.1.
PIRiA47257.
RefSeqiNP_015055.1. NM_001184082.1.

3D structure databases

ProteinModelPortaliP32383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35945. 96 interactors.
IntActiP32383. 6 interactors.
MINTiMINT-416735.

Chemistry databases

SwissLipidsiSLP:000000122.

Proteomic databases

MaxQBiP32383.
PRIDEiP32383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL268W; YPL268W; YPL268W.
GeneIDi855860.
KEGGisce:YPL268W.

Organism-specific databases

EuPathDBiFungiDB:YPL268W.
SGDiS000006189. PLC1.

Phylogenomic databases

GeneTreeiENSGT00730000110782.
HOGENOMiHOG000115576.
InParanoidiP32383.
KOiK05857.
OMAiVYTCSVP.
OrthoDBiEOG092C0GR7.

Enzyme and pathway databases

BioCyciYEAST:YPL268W-MONOMER.
BRENDAi3.1.4.11. 984.
ReactomeiR-SCE-114604. GPVI-mediated activation cascade.
R-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-SCE-202433. Generation of second messenger molecules.
R-SCE-5607764. CLEC7A (Dectin-1) signaling.

Miscellaneous databases

PROiP32383.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_dom-like.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLC1_YEAST
AccessioniPrimary (citable) accession number: P32383
Secondary accession number(s): D6W3A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.