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P32383

- PLC1_YEAST

UniProt

P32383 - PLC1_YEAST

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1

Gene

PLC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Required for cell growth, osmoresistance and expression of GPD1.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei395 – 3951PROSITE-ProRule annotation
Active sitei439 – 4391PROSITE-ProRule annotation
Binding sitei518 – 5181SubstrateBy similarity
Binding sitei520 – 5201SubstrateBy similarity
Binding sitei614 – 6141SubstrateBy similarity
Binding sitei643 – 6431SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi282 – 29312PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: SGD
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. inositol phosphate biosynthetic process Source: SGD
  2. intracellular signal transduction Source: InterPro
  3. phospholipid catabolic process Source: SGD
  4. protein localization to kinetochore Source: SGD
  5. signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YPL268W-MONOMER.
ReactomeiREACT_188945. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C
Phospholipase C-1
Short name:
PLC-1
Gene namesi
Name:PLC1
Ordered Locus Names:YPL268W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL268w.
SGDiS000006189. PLC1.

Subcellular locationi

GO - Cellular componenti

  1. chromosome, centromeric region Source: SGD
  2. condensed nuclear chromosome kinetochore Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8698691-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1PRO_0000088514Add
BLAST

Proteomic databases

MaxQBiP32383.
PaxDbiP32383.
PRIDEiP32383.

Expressioni

Gene expression databases

GenevestigatoriP32383.

Interactioni

Subunit structurei

Interacts with SGD1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SGD1Q061323EBI-13485,EBI-34377

Protein-protein interaction databases

BioGridi35945. 85 interactions.
IntActiP32383. 6 interactions.
MINTiMINT-416735.
STRINGi4932.YPL268W.

Structurei

3D structure databases

ProteinModelPortaliP32383.
SMRiP32383. Positions 251-868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini269 – 30436EF-handPROSITE-ProRule annotationAdd
BLAST
Domaini382 – 520139PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini590 – 709120PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini734 – 846113C2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00730000110782.
HOGENOMiHOG000115576.
InParanoidiP32383.
KOiK05857.
OMAiLFVHIMV.
OrthoDBiEOG7NPG2M.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32383 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTESAIDDQR FNLTKELQRH SCRDQGKITQ KDDALDFISY SSFQSSFNTD
60 70 80 90 100
QKSANNGSTV RRSIRSIFRR AAELPRVHMG PLTYSHGINE LVNKKLRKDC
110 120 130 140 150
DLSTLCRVLQ RGIRMIRMTR RRRKFYEFKL INNNGQIIWK DGSKYLELDS
160 170 180 190 200
VKDIRIGDTA STYQEEVDPK RLRSDSKLWI AIIYKVSNKL KALHVVALNE
210 220 230 240 250
LDFNTFLSCI CGLVKLRREL MESILLPDNS QFARIHWQIT VSEKEEDEKK
260 270 280 290 300
DTLSFADVKK LCDKFHIYVS TGQLLEFFQL ADINHNGLLN YFEFEKFIKI
310 320 330 340 350
LKNRKEVNMI WSKFTKPPHS HLSFENFFQF LITEQHEQVD RQTAWSYFIK
360 370 380 390 400
YREPTQLTMG QDGFTKFLKE QPYLVEVKEE LYSKPLNHYF IASSHNTYLL
410 420 430 440 450
GKQIAETPSV EGYIQVLQQG CRCVEIDIWD GENGPVVCHG FLTSAIPLKT
460 470 480 490 500
VIRVIKKYAF ITSPYPLIIS LEINCNKDNQ KLASLIMREV LAEQLYFVGT
510 520 530 540 550
RTDKLPSPRE LKHKILLKSK KTSEATRGLS VNEPFPSSFS SSYESANEQE
560 570 580 590 600
LRMKDDSTNS SSATNSSSMQ RIKRIGLKKH ADIINDVSNI SGIHGIKFRN
610 620 630 640 650
FSLPESKTIA HCFSLNERKV EYMIKDKHLK LSLDKHNRRY LMRVYPHVLR
660 670 680 690 700
YKSSNFNPIP FWKAGVQMVA TNWQTNDIGQ QLNLAMFQIL DHQPDGSFKS
710 720 730 740 750
GYVLKPKKLL PVVTKAKMIP LIYEHFENGS DPVTVKIRIL STQLLPRLND
760 770 780 790 800
TSPSRNNTNS FVKVEFHTDD EPTMPISIDK GTRISATEAS TKSSQGNGFN
810 820 830 840 850
PIWDAEVSIT LKDTDLTFIK FMVISEETQI ASVCLKLNYL RMGYRHIPLF
860
NMEGEQYIFC TLFIHTQIL
Length:869
Mass (Da):100,548
Last modified:October 1, 1993 - v1
Checksum:i13EE751259405E90
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti159 – 1591T → M in AAA99927. (PubMed:8395015)Curated
Sequence conflicti181 – 1811A → T in AAA99927. (PubMed:8395015)Curated
Sequence conflicti581 – 5811A → V in AAA99927. (PubMed:8395015)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D12738 Genomic DNA. Translation: BAA02230.1.
L13036 Genomic DNA. Translation: AAA99927.1.
S63468 Genomic DNA. Translation: AAB27349.2.
Z73624 Genomic DNA. Translation: CAA98004.1.
Z73623 Genomic DNA. Translation: CAA98003.1.
BK006949 Genomic DNA. Translation: DAA11168.1.
PIRiA47257.
RefSeqiNP_015055.1. NM_001184082.1.

Genome annotation databases

EnsemblFungiiYPL268W; YPL268W; YPL268W.
GeneIDi855860.
KEGGisce:YPL268W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D12738 Genomic DNA. Translation: BAA02230.1 .
L13036 Genomic DNA. Translation: AAA99927.1 .
S63468 Genomic DNA. Translation: AAB27349.2 .
Z73624 Genomic DNA. Translation: CAA98004.1 .
Z73623 Genomic DNA. Translation: CAA98003.1 .
BK006949 Genomic DNA. Translation: DAA11168.1 .
PIRi A47257.
RefSeqi NP_015055.1. NM_001184082.1.

3D structure databases

ProteinModelPortali P32383.
SMRi P32383. Positions 251-868.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35945. 85 interactions.
IntActi P32383. 6 interactions.
MINTi MINT-416735.
STRINGi 4932.YPL268W.

Proteomic databases

MaxQBi P32383.
PaxDbi P32383.
PRIDEi P32383.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL268W ; YPL268W ; YPL268W .
GeneIDi 855860.
KEGGi sce:YPL268W.

Organism-specific databases

CYGDi YPL268w.
SGDi S000006189. PLC1.

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00730000110782.
HOGENOMi HOG000115576.
InParanoidi P32383.
KOi K05857.
OMAi LFVHIMV.
OrthoDBi EOG7NPG2M.

Enzyme and pathway databases

BioCyci YEAST:YPL268W-MONOMER.
Reactomei REACT_188945. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

NextBioi 980475.

Gene expression databases

Genevestigatori P32383.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
Pfami PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The putative phosphoinositide-specific phospholipase C gene, PLC1, of the yeast Saccharomyces cerevisiae is important for cell growth."
    Yoko-O T., Matsui Y., Yagisawa H., Nojima H., Uno I., Toh-e A.
    Proc. Natl. Acad. Sci. U.S.A. 90:1804-1808(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae."
    Flick J.S., Thorner J.W.
    Mol. Cell. Biol. 13:5861-5876(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A mutation in PLC1, a candidate phosphoinositide-specific phospholipase C gene from Saccharomyces cerevisiae, causes aberrant mitotic chromosome segregation."
    Payne W.E., Fitzgerald-Hayes M.
    Mol. Cell. Biol. 13:4351-4364(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae."
    Lin H., Nguyen P.H., Vancura A.
    Mol. Genet. Genomics 267:313-320(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SGD1.

Entry informationi

Entry nameiPLC1_YEAST
AccessioniPrimary (citable) accession number: P32383
Secondary accession number(s): D6W3A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3