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P32381

- ARP2_YEAST

UniProt

P32381 - ARP2_YEAST

Protein

Actin-related protein 2

Gene

ARP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi159 – 1613ATPBy similarity
    Nucleotide bindingi213 – 2175ATPBy similarity
    Nucleotide bindingi304 – 3096ATPBy similarity

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. actin filament organization Source: SGD
    2. Arp2/3 complex-mediated actin nucleation Source: SGD
    3. ascospore wall assembly Source: SGD
    4. ATP catabolic process Source: SGD
    5. CVT pathway Source: SGD
    6. establishment of mitochondrion localization Source: SGD
    7. mitochondrion inheritance Source: SGD

    Keywords - Ligandi

    Actin-binding, ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29455-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Actin-related protein 2
    Alternative name(s):
    Actin-like protein ARP2
    Short name:
    Actin-like protein 2
    Gene namesi
    Name:ARP2
    Synonyms:ACT2
    Ordered Locus Names:YDL029W
    ORF Names:D2778
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL029w.
    SGDiS000002187. ARP2.

    Subcellular locationi

    Cytoplasmcytoskeletonactin patch 2 Publications

    GO - Cellular componenti

    1. actin cortical patch Source: UniProtKB-SubCell
    2. Arp2/3 protein complex Source: SGD
    3. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 391391Actin-related protein 2PRO_0000089077Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP32381.
    PaxDbiP32381.
    PeptideAtlasiP32381.

    Expressioni

    Gene expression databases

    GenevestigatoriP32381.

    Interactioni

    Subunit structurei

    Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARC18Q059336EBI-2927,EBI-2764
    ARC35P537315EBI-2927,EBI-2770
    ARC40P383284EBI-2927,EBI-2777

    Protein-protein interaction databases

    BioGridi32027. 208 interactions.
    DIPiDIP-2217N.
    IntActiP32381. 26 interactions.
    MINTiMINT-374831.
    STRINGi4932.YDL029W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32381.
    SMRiP32381. Positions 7-387.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the actin family. ARP2 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG5277.
    GeneTreeiENSGT00740000114966.
    HOGENOMiHOG000233340.
    KOiK17260.
    OMAiDYTFNEK.
    OrthoDBiEOG7NPG3Q.

    Family and domain databases

    InterProiIPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    IPR027306. Arp2.
    [Graphical view]
    PANTHERiPTHR11937. PTHR11937. 1 hit.
    PTHR11937:SF37. PTHR11937:SF37. 1 hit.
    PfamiPF00022. Actin. 1 hit.
    [Graphical view]
    PRINTSiPR00190. ACTIN.
    SMARTiSM00268. ACTIN. 1 hit.
    [Graphical view]
    PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32381-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPHNPIVLD QGTGFVKIGR AGENFPDYTF PSIVGRPILR AEERASVATP    50
    LKDIMIGDEA SEVRSYLQIS YPMENGIIKN WTDMELLWDY AFFEQMKLPS 100
    TSNGKILLTE PPMNPLKNRE KMCEVMFEKY DFGGVYVAIQ AVLALYAQGL 150
    SSGVVVDSGD GVTHIVPVYE SVVLSHLTRR LDVAGRDVTR HLIDLLSRRG 200
    YAFNRTADFE TVRQIKEKLC YVSYDLDLDT KLARETTALV ESYELPDGRT 250
    IKVGQERFEA PECLFQPGLV DVEQPGVGEL LFNTVQSADV DIRSSLYKAI 300
    VLSGGSSMYP GLPSRLEKEL KQLWFSRVLH NDPSRLDKFK VRIEDPPRRK 350
    HMVFIGGAVL ASIMADKDHM WLSKQEWQES GPSAMTKFGP R 391
    Length:391
    Mass (Da):44,074
    Last modified:October 1, 1993 - v1
    Checksum:i4C9E8952B477A1B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61502 Genomic DNA. Translation: CAA43718.1.
    Z71781 Genomic DNA. Translation: CAA96460.1.
    Z74077 Genomic DNA. Translation: CAA98588.1.
    BK006938 Genomic DNA. Translation: DAA11823.1.
    PIRiS20225.
    RefSeqiNP_010255.1. NM_001180088.1.

    Genome annotation databases

    EnsemblFungiiYDL029W; YDL029W; YDL029W.
    GeneIDi851532.
    KEGGisce:YDL029W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61502 Genomic DNA. Translation: CAA43718.1 .
    Z71781 Genomic DNA. Translation: CAA96460.1 .
    Z74077 Genomic DNA. Translation: CAA98588.1 .
    BK006938 Genomic DNA. Translation: DAA11823.1 .
    PIRi S20225.
    RefSeqi NP_010255.1. NM_001180088.1.

    3D structure databases

    ProteinModelPortali P32381.
    SMRi P32381. Positions 7-387.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32027. 208 interactions.
    DIPi DIP-2217N.
    IntActi P32381. 26 interactions.
    MINTi MINT-374831.
    STRINGi 4932.YDL029W.

    Proteomic databases

    MaxQBi P32381.
    PaxDbi P32381.
    PeptideAtlasi P32381.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL029W ; YDL029W ; YDL029W .
    GeneIDi 851532.
    KEGGi sce:YDL029W.

    Organism-specific databases

    CYGDi YDL029w.
    SGDi S000002187. ARP2.

    Phylogenomic databases

    eggNOGi COG5277.
    GeneTreei ENSGT00740000114966.
    HOGENOMi HOG000233340.
    KOi K17260.
    OMAi DYTFNEK.
    OrthoDBi EOG7NPG3Q.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29455-MONOMER.

    Miscellaneous databases

    NextBioi 968926.
    PROi P32381.

    Gene expression databases

    Genevestigatori P32381.

    Family and domain databases

    InterProi IPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    IPR027306. Arp2.
    [Graphical view ]
    PANTHERi PTHR11937. PTHR11937. 1 hit.
    PTHR11937:SF37. PTHR11937:SF37. 1 hit.
    Pfami PF00022. Actin. 1 hit.
    [Graphical view ]
    PRINTSi PR00190. ACTIN.
    SMARTi SM00268. ACTIN. 1 hit.
    [Graphical view ]
    PROSITEi PS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "New yeast actin-like gene required late in the cell cycle."
      Schwob E., Martin R.P.
      Nature 355:179-182(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 26109 / X2180.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches."
      Winter D., Podtelejnikov A.V., Mann M., Li R.
      Curr. Biol. 7:519-529(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION.
    5. Erratum
      Winter D., Podtelejnikov A.V., Mann M., Li R.
      Curr. Biol. 7:R593-R593(1997)
    6. "Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast."
      Boldogh I.R., Yang H.C., Nowakowski W.D., Karmon S.L., Hays L.G., Yates J.R. III, Pon L.A.
      Proc. Natl. Acad. Sci. U.S.A. 98:3162-3167(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiARP2_YEAST
    AccessioniPrimary (citable) accession number: P32381
    Secondary accession number(s): D6VRW3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6650 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3