Actin-related protein 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32381 (ARP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Actin-related protein 2

Alternative name(s):
Actin-like protein ARP2
Short name=Actin-like protein 2

Gene names

Name:	ARP2
Synonyms:	ACT2
Ordered Locus Names:	YDL029W
ORF Names:	D2778

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament By similarity.
Subunit structure	Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC. Ref.4
Subcellular location	Cytoplasm › cytoskeleton › actin patch Ref.4 Ref.6.
Miscellaneous	Present with 6650 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the actin family. ARP2 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	ATP-binding Actin-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Arp2/3 complex-mediated actin nucleation Inferred from mutant phenotype PubMed 16862144. Source: SGD CVT pathway Inferred from mutant phenotype PubMed 18287533. Source: SGD ascospore wall assembly Inferred from mutant phenotype PubMed 17118118. Source: SGD establishment of mitochondrion localization Inferred from mutant phenotype PubMed 16107558. Source: SGD mitochondrion inheritance Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	Arp2/3 protein complex Inferred from direct assay PubMed 10377407. Source: SGD actin cortical patch Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay Ref.6. Source: SGD
Molecular_function	ATP binding Inferred from mutant phenotype PubMed 15657399. Source: SGD ATPase activity Inferred from mutant phenotype PubMed 16862144. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
ARC18	Q05933	8	EBI-2927,EBI-2764
ARC35	P53731	8	EBI-2927,EBI-2770
ARC40	P38328	9	EBI-2927,EBI-2777
ARP3	P47117	11	EBI-2927,EBI-2933
LAS17	Q12446	2	EBI-2927,EBI-10022

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 391

391

Actin-related protein 2

PRO_0000089077

Regions

Nucleotide binding

159 – 161

ATP By similarity

Nucleotide binding

213 – 217

ATP By similarity

Nucleotide binding

304 – 309

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphotyrosine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P32381 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 4C9E8952B477A1B9
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FASTA

391

44,074

        10         20         30         40         50         60 
MDPHNPIVLD QGTGFVKIGR AGENFPDYTF PSIVGRPILR AEERASVATP LKDIMIGDEA 

        70         80         90        100        110        120 
SEVRSYLQIS YPMENGIIKN WTDMELLWDY AFFEQMKLPS TSNGKILLTE PPMNPLKNRE 

       130        140        150        160        170        180 
KMCEVMFEKY DFGGVYVAIQ AVLALYAQGL SSGVVVDSGD GVTHIVPVYE SVVLSHLTRR 

       190        200        210        220        230        240 
LDVAGRDVTR HLIDLLSRRG YAFNRTADFE TVRQIKEKLC YVSYDLDLDT KLARETTALV 

       250        260        270        280        290        300 
ESYELPDGRT IKVGQERFEA PECLFQPGLV DVEQPGVGEL LFNTVQSADV DIRSSLYKAI 

       310        320        330        340        350        360 
VLSGGSSMYP GLPSRLEKEL KQLWFSRVLH NDPSRLDKFK VRIEDPPRRK HMVFIGGAVL 

       370        380        390 
ASIMADKDHM WLSKQEWQES GPSAMTKFGP R

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New yeast actin-like gene required late in the cell cycle." Schwob E., Martin R.P. Nature 355:179-182(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26109 / X2180.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches." Winter D., Podtelejnikov A.V., Mann M., Li R. Curr. Biol. 7:519-529(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION.
[5]	Erratum Winter D., Podtelejnikov A.V., Mann M., Li R. Curr. Biol. 7:R593-R593(1997)
[6]	"Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast." Boldogh I.R., Yang H.C., Nowakowski W.D., Karmon S.L., Hays L.G., Yates J.R. III, Pon L.A. Proc. Natl. Acad. Sci. U.S.A. 98:3162-3167(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46 AND TYR-66, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61502 Genomic DNA. Translation: CAA43718.1. Z71781 Genomic DNA. Translation: CAA96460.1. Z74077 Genomic DNA. Translation: CAA98588.1. BK006938 Genomic DNA. Translation: DAA11823.1.
PIR	S20225.
RefSeq	NP_010255.1. NM_001180088.1.
3D structure databases
ProteinModelPortal	P32381.
SMR	P32381. Positions 7-363.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2217N.
IntAct	P32381. 57 interactions.
MINT	MINT-374831.
STRING	4932.YDL029W.
Proteomic databases
PaxDb	P32381.
PeptideAtlas	P32381.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDL029W; YDL029W; YDL029W.
GeneID	851532.
KEGG	sce:YDL029W.
Organism-specific databases
CYGD	YDL029w.
SGD	S000002187. ARP2.
Phylogenomic databases
eggNOG	COG5277.
GeneTree	ENSGT00600000084408.
HOGENOM	HOG000233340.
OMA	FPAHIFP.
OrthoDB	EOG4CZFQF.
Gene expression databases
Genevestigator	P32381.
GermOnline	YDL029W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004000. Actin-related. IPR020902. Actin/actin-like_CS. IPR027306. Arp2. [Graphical view]
PANTHER	PTHR11937. PTHR11937. 1 hit. PTHR11937:SF37. PTHR11937:SF37. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968926.

Entry information

Entry name

ARP2_YEAST

Accession

Primary (citable) accession number: P32381
Secondary accession number(s): D6VRW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents