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P32381 (ARP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Actin-related protein 2
Alternative name(s):
Actin-like protein ARP2
Short name=Actin-like protein 2
Gene names
Name:ARP2
Synonyms:ACT2
Ordered Locus Names:YDL029W
ORF Names:D2778
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament By similarity.

Subunit structure

Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC. Ref.4

Subcellular location

Cytoplasmcytoskeletonactin patch Ref.4 Ref.6.

Miscellaneous

Present with 6650 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the actin family. ARP2 subfamily.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 391391Actin-related protein 2
PRO_0000089077

Regions

Nucleotide binding159 – 1613ATP By similarity
Nucleotide binding213 – 2175ATP By similarity
Nucleotide binding304 – 3096ATP By similarity

Amino acid modifications

Modified residue11N-acetylmethionine Ref.10

Sequences

Sequence LengthMass (Da)Tools
P32381 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 4C9E8952B477A1B9

FASTA39144,074
        10         20         30         40         50         60 
MDPHNPIVLD QGTGFVKIGR AGENFPDYTF PSIVGRPILR AEERASVATP LKDIMIGDEA 

        70         80         90        100        110        120 
SEVRSYLQIS YPMENGIIKN WTDMELLWDY AFFEQMKLPS TSNGKILLTE PPMNPLKNRE 

       130        140        150        160        170        180 
KMCEVMFEKY DFGGVYVAIQ AVLALYAQGL SSGVVVDSGD GVTHIVPVYE SVVLSHLTRR 

       190        200        210        220        230        240 
LDVAGRDVTR HLIDLLSRRG YAFNRTADFE TVRQIKEKLC YVSYDLDLDT KLARETTALV 

       250        260        270        280        290        300 
ESYELPDGRT IKVGQERFEA PECLFQPGLV DVEQPGVGEL LFNTVQSADV DIRSSLYKAI 

       310        320        330        340        350        360 
VLSGGSSMYP GLPSRLEKEL KQLWFSRVLH NDPSRLDKFK VRIEDPPRRK HMVFIGGAVL 

       370        380        390 
ASIMADKDHM WLSKQEWQES GPSAMTKFGP R 

« Hide

References

« Hide 'large scale' references
[1]"New yeast actin-like gene required late in the cell cycle."
Schwob E., Martin R.P.
Nature 355:179-182(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26109 / X2180.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches."
Winter D., Podtelejnikov A.V., Mann M., Li R.
Curr. Biol. 7:519-529(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION.
[5]Erratum
Winter D., Podtelejnikov A.V., Mann M., Li R.
Curr. Biol. 7:R593-R593(1997)
[6]"Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast."
Boldogh I.R., Yang H.C., Nowakowski W.D., Karmon S.L., Hays L.G., Yates J.R. III, Pon L.A.
Proc. Natl. Acad. Sci. U.S.A. 98:3162-3167(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61502 Genomic DNA. Translation: CAA43718.1.
Z71781 Genomic DNA. Translation: CAA96460.1.
Z74077 Genomic DNA. Translation: CAA98588.1.
BK006938 Genomic DNA. Translation: DAA11823.1.
PIRS20225.
RefSeqNP_010255.1. NM_001180088.1.

3D structure databases

ProteinModelPortalP32381.
SMRP32381. Positions 7-387.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32027. 208 interactions.
DIPDIP-2217N.
IntActP32381. 26 interactions.
MINTMINT-374831.
STRING4932.YDL029W.

Proteomic databases

MaxQBP32381.
PaxDbP32381.
PeptideAtlasP32381.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL029W; YDL029W; YDL029W.
GeneID851532.
KEGGsce:YDL029W.

Organism-specific databases

CYGDYDL029w.
SGDS000002187. ARP2.

Phylogenomic databases

eggNOGCOG5277.
GeneTreeENSGT00740000114966.
HOGENOMHOG000233340.
KOK17260.
OMADYTFNEK.
OrthoDBEOG7NPG3Q.

Enzyme and pathway databases

BioCycYEAST:G3O-29455-MONOMER.

Gene expression databases

GenevestigatorP32381.

Family and domain databases

InterProIPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
IPR027306. Arp2.
[Graphical view]
PANTHERPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF37. PTHR11937:SF37. 1 hit.
PfamPF00022. Actin. 1 hit.
[Graphical view]
PRINTSPR00190. ACTIN.
SMARTSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968926.
PROP32381.

Entry information

Entry nameARP2_YEAST
AccessionPrimary (citable) accession number: P32381
Secondary accession number(s): D6VRW3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 11, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families