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P32381

- ARP2_YEAST

UniProt

P32381 - ARP2_YEAST

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Protein

Actin-related protein 2

Gene

ARP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament By similarity.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi159 – 1613ATPBy similarity
Nucleotide bindingi213 – 2175ATPBy similarity
Nucleotide bindingi304 – 3096ATPBy similarity

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. ATP binding Source: SGD

GO - Biological processi

  1. actin filament organization Source: SGD
  2. Arp2/3 complex-mediated actin nucleation Source: SGD
  3. ascospore wall assembly Source: SGD
  4. ATP catabolic process Source: SGD
  5. CVT pathway Source: SGD
  6. establishment of mitochondrion localization Source: SGD
  7. mitochondrion inheritance Source: SGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29455-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 2
Alternative name(s):
Actin-like protein ARP2
Short name:
Actin-like protein 2
Gene namesi
Name:ARP2
Synonyms:ACT2
Ordered Locus Names:YDL029W
ORF Names:D2778
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL029w.
SGDiS000002187. ARP2.

Subcellular locationi

Cytoplasmcytoskeletonactin patch 2 Publications

GO - Cellular componenti

  1. Arp2/3 protein complex Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391Actin-related protein 2PRO_0000089077Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP32381.
PaxDbiP32381.
PeptideAtlasiP32381.

Expressioni

Gene expression databases

GenevestigatoriP32381.

Interactioni

Subunit structurei

Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ARC18Q059336EBI-2927,EBI-2764
ARC35P537315EBI-2927,EBI-2770
ARC40P383284EBI-2927,EBI-2777

Protein-protein interaction databases

BioGridi32027. 209 interactions.
DIPiDIP-2217N.
IntActiP32381. 26 interactions.
MINTiMINT-374831.
STRINGi4932.YDL029W.

Structurei

3D structure databases

ProteinModelPortaliP32381.
SMRiP32381. Positions 7-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP2 subfamily.Curated

Phylogenomic databases

eggNOGiCOG5277.
GeneTreeiENSGT00760000119313.
HOGENOMiHOG000233340.
InParanoidiP32381.
KOiK17260.
OMAiDYTFNEK.
OrthoDBiEOG7NPG3Q.

Family and domain databases

InterProiIPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
IPR027306. Arp2.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF37. PTHR11937:SF37. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32381-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDPHNPIVLD QGTGFVKIGR AGENFPDYTF PSIVGRPILR AEERASVATP
60 70 80 90 100
LKDIMIGDEA SEVRSYLQIS YPMENGIIKN WTDMELLWDY AFFEQMKLPS
110 120 130 140 150
TSNGKILLTE PPMNPLKNRE KMCEVMFEKY DFGGVYVAIQ AVLALYAQGL
160 170 180 190 200
SSGVVVDSGD GVTHIVPVYE SVVLSHLTRR LDVAGRDVTR HLIDLLSRRG
210 220 230 240 250
YAFNRTADFE TVRQIKEKLC YVSYDLDLDT KLARETTALV ESYELPDGRT
260 270 280 290 300
IKVGQERFEA PECLFQPGLV DVEQPGVGEL LFNTVQSADV DIRSSLYKAI
310 320 330 340 350
VLSGGSSMYP GLPSRLEKEL KQLWFSRVLH NDPSRLDKFK VRIEDPPRRK
360 370 380 390
HMVFIGGAVL ASIMADKDHM WLSKQEWQES GPSAMTKFGP R
Length:391
Mass (Da):44,074
Last modified:October 1, 1993 - v1
Checksum:i4C9E8952B477A1B9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61502 Genomic DNA. Translation: CAA43718.1.
Z71781 Genomic DNA. Translation: CAA96460.1.
Z74077 Genomic DNA. Translation: CAA98588.1.
BK006938 Genomic DNA. Translation: DAA11823.1.
PIRiS20225.
RefSeqiNP_010255.1. NM_001180088.1.

Genome annotation databases

EnsemblFungiiYDL029W; YDL029W; YDL029W.
GeneIDi851532.
KEGGisce:YDL029W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61502 Genomic DNA. Translation: CAA43718.1 .
Z71781 Genomic DNA. Translation: CAA96460.1 .
Z74077 Genomic DNA. Translation: CAA98588.1 .
BK006938 Genomic DNA. Translation: DAA11823.1 .
PIRi S20225.
RefSeqi NP_010255.1. NM_001180088.1.

3D structure databases

ProteinModelPortali P32381.
SMRi P32381. Positions 7-387.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32027. 209 interactions.
DIPi DIP-2217N.
IntActi P32381. 26 interactions.
MINTi MINT-374831.
STRINGi 4932.YDL029W.

Proteomic databases

MaxQBi P32381.
PaxDbi P32381.
PeptideAtlasi P32381.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL029W ; YDL029W ; YDL029W .
GeneIDi 851532.
KEGGi sce:YDL029W.

Organism-specific databases

CYGDi YDL029w.
SGDi S000002187. ARP2.

Phylogenomic databases

eggNOGi COG5277.
GeneTreei ENSGT00760000119313.
HOGENOMi HOG000233340.
InParanoidi P32381.
KOi K17260.
OMAi DYTFNEK.
OrthoDBi EOG7NPG3Q.

Enzyme and pathway databases

BioCyci YEAST:G3O-29455-MONOMER.

Miscellaneous databases

NextBioi 968926.
PROi P32381.

Gene expression databases

Genevestigatori P32381.

Family and domain databases

InterProi IPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
IPR027306. Arp2.
[Graphical view ]
PANTHERi PTHR11937. PTHR11937. 1 hit.
PTHR11937:SF37. PTHR11937:SF37. 1 hit.
Pfami PF00022. Actin. 1 hit.
[Graphical view ]
PRINTSi PR00190. ACTIN.
SMARTi SM00268. ACTIN. 1 hit.
[Graphical view ]
PROSITEi PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "New yeast actin-like gene required late in the cell cycle."
    Schwob E., Martin R.P.
    Nature 355:179-182(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 26109 / X2180.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches."
    Winter D., Podtelejnikov A.V., Mann M., Li R.
    Curr. Biol. 7:519-529(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION.
  5. Erratum
    Winter D., Podtelejnikov A.V., Mann M., Li R.
    Curr. Biol. 7:R593-R593(1997)
  6. "Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast."
    Boldogh I.R., Yang H.C., Nowakowski W.D., Karmon S.L., Hays L.G., Yates J.R. III, Pon L.A.
    Proc. Natl. Acad. Sci. U.S.A. 98:3162-3167(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiARP2_YEAST
AccessioniPrimary (citable) accession number: P32381
Secondary accession number(s): D6VRW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6650 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3