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Protein

Actin-related protein 2

Gene

ARP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi159 – 161ATPBy similarity3
Nucleotide bindingi213 – 217ATPBy similarity5
Nucleotide bindingi304 – 309ATPBy similarity6

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: SGD

GO - Biological processi

  • actin filament organization Source: SGD
  • Arp2/3 complex-mediated actin nucleation Source: SGD
  • ascospore wall assembly Source: SGD
  • CVT pathway Source: SGD
  • establishment of mitochondrion localization Source: SGD
  • mitochondrion inheritance Source: SGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29455-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 2
Alternative name(s):
Actin-like protein ARP2
Short name:
Actin-like protein 2
Gene namesi
Name:ARP2
Synonyms:ACT2
Ordered Locus Names:YDL029W
ORF Names:D2778
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL029W.
SGDiS000002187. ARP2.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: UniProtKB-SubCell
  • Arp2/3 protein complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000890771 – 391Actin-related protein 2Add BLAST391

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP32381.
PRIDEiP32381.

PTM databases

iPTMnetiP32381.

Interactioni

Subunit structurei

Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ARC18Q059336EBI-2927,EBI-2764
ARC35P537315EBI-2927,EBI-2770
ARC40P383284EBI-2927,EBI-2777

Protein-protein interaction databases

BioGridi32027. 211 interactors.
DIPiDIP-2217N.
IntActiP32381. 26 interactors.
MINTiMINT-374831.

Structurei

3D structure databases

ProteinModelPortaliP32381.
SMRiP32381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP2 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00840000129873.
HOGENOMiHOG000233340.
InParanoidiP32381.
KOiK17260.
OMAiSTYIAIQ.
OrthoDBiEOG092C2O2N.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR027306. Arp2.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF37. PTHR11937:SF37. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32381-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPHNPIVLD QGTGFVKIGR AGENFPDYTF PSIVGRPILR AEERASVATP
60 70 80 90 100
LKDIMIGDEA SEVRSYLQIS YPMENGIIKN WTDMELLWDY AFFEQMKLPS
110 120 130 140 150
TSNGKILLTE PPMNPLKNRE KMCEVMFEKY DFGGVYVAIQ AVLALYAQGL
160 170 180 190 200
SSGVVVDSGD GVTHIVPVYE SVVLSHLTRR LDVAGRDVTR HLIDLLSRRG
210 220 230 240 250
YAFNRTADFE TVRQIKEKLC YVSYDLDLDT KLARETTALV ESYELPDGRT
260 270 280 290 300
IKVGQERFEA PECLFQPGLV DVEQPGVGEL LFNTVQSADV DIRSSLYKAI
310 320 330 340 350
VLSGGSSMYP GLPSRLEKEL KQLWFSRVLH NDPSRLDKFK VRIEDPPRRK
360 370 380 390
HMVFIGGAVL ASIMADKDHM WLSKQEWQES GPSAMTKFGP R
Length:391
Mass (Da):44,074
Last modified:October 1, 1993 - v1
Checksum:i4C9E8952B477A1B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61502 Genomic DNA. Translation: CAA43718.1.
Z71781 Genomic DNA. Translation: CAA96460.1.
Z74077 Genomic DNA. Translation: CAA98588.1.
BK006938 Genomic DNA. Translation: DAA11823.1.
PIRiS20225.
RefSeqiNP_010255.1. NM_001180088.1.

Genome annotation databases

EnsemblFungiiYDL029W; YDL029W; YDL029W.
GeneIDi851532.
KEGGisce:YDL029W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61502 Genomic DNA. Translation: CAA43718.1.
Z71781 Genomic DNA. Translation: CAA96460.1.
Z74077 Genomic DNA. Translation: CAA98588.1.
BK006938 Genomic DNA. Translation: DAA11823.1.
PIRiS20225.
RefSeqiNP_010255.1. NM_001180088.1.

3D structure databases

ProteinModelPortaliP32381.
SMRiP32381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32027. 211 interactors.
DIPiDIP-2217N.
IntActiP32381. 26 interactors.
MINTiMINT-374831.

PTM databases

iPTMnetiP32381.

Proteomic databases

MaxQBiP32381.
PRIDEiP32381.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL029W; YDL029W; YDL029W.
GeneIDi851532.
KEGGisce:YDL029W.

Organism-specific databases

EuPathDBiFungiDB:YDL029W.
SGDiS000002187. ARP2.

Phylogenomic databases

GeneTreeiENSGT00840000129873.
HOGENOMiHOG000233340.
InParanoidiP32381.
KOiK17260.
OMAiSTYIAIQ.
OrthoDBiEOG092C2O2N.

Enzyme and pathway databases

BioCyciYEAST:G3O-29455-MONOMER.
ReactomeiR-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP32381.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR027306. Arp2.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF37. PTHR11937:SF37. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARP2_YEAST
AccessioniPrimary (citable) accession number: P32381
Secondary accession number(s): D6VRW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6650 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.