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P32375

- ALN_YEAST

UniProt

P32375 - ALN_YEAST

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Protein

Allantoinase

Gene

DAL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Utilization of purines as secondary nitrogen sources, when primary sources are limiting.

Catalytic activityi

(S)-allantoin + H2O = allantoate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn(2+) ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Zinc 1By similarity
Metal bindingi72 – 721Zinc 1By similarity
Metal bindingi157 – 1571Zinc 1; via carbamate groupBy similarity
Metal bindingi157 – 1571Zinc 2; via carbamate groupBy similarity
Metal bindingi193 – 1931Zinc 2By similarity
Metal bindingi250 – 2501Zinc 2By similarity
Metal bindingi323 – 3231Zinc 1By similarity

GO - Molecular functioni

  1. allantoinase activity Source: SGD
  2. cobalt ion binding Source: InterPro
  3. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. allantoin assimilation pathway Source: SGD
  2. purine nucleobase metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Purine metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YIR027C-MONOMER.
YEAST:YIR027C-MONOMER.
ReactomeiREACT_209624. Pyrimidine catabolism.
UniPathwayiUPA00395; UER00653.

Names & Taxonomyi

Protein namesi
Recommended name:
Allantoinase (EC:3.5.2.5)
Gene namesi
Name:DAL1
Ordered Locus Names:YIR027C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIR027c.
SGDiS000001466. DAL1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460AllantoinasePRO_0000165939Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571N6-carboxylysineBy similarity

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two zinc ions.By similarity

Proteomic databases

PaxDbiP32375.

Expressioni

Gene expression databases

GenevestigatoriP32375.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi35018. 23 interactions.
DIPiDIP-4280N.
MINTiMINT-509404.
STRINGi4932.YIR027C.

Structurei

3D structure databases

ProteinModelPortaliP32375.
SMRiP32375. Positions 24-453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DHOase family. Allantoinase subfamily.Curated

Phylogenomic databases

eggNOGiCOG0044.
GeneTreeiENSGT00760000119241.
HOGENOMiHOG000219146.
InParanoidiP32375.
KOiK01466.
OMAiSTHIKEG.
OrthoDBiEOG7K9KC6.

Family and domain databases

Gene3Di2.30.40.10. 2 hits.
InterProiIPR017593. Allantoinase.
IPR006680. Amidohydro_1.
IPR002195. Dihydroorotase_CS.
IPR018228. DNase_TatD-rel_CS.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 1 hit.
TIGRFAMsiTIGR03178. allantoinase. 1 hit.
PROSITEiPS00482. DIHYDROOROTASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32375-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPINAITSDH VIINGANKPA TIVYSTESGT ILDVLEGSVV MEKTEITKYE
60 70 80 90 100
IHTLENVSPC TILPGLVDSH VHLNEPGRTS WEGFETGTQA AISGGVTTVV
110 120 130 140 150
DMPLNAIPPT TNVENFRIKL EAAEGQMWCD VGFWGGLVPH NLPDLIPLVK
160 170 180 190 200
AGVRGFKGFL LDSGVEEFPP IGKEYIEEAL KVLAEEDTMM MFHAELPKAH
210 220 230 240 250
EDQQQPEQSH REYSSFLSSR PDSFEIDAIN LILECLRARN GPVPPVHIVH
260 270 280 290 300
LASMKAIPLI RKARASGLPV TTETCFHYLC IAAEQIPDGA TYFKCCPPIR
310 320 330 340 350
SESNRQGLWD ALREGVIGSV VSDHSPCTPE LKNLQKGDFF DSWGGIASVG
360 370 380 390 400
LGLPLMFTQG CSLVDIVTWC CKNTSHQVGL SHQKGTIAPG YDADLVVFDT
410 420 430 440 450
ASKHKISNSS VYFKNKLTAY NGMTVKGTVL KTILRGQVVY TNANGVSKTP
460
LGQTLLDSRR
Length:460
Mass (Da):50,126
Last modified:February 1, 1995 - v2
Checksum:i887480809B6A8F42
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti438 – 46023VVYTN…LDSRR → WYIRMPTESRKHHWVKLCLI LDVKLKLQIFIKEIL in AAA34553. (PubMed:1803816)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69294 Genomic DNA. Translation: AAA34553.1.
Z38061 Genomic DNA. Translation: CAA86187.1.
BK006942 Genomic DNA. Translation: DAA08574.1.
PIRiS48489.
RefSeqiNP_012293.3. NM_001179549.3.

Genome annotation databases

EnsemblFungiiYIR027C; YIR027C; YIR027C.
GeneIDi854845.
KEGGisce:YIR027C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69294 Genomic DNA. Translation: AAA34553.1 .
Z38061 Genomic DNA. Translation: CAA86187.1 .
BK006942 Genomic DNA. Translation: DAA08574.1 .
PIRi S48489.
RefSeqi NP_012293.3. NM_001179549.3.

3D structure databases

ProteinModelPortali P32375.
SMRi P32375. Positions 24-453.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35018. 23 interactions.
DIPi DIP-4280N.
MINTi MINT-509404.
STRINGi 4932.YIR027C.

Proteomic databases

PaxDbi P32375.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIR027C ; YIR027C ; YIR027C .
GeneIDi 854845.
KEGGi sce:YIR027C.

Organism-specific databases

CYGDi YIR027c.
SGDi S000001466. DAL1.

Phylogenomic databases

eggNOGi COG0044.
GeneTreei ENSGT00760000119241.
HOGENOMi HOG000219146.
InParanoidi P32375.
KOi K01466.
OMAi STHIKEG.
OrthoDBi EOG7K9KC6.

Enzyme and pathway databases

UniPathwayi UPA00395 ; UER00653 .
BioCyci MetaCyc:YIR027C-MONOMER.
YEAST:YIR027C-MONOMER.
Reactomei REACT_209624. Pyrimidine catabolism.

Miscellaneous databases

NextBioi 977734.
PROi P32375.

Gene expression databases

Genevestigatori P32375.

Family and domain databases

Gene3Di 2.30.40.10. 2 hits.
InterProi IPR017593. Allantoinase.
IPR006680. Amidohydro_1.
IPR002195. Dihydroorotase_CS.
IPR018228. DNase_TatD-rel_CS.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view ]
Pfami PF01979. Amidohydro_1. 1 hit.
[Graphical view ]
SUPFAMi SSF51338. SSF51338. 1 hit.
TIGRFAMsi TIGR03178. allantoinase. 1 hit.
PROSITEi PS00482. DIHYDROOROTASE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The allantoinase (DAL1) gene of Saccharomyces cerevisiae."
    Buckholz R.G., Cooper T.G.
    Yeast 7:913-923(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Erratum
    Buckholz R.G., Cooper T.G.
    Yeast 8:239-239(1992)
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiALN_YEAST
AccessioniPrimary (citable) accession number: P32375
Secondary accession number(s): D6VVV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3