Reviewed, UniProtKB/Swiss-Prot P32375 (ALN_YEAST)

Names and origin

Protein names

Recommended name:
    Allantoinase
    EC=3.5.2.5

Gene names

Name:	DAL1
Ordered Locus Names:	YIR027C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Utilization of purines as secondary nitrogen sources, when primary sources are limiting.
Catalytic activity	(S)-allantoin + H2O = allantoate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1.
Subunit structure	Homotetramer By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two zinc ions By similarity.
Sequence similarities	Belongs to the DHOase family. Allantoinase subfamily.

Ontologies

Keywords
Biological process	Purine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	allantoin assimilation pathway Ref.1 Inferred from mutant phenotype. Source: SGD purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	allantoinase activity Ref.1 Inferred from mutant phenotype. Source: SGD endodeoxyribonuclease activity, producing 5'-phosphomonoesters Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Allantoinase		PRO_0000165939
Sites
	Metal binding	70	1	Zinc 1 By similarity
	Metal binding	72	1	Zinc 1 By similarity
	Metal binding	157	1	Zinc 1; via carbamate group By similarity
	Metal binding	157	1	Zinc 2; via carbamate group By similarity
	Metal binding	193	1	Zinc 2 By similarity
	Metal binding	250	1	Zinc 2 By similarity
	Metal binding	323	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	157	1	N6-carboxylysine By similarity
Experimental info
	Sequence conflict	438 – 460	23	VVYTN…LDSRR → WYIRMPTESRKHHWVKLCLI LDVKLKLQIFIKEIL in AAA34553. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32375-1 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 887480809B6A8F42	FASTA	460	50,126
	10 20 30 40 50 60 MPINAITSDH VIINGANKPA TIVYSTESGT ILDVLEGSVV MEKTEITKYE IHTLENVSPC 70 80 90 100 110 120 TILPGLVDSH VHLNEPGRTS WEGFETGTQA AISGGVTTVV DMPLNAIPPT TNVENFRIKL 130 140 150 160 170 180 EAAEGQMWCD VGFWGGLVPH NLPDLIPLVK AGVRGFKGFL LDSGVEEFPP IGKEYIEEAL 190 200 210 220 230 240 KVLAEEDTMM MFHAELPKAH EDQQQPEQSH REYSSFLSSR PDSFEIDAIN LILECLRARN 250 260 270 280 290 300 GPVPPVHIVH LASMKAIPLI RKARASGLPV TTETCFHYLC IAAEQIPDGA TYFKCCPPIR 310 320 330 340 350 360 SESNRQGLWD ALREGVIGSV VSDHSPCTPE LKNLQKGDFF DSWGGIASVG LGLPLMFTQG 370 380 390 400 410 420 CSLVDIVTWC CKNTSHQVGL SHQKGTIAPG YDADLVVFDT ASKHKISNSS VYFKNKLTAY 430 440 450 460 NGMTVKGTVL KTILRGQVVY TNANGVSKTP LGQTLLDSRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The allantoinase (DAL1) gene of Saccharomyces cerevisiae." Buckholz R.G., Cooper T.G. Yeast 7:913-923(1991) [PubMed: 1803816] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erratum Buckholz R.G., Cooper T.G. Yeast 8:239-239(1992)
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M69294 Genomic DNA. Translation: AAA34553.1. Z38061 Genomic DNA. Translation: CAA86187.1.
PIR	S48489.
RefSeq	NP_012293.1.
3D structure databases
SMR	P32375. Positions 56-455.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4280N.
IntAct	P32375. 1 interaction.
STRING	P32375.
Genome annotation databases
Ensembl	YIR027C; YIR027C; YIR027C; Saccharomyces cerevisiae. [Genome view]
GeneID	854845.
KEGG	sce:YIR027C.
NMPDR	fig|4932.3.peg.1836.
Organism-specific databases
CYGD	YIR027c.
SGD	S000001466. DAL1.
Phylogenomic databases
eggNOG	fuNOG07633.
HOGENOM	HBG724623.
OMA	VEEFPAI.
OrthoDB	EOG9NGJ49.
PhylomeDB	P32375.
Enzyme and pathway databases
BioCyc	MetaCyc:YIR027C-MONOMER.
BRENDA	3.5.2.5. 250.
Gene expression databases
ArrayExpress	P32375.
Genevestigator	P32375.
GermOnline	YIR027C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR017593. Allantoinase. IPR006680. Amidohydro_1. IPR002195. Dihydroorotase_CS. IPR018228. DNase_TatD-rel_CS. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR03178. allantoinase. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977734.

Entry information

Entry name

ALN_YEAST

Accession

Primary (citable) accession number: P32375

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 1, 1995
Last modified:	February 9, 2010
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)