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Protein

Allantoinase

Gene

DAL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Utilization of purines as secondary nitrogen sources, when primary sources are limiting.

Catalytic activityi

(S)-allantoin + H2O = allantoate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathway:i(S)-allantoin degradation

This protein is involved in step 1 of the subpathway that synthesizes allantoate from (S)-allantoin.
Proteins known to be involved in this subpathway in this organism are:
  1. Allantoinase (DAL1)
This subpathway is part of the pathway (S)-allantoin degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes allantoate from (S)-allantoin, the pathway (S)-allantoin degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Zinc 1By similarity
Metal bindingi72 – 721Zinc 1By similarity
Metal bindingi157 – 1571Zinc 1; via carbamate groupBy similarity
Metal bindingi157 – 1571Zinc 2; via carbamate groupBy similarity
Metal bindingi193 – 1931Zinc 2By similarity
Metal bindingi250 – 2501Zinc 2By similarity
Metal bindingi323 – 3231Zinc 1By similarity

GO - Molecular functioni

GO - Biological processi

  • allantoin assimilation pathway Source: SGD
  • purine nucleobase metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Purine metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YIR027C-MONOMER.
YEAST:YIR027C-MONOMER.
ReactomeiREACT_299053. Pyrimidine catabolism.
UniPathwayiUPA00395; UER00653.

Names & Taxonomyi

Protein namesi
Recommended name:
Allantoinase (EC:3.5.2.5)
Gene namesi
Name:DAL1
Ordered Locus Names:YIR027C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IX

Organism-specific databases

CYGDiYIR027c.
EuPathDBiFungiDB:YIR027C.
SGDiS000001466. DAL1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460AllantoinasePRO_0000165939Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571N6-carboxylysineBy similarity

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two zinc ions.By similarity

Proteomic databases

PaxDbiP32375.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi35018. 23 interactions.
DIPiDIP-4280N.
MINTiMINT-509404.

Structurei

3D structure databases

ProteinModelPortaliP32375.
SMRiP32375. Positions 24-453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DHOase family. Allantoinase subfamily.Curated

Phylogenomic databases

eggNOGiCOG0044.
GeneTreeiENSGT00760000119241.
HOGENOMiHOG000219146.
InParanoidiP32375.
KOiK01466.
OMAiDITIETC.
OrthoDBiEOG7K9KC6.

Family and domain databases

Gene3Di2.30.40.10. 2 hits.
InterProiIPR017593. Allantoinase.
IPR006680. Amidohydro_1.
IPR002195. Dihydroorotase_CS.
IPR018228. DNase_TatD-rel_CS.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 1 hit.
TIGRFAMsiTIGR03178. allantoinase. 1 hit.
PROSITEiPS00482. DIHYDROOROTASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32375-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPINAITSDH VIINGANKPA TIVYSTESGT ILDVLEGSVV MEKTEITKYE
60 70 80 90 100
IHTLENVSPC TILPGLVDSH VHLNEPGRTS WEGFETGTQA AISGGVTTVV
110 120 130 140 150
DMPLNAIPPT TNVENFRIKL EAAEGQMWCD VGFWGGLVPH NLPDLIPLVK
160 170 180 190 200
AGVRGFKGFL LDSGVEEFPP IGKEYIEEAL KVLAEEDTMM MFHAELPKAH
210 220 230 240 250
EDQQQPEQSH REYSSFLSSR PDSFEIDAIN LILECLRARN GPVPPVHIVH
260 270 280 290 300
LASMKAIPLI RKARASGLPV TTETCFHYLC IAAEQIPDGA TYFKCCPPIR
310 320 330 340 350
SESNRQGLWD ALREGVIGSV VSDHSPCTPE LKNLQKGDFF DSWGGIASVG
360 370 380 390 400
LGLPLMFTQG CSLVDIVTWC CKNTSHQVGL SHQKGTIAPG YDADLVVFDT
410 420 430 440 450
ASKHKISNSS VYFKNKLTAY NGMTVKGTVL KTILRGQVVY TNANGVSKTP
460
LGQTLLDSRR
Length:460
Mass (Da):50,126
Last modified:February 1, 1995 - v2
Checksum:i887480809B6A8F42
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti438 – 46023VVYTN…LDSRR → WYIRMPTESRKHHWVKLCLI LDVKLKLQIFIKEIL in AAA34553 (PubMed:1803816).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69294 Genomic DNA. Translation: AAA34553.1.
Z38061 Genomic DNA. Translation: CAA86187.1.
BK006942 Genomic DNA. Translation: DAA08574.1.
PIRiS48489.
RefSeqiNP_012293.3. NM_001179549.3.

Genome annotation databases

EnsemblFungiiYIR027C; YIR027C; YIR027C.
GeneIDi854845.
KEGGisce:YIR027C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69294 Genomic DNA. Translation: AAA34553.1.
Z38061 Genomic DNA. Translation: CAA86187.1.
BK006942 Genomic DNA. Translation: DAA08574.1.
PIRiS48489.
RefSeqiNP_012293.3. NM_001179549.3.

3D structure databases

ProteinModelPortaliP32375.
SMRiP32375. Positions 24-453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35018. 23 interactions.
DIPiDIP-4280N.
MINTiMINT-509404.

Proteomic databases

PaxDbiP32375.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIR027C; YIR027C; YIR027C.
GeneIDi854845.
KEGGisce:YIR027C.

Organism-specific databases

CYGDiYIR027c.
EuPathDBiFungiDB:YIR027C.
SGDiS000001466. DAL1.

Phylogenomic databases

eggNOGiCOG0044.
GeneTreeiENSGT00760000119241.
HOGENOMiHOG000219146.
InParanoidiP32375.
KOiK01466.
OMAiDITIETC.
OrthoDBiEOG7K9KC6.

Enzyme and pathway databases

UniPathwayiUPA00395; UER00653.
BioCyciMetaCyc:YIR027C-MONOMER.
YEAST:YIR027C-MONOMER.
ReactomeiREACT_299053. Pyrimidine catabolism.

Miscellaneous databases

NextBioi977734.
PROiP32375.

Family and domain databases

Gene3Di2.30.40.10. 2 hits.
InterProiIPR017593. Allantoinase.
IPR006680. Amidohydro_1.
IPR002195. Dihydroorotase_CS.
IPR018228. DNase_TatD-rel_CS.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 1 hit.
TIGRFAMsiTIGR03178. allantoinase. 1 hit.
PROSITEiPS00482. DIHYDROOROTASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The allantoinase (DAL1) gene of Saccharomyces cerevisiae."
    Buckholz R.G., Cooper T.G.
    Yeast 7:913-923(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Erratum
    Buckholz R.G., Cooper T.G.
    Yeast 8:239-239(1992)
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiALN_YEAST
AccessioniPrimary (citable) accession number: P32375
Secondary accession number(s): D6VVV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: July 22, 2015
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.