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P32375 (ALN_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Allantoinase

EC=3.5.2.5
Gene names
Name:DAL1
Ordered Locus Names:YIR027C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Utilization of purines as secondary nitrogen sources, when primary sources are limiting.

Catalytic activity

(S)-allantoin + H2O = allantoate.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Pathway

Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1.

Subunit structure

Homotetramer By similarity.

Post-translational modification

Carbamylation allows a single lysine to coordinate two zinc ions By similarity.

Sequence similarities

Belongs to the DHOase family. Allantoinase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 460460Allantoinase
PRO_0000165939

Sites

Metal binding701Zinc 1 By similarity
Metal binding721Zinc 1 By similarity
Metal binding1571Zinc 1; via carbamate group By similarity
Metal binding1571Zinc 2; via carbamate group By similarity
Metal binding1931Zinc 2 By similarity
Metal binding2501Zinc 2 By similarity
Metal binding3231Zinc 1 By similarity

Amino acid modifications

Modified residue1571N6-carboxylysine By similarity

Experimental info

Sequence conflict438 – 46023VVYTN…LDSRR → WYIRMPTESRKHHWVKLCLI LDVKLKLQIFIKEIL in AAA34553. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32375 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 887480809B6A8F42

FASTA46050,126
        10         20         30         40         50         60 
MPINAITSDH VIINGANKPA TIVYSTESGT ILDVLEGSVV MEKTEITKYE IHTLENVSPC 

        70         80         90        100        110        120 
TILPGLVDSH VHLNEPGRTS WEGFETGTQA AISGGVTTVV DMPLNAIPPT TNVENFRIKL 

       130        140        150        160        170        180 
EAAEGQMWCD VGFWGGLVPH NLPDLIPLVK AGVRGFKGFL LDSGVEEFPP IGKEYIEEAL 

       190        200        210        220        230        240 
KVLAEEDTMM MFHAELPKAH EDQQQPEQSH REYSSFLSSR PDSFEIDAIN LILECLRARN 

       250        260        270        280        290        300 
GPVPPVHIVH LASMKAIPLI RKARASGLPV TTETCFHYLC IAAEQIPDGA TYFKCCPPIR 

       310        320        330        340        350        360 
SESNRQGLWD ALREGVIGSV VSDHSPCTPE LKNLQKGDFF DSWGGIASVG LGLPLMFTQG 

       370        380        390        400        410        420 
CSLVDIVTWC CKNTSHQVGL SHQKGTIAPG YDADLVVFDT ASKHKISNSS VYFKNKLTAY 

       430        440        450        460 
NGMTVKGTVL KTILRGQVVY TNANGVSKTP LGQTLLDSRR 

« Hide

References

« Hide 'large scale' references
[1]"The allantoinase (DAL1) gene of Saccharomyces cerevisiae."
Buckholz R.G., Cooper T.G.
Yeast 7:913-923(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Erratum
Buckholz R.G., Cooper T.G.
Yeast 8:239-239(1992)
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M69294 Genomic DNA. Translation: AAA34553.1.
Z38061 Genomic DNA. Translation: CAA86187.1.
BK006942 Genomic DNA. Translation: DAA08574.1.
PIRS48489.
RefSeqNP_012293.3. NM_001179549.3.

3D structure databases

ProteinModelPortalP32375.
SMRP32375. Positions 7-443.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35018. 23 interactions.
DIPDIP-4280N.
MINTMINT-509404.
STRING4932.YIR027C.

Proteomic databases

PaxDbP32375.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIR027C; YIR027C; YIR027C.
GeneID854845.
KEGGsce:YIR027C.

Organism-specific databases

CYGDYIR027c.
SGDS000001466. DAL1.

Phylogenomic databases

eggNOGCOG0044.
GeneTreeENSGT00740000115468.
HOGENOMHOG000219146.
KOK01466.
OMAAPPIRDS.
OrthoDBEOG7K9KC6.

Enzyme and pathway databases

BioCycMetaCyc:YIR027C-MONOMER.
YEAST:YIR027C-MONOMER.
UniPathwayUPA00395; UER00653.

Gene expression databases

GenevestigatorP32375.

Family and domain databases

Gene3D2.30.40.10. 2 hits.
InterProIPR017593. Allantoinase.
IPR006680. Amidohydro_1.
IPR002195. Dihydroorotase_CS.
IPR018228. DNase_TatD-rel_CS.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMSSF51338. SSF51338. 1 hit.
TIGRFAMsTIGR03178. allantoinase. 1 hit.
PROSITEPS00482. DIHYDROOROTASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977734.
PROP32375.

Entry information

Entry nameALN_YEAST
AccessionPrimary (citable) accession number: P32375
Secondary accession number(s): D6VVV8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways