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P32375

- ALN_YEAST

UniProt

P32375 - ALN_YEAST

Protein

Allantoinase

Gene

DAL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Utilization of purines as secondary nitrogen sources, when primary sources are limiting.

    Catalytic activityi

    (S)-allantoin + H2O = allantoate.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701Zinc 1By similarity
    Metal bindingi72 – 721Zinc 1By similarity
    Metal bindingi157 – 1571Zinc 1; via carbamate groupBy similarity
    Metal bindingi157 – 1571Zinc 2; via carbamate groupBy similarity
    Metal bindingi193 – 1931Zinc 2By similarity
    Metal bindingi250 – 2501Zinc 2By similarity
    Metal bindingi323 – 3231Zinc 1By similarity

    GO - Molecular functioni

    1. allantoinase activity Source: SGD
    2. cobalt ion binding Source: InterPro
    3. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. allantoin assimilation pathway Source: SGD
    2. purine nucleobase metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Purine metabolism

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:YIR027C-MONOMER.
    YEAST:YIR027C-MONOMER.
    UniPathwayiUPA00395; UER00653.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Allantoinase (EC:3.5.2.5)
    Gene namesi
    Name:DAL1
    Ordered Locus Names:YIR027C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIR027c.
    SGDiS000001466. DAL1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 460460AllantoinasePRO_0000165939Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei157 – 1571N6-carboxylysineBy similarity

    Post-translational modificationi

    Carbamylation allows a single lysine to coordinate two zinc ions.By similarity

    Proteomic databases

    PaxDbiP32375.

    Expressioni

    Gene expression databases

    GenevestigatoriP32375.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    BioGridi35018. 23 interactions.
    DIPiDIP-4280N.
    MINTiMINT-509404.
    STRINGi4932.YIR027C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32375.
    SMRiP32375. Positions 11-455.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DHOase family. Allantoinase subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0044.
    GeneTreeiENSGT00740000115468.
    HOGENOMiHOG000219146.
    KOiK01466.
    OMAiSTHIKEG.
    OrthoDBiEOG7K9KC6.

    Family and domain databases

    Gene3Di2.30.40.10. 2 hits.
    InterProiIPR017593. Allantoinase.
    IPR006680. Amidohydro_1.
    IPR002195. Dihydroorotase_CS.
    IPR018228. DNase_TatD-rel_CS.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view]
    PfamiPF01979. Amidohydro_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF51338. SSF51338. 1 hit.
    TIGRFAMsiTIGR03178. allantoinase. 1 hit.
    PROSITEiPS00482. DIHYDROOROTASE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32375-1 [UniParc]FASTAAdd to Basket

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    MPINAITSDH VIINGANKPA TIVYSTESGT ILDVLEGSVV MEKTEITKYE    50
    IHTLENVSPC TILPGLVDSH VHLNEPGRTS WEGFETGTQA AISGGVTTVV 100
    DMPLNAIPPT TNVENFRIKL EAAEGQMWCD VGFWGGLVPH NLPDLIPLVK 150
    AGVRGFKGFL LDSGVEEFPP IGKEYIEEAL KVLAEEDTMM MFHAELPKAH 200
    EDQQQPEQSH REYSSFLSSR PDSFEIDAIN LILECLRARN GPVPPVHIVH 250
    LASMKAIPLI RKARASGLPV TTETCFHYLC IAAEQIPDGA TYFKCCPPIR 300
    SESNRQGLWD ALREGVIGSV VSDHSPCTPE LKNLQKGDFF DSWGGIASVG 350
    LGLPLMFTQG CSLVDIVTWC CKNTSHQVGL SHQKGTIAPG YDADLVVFDT 400
    ASKHKISNSS VYFKNKLTAY NGMTVKGTVL KTILRGQVVY TNANGVSKTP 450
    LGQTLLDSRR 460
    Length:460
    Mass (Da):50,126
    Last modified:February 1, 1995 - v2
    Checksum:i887480809B6A8F42
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti438 – 46023VVYTN…LDSRR → WYIRMPTESRKHHWVKLCLI LDVKLKLQIFIKEIL in AAA34553. (PubMed:1803816)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M69294 Genomic DNA. Translation: AAA34553.1.
    Z38061 Genomic DNA. Translation: CAA86187.1.
    BK006942 Genomic DNA. Translation: DAA08574.1.
    PIRiS48489.
    RefSeqiNP_012293.3. NM_001179549.3.

    Genome annotation databases

    EnsemblFungiiYIR027C; YIR027C; YIR027C.
    GeneIDi854845.
    KEGGisce:YIR027C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M69294 Genomic DNA. Translation: AAA34553.1 .
    Z38061 Genomic DNA. Translation: CAA86187.1 .
    BK006942 Genomic DNA. Translation: DAA08574.1 .
    PIRi S48489.
    RefSeqi NP_012293.3. NM_001179549.3.

    3D structure databases

    ProteinModelPortali P32375.
    SMRi P32375. Positions 11-455.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35018. 23 interactions.
    DIPi DIP-4280N.
    MINTi MINT-509404.
    STRINGi 4932.YIR027C.

    Proteomic databases

    PaxDbi P32375.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIR027C ; YIR027C ; YIR027C .
    GeneIDi 854845.
    KEGGi sce:YIR027C.

    Organism-specific databases

    CYGDi YIR027c.
    SGDi S000001466. DAL1.

    Phylogenomic databases

    eggNOGi COG0044.
    GeneTreei ENSGT00740000115468.
    HOGENOMi HOG000219146.
    KOi K01466.
    OMAi STHIKEG.
    OrthoDBi EOG7K9KC6.

    Enzyme and pathway databases

    UniPathwayi UPA00395 ; UER00653 .
    BioCyci MetaCyc:YIR027C-MONOMER.
    YEAST:YIR027C-MONOMER.

    Miscellaneous databases

    NextBioi 977734.
    PROi P32375.

    Gene expression databases

    Genevestigatori P32375.

    Family and domain databases

    Gene3Di 2.30.40.10. 2 hits.
    InterProi IPR017593. Allantoinase.
    IPR006680. Amidohydro_1.
    IPR002195. Dihydroorotase_CS.
    IPR018228. DNase_TatD-rel_CS.
    IPR011059. Metal-dep_hydrolase_composite.
    [Graphical view ]
    Pfami PF01979. Amidohydro_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51338. SSF51338. 1 hit.
    TIGRFAMsi TIGR03178. allantoinase. 1 hit.
    PROSITEi PS00482. DIHYDROOROTASE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The allantoinase (DAL1) gene of Saccharomyces cerevisiae."
      Buckholz R.G., Cooper T.G.
      Yeast 7:913-923(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Erratum
      Buckholz R.G., Cooper T.G.
      Yeast 8:239-239(1992)
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiALN_YEAST
    AccessioniPrimary (citable) accession number: P32375
    Secondary accession number(s): D6VVV8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3