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Protein

S-M checkpoint control protein rad4

Gene

rad4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component for DNA replication and also the checkpoint control system which couples S and M phases. May directly or indirectly interact with chromatin proteins to form the complex required for the initiation and/or progression of DNA synthesis (PubMed:8343962, PubMed:7957098). Interacts simultaneously with both 'Thr-187' phosphorylation sites in a crb2 dimer for establishing the DNA checkpoint (PubMed:24074952).3 Publications

GO - Molecular functioni

  • protein complex scaffold Source: PomBase

GO - Biological processi

  • DNA replication initiation Source: GO_Central
  • mitotic DNA damage checkpoint Source: PomBase
  • mitotic DNA replication checkpoint Source: PomBase
  • mitotic DNA replication preinitiation complex assembly Source: PomBase
  • mitotic G2 DNA damage checkpoint Source: GO_Central
  • replication fork processing Source: PomBase
Complete GO annotation...

Keywords - Biological processi

DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
S-M checkpoint control protein rad4
Alternative name(s):
P74
Protein cut5
Gene namesi
Name:rad4
Synonyms:cut5
ORF Names:SPAC23C4.18c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23C4.18c.
PomBaseiSPAC23C4.18c. rad4.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • DNA replication preinitiation complex Source: PomBase
  • mitotic spindle Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase
  • site of double-strand break Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000971531 – 648S-M checkpoint control protein rad4Add BLAST648

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei592Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32372.
PRIDEiP32372.

PTM databases

iPTMnetiP32372.

Interactioni

Subunit structurei

Interacts with drc1/sld2 (PubMed:11937031). Interacts (via BRCT1,2 domains) with crb2 (PubMed:9407031, PubMed:14739927); a single rad4 molecule interacts simultaneously with both 'Thr-187' phosphorylation sites in a crb2 dimer (PubMed:24074952).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
crb2P870742EBI-768521,EBI-768448

GO - Molecular functioni

  • protein complex scaffold Source: PomBase

Protein-protein interaction databases

BioGridi279708. 34 interactors.
IntActiP32372. 3 interactors.
MINTiMINT-1179553.

Structurei

Secondary structure

1648
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 9Combined sources4
Beta strandi11 – 16Combined sources6
Helixi19 – 31Combined sources13
Beta strandi38 – 41Combined sources4
Beta strandi46 – 50Combined sources5
Beta strandi52 – 54Combined sources3
Helixi55 – 63Combined sources9
Beta strandi67 – 70Combined sources4
Helixi74 – 83Combined sources10
Turni90 – 96Combined sources7
Turni101 – 104Combined sources4
Beta strandi106 – 111Combined sources6
Helixi116 – 126Combined sources11
Beta strandi133 – 135Combined sources3
Beta strandi141 – 147Combined sources7
Helixi150 – 157Combined sources8
Beta strandi161 – 163Combined sources3
Helixi166 – 174Combined sources9
Helixi180 – 183Combined sources4
Turni302 – 305Combined sources4
Beta strandi307 – 310Combined sources4
Helixi315 – 326Combined sources12
Turni327 – 329Combined sources3
Beta strandi342 – 345Combined sources4
Helixi351 – 353Combined sources3
Beta strandi361 – 363Combined sources3
Helixi364 – 372Combined sources9
Helixi383 – 385Combined sources3
Helixi391 – 393Combined sources3
Helixi394 – 397Combined sources4
Beta strandi401 – 406Combined sources6
Helixi409 – 421Combined sources13
Beta strandi425 – 429Combined sources5
Beta strandi435 – 439Combined sources5
Helixi447 – 458Combined sources12
Beta strandi462 – 465Combined sources4
Helixi467 – 475Combined sources9
Helixi483 – 485Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BMCX-ray1.98A1-186[»]
4BMDX-ray2.50A291-494[»]
4BU0X-ray1.50A1-186[»]
4BU1X-ray2.10A/B1-186[»]
ProteinModelPortaliP32372.
SMRiP32372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 92BRCT 1PROSITE-ProRule annotationAdd BLAST91
Domaini96 – 185BRCT 2PROSITE-ProRule annotationAdd BLAST90
Domaini298 – 384BRCT 3PROSITE-ProRule annotationAdd BLAST87
Domaini392 – 486BRCT 4PROSITE-ProRule annotationAdd BLAST95

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi242 – 249Nuclear localization signalSequence analysis8
Motifi643 – 648Nuclear localization signalSequence analysis6

Sequence similaritiesi

Contains 4 BRCT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiP32372.
KOiK03507.
OMAiVVSAKWL.
OrthoDBiEOG092C0BFA.
PhylomeDBiP32372.

Family and domain databases

CDDicd00027. BRCT. 4 hits.
Gene3Di3.40.50.10190. 4 hits.
InterProiIPR001357. BRCT_dom.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF16589. BRCT_2. 1 hit.
PF12738. PTCB-BRCT. 2 hits.
[Graphical view]
SMARTiSM00292. BRCT. 4 hits.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 4 hits.
PROSITEiPS50172. BRCT. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32372-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSKPLKGF VICCTSIDLK QRTEISTKAT KLGAAYRSDF TKDVTHLIAG
60 70 80 90 100
DFDTPKYKFA AKSRPDIKIM SSEWIPVLYE SWVQGEDLDD GLLVDKHFLP
110 120 130 140 150
TLFKCRVCLT NIGQPERSRI ENYVLKHGGT FCPDLTRDVT HLIAGTSSGR
160 170 180 190 200
KYEYALKWKI NVVCVEWLWQ SIQRNAVLEP QYFQLDMPAE KIGLGAYVRL
210 220 230 240 250
DPNTTEAKSY SENQKISKNK EKSGQSLAAL AEEADLEPVI MKRGKKRDRS
260 270 280 290 300
ILWEELNNGK FEFSSRSEEN SVLLDDFTPE TVQPLEENEL DTELNIENEA
310 320 330 340 350
KLFKNLTFYL YEFPNTKVSR LHKCLSDNGG QISEFLSSTI DFVVIPHYFP
360 370 380 390 400
VDELPIFSFP TVNEWWIERC LYYKKIFGID EHALAKPFFR PSLVPYFNGL
410 420 430 440 450
SIHLTGFKGE ELSHLKKALT ILGAVVHEFL GVQRSILLVN TNEPFSMKTR
460 470 480 490 500
FKIQHATEWN VRVVGVAWLW NIIQSGKFID QVSPWAIDKK ENQEIKKFTN
510 520 530 540 550
QNNMVFPTSD RDTRLQNSLA QQPIGHSTPH NSPSLLSVKK RQNNHIRSNT
560 570 580 590 600
LIQLNSNSKD STIFPRRSVT VPGDKIDTVW KSSVTKPETP TSPQEHVSYI
610 620 630 640
DPDAQREKHK LYAQLTSNVD AIPPANDLQN QENGLLLITE SHRKLRRR
Length:648
Mass (Da):74,168
Last modified:February 1, 1995 - v2
Checksum:iC14EC1EC26863BEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16627 Genomic DNA. Translation: BAA04048.1.
CU329670 Genomic DNA. Translation: CAB16889.1.
X62676 Genomic DNA. Translation: CAA44548.1.
PIRiB40727.
RefSeqiNP_593190.1. NM_001018586.2.

Genome annotation databases

EnsemblFungiiSPAC23C4.18c.1; SPAC23C4.18c.1:pep; SPAC23C4.18c.
GeneIDi2543281.
KEGGispo:SPAC23C4.18c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16627 Genomic DNA. Translation: BAA04048.1.
CU329670 Genomic DNA. Translation: CAB16889.1.
X62676 Genomic DNA. Translation: CAA44548.1.
PIRiB40727.
RefSeqiNP_593190.1. NM_001018586.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BMCX-ray1.98A1-186[»]
4BMDX-ray2.50A291-494[»]
4BU0X-ray1.50A1-186[»]
4BU1X-ray2.10A/B1-186[»]
ProteinModelPortaliP32372.
SMRiP32372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279708. 34 interactors.
IntActiP32372. 3 interactors.
MINTiMINT-1179553.

PTM databases

iPTMnetiP32372.

Proteomic databases

MaxQBiP32372.
PRIDEiP32372.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC23C4.18c.1; SPAC23C4.18c.1:pep; SPAC23C4.18c.
GeneIDi2543281.
KEGGispo:SPAC23C4.18c.

Organism-specific databases

EuPathDBiFungiDB:SPAC23C4.18c.
PomBaseiSPAC23C4.18c. rad4.

Phylogenomic databases

InParanoidiP32372.
KOiK03507.
OMAiVVSAKWL.
OrthoDBiEOG092C0BFA.
PhylomeDBiP32372.

Miscellaneous databases

PROiP32372.

Family and domain databases

CDDicd00027. BRCT. 4 hits.
Gene3Di3.40.50.10190. 4 hits.
InterProiIPR001357. BRCT_dom.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF16589. BRCT_2. 1 hit.
PF12738. PTCB-BRCT. 2 hits.
[Graphical view]
SMARTiSM00292. BRCT. 4 hits.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 4 hits.
PROSITEiPS50172. BRCT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAD4_SCHPO
AccessioniPrimary (citable) accession number: P32372
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.