Phosphoinositide phosphatase SAC1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P32368 (SAC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphoinositide phosphatase SAC1
EC=3.1.3.-

Alternative name(s):
Recessive suppressor of secretory defect

Gene names

Name:	SAC1
Synonyms:	RSD1
Ordered Locus Names:	YKL212W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphoinositide phosphatase that hydrolyzes PtdIns₃P and PtdIns₄P. Has low activity towards PtdIns(3,5)P2. May be involved in the coordination of the activities of the secretory pathway and the actin cytoskeleton. Ref.6 Ref.7
Subunit structure	Component of the SPOTS complex, at least composed of LCB1/2 (LCB1 and/or LCB2), ORM1/2 (ORM1 and/or ORM2), SAC1 and TSC3. Ref.10
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Ref.7.
Miscellaneous	Present with 48000 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 SAC domain.
Caution	It is uncertain whether Met-1 or Met-59 is the initiator.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidylinositol dephosphorylation Inferred from direct assay Ref.6. Source: SGD
Cellular_component	SPOTS complex Inferred from direct assay Ref.10. Source: UniProtKB integral to Golgi membrane Inferred from direct assay PubMed 15657391 PubMed 8314848. Source: SGD integral to endoplasmic reticulum membrane Inferred from direct assay Ref.7 PubMed 15657391 PubMed 8314848. Source: SGD mitochondrial outer membrane Inferred from direct assay PubMed 16407407. Source: SGD
Molecular_function	phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity Inferred from direct assay Ref.6. Source: SGD phosphatidylinositol-3-phosphatase activity Inferred from direct assay Ref.6. Source: SGD phosphatidylinositol-4-phosphate phosphatase activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
OSH3	P38713	2	EBI-16210,EBI-12630

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 623

623

Phosphoinositide phosphatase SAC1

PRO_0000209737

Regions

Topological domain

1 – 521

521

Extracellular Potential

Transmembrane

522 – 544

Helical; Potential

Topological domain

545 – 623

Cytoplasmic Potential

Domain

115 – 454

340

SAC

Compositional bias

507 – 521

Pro-rich

Secondary structure

623

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P32368 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 73DB1FA163BC17AE
Show »

FASTA

623

71,125

        10         20         30         40         50         60 
MTGPIVYVQN ADGIFFKLAE GKGTNDAVIH LANQDQGVRV LGAEEFPVQG EVVKIASLMG 

        70         80         90        100        110        120 
FIKLKLNRYA IIANTVEETG RFNGHVFYRV LQHSIVSTKF NSRIDSEEAE YIKLLELHLK 

       130        140        150        160        170        180 
NSTFYFSYTY DLTNSLQRNE KVGPAASWKT ADERFFWNHY LTEDLRNFAH QDPRIDSFIQ 

       190        200        210        220        230        240 
PVIYGYAKTV DAVLNATPIV LGLITRRSIF RAGTRYFRRG VDKDGNVGNF NETEQILLAE 

       250        260        270        280        290        300 
NPESEKIHVF SFLQTRGSVP IYWAEINNLK YKPNLVLGEN SLDATKKHFD QQKELYGDNY 

       310        320        330        340        350        360 
LVNLVNQKGH ELPVKEGYES VVHALNDPKI HYVYFDFHHE CRKMQWHRVK LLIDHLEKLG 

       370        380        390        400        410        420 
LSNEDFFHKV IDSNGNTVEI VNEQHSVVRT NCMDCLDRTN VVQSVLAQWV LQKEFESADV 

       430        440        450        460        470        480 
VATGSTWEDN APLLTSYQNL WADNADAVSV AYSGTGALKT DFTRTGKRTR LGAFNDFLNS 

       490        500        510        520        530        540 
ASRYYQNNWT DGPRQDSYDL FLGGFRPHTA SIKSPFPDRR PVYIQLIPMI ICAALTVLGA 

       550        560        570        580        590        600 
TIFFPKDRFT SSKNLLYFAG ASIVLALSTK FMFKNGIQFV NWPKLVDVGF LVVHQTHDKE 

       610        620 
QQFKGLKYAQ SPKFSKPDPL KRD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutations in the SAC1 gene suppress defects in yeast Golgi and yeast actin function." Cleves A.E., Novick P.J., Bankaitis V.A. J. Cell Biol. 109:2939-2950(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein." Tzermia M., Horaitis O., Alexandraki D. Yeast 10:663-679(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	Hochstrasser M., Gang G. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-145.
[6]	"SAC1 encodes a regulated lipid phosphoinositide phosphatase, defects in which can be suppressed by the homologous Inp52p and Inp53p phosphatases." Hughes W.E., Woscholski R., Cooke F.T., Patrick R.S., Dove S.K., McDonald N.Q., Parker P.J. J. Biol. Chem. 275:801-808(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Sac1 lipid phosphatase and Stt4 phosphatidylinositol 4-kinase regulate a pool of phosphatidylinositol 4-phosphate that functions in the control of the actin cytoskeleton and vacuole morphology." Foti M., Audhya A., Emr S.D. Mol. Biol. Cell 12:2396-2411(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[10]	"Orm family proteins mediate sphingolipid homeostasis." Breslow D.K., Collins S.R., Bodenmiller B., Aebersold R., Simons K., Shevchenko A., Ejsing C.S., Weissman J.S. Nature 463:1048-1053(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SPOTS COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X51672 Genomic DNA. Translation: CAA35979.1.
X75951 Genomic DNA. Translation: CAA53561.1.
Z28212 Genomic DNA. Translation: CAA82057.1.
U39947 Genomic DNA. Translation: AAA82257.1.
BK006944 Genomic DNA. Translation: DAA08957.1.

PIR

A33622.

RefSeq

NP_012710.1. NM_001179777.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3LWT	X-ray	1.96	X	1-504	[»]

ProteinModelPortal

P32368.

SMR

P32368. Positions 1-456.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6610N.

IntAct

P32368. 20 interactions.

MINT

MINT-1355209.

STRING

4932.YKL212W.

Proteomic databases

PaxDb

P32368.

PeptideAtlas

P32368.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YKL212W; YKL212W; YKL212W.

GeneID

853668.

KEGG

sce:YKL212W.

Organism-specific databases

CYGD

YKL212w.

SGD

S000001695. SAC1.

Phylogenomic databases

eggNOG

COG5329.

GeneTree

ENSGT00530000063393.

HOGENOM

HOG000189112.

OMA

MWRRGAN.

OrthoDB

EOG4HDX2V.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-15189.
YEAST:G3O-31970-MONOMER.
YEAST:MONOMER3O-55.

Gene expression databases

Genevestigator

P32368.

GermOnline

YKL212W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002013. Syja_N.
[Graphical view]

Pfam

PF02383. Syja_N. 1 hit.
[Graphical view]

PROSITE

PS50275. SAC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P32368.

NextBio

974608.

Entry information

Entry name

SAC1_YEAST

Accession

Primary (citable) accession number: P32368
Secondary accession number(s): D6VWZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 29, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents