P32367 (TFC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor tau 95 kDa subunit

Alternative name(s):
TFIIIC 95 kDa subunit
Transcription factor C subunit 1

Gene names

Name:	TFC1
Ordered Locus Names:	YBR123C
ORF Names:	YBR0919

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes. Participates in the interconnection of tauA with tauB via its contacts with TFC3 and TFC6. Serves as a scaffold critical for tauA-DNA spatial configuration and tauB-DNA stability. Ref.7 Ref.9
Subunit structure	Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with TFC3, TFC4 and TFC6. Ref.7 Ref.9
Subcellular location	Nucleus Ref.10.
Miscellaneous	Present with 15800 molecules/cell in log phase SD medium. Ref.11
Sequence similarities	Belongs to the TFIIIC subunit 5 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation from RNA polymerase III promoter Traceable author statement. Source: SGD
Cellular component	transcription factor TFIIIC complex Inferred from direct assay Ref.1Ref.6. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	Transcription factor tau 95 kDa subunit		PRO_0000209717
Regions
	Repeat	221 – 239	19	1
	Repeat	400 – 419	20	2
	Region	221 – 419	199	2 X repeats, Pro-rich
	Motif	296 – 300	5	Nuclear localization signal Potential
	Compositional bias	510 – 620	111	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	617	1	Phosphoserine Ref.12
Experimental info
	Mutagenesis	447	1	E → K: Temperature-sensitive. TFCIII-DNA complexes present a shift in their 5' border, generate slow-migrating TFIIIB-DNA complexes upon stripping TFIIIC by heparin or heat treatment, and allow initiation at downstream sites. TFIIIC-DNA complexes highly unstable at high temperatures. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P32367 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: FD39EB5E2ED4D8C6	FASTA	649	73,554
	10 20 30 40 50 60 MPVEEPLATL SSIPDSSADQ APPLIADEFT LDLPRIPSLE LPLNVSTKHS SIQKAIKMCG 70 80 90 100 110 120 GIEKVKEAFK EHGPIESQHG LQLYLNDDTD SDGSKSYFNE HPVIGKRVPF RDESVILKVT 130 140 150 160 170 180 MPKGTLSKNN NSVKDSIKSL KDSNKLRVTP VSIVDNTIKF REMSDFQIKL DNVPSAREFK 190 200 210 220 230 240 SSFGSLEWNN FKSFVNSVPD NDSQPQENIG NLILDRSVKI PSTDFQLPPP PKLSMVGFPL 250 260 270 280 290 300 LYKYKANPFA KKKKNGVTEV KGTYIKNYQL FVHDLSDKTV IPSQAHEQVL YDFEVAKKTK 310 320 330 340 350 360 VYPGTKSDSK FYESLEECLK ILRELFARRP IWVKRHLDGI VPKKIHHTMK IALALISYRF 370 380 390 400 410 420 TMGPWRNTYI KFGIDPRSSV EYAQYQTEYF KIERKLLSSP IVKKNVPKPP PLVFESDTPG 430 440 450 460 470 480 GIDSRFKFDG KRIPWYLMLQ IDLLIGEPNI AEVFHNVEYL DKANELTGWF KELDLVKIRR 490 500 510 520 530 540 IVKYELGCMV QGNYEYNKYK LKYFKTMLFV KESMVPENKN SEEGMGVNTN KDADGDINMD 550 560 570 580 590 600 AGSQMSSNAI EEDKGIAAGD DFDDNGAITE EPDDAALENE EMDTDQNLKV PASIDDDVDD 610 620 630 640 VDADEEEQES FDVKTASFQD IINKIAKLDP KTAETMKSEL KGFVDEVDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of TFC1, a gene encoding one of two DNA-binding subunits of yeast transcription factor tau (TFIIIC)." Swanson R.N., Conesa C., Lefebvre O., Carles C., Ruet A., Quemeneur E., Gagnon J., Sentenac A. Proc. Natl. Acad. Sci. U.S.A. 88:4887-4891(1991) [PubMed: 2052571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-48; 108-118; 194-201; 246-251; 372-376; 405-422 AND 616-631.
[2]	"Cloning of TFC1, the Saccharomyces cerevisiae gene encoding the 95-kDa subunit of transcription factor TFIIIC." Parsons M.C., Weil P.A. J. Biol. Chem. 267:2894-2901(1992) [PubMed: 1737746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed: 7900426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Purification and characterization of Saccharomyces cerevisiae transcription factor TFIIIC. Polypeptide composition defined with polyclonal antibodies." Parsons M.C., Weil P.A. J. Biol. Chem. 265:5095-5103(1990) [PubMed: 2180956] [Abstract] Cited for: IDENTIFICATION IN TFIIIC.
[7]	"On the subunit composition, stoichiometry, and phosphorylation of the yeast transcription factor TFIIIC/tau." Conesa C., Swanson R.N., Schultz P., Oudet P., Sentenac A. J. Biol. Chem. 268:18047-18052(1993) [PubMed: 7688737] [Abstract] Cited for: FUNCTION, INTERACTION WITH TFC3; TFC4 AND TFC6, PHOSPHORYLATION.
[8]	"Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae." Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H. Genetics 147:435-450(1997) [PubMed: 9335584] [Abstract] Cited for: IDENTIFICATION.
[9]	"The tau95 subunit of yeast TFIIIC influences upstream and downstream functions of TFIIIC.DNA complexes." Jourdain S., Acker J., Ducrot C., Sentenac A., Lefebvre O. J. Biol. Chem. 278:10450-10457(2003) [PubMed: 12533520] [Abstract] Cited for: FUNCTION, INTERACTION WITH TFC3 AND TFC6, MUTAGENESIS OF GLU-447.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-617, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63385 Genomic DNA. Translation: AAA35144.1. M80922 Genomic DNA. Translation: AAA35142.1. X78993 Genomic DNA. Translation: CAA55625.1. Z35992 Genomic DNA. Translation: CAA85080.1. BK006936 Genomic DNA. Translation: DAA07241.1.
PIR	A39711.
RefSeq	NP_009681.1. NM_001178471.1.
3D structure databases
ProteinModelPortal	P32367.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2235N.
IntAct	P32367. 15 interactions.
MINT	MINT-636748.
STRING	P32367.
Proteomic databases
PeptideAtlas	P32367.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR123C; YBR123C; YBR123C.
GeneID	852421.
KEGG	sce:YBR123C.
NMPDR	fig|4932.3.peg.383.
Organism-specific databases
CYGD	YBR123c.
SGD	S000000327. TFC1.
Phylogenomic databases
eggNOG	fuNOG08713.
GeneTree	EFGT00050000002906.
HOGENOM	HBG204166.
OMA	EYFKIER.
OrthoDB	EOG4ZGSMT.
Enzyme and pathway databases
Reactome	REACT_87991. Transcription.
Gene expression databases
ArrayExpress	P32367.
Genevestigator	P32367.
GermOnline	YBR123C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019136. TF_IIIC_su-5. [Graphical view]
KO	K15202.
Pfam	PF09734. Tau95. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971285.

Entry information

Entry name

TFC1_YEAST

Accession

Primary (citable) accession number: P32367
Secondary accession number(s): D6VQC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program