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P32364

- SMY1_YEAST

UniProt

P32364 - SMY1_YEAST

Protein

Kinesin-related protein SMY1

Gene

SMY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Possible microtubule-based motor that can interact or substitute with myosin 2 (MYO2).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi114 – 1218ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. microtubule motor activity Source: InterPro
    3. motor activity Source: SGD

    GO - Biological processi

    1. exocytosis Source: SGD
    2. metabolic process Source: GOC
    3. microtubule-based movement Source: InterPro

    Keywords - Molecular functioni

    Motor protein

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31874-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinesin-related protein SMY1
    Alternative name(s):
    Suppressor protein SMY1
    Gene namesi
    Name:SMY1
    Ordered Locus Names:YKL079W
    ORF Names:YKL409
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL079w.
    SGDiS000001562. SMY1.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. cytoplasm Source: UniProtKB-KW
    4. incipient cellular bud site Source: SGD
    5. kinesin complex Source: InterPro
    6. mating projection tip Source: SGD
    7. microtubule Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 656656Kinesin-related protein SMY1PRO_0000125454Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei583 – 5831Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32364.
    PaxDbiP32364.
    PeptideAtlasiP32364.

    Expressioni

    Gene expression databases

    GenevestigatoriP32364.

    Interactioni

    Protein-protein interaction databases

    BioGridi34053. 152 interactions.
    DIPiDIP-4476N.
    IntActiP32364. 2 interactions.
    MINTiMINT-473438.
    STRINGi4932.YKL079W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32364.
    SMRiP32364. Positions 28-371.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 364338Kinesin motorPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation
    Contains 1 kinesin motor domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5059.
    GeneTreeiENSGT00650000093360.
    OMAiGELETHT.
    OrthoDBiEOG71GB6J.

    Family and domain databases

    Gene3Di3.40.850.10. 1 hit.
    InterProiIPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR24115. PTHR24115. 1 hit.
    PfamiPF00225. Kinesin. 1 hit.
    [Graphical view]
    PRINTSiPR00380. KINESINHEAVY.
    SMARTiSM00129. KISc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32364-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHWNIISKEQ SSSSVSLPTL DSSEPCHIEV ILRAIPEKGL QNNESTFKID    50
    PYENTVLFRT NNPLHETTKE THSTFQFDKV FDANATQEDV QKFLVHPIIN 100
    DVLNGYNGTV ITYGPSFSGK SYSLIGSKES EGILPNICKT LFDTLEKNEE 150
    TKGDSFSVSV LAFEIYMEKT YDLLVPLPER KPLKLHRSSS KMDLEIKDIC 200
    PAHVGSYEDL RSYIQAVQNV GNRMACGDKT ERSRSHLVFQ LHVEQRNRKD 250
    DILKNSSLYL VDLHGAEKFD KRTESTLSQD ALKKLNQSIE ALKNTVRSLS 300
    MKERDSAYSA KGSHSSAYRE SQLTEVLKDS LGGNRKTKVI LTCFLSNVPT 350
    TLSTLEFGDS IRQINNKVTD NTTGLNLKKK MDLFIQDMKI KDDNYVAQIN 400
    ILKAEIDSLK SLHNKSLPED DEKKMLENTK KENIKLKLQL DSITQLLSSS 450
    TNEDPNNRID EEVSEILTKR CEQIAQLELS FDRQMNSNSK LQQELEYKKS 500
    KEEALESMNV RLLEQIQLQE REIQELLTTN AILKGELETH TKLTETRSER 550
    IKSLESSVKE LSLNKSAIPS PRRGSMSSSS GNTMLHIEEG SEISNSPWSA 600
    NTSSKPLVWG ARKVSSSSIA TTGSQESFVA RPFKKGLNLH SIKVTSSTPK 650
    SPSSGS 656
    Length:656
    Mass (Da):73,799
    Last modified:October 1, 1993 - v1
    Checksum:iEC070EADE9EC88BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77143 Genomic DNA. Translation: AAA34441.1.
    M69021 Genomic DNA. Translation: AAA35056.1.
    X75560 Genomic DNA. Translation: CAA53238.1.
    Z28079 Genomic DNA. Translation: CAA81916.1.
    BK006944 Genomic DNA. Translation: DAA09078.1.
    PIRiS25732.
    RefSeqiNP_012844.1. NM_001179645.1.

    Genome annotation databases

    EnsemblFungiiYKL079W; YKL079W; YKL079W.
    GeneIDi853783.
    KEGGisce:YKL079W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77143 Genomic DNA. Translation: AAA34441.1 .
    M69021 Genomic DNA. Translation: AAA35056.1 .
    X75560 Genomic DNA. Translation: CAA53238.1 .
    Z28079 Genomic DNA. Translation: CAA81916.1 .
    BK006944 Genomic DNA. Translation: DAA09078.1 .
    PIRi S25732.
    RefSeqi NP_012844.1. NM_001179645.1.

    3D structure databases

    ProteinModelPortali P32364.
    SMRi P32364. Positions 28-371.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34053. 152 interactions.
    DIPi DIP-4476N.
    IntActi P32364. 2 interactions.
    MINTi MINT-473438.
    STRINGi 4932.YKL079W.

    Proteomic databases

    MaxQBi P32364.
    PaxDbi P32364.
    PeptideAtlasi P32364.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL079W ; YKL079W ; YKL079W .
    GeneIDi 853783.
    KEGGi sce:YKL079W.

    Organism-specific databases

    CYGDi YKL079w.
    SGDi S000001562. SMY1.

    Phylogenomic databases

    eggNOGi COG5059.
    GeneTreei ENSGT00650000093360.
    OMAi GELETHT.
    OrthoDBi EOG71GB6J.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31874-MONOMER.

    Miscellaneous databases

    NextBioi 974903.
    PROi P32364.

    Gene expression databases

    Genevestigatori P32364.

    Family and domain databases

    Gene3Di 3.40.850.10. 1 hit.
    InterProi IPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR24115. PTHR24115. 1 hit.
    Pfami PF00225. Kinesin. 1 hit.
    [Graphical view ]
    PRINTSi PR00380. KINESINHEAVY.
    SMARTi SM00129. KISc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Suppression of a myosin defect by a kinesin-related gene."
      Lillie S.H., Brown S.S.
      Nature 356:358-362(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames."
      James C.M., Gent M.E., Indge K.J., Oliver S.G.
      Yeast 10:247-255(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase."
      Beltran C., Kopecky J., Pan Y.-C.E., Nelson H., Nelson N.
      J. Biol. Chem. 267:774-779(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-185.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-583, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSMY1_YEAST
    AccessioniPrimary (citable) accession number: P32364
    Secondary accession number(s): D6VXK8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1920 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3