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P32364 (SMY1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinesin-related protein SMY1
Alternative name(s):
Suppressor protein SMY1
Gene names
Name:SMY1
Ordered Locus Names:YKL079W
ORF Names:YKL409
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length656 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Possible microtubule-based motor that can interact or substitute with myosin 2 (MYO2).

Subcellular location

Cytoplasmcytoskeleton Probable.

Miscellaneous

Present with 1920 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the kinesin-like protein family.

Contains 1 kinesin-motor domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 656656Kinesin-related protein SMY1
PRO_0000125454

Regions

Domain1 – 370370Kinesin-motor
Nucleotide binding114 – 1218ATP By similarity

Amino acid modifications

Modified residue5831Phosphothreonine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P32364 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: EC070EADE9EC88BC

FASTA65673,799
        10         20         30         40         50         60 
MHWNIISKEQ SSSSVSLPTL DSSEPCHIEV ILRAIPEKGL QNNESTFKID PYENTVLFRT 

        70         80         90        100        110        120 
NNPLHETTKE THSTFQFDKV FDANATQEDV QKFLVHPIIN DVLNGYNGTV ITYGPSFSGK 

       130        140        150        160        170        180 
SYSLIGSKES EGILPNICKT LFDTLEKNEE TKGDSFSVSV LAFEIYMEKT YDLLVPLPER 

       190        200        210        220        230        240 
KPLKLHRSSS KMDLEIKDIC PAHVGSYEDL RSYIQAVQNV GNRMACGDKT ERSRSHLVFQ 

       250        260        270        280        290        300 
LHVEQRNRKD DILKNSSLYL VDLHGAEKFD KRTESTLSQD ALKKLNQSIE ALKNTVRSLS 

       310        320        330        340        350        360 
MKERDSAYSA KGSHSSAYRE SQLTEVLKDS LGGNRKTKVI LTCFLSNVPT TLSTLEFGDS 

       370        380        390        400        410        420 
IRQINNKVTD NTTGLNLKKK MDLFIQDMKI KDDNYVAQIN ILKAEIDSLK SLHNKSLPED 

       430        440        450        460        470        480 
DEKKMLENTK KENIKLKLQL DSITQLLSSS TNEDPNNRID EEVSEILTKR CEQIAQLELS 

       490        500        510        520        530        540 
FDRQMNSNSK LQQELEYKKS KEEALESMNV RLLEQIQLQE REIQELLTTN AILKGELETH 

       550        560        570        580        590        600 
TKLTETRSER IKSLESSVKE LSLNKSAIPS PRRGSMSSSS GNTMLHIEEG SEISNSPWSA 

       610        620        630        640        650 
NTSSKPLVWG ARKVSSSSIA TTGSQESFVA RPFKKGLNLH SIKVTSSTPK SPSSGS 

« Hide

References

« Hide 'large scale' references
[1]"Suppression of a myosin defect by a kinesin-related gene."
Lillie S.H., Brown S.S.
Nature 356:358-362(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames."
James C.M., Gent M.E., Indge K.J., Oliver S.G.
Yeast 10:247-255(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase."
Beltran C., Kopecky J., Pan Y.-C.E., Nelson H., Nelson N.
J. Biol. Chem. 267:774-779(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-185.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-583, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M77143 Genomic DNA. Translation: AAA34441.1.
M69021 Genomic DNA. Translation: AAA35056.1.
X75560 Genomic DNA. Translation: CAA53238.1.
Z28079 Genomic DNA. Translation: CAA81916.1.
BK006944 Genomic DNA. Translation: DAA09078.1.
PIRS25732.
RefSeqNP_012844.1. NM_001179645.1.

3D structure databases

ProteinModelPortalP32364.
SMRP32364. Positions 24-374.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34053. 151 interactions.
DIPDIP-4476N.
IntActP32364. 2 interactions.
MINTMINT-473438.
STRING4932.YKL079W.

Proteomic databases

PaxDbP32364.
PeptideAtlasP32364.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL079W; YKL079W; YKL079W.
GeneID853783.
KEGGsce:YKL079W.

Organism-specific databases

CYGDYKL079w.
SGDS000001562. SMY1.

Phylogenomic databases

eggNOGCOG5059.
GeneTreeENSGT00650000093360.
OMAGELETHT.
OrthoDBEOG71GB6J.

Enzyme and pathway databases

BioCycYEAST:G3O-31874-MONOMER.

Gene expression databases

GenevestigatorP32364.

Family and domain databases

Gene3D3.40.850.10. 1 hit.
InterProIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR24115. PTHR24115. 1 hit.
PfamPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00411. KINESIN_MOTOR_DOMAIN1. 1 hit.
PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974903.
PROP32364.

Entry information

Entry nameSMY1_YEAST
AccessionPrimary (citable) accession number: P32364
Secondary accession number(s): D6VXK8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families