Kinesin-related protein SMY1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P32364 (SMY1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Kinesin-related protein SMY1

Alternative name(s):
Suppressor protein SMY1

Gene names

Name:	SMY1
Ordered Locus Names:	YKL079W
ORF Names:	YKL409

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible microtubule-based motor that can interact or substitute with myosin 2 (MYO2).
Subcellular location	Cytoplasm › cytoskeleton Probable.
Miscellaneous	Present with 1920 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the kinesin-like protein family. Contains 1 kinesin-motor domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	exocytosis Inferred from genetic interaction PubMed 9472039. Source: SGD microtubule-based movement Inferred from electronic annotation. Source: InterPro
Cellular_component	cellular bud neck Inferred from direct assay PubMed 8188749. Source: SGD cellular bud tip Inferred from direct assay PubMed 8188749 PubMed 9472039. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW incipient cellular bud site Inferred from direct assay PubMed 8188749. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807 PubMed 8188749. Source: SGD microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule associated complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule motor activity Inferred from electronic annotation. Source: InterPro motor activity Inferred from sequence or structural similarity PubMed 9472039. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 656

656

Kinesin-related protein SMY1

PRO_0000125454

Regions

Domain

1 – 370

370

Kinesin-motor

Nucleotide binding

114 – 121

ATP By similarity

Amino acid modifications

Modified residue

190

Phosphoserine Ref.7 Ref.8

Modified residue

553

Phosphoserine Ref.8

Modified residue

575

Phosphoserine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P32364 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: EC070EADE9EC88BC
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FASTA

656

73,799

        10         20         30         40         50         60 
MHWNIISKEQ SSSSVSLPTL DSSEPCHIEV ILRAIPEKGL QNNESTFKID PYENTVLFRT 

        70         80         90        100        110        120 
NNPLHETTKE THSTFQFDKV FDANATQEDV QKFLVHPIIN DVLNGYNGTV ITYGPSFSGK 

       130        140        150        160        170        180 
SYSLIGSKES EGILPNICKT LFDTLEKNEE TKGDSFSVSV LAFEIYMEKT YDLLVPLPER 

       190        200        210        220        230        240 
KPLKLHRSSS KMDLEIKDIC PAHVGSYEDL RSYIQAVQNV GNRMACGDKT ERSRSHLVFQ 

       250        260        270        280        290        300 
LHVEQRNRKD DILKNSSLYL VDLHGAEKFD KRTESTLSQD ALKKLNQSIE ALKNTVRSLS 

       310        320        330        340        350        360 
MKERDSAYSA KGSHSSAYRE SQLTEVLKDS LGGNRKTKVI LTCFLSNVPT TLSTLEFGDS 

       370        380        390        400        410        420 
IRQINNKVTD NTTGLNLKKK MDLFIQDMKI KDDNYVAQIN ILKAEIDSLK SLHNKSLPED 

       430        440        450        460        470        480 
DEKKMLENTK KENIKLKLQL DSITQLLSSS TNEDPNNRID EEVSEILTKR CEQIAQLELS 

       490        500        510        520        530        540 
FDRQMNSNSK LQQELEYKKS KEEALESMNV RLLEQIQLQE REIQELLTTN AILKGELETH 

       550        560        570        580        590        600 
TKLTETRSER IKSLESSVKE LSLNKSAIPS PRRGSMSSSS GNTMLHIEEG SEISNSPWSA 

       610        620        630        640        650 
NTSSKPLVWG ARKVSSSSIA TTGSQESFVA RPFKKGLNLH SIKVTSSTPK SPSSGS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Suppression of a myosin defect by a kinesin-related gene." Lillie S.H., Brown S.S. Nature 356:358-362(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames." James C.M., Gent M.E., Indge K.J., Oliver S.G. Yeast 10:247-255(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase." Beltran C., Kopecky J., Pan Y.-C.E., Nelson H., Nelson N. J. Biol. Chem. 267:774-779(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-185.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-190, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-190; SER-553 AND SER-575, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M77143 Genomic DNA. Translation: AAA34441.1. M69021 Genomic DNA. Translation: AAA35056.1. X75560 Genomic DNA. Translation: CAA53238.1. Z28079 Genomic DNA. Translation: CAA81916.1. BK006944 Genomic DNA. Translation: DAA09078.1.
PIR	S25732.
RefSeq	NP_012844.1. NM_001179645.1.
3D structure databases
ProteinModelPortal	P32364.
SMR	P32364. Positions 28-371.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4476N.
IntAct	P32364. 2 interactions.
MINT	MINT-473438.
STRING	4932.YKL079W.
Proteomic databases
PaxDb	P32364.
PeptideAtlas	P32364.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL079W; YKL079W; YKL079W.
GeneID	853783.
KEGG	sce:YKL079W.
Organism-specific databases
CYGD	YKL079w.
SGD	S000001562. SMY1.
Phylogenomic databases
eggNOG	COG5059.
GeneTree	ENSGT00680000099804.
OMA	NIMAVEL.
OrthoDB	EOG4FJCK7.
Gene expression databases
Genevestigator	P32364.
GermOnline	YKL079W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.850.10. 1 hit.
InterPro	IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. [Graphical view]
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974903.

Entry information

Entry name

SMY1_YEAST

Accession

Primary (citable) accession number: P32364
Secondary accession number(s): D6VXK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents