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Protein

Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit

Gene

SPT14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.2 Publications

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.

Enzyme regulationi

Inhibited by Ras, probably via the interaction between RAS2 and ERI1.1 Publication

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

  • phosphatidylinositol N-acetylglucosaminyltransferase activity Source: SGD

GO - Biological processi

  • GPI anchor biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-34070-MONOMER.
UniPathwayiUPA00196.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit (EC:2.4.1.198)
Alternative name(s):
GlcNAc-PI synthesis protein
Gene namesi
Name:SPT14
Synonyms:CWH6, GPI3
Ordered Locus Names:YPL175W
ORF Names:P2269
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL175W.
SGDiS000006096. SPT14.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei407 – 42721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi289 – 2891E → A: Severe growth defect. 1 Publication
Mutagenesisi289 – 2891E → D: Reduces the transferase reaction 12-fold. 1 Publication
Mutagenesisi289 – 2891E → G: Lethal. 1 Publication
Mutagenesisi297 – 2971E → A or G: Lethal. 1 Publication
Mutagenesisi297 – 2971E → D: No transferase activity. 1 Publication
Mutagenesisi301 – 3011C → A: Reduces the transferase reaction 5-fold. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunitPRO_0000080325Add
BLAST

Proteomic databases

MaxQBiP32363.
PRIDEiP32363.

PTM databases

iPTMnetiP32363.

Interactioni

Subunit structurei

Component of the phosphatidylinositol N-acetylglucosaminyltransferase complex composed of at least GPI1, GPI2, GPI3, GPI15, GPI19 and ERI1.

Protein-protein interaction databases

BioGridi36008. 62 interactions.
DIPiDIP-1834N.
IntActiP32363. 1 interaction.
MINTiMINT-385729.

Structurei

3D structure databases

ProteinModelPortaliP32363.
SMRiP32363. Positions 5-379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000014405.
HOGENOMiHOG000203293.
InParanoidiP32363.
KOiK03857.
OMAiREKYQLH.
OrthoDBiEOG092C2VJL.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR013234. PIGA_GPI_anchor_biosynthesis.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF08288. PIGA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32363-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFNIAMLCD FFYPQLGGVE FHIYHLSQKL IDLGHSVVII THAYKDRVGV
60 70 80 90 100
RHLTNGLKVY HVPFFVIFRE TTFPTVFSTF PIIRNILLRE QIQIVHSHGS
110 120 130 140 150
ASTFAHEGIL HANTMGLRTV FTDHSLYGFN NLTSIWVNKL LTFTLTNIDR
160 170 180 190 200
VICVSNTCKE NMIVRTELSP DIISVIPNAV VSEDFKPRDP TGGTKRKQSR
210 220 230 240 250
DKIVIVVIGR LFPNKGSDLL TRIIPKVCSS HEDVEFIVAG DGPKFIDFQQ
260 270 280 290 300
MIESHRLQKR VQLLGSVPHE KVRDVLCQGD IYLHASLTEA FGTILVEAAS
310 320 330 340 350
CNLLIVTTQV GGIPEVLPNE MTVYAEQTSV SDLVQATNKA INIIRSKALD
360 370 380 390 400
TSSFHDSVSK MYDWMDVAKR TVEIYTNISS TSSADDKDWM KMVANLYKRD
410 420 430 440 450
GIWAKHLYLL CGIVEYMLFF LLEWLYPRDE IDLAPKWPKK TVSNETKEAR

ET
Length:452
Mass (Da):51,242
Last modified:June 16, 2009 - v4
Checksum:i7589FD815707EC73
GO

Sequence cautioni

The sequence CAA97882 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63290 Genomic DNA. Translation: CAA44924.1.
Z73531 Genomic DNA. Translation: CAA97882.1. Sequence problems.
BK006949 Genomic DNA. Translation: DAA11259.1.
PIRiS65187.
RefSeqiNP_015150.2. NM_001183989.1.

Genome annotation databases

EnsemblFungiiYPL175W; YPL175W; YPL175W.
GeneIDi855928.
KEGGisce:YPL175W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63290 Genomic DNA. Translation: CAA44924.1.
Z73531 Genomic DNA. Translation: CAA97882.1. Sequence problems.
BK006949 Genomic DNA. Translation: DAA11259.1.
PIRiS65187.
RefSeqiNP_015150.2. NM_001183989.1.

3D structure databases

ProteinModelPortaliP32363.
SMRiP32363. Positions 5-379.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36008. 62 interactions.
DIPiDIP-1834N.
IntActiP32363. 1 interaction.
MINTiMINT-385729.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

PTM databases

iPTMnetiP32363.

Proteomic databases

MaxQBiP32363.
PRIDEiP32363.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL175W; YPL175W; YPL175W.
GeneIDi855928.
KEGGisce:YPL175W.

Organism-specific databases

EuPathDBiFungiDB:YPL175W.
SGDiS000006096. SPT14.

Phylogenomic databases

GeneTreeiENSGT00390000014405.
HOGENOMiHOG000203293.
InParanoidiP32363.
KOiK03857.
OMAiREKYQLH.
OrthoDBiEOG092C2VJL.

Enzyme and pathway databases

UniPathwayiUPA00196.
BioCyciYEAST:G3O-34070-MONOMER.

Miscellaneous databases

PROiP32363.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR013234. PIGA_GPI_anchor_biosynthesis.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF08288. PIGA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPI3_YEAST
AccessioniPrimary (citable) accession number: P32363
Secondary accession number(s): D6W3J3, Q08918
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 16, 2009
Last modified: September 7, 2016
This is version 137 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be involved in transcription.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.