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Protein

Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit

Gene

SPT14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.2 Publications

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be involved in transcription.1 Publication

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.

Enzyme regulationi

Inhibited by Ras, probably via the interaction between RAS2 and ERI1.1 Publication

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

  • phosphatidylinositol N-acetylglucosaminyltransferase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processGPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-34070-MONOMER
UniPathwayiUPA00196

Protein family/group databases

CAZyiGT4 Glycosyltransferase Family 4

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit (EC:2.4.1.198)
Short name:
GPI-GlcNAc transferase complex subunit GPI3
Short name:
GPI-GnT subunit GPI3
Alternative name(s):
GlcNAc-PI synthesis protein
Gene namesi
Name:SPT14
Synonyms:CWH6, GPI3
Ordered Locus Names:YPL175W
ORF Names:P2269
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL175W
SGDiS000006096 SPT14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei407 – 427HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi289E → A: Severe growth defect. 1 Publication1
Mutagenesisi289E → D: Reduces the transferase reaction 12-fold. 1 Publication1
Mutagenesisi289E → G: Lethal. 1 Publication1
Mutagenesisi297E → A or G: Lethal. 1 Publication1
Mutagenesisi297E → D: No transferase activity. 1 Publication1
Mutagenesisi301C → A: Reduces the transferase reaction 5-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000803251 – 452Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunitAdd BLAST452

Proteomic databases

MaxQBiP32363
PaxDbiP32363
PRIDEiP32363

PTM databases

iPTMnetiP32363

Interactioni

Subunit structurei

Component of the phosphatidylinositol N-acetylglucosaminyltransferase (GPI-GlcNAc transferase) complex composed of at least GPI1, GPI2, GPI3, GPI15, GPI19 and ERI1.1 Publication

Protein-protein interaction databases

BioGridi36008, 65 interactors
DIPiDIP-1834N
IntActiP32363, 2 interactors
MINTiP32363
STRINGi4932.YPL175W

Structurei

3D structure databases

ProteinModelPortaliP32363
SMRiP32363
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000014405
HOGENOMiHOG000203293
InParanoidiP32363
KOiK03857
OMAiSHTGREN
OrthoDBiEOG092C2VJL

Family and domain databases

InterProiView protein in InterPro
IPR001296 Glyco_trans_1
IPR013234 PIGA_GPI_anchor_biosynthesis
PfamiView protein in Pfam
PF00534 Glycos_transf_1, 1 hit
PF08288 PIGA, 1 hit

Sequencei

Sequence statusi: Complete.

P32363-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFNIAMLCD FFYPQLGGVE FHIYHLSQKL IDLGHSVVII THAYKDRVGV
60 70 80 90 100
RHLTNGLKVY HVPFFVIFRE TTFPTVFSTF PIIRNILLRE QIQIVHSHGS
110 120 130 140 150
ASTFAHEGIL HANTMGLRTV FTDHSLYGFN NLTSIWVNKL LTFTLTNIDR
160 170 180 190 200
VICVSNTCKE NMIVRTELSP DIISVIPNAV VSEDFKPRDP TGGTKRKQSR
210 220 230 240 250
DKIVIVVIGR LFPNKGSDLL TRIIPKVCSS HEDVEFIVAG DGPKFIDFQQ
260 270 280 290 300
MIESHRLQKR VQLLGSVPHE KVRDVLCQGD IYLHASLTEA FGTILVEAAS
310 320 330 340 350
CNLLIVTTQV GGIPEVLPNE MTVYAEQTSV SDLVQATNKA INIIRSKALD
360 370 380 390 400
TSSFHDSVSK MYDWMDVAKR TVEIYTNISS TSSADDKDWM KMVANLYKRD
410 420 430 440 450
GIWAKHLYLL CGIVEYMLFF LLEWLYPRDE IDLAPKWPKK TVSNETKEAR

ET
Length:452
Mass (Da):51,242
Last modified:June 16, 2009 - v4
Checksum:i7589FD815707EC73
GO

Sequence cautioni

The sequence CAA97882 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63290 Genomic DNA Translation: CAA44924.1
Z73531 Genomic DNA Translation: CAA97882.1 Sequence problems.
BK006949 Genomic DNA Translation: DAA11259.1
PIRiS65187
RefSeqiNP_015150.2, NM_001183989.1

Genome annotation databases

EnsemblFungiiYPL175W; YPL175W; YPL175W
GeneIDi855928
KEGGisce:YPL175W

Similar proteinsi

Entry informationi

Entry nameiGPI3_YEAST
AccessioniPrimary (citable) accession number: P32363
Secondary accession number(s): D6W3J3, Q08918
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 16, 2009
Last modified: March 28, 2018
This is version 151 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health