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P32363 (GPI3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit

EC=2.4.1.198
Alternative name(s):
GlcNAc-PI synthesis protein
Gene names
Name:SPT14
Synonyms:CWH6, GPI3
Ordered Locus Names:YPL175W
ORF Names:P2269
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis. Ref.6 Ref.7

Catalytic activity

UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.

Enzyme regulation

Inhibited by Ras, probably via the interaction between RAS2 and ERI1. Ref.11

Pathway

Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subunit structure

Component of the phosphatidylinositol N-acetylglucosaminyltransferase complex composed of at least GPI1, GPI2, GPI3, GPI15, GPI19 and ERI1.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein Potential.

Miscellaneous

Present with 1480 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

Caution

Was originally (Ref.1) thought to be involved in transcription.

Sequence caution

The sequence CAA97882.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 452452Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit
PRO_0000080325

Regions

Transmembrane407 – 42721Helical; Potential

Experimental info

Mutagenesis2891E → A: Severe growth defect. Ref.9
Mutagenesis2891E → D: Reduces the transferase reaction 12-fold. Ref.9
Mutagenesis2891E → G: Lethal. Ref.9
Mutagenesis2971E → A or G: Lethal. Ref.9
Mutagenesis2971E → D: No transferase activity. Ref.9
Mutagenesis3011C → A: Reduces the transferase reaction 5-fold. Ref.9

Sequences

Sequence LengthMass (Da)Tools
P32363 [UniParc].

Last modified June 16, 2009. Version 4.
Checksum: 7589FD815707EC73

FASTA45251,242
        10         20         30         40         50         60 
MGFNIAMLCD FFYPQLGGVE FHIYHLSQKL IDLGHSVVII THAYKDRVGV RHLTNGLKVY 

        70         80         90        100        110        120 
HVPFFVIFRE TTFPTVFSTF PIIRNILLRE QIQIVHSHGS ASTFAHEGIL HANTMGLRTV 

       130        140        150        160        170        180 
FTDHSLYGFN NLTSIWVNKL LTFTLTNIDR VICVSNTCKE NMIVRTELSP DIISVIPNAV 

       190        200        210        220        230        240 
VSEDFKPRDP TGGTKRKQSR DKIVIVVIGR LFPNKGSDLL TRIIPKVCSS HEDVEFIVAG 

       250        260        270        280        290        300 
DGPKFIDFQQ MIESHRLQKR VQLLGSVPHE KVRDVLCQGD IYLHASLTEA FGTILVEAAS 

       310        320        330        340        350        360 
CNLLIVTTQV GGIPEVLPNE MTVYAEQTSV SDLVQATNKA INIIRSKALD TSSFHDSVSK 

       370        380        390        400        410        420 
MYDWMDVAKR TVEIYTNISS TSSADDKDWM KMVANLYKRD GIWAKHLYLL CGIVEYMLFF 

       430        440        450 
LLEWLYPRDE IDLAPKWPKK TVSNETKEAR ET 

« Hide

References

« Hide 'large scale' references
[1]"The Saccharomyces cerevisiae SPT14 gene is essential for normal expression of the yeast transposon, Ty, as well as for expression of the HIS4 gene and several genes in the mating pathway."
Fassler J.S., Gray W., Lee J.P., Yu G., Gingerich G.
Mol. Gen. Genet. 230:310-320(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Fassler J.S.
Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis."
Schoenbaechler M., Horvath A., Fassler J.S., Riezman H.
EMBO J. 14:1637-1645(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Temperature-sensitive yeast GPI anchoring mutants gpi2 and gpi3 are defective in the synthesis of N-acetylglucosaminyl phosphatidylinositol. Cloning of the GPI2 gene."
Leidich S.D., Kostova Z., Latek R.R., Costello L.C., Drapp D.A., Gray W., Fassler J.S., Orlean P.
J. Biol. Chem. 270:13029-13035(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Photoaffinity labelling with P3-(4-azidoanilido)uridine 5'-triphosphate identifies gpi3p as the UDP-GlcNAc-binding subunit of the enzyme that catalyses formation of GlcNAc-phosphatidylinositol, the first glycolipid intermediate in glycosylphosphatidylinositol synthesis."
Kostova Z., Rancour D.M., Menon A.K., Orlean P.
Biochem. J. 350:815-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBSTRATE BINDING.
[8]"Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
Davis C.A., Grate L., Spingola M., Ares M. Jr.
Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF INTRON.
[9]"Comparative importance in vivo of conserved glutamate residues in the EX7E motif retaining glycosyltransferase Gpi3p, the UDP-GlcNAc-binding subunit of the first enzyme in glycosylphosphatidylinositol assembly."
Kostova Z., Yan B.C., Vainauskas S., Schwartz R., Menon A.K., Orlean P.
Eur. J. Biochem. 270:4507-4514(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF GLU-289; GLU-297 AND CYS-301.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Yeast Ras regulates the complex that catalyzes the first step in GPI-anchor biosynthesis at the ER."
Sobering A.K., Watanabe R., Romeo M.J., Yan B.C., Specht C.A., Orlean P., Riezman H., Levin D.E.
Cell 117:637-648(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63290 Genomic DNA. Translation: CAA44924.1.
Z73531 Genomic DNA. Translation: CAA97882.1. Sequence problems.
BK006949 Genomic DNA. Translation: DAA11259.1.
PIRS65187.
RefSeqNP_015150.2. NM_001183989.1.

3D structure databases

ProteinModelPortalP32363.
SMRP32363. Positions 17-376.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36008. 62 interactions.
DIPDIP-1834N.
IntActP32363. 1 interaction.
MINTMINT-385729.
STRING4932.YPL175W.

Protein family/group databases

CAZyGT4. Glycosyltransferase Family 4.

Proteomic databases

MaxQBP32363.
PaxDbP32363.
PeptideAtlasP32363.
PRIDEP32363.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL175W; YPL175W; YPL175W.
GeneID855928.
KEGGsce:YPL175W.

Organism-specific databases

SGDS000006096. SPT14.

Phylogenomic databases

eggNOGCOG0438.
GeneTreeENSGT00390000014405.
HOGENOMHOG000203293.
KOK03857.
OMAHTGRENT.
OrthoDBEOG7ZGXCR.

Enzyme and pathway databases

BioCycYEAST:G3O-34070-MONOMER.
UniPathwayUPA00196.

Gene expression databases

GenevestigatorP32363.

Family and domain databases

InterProIPR001296. Glyco_trans_1.
IPR013234. PIGA_GPI_anchor_biosynthesis.
[Graphical view]
PfamPF00534. Glycos_transf_1. 1 hit.
PF08288. PIGA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980663.
PROP32363.

Entry information

Entry nameGPI3_YEAST
AccessionPrimary (citable) accession number: P32363
Secondary accession number(s): D6W3J3, Q08918
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 16, 2009
Last modified: June 11, 2014
This is version 122 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways