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P32363

- GPI3_YEAST

UniProt

P32363 - GPI3_YEAST

Protein

Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit

Gene

SPT14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 4 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.2 Publications

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.

    Enzyme regulationi

    Inhibited by Ras, probably via the interaction between RAS2 and ERI1.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. phosphatidylinositol N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    2. UDP-glycosyltransferase activity Source: SGD

    GO - Biological processi

    1. GPI anchor biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    GPI-anchor biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34070-MONOMER.
    UniPathwayiUPA00196.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit (EC:2.4.1.198)
    Alternative name(s):
    GlcNAc-PI synthesis protein
    Gene namesi
    Name:SPT14
    Synonyms:CWH6, GPI3
    Ordered Locus Names:YPL175W
    ORF Names:P2269
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    SGDiS000006096. SPT14.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex Source: SGD
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi289 – 2891E → A: Severe growth defect. 1 Publication
    Mutagenesisi289 – 2891E → D: Reduces the transferase reaction 12-fold. 1 Publication
    Mutagenesisi289 – 2891E → G: Lethal. 1 Publication
    Mutagenesisi297 – 2971E → A or G: Lethal. 1 Publication
    Mutagenesisi297 – 2971E → D: No transferase activity. 1 Publication
    Mutagenesisi301 – 3011C → A: Reduces the transferase reaction 5-fold. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 452452Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunitPRO_0000080325Add
    BLAST

    Proteomic databases

    MaxQBiP32363.
    PaxDbiP32363.
    PeptideAtlasiP32363.
    PRIDEiP32363.

    Expressioni

    Gene expression databases

    GenevestigatoriP32363.

    Interactioni

    Subunit structurei

    Component of the phosphatidylinositol N-acetylglucosaminyltransferase complex composed of at least GPI1, GPI2, GPI3, GPI15, GPI19 and ERI1.

    Protein-protein interaction databases

    BioGridi36008. 62 interactions.
    DIPiDIP-1834N.
    IntActiP32363. 1 interaction.
    MINTiMINT-385729.
    STRINGi4932.YPL175W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32363.
    SMRiP32363. Positions 17-376.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei407 – 42721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0438.
    GeneTreeiENSGT00390000014405.
    HOGENOMiHOG000203293.
    KOiK03857.
    OMAiHTGRENT.
    OrthoDBiEOG7ZGXCR.

    Family and domain databases

    InterProiIPR001296. Glyco_trans_1.
    IPR013234. PIGA_GPI_anchor_biosynthesis.
    [Graphical view]
    PfamiPF00534. Glycos_transf_1. 1 hit.
    PF08288. PIGA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32363-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFNIAMLCD FFYPQLGGVE FHIYHLSQKL IDLGHSVVII THAYKDRVGV    50
    RHLTNGLKVY HVPFFVIFRE TTFPTVFSTF PIIRNILLRE QIQIVHSHGS 100
    ASTFAHEGIL HANTMGLRTV FTDHSLYGFN NLTSIWVNKL LTFTLTNIDR 150
    VICVSNTCKE NMIVRTELSP DIISVIPNAV VSEDFKPRDP TGGTKRKQSR 200
    DKIVIVVIGR LFPNKGSDLL TRIIPKVCSS HEDVEFIVAG DGPKFIDFQQ 250
    MIESHRLQKR VQLLGSVPHE KVRDVLCQGD IYLHASLTEA FGTILVEAAS 300
    CNLLIVTTQV GGIPEVLPNE MTVYAEQTSV SDLVQATNKA INIIRSKALD 350
    TSSFHDSVSK MYDWMDVAKR TVEIYTNISS TSSADDKDWM KMVANLYKRD 400
    GIWAKHLYLL CGIVEYMLFF LLEWLYPRDE IDLAPKWPKK TVSNETKEAR 450
    ET 452
    Length:452
    Mass (Da):51,242
    Last modified:June 16, 2009 - v4
    Checksum:i7589FD815707EC73
    GO

    Sequence cautioni

    The sequence CAA97882.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63290 Genomic DNA. Translation: CAA44924.1.
    Z73531 Genomic DNA. Translation: CAA97882.1. Sequence problems.
    BK006949 Genomic DNA. Translation: DAA11259.1.
    PIRiS65187.
    RefSeqiNP_015150.2. NM_001183989.1.

    Genome annotation databases

    EnsemblFungiiYPL175W; YPL175W; YPL175W.
    GeneIDi855928.
    KEGGisce:YPL175W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63290 Genomic DNA. Translation: CAA44924.1 .
    Z73531 Genomic DNA. Translation: CAA97882.1 . Sequence problems.
    BK006949 Genomic DNA. Translation: DAA11259.1 .
    PIRi S65187.
    RefSeqi NP_015150.2. NM_001183989.1.

    3D structure databases

    ProteinModelPortali P32363.
    SMRi P32363. Positions 17-376.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36008. 62 interactions.
    DIPi DIP-1834N.
    IntActi P32363. 1 interaction.
    MINTi MINT-385729.
    STRINGi 4932.YPL175W.

    Protein family/group databases

    CAZyi GT4. Glycosyltransferase Family 4.

    Proteomic databases

    MaxQBi P32363.
    PaxDbi P32363.
    PeptideAtlasi P32363.
    PRIDEi P32363.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL175W ; YPL175W ; YPL175W .
    GeneIDi 855928.
    KEGGi sce:YPL175W.

    Organism-specific databases

    SGDi S000006096. SPT14.

    Phylogenomic databases

    eggNOGi COG0438.
    GeneTreei ENSGT00390000014405.
    HOGENOMi HOG000203293.
    KOi K03857.
    OMAi HTGRENT.
    OrthoDBi EOG7ZGXCR.

    Enzyme and pathway databases

    UniPathwayi UPA00196 .
    BioCyci YEAST:G3O-34070-MONOMER.

    Miscellaneous databases

    NextBioi 980663.
    PROi P32363.

    Gene expression databases

    Genevestigatori P32363.

    Family and domain databases

    InterProi IPR001296. Glyco_trans_1.
    IPR013234. PIGA_GPI_anchor_biosynthesis.
    [Graphical view ]
    Pfami PF00534. Glycos_transf_1. 1 hit.
    PF08288. PIGA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Saccharomyces cerevisiae SPT14 gene is essential for normal expression of the yeast transposon, Ty, as well as for expression of the HIS4 gene and several genes in the mating pathway."
      Fassler J.S., Gray W., Lee J.P., Yu G., Gingerich G.
      Mol. Gen. Genet. 230:310-320(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Fassler J.S.
      Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The yeast spt14 gene is homologous to the human PIG-A gene and is required for GPI anchor synthesis."
      Schoenbaechler M., Horvath A., Fassler J.S., Riezman H.
      EMBO J. 14:1637-1645(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Temperature-sensitive yeast GPI anchoring mutants gpi2 and gpi3 are defective in the synthesis of N-acetylglucosaminyl phosphatidylinositol. Cloning of the GPI2 gene."
      Leidich S.D., Kostova Z., Latek R.R., Costello L.C., Drapp D.A., Gray W., Fassler J.S., Orlean P.
      J. Biol. Chem. 270:13029-13035(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Photoaffinity labelling with P3-(4-azidoanilido)uridine 5'-triphosphate identifies gpi3p as the UDP-GlcNAc-binding subunit of the enzyme that catalyses formation of GlcNAc-phosphatidylinositol, the first glycolipid intermediate in glycosylphosphatidylinositol synthesis."
      Kostova Z., Rancour D.M., Menon A.K., Orlean P.
      Biochem. J. 350:815-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBSTRATE BINDING.
    8. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
      Davis C.A., Grate L., Spingola M., Ares M. Jr.
      Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF INTRON.
    9. "Comparative importance in vivo of conserved glutamate residues in the EX7E motif retaining glycosyltransferase Gpi3p, the UDP-GlcNAc-binding subunit of the first enzyme in glycosylphosphatidylinositol assembly."
      Kostova Z., Yan B.C., Vainauskas S., Schwartz R., Menon A.K., Orlean P.
      Eur. J. Biochem. 270:4507-4514(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF GLU-289; GLU-297 AND CYS-301.
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Yeast Ras regulates the complex that catalyzes the first step in GPI-anchor biosynthesis at the ER."
      Sobering A.K., Watanabe R., Romeo M.J., Yan B.C., Specht C.A., Orlean P., Riezman H., Levin D.E.
      Cell 117:637-648(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.

    Entry informationi

    Entry nameiGPI3_YEAST
    AccessioniPrimary (citable) accession number: P32363
    Secondary accession number(s): D6W3J3, Q08918
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 123 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1480 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was originally thought to be involved in transcription.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3