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P32361

- IRE1_YEAST

UniProt

P32361 - IRE1_YEAST

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Protein
Serine/threonine-protein kinase/endoribonuclease IRE1
Gene
IRE1, ERN1, YHR079C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices HAC1 precursor mRNA to produce the mature form which then induces transcription of UPR target genes.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.

Enzyme regulationi

The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei702 – 7021ATP
Active sitei797 – 7971Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi680 – 6889ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. endoribonuclease activity Source: SGD
  3. identical protein binding Source: IntAct
  4. metal ion binding Source: UniProtKB-KW
  5. protein binding Source: IntAct
  6. protein homodimerization activity Source: SGD
  7. protein serine/threonine kinase activity Source: SGD
  8. unfolded protein binding Source: SGD

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  2. endoplasmic reticulum unfolded protein response Source: SGD
  3. fungal-type cell wall organization Source: SGD
  4. inositol metabolic process Source: SGD
  5. mRNA processing Source: InterPro
  6. protein homooligomerization Source: SGD
  7. protein phosphorylation Source: SGD
  8. protein trans-autophosphorylation Source: SGD
  9. regulation of transcription, DNA-templated Source: UniProtKB-KW
  10. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation, Unfolded protein response

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31126-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase/endoribonuclease IRE1
Alternative name(s):
Endoplasmic reticulum-to-nucleus signaling 1
Including the following 2 domains:
Gene namesi
Name:IRE1
Synonyms:ERN1
Ordered Locus Names:YHR079C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHR079c.
SGDiS000001121. IRE1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 526508Lumenal Reviewed prediction
Add
BLAST
Transmembranei527 – 55529Helical; Reviewed prediction
Add
BLAST
Topological domaini556 – 1115560Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: Reactome
  2. integral component of endoplasmic reticulum membrane Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi702 – 7021K → A: Loss of autophosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed prediction
Add
BLAST
Chaini19 – 11151097Serine/threonine-protein kinase/endoribonuclease IRE1
PRO_0000024338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi111 – 1111N-linked (GlcNAc...) Reviewed prediction
Glycosylationi213 – 2131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi298 – 2981N-linked (GlcNAc...) Reviewed prediction
Glycosylationi397 – 3971N-linked (GlcNAc...) Reviewed prediction
Modified residuei840 – 8401Phosphoserine; by autocatalysis Inferred
Modified residuei841 – 8411Phosphoserine; by autocatalysis Inferred

Post-translational modificationi

Autophosphorylated mainly on serine residues.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32361.
PRIDEiP32361.

Expressioni

Gene expression databases

GenevestigatoriP32361.

Interactioni

Subunit structurei

Homodimer; in response to the accumulation of unfolded proteins.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-9364,EBI-9364
DCR2Q059242EBI-9364,EBI-3669144

Protein-protein interaction databases

BioGridi36513. 334 interactions.
DIPiDIP-233N.
IntActiP32361. 4 interactions.
MINTiMINT-408165.
STRINGi4932.YHR079C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi116 – 12712
Beta strandi132 – 1365
Turni137 – 1393
Beta strandi142 – 1465
Helixi148 – 1503
Beta strandi154 – 1563
Turni162 – 1643
Beta strandi165 – 1717
Turni175 – 1773
Beta strandi178 – 1836
Turni184 – 1863
Beta strandi187 – 1948
Helixi195 – 1995
Beta strandi203 – 2075
Beta strandi222 – 2265
Beta strandi228 – 23710
Turni238 – 2403
Beta strandi243 – 2486
Beta strandi277 – 28610
Beta strandi300 – 3056
Turni308 – 3103
Helixi311 – 3144
Beta strandi321 – 3233
Beta strandi326 – 3294
Turni330 – 3323
Beta strandi333 – 3375
Beta strandi344 – 3474
Beta strandi355 – 36410
Turni365 – 3684
Beta strandi369 – 3746
Beta strandi390 – 3945
Beta strandi400 – 4045
Turni405 – 4073
Helixi409 – 4135
Helixi419 – 4224
Helixi424 – 4263
Helixi429 – 4335
Helixi435 – 4428
Beta strandi444 – 4463
Turni666 – 6694
Beta strandi674 – 6829
Turni684 – 6863
Beta strandi688 – 70518
Helixi706 – 7083
Helixi709 – 72214
Beta strandi731 – 7366
Beta strandi738 – 7458
Beta strandi749 – 7513
Helixi752 – 7576
Helixi765 – 7695
Helixi774 – 79017
Helixi800 – 8023
Beta strandi803 – 8064
Helixi809 – 8124
Beta strandi823 – 8264
Turni836 – 8383
Helixi851 – 8555
Helixi858 – 8614
Helixi900 – 91415
Turni924 – 9263
Helixi927 – 9337
Helixi947 – 96014
Helixi965 – 9673
Helixi971 – 9755
Helixi978 – 9803
Helixi983 – 99816
Turni1002 – 10054
Helixi1007 – 10137
Helixi1016 – 10194
Helixi1025 – 10284
Helixi1031 – 10355
Beta strandi1038 – 10414
Helixi1048 – 106013
Helixi1062 – 10643
Helixi1067 – 10726
Helixi1078 – 108710
Helixi1091 – 110212
Turni1107 – 11093
Helixi1110 – 11145

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BE1X-ray2.98A/B111-449[»]
2RIOX-ray2.40A/B658-1115[»]
3FBVX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L/M/N641-1115[»]
3LJ0X-ray3.20A/B658-1115[»]
3LJ1X-ray3.33A/B658-1115[»]
3LJ2X-ray3.33A/B658-1115[»]
3SDJX-ray3.65A/B/C/D/E/F/G/H/I/J/K/L/M/N641-1115[»]
3SDMX-ray6.60A/B/C/D/E/F/G641-1115[»]
ProteinModelPortaliP32361.
SMRiP32361. Positions 111-449, 663-1115.

Miscellaneous databases

EvolutionaryTraceiP32361.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini674 – 980307Protein kinase
Add
BLAST
Domaini983 – 1115133KEN
Add
BLAST

Sequence similaritiesi

Contains 1 KEN domain.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00390000015684.
HOGENOMiHOG000213215.
KOiK08852.
OMAiNILWFVE.
OrthoDBiEOG7X3R27.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
InterProiIPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR000719. Prot_kinase_dom.
IPR006567. PUG-dom.
IPR027295. Quinonprotein_ADH-like_fam.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 3 hits.
SM00580. PUG. 1 hit.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32361-1 [UniParc]FASTAAdd to Basket

« Hide

MRLLRRNMLV LTLLVCVFSS IISCSIPLSS RTSRRQIVED EVASTKKLNF     50
NYGVDKNINS PIPAPRTTEG LPNMKLSSYP TPNLLNTADN RRANKKGRRA 100
ANSISVPYLE NRSLNELSLS DILIAADVEG GLHAVDRRNG HIIWSIEPEN 150
FQPLIEIQEP SRLETYETLI IEPFGDGNIY YFNAHQGLQK LPLSIRQLVS 200
TSPLHLKTNI VVNDSGKIVE DEKVYTGSMR TIMYTINMLN GEIISAFGPG 250
SKNGYFGSQS VDCSPEEKIK LQECENMIVI GKTIFELGIH SYDGASYNVT 300
YSTWQQNVLD VPLALQNTFS KDGMCIAPFR DKSLLASDLD FRIARWVSPT 350
FPGIIVGLFD VFNDLRTNEN ILVPHPFNPG DHESISSNKV YLDQTSNLSW 400
FALSSQNFPS LVESAPISRY ASSDRWRVSS IFEDETLFKN AIMGVHQIYN 450
NEYDHLYENY EKTNSLDTTH KYPPLMIDSS VDTTDLHQNN EMNSLKEYMS 500
PEDLEAYRKK IHEQISRELD EKNQNSLLLK FGSLVYRIIE TGVFLLLFLI 550
FCAILQRFKI LPPLYVLLSK IGFMPEKEIP IVESKSLNCP SSSENVTKPF 600
DMKSGKQVVF EGAVNDGSLK SEKDNDDADE DDEKSLDLTT EKKKRKRGSR 650
GGKKGRKSRI ANIPNFEQSL KNLVVSEKIL GYGSSGTVVF QGSFQGRPVA 700
VKRMLIDFCD IALMEIKLLT ESDDHPNVIR YYCSETTDRF LYIALELCNL 750
NLQDLVESKN VSDENLKLQK EYNPISLLRQ IASGVAHLHS LKIIHRDLKP 800
QNILVSTSSR FTADQQTGAE NLRILISDFG LCKKLDSGQS SFRTNLNNPS 850
GTSGWRAPEL LEESNNLQCQ VETEHSSSRH TVVSSDSFYD PFTKRRLTRS 900
IDIFSMGCVF YYILSKGKHP FGDKYSRESN IIRGIFSLDE MKCLHDRSLI 950
AEATDLISQM IDHDPLKRPT AMKVLRHPLF WPKSKKLEFL LKVSDRLEIE 1000
NRDPPSALLM KFDAGSDFVI PSGDWTVKFD KTFMDNLERY RKYHSSKLMD 1050
LLRALRNKYH HFMDLPEDIA ELMGPVPDGF YDYFTKRFPN LLIGVYMIVK 1100
ENLSDDQILR EFLYS 1115
Length:1,115
Mass (Da):126,976
Last modified:February 1, 1995 - v2
Checksum:iBD65D74E74365945
GO

Sequence cautioni

The sequence AAB68894.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti368 – 3681N → S in CAA77763. 1 Publication
Sequence conflicti625 – 6262ND → KH in CAA77763. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11701 Genomic DNA. Translation: CAA77763.1.
L19640 Genomic DNA. Translation: AAA34489.1.
U10556 Genomic DNA. Translation: AAB68894.1. Different initiation.
BK006934 Genomic DNA. Translation: DAA06773.1.
PIRiA47541.
RefSeqiNP_011946.1. NM_001179209.1.

Genome annotation databases

EnsemblFungiiYHR079C; YHR079C; YHR079C.
GeneIDi856478.
KEGGisce:YHR079C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11701 Genomic DNA. Translation: CAA77763.1 .
L19640 Genomic DNA. Translation: AAA34489.1 .
U10556 Genomic DNA. Translation: AAB68894.1 . Different initiation.
BK006934 Genomic DNA. Translation: DAA06773.1 .
PIRi A47541.
RefSeqi NP_011946.1. NM_001179209.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2BE1 X-ray 2.98 A/B 111-449 [» ]
2RIO X-ray 2.40 A/B 658-1115 [» ]
3FBV X-ray 3.20 A/B/C/D/E/F/G/H/I/J/K/L/M/N 641-1115 [» ]
3LJ0 X-ray 3.20 A/B 658-1115 [» ]
3LJ1 X-ray 3.33 A/B 658-1115 [» ]
3LJ2 X-ray 3.33 A/B 658-1115 [» ]
3SDJ X-ray 3.65 A/B/C/D/E/F/G/H/I/J/K/L/M/N 641-1115 [» ]
3SDM X-ray 6.60 A/B/C/D/E/F/G 641-1115 [» ]
ProteinModelPortali P32361.
SMRi P32361. Positions 111-449, 663-1115.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36513. 334 interactions.
DIPi DIP-233N.
IntActi P32361. 4 interactions.
MINTi MINT-408165.
STRINGi 4932.YHR079C.

Proteomic databases

MaxQBi P32361.
PRIDEi P32361.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHR079C ; YHR079C ; YHR079C .
GeneIDi 856478.
KEGGi sce:YHR079C.

Organism-specific databases

CYGDi YHR079c.
SGDi S000001121. IRE1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00390000015684.
HOGENOMi HOG000213215.
KOi K08852.
OMAi NILWFVE.
OrthoDBi EOG7X3R27.

Enzyme and pathway databases

BioCyci YEAST:G3O-31126-MONOMER.
BRENDAi 2.7.11.1. 984.

Miscellaneous databases

EvolutionaryTracei P32361.
NextBioi 982158.
PROi P32361.

Gene expression databases

Genevestigatori P32361.

Family and domain databases

Gene3Di 2.140.10.10. 1 hit.
InterProi IPR010513. KEN_dom.
IPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR000719. Prot_kinase_dom.
IPR006567. PUG-dom.
IPR027295. Quinonprotein_ADH-like_fam.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF06479. Ribonuc_2-5A. 1 hit.
[Graphical view ]
SMARTi SM00564. PQQ. 3 hits.
SM00580. PUG. 1 hit.
[Graphical view ]
SUPFAMi SSF50998. SSF50998. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEi PS51392. KEN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "IRE1 encodes a putative protein kinase containing a membrane-spanning domain and is required for inositol phototrophy in Saccharomyces cerevisiae."
    Nikawa J., Yamashita S.
    Mol. Microbiol. 6:1441-1446(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A transmembrane protein with a cdc2+/CDC28-related kinase activity is required for signaling from the ER to the nucleus."
    Mori K., Ma W., Gething M.J., Sambrook J.
    Cell 74:743-756(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The unfolded protein response pathway in Saccharomyces cerevisiae. Oligomerization and trans-phosphorylation of Ire1p (Ern1p) are required for kinase activation."
    Welihinda A.A., Kaufman R.J.
    J. Biol. Chem. 271:18181-18187(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, HOMODIMERIZATION, MUTAGENESIS OF LYS-702.
  6. "Oligomerization and phosphorylation of the Ire1p kinase during intracellular signaling from the endoplasmic reticulum to the nucleus."
    Shamu C.E., Walter P.
    EMBO J. 15:3028-3039(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, OLIGOMERIZATION, PHOSPHORYLATION.
  7. "The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response."
    Sidrauski C., Walter P.
    Cell 90:1031-1039(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIRE1_YEAST
AccessioniPrimary (citable) accession number: P32361
Secondary accession number(s): D3DL29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3

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