P32359 (TREA_TENMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Trehalase EC=3.2.1.28 Alternative name(s): Alpha,alpha-trehalase Alpha,alpha-trehalose glucohydrolase
Organism	Tenebrio molitor (Yellow mealworm beetle)
Taxonomic identifier	7067 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Tenebrionoidea › Tenebrionidae › Tenebrioninae › Tenebrio

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.
Subcellular location	Secreted. Note: It is incorporated into the wall of the spermatophore.
Tissue specificity	Bean-shaped accessory glands (bags).
Induction	It is increased from 1 to 2 days after adult ecdysis, but decreased after 4 days. It is dependent on ecdysteroid hormone during pupal stage.
Sequence similarities	Belongs to the glycosyl hydrolase 37 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	trehalose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha,alpha-trehalase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 555	539	Trehalase		PRO_0000012059
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
	Glycosylation	334	1	N-linked (GlcNAc...) Potential
	Glycosylation	371	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P32359 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: E7B7C9117A4F074A	FASTA	555	64,464
	10 20 30 40 50 60 MIPFLLMVAF ADTVLQVSAQ SQPSCDSKVY CQGKLLHVVE MSRIFNDSKT FVELKMINDE 70 80 90 100 110 120 QTTLENFDNF LRDTNHKRTR ADLMKFVSDN FKQENEFESW TPTDFTDNPT LLSRIEDKTI 130 140 150 160 170 180 RQFAQDLVKI WPTLARKVKK EVLDYPEHYS LLPVDNGFII PGGRFTEFYY WDSYWIVEGL 190 200 210 220 230 240 LLSDMHETVR GMLDNFLSIV EKYGFIPNGA RVFYLNRSQP PLLTLMVSLY VSATNDMEWL 250 260 270 280 290 300 AKNIRTIDTE LRFWLNNRLV DVVKDGIVYK LAQYNSNSGS PRPESYYEDV TTASVFSDER 310 320 330 340 350 360 DKAELYMDLK SAAESGWDFS SRWIVDEYGG TRGNLSALHT RRIIPVDLNA FLCQAFQKLS 370 380 390 400 410 420 EFYQTLGDYP NATFWSKLVK IWQHSIEMVH YNRDDGIWYD WDNELSQHRR MFFPSNFAPL 430 440 450 460 470 480 WSETFDSRNA EILGEMAAEY FITQNMMDYH GGIPTSLSHT GEQWDYPNAW PPMQSIIVMG 490 500 510 520 530 540 LDKSGSYRAK QLARELARRW VKANLIGFRQ TGEMFEKYNV EVPGQNGGGG EYVVQSGFGW 550 TNGVVLEFIN QFFTT

References

[1]

"Trehalase from male accessory gland of an insect, Tenebrio molitor. cDNA sequencing and developmental profile of the gene expression." Takiguchi M., Niimi T., Su Z.-H., Yaginuma T. Biochem. J. 288:19-22(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Male accessory gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11338 mRNA. Translation: BAA01951.1.
PIR	S27163.
3D structure databases
ProteinModelPortal	P32359.
ModBase	Search...
Protein family/group databases
CAZy	GH37. Glycoside Hydrolase Family 37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR001661. Glyco_hydro_37. IPR018232. Glyco_hydro_37_CS. [Graphical view]
PANTHER	PTHR23403. PTHR23403. 1 hit.
Pfam	PF01204. Trehalase. 1 hit. [Graphical view]
PRINTS	PR00744. GLHYDRLASE37.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00927. TREHALASE_1. 1 hit. PS00928. TREHALASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TREA_TENMO

Accession

Primary (citable) accession number: P32359

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)