P32357 (AAR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A1 cistron-splicing factor AAR2

Gene names

Name:	AAR2
Ordered Locus Names:	YBL074C
ORF Names:	YBL06.06, YBL0611

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the U5 snRNP complex that is required for spliceosome assembly and for pre-mRNA splicing. Involved in splicing pre-mRNA of the A1 cistron and other genes that are important for cell growth. Ref.1 Ref.6
Subunit structure	Heterodimer Probable. Interacts with PRP8 (via RNase H homology domain and MPN domain), competing with BRR2 for the same binding site. Component of a U5 snRNP complex that contains at least the U5 snRNA, PRP8, SNU114, AAR2, SMB1, SMD1, SMD2, SMD3, SME1, SMX2 and SMX3, but is not a component of the U4/U6-U5 tri-snRNP complex. Ref.6 Ref.9 Ref.10
Subcellular location	Cytoplasm. Nucleus. Note: This protein lacks a nuclear localization signal, it may interact in the cytoplasm with a component of spliceosomes via the leucine-zipper and then be transported into the nucleus.
Post-translational modification	Phosphorylated on serine and tyrosine residues. Ref.9
Miscellaneous	Present with 7330 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the AAR2 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	assembly of spliceosomal tri-snRNP Inferred from mutant phenotype Ref.6. Source: SGD
Cellular_component	U5 snRNP Inferred from direct assay Ref.6. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 355

355

A1 cistron-splicing factor AAR2

PRO_0000209705

Regions

Region

261 – 282

22

Leucine-zipper

Compositional bias

332 – 341

10

Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue

253

1

Phosphoserine Ref.9

Modified residue

274

1

Phosphothreonine Ref.9

Modified residue

328

1

Phosphotyrosine Ref.9

Modified residue

331

1

Phosphoserine Ref.8 Ref.9

Modified residue

345

1

Phosphothreonine Ref.9

Experimental info

Mutagenesis

253

1

S → A: No effect on interaction with PRP8. Ref.9

Mutagenesis

253

1

S → D or E: Disrupts interaction with PRP8. Ref.9

Secondary structure

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355

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Beta strand

13 – 17

5

Beta strand

20 – 24

5

Beta strand

32 – 36

5

Beta strand

38 – 40

3

Beta strand

42 – 48

7

Beta strand

51 – 60

10

Helix

62 – 64

3

Beta strand

66 – 72

7

Turn

73 – 76

4

Beta strand

77 – 83

7

Helix

86 – 98

13

Beta strand

102 – 104

3

Helix

113 – 118

6

Helix

123 – 129

7

Beta strand

136 – 141

6

Turn

147 – 149

3

Helix

172 – 174

3

Beta strand

183 – 185

3

Turn

186 – 188

3

Helix

194 – 199

6

Helix

202 – 206

5

Helix

207 – 214

8

Helix

217 – 234

18

Helix

237 – 251

15

Helix

258 – 274

17

Helix

277 – 279

3

Helix

280 – 283

4

Helix

286 – 294

9

Turn

297 – 300

4

Helix

303 – 312

10

Helix

314 – 316

3

Beta strand

345 – 353

9

Sequences

	Sequence		Length	Mass (Da)
	P32357 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 1F030D821D974131	FASTA	355	41,688
	10 20 30 40 50 60 MNTVPFTSAP IEVTIGIDQY SFNVKENQPF HGIKDIPIGH VHVIHFQHAD NSSMRYGYWF 70 80 90 100 110 120 DCRMGNFYIQ YDPKDGLYKM MEERDGAKFE NIVHNFKERQ MMVSYPKIDE DDTWYNLTEF 130 140 150 160 170 180 VQMDKIRKIV RKDENQFSYV DSSMTTVQEN ELLKSSLQKA GSKMEAKNED DPAHSLNYTV 190 200 210 220 230 240 INFKSREAIR PGHEMEDFLD KSYYLNTVML QGIFKNSSNY FGELQFAFLN AMFFGNYGSS 250 260 270 280 290 300 LQWHAMIELI CSSATVPKHM LDKLDEILYY QIKTLPEQYS DILLNERVWN ICLYSSFQKN 310 320 330 340 350 SLHNTEKIME NKYPELLGKD NEDDALIYGI SDEERDDEDD EHNPTIVGGL YYQRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type control of Saccharomyces cerevisiae." Nakazawa N., Harashima S., Oshima Y. Mol. Cell. Biol. 11:5693-5700(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The two genes encoding yeast ribosomal protein S8 reside on different chromosomes, and are closely linked to the hsp70 stress protein genes SSA3 and SSA4." Logghe M., Molemans F., Fiers W., Contreras R. Yeast 10:1093-1100(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces cerevisiae." Boorstein W.R., Craig E.A. Mol. Cell. Biol. 10:3262-3267(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-355.
[6]	"The yeast U5 snRNP coisolated with the U1 snRNP has an unexpected protein composition and includes the splicing factor Aar2p." Gottschalk A., Kastner B., Luhrmann R., Fabrizio P. RNA 7:1554-1565(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-331, MASS SPECTROMETRY.
[9]	"Mechanism for Aar2p function as a U5 snRNP assembly factor." Weber G., Cristao V.F., de Lima Alves F., Santos K.F., Holton N., Rappsilber J., Beggs J.D., Wahl M.C. Genes Dev. 25:1601-1612(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), INTERACTION WITH PRP8, PHOSPHORYLATION AT SER-253; THR-274; TYR-328; SER-331 AND THR-345, MASS SPECTROMETRY, MUTAGENESIS OF SER-253.
[10]	"Crystal structure of Prp8 reveals active site cavity of the spliceosome." Galej W.P., Oubridge C., Newman A.J., Nagai K. Nature 493:638-643(2013) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH PRP8, INTERACTION WITH PRP8.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D90455 Genomic DNA. Translation: BAA14421.1.
Z26879 Genomic DNA. Translation: CAA81524.1.
Z35835 Genomic DNA. Translation: CAA84894.1.
M36115 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07048.1.

PIR

S18510.

RefSeq

NP_009479.1. NM_001178314.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3SBS	X-ray	2.10	A	1-355	[»]
3SBT	X-ray	1.80	B	1-355	[»]
3ZEF	X-ray	3.10	A/D	1-355	[»]
4I43	X-ray	2.00	A	1-355	[»]
4ILG	X-ray	2.10	A	1-355	[»]
4ILI	X-ray	3.20	A/B	1-318	[»]

ProteinModelPortal

P32357.

SMR

P32357. Positions 1-317.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6642N.

IntAct

P32357. 7 interactions.

MINT

MINT-663730.

STRING

4932.YBL074C.

Proteomic databases

PaxDb

P32357.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBL074C; YBL074C; YBL074C.

GeneID

852205.

KEGG

sce:YBL074C.

Organism-specific databases

CYGD

YBL074c.

SGD

S000000170. AAR2.

Phylogenomic databases

eggNOG

NOG331204.

HOGENOM

HOG000033742.

KO

K13205.

OMA

GSSLQWH.

OrthoDB

EOG4J9R8C.

Gene expression databases

Genevestigator

P32357.

GermOnline

YBL074C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR007946. AAR2.
[Graphical view]

PANTHER

PTHR12689. PTHR12689. 1 hit.

Pfam

PF05282. AAR2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

970703.

Entry information

Entry name

AAR2_YEAST

Accession

Primary (citable) accession number: P32357
Secondary accession number(s): D6VPS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program