Reviewed, UniProtKB/Swiss-Prot P32357 (AAR2_YEAST)

Names and origin

Protein names

Recommended name:
    A1 cistron-splicing factor AAR2

Gene names

Name:	AAR2
Ordered Locus Names:	YBL074C
ORF Names:	YBL0611, YBL06.06

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in splicing pre-mRNA of the A1 cistron and other genes that are important for cell growth.
Subunit structure	Heterodimer Probable.
Subcellular location	Cytoplasm. Nucleus. Note: This protein lacks a nuclear localization signal, it may interact in the cytoplasm with a component of spliceosomes via the leucine-zipper and then be transported into the nucleus.
Miscellaneous	Present with 7330 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the AAR2 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	assembly of spliceosomal tri-snRNP Inferred from mutant phenotype. Source: SGD
Cellular component	U5 snRNP Inferred from direct assay. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	A1 cistron-splicing factor AAR2		PRO_0000209705
Regions
	Domain	261 – 282	22	Leucine-zipper
	Compositional bias	332 – 341	10	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	331	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P32357-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 1F030D821D974131	FASTA	355	41,688
	10 20 30 40 50 60 MNTVPFTSAP IEVTIGIDQY SFNVKENQPF HGIKDIPIGH VHVIHFQHAD NSSMRYGYWF 70 80 90 100 110 120 DCRMGNFYIQ YDPKDGLYKM MEERDGAKFE NIVHNFKERQ MMVSYPKIDE DDTWYNLTEF 130 140 150 160 170 180 VQMDKIRKIV RKDENQFSYV DSSMTTVQEN ELLKSSLQKA GSKMEAKNED DPAHSLNYTV 190 200 210 220 230 240 INFKSREAIR PGHEMEDFLD KSYYLNTVML QGIFKNSSNY FGELQFAFLN AMFFGNYGSS 250 260 270 280 290 300 LQWHAMIELI CSSATVPKHM LDKLDEILYY QIKTLPEQYS DILLNERVWN ICLYSSFQKN 310 320 330 340 350 SLHNTEKIME NKYPELLGKD NEDDALIYGI SDEERDDEDD EHNPTIVGGL YYQRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type control of Saccharomyces cerevisiae." Nakazawa N., Harashima S., Oshima Y. Mol. Cell. Biol. 11:5693-5700(1991) [PubMed: 1922071] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The two genes encoding yeast ribosomal protein S8 reside on different chromosomes, and are closely linked to the hsp70 stress protein genes SSA3 and SSA4." Logghe M., Molemans F., Fiers W., Contreras R. Yeast 10:1093-1100(1994) [PubMed: 7992509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces cerevisiae." Boorstein W.R., Craig E.A. Mol. Cell. Biol. 10:3262-3267(1990) [PubMed: 2188113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-355.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-331, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D90455 Genomic DNA. Translation: BAA14421.1. Z26879 Genomic DNA. Translation: CAA81524.1. Z35835 Genomic DNA. Translation: CAA84894.1. M36115 Genomic DNA. No translation available.
PIR	S18510.
RefSeq	NP_009479.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6642N.
IntAct	P32357. 138 interactions.
STRING	P32357.
Genome annotation databases
Ensembl	YBL074C; YBL074C; YBL074C; Saccharomyces cerevisiae. [Genome view]
GeneID	852205.
KEGG	sce:YBL074C.
NMPDR	fig|4932.3.peg.165.
Organism-specific databases
CYGD	YBL074c.
SGD	S000000170. AAR2.
Phylogenomic databases
HOGENOM	P32357.
OMA	GSSLQWH
OrthoDB	EOG9N5XCX
Gene expression databases
ArrayExpress	P32357.
Genevestigator	P32357.
GermOnline	YBL074C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007946. AAR2. [Graphical view]
PANTHER	PTHR12689. AAR2. 1 hit.
Pfam	PF05282. AAR2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970703.

Entry information

Entry name

AAR2_YEAST

Accession

Primary (citable) accession number: P32357

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 24, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)