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P32357 (AAR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
A1 cistron-splicing factor AAR2
Gene names
Name:AAR2
Ordered Locus Names:YBL074C
ORF Names:YBL06.06, YBL0611
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the U5 snRNP complex that is required for spliceosome assembly and for pre-mRNA splicing. Involved in splicing pre-mRNA of the A1 cistron and other genes that are important for cell growth. Ref.1 Ref.6

Subunit structure

Heterodimer Probable. Interacts with PRP8 (via RNase H homology domain and MPN domain), competing with BRR2 for the same binding site. Component of a U5 snRNP complex that contains at least the U5 snRNA, PRP8, SNU114, AAR2, SMB1, SMD1, SMD2, SMD3, SME1, SMX2 and SMX3, but is not a component of the U4/U6-U5 tri-snRNP complex. Ref.6 Ref.9 Ref.10

Subcellular location

Cytoplasm. Nucleus. Note: This protein lacks a nuclear localization signal, it may interact in the cytoplasm with a component of spliceosomes via the leucine-zipper and then be transported into the nucleus.

Post-translational modification

Phosphorylated on serine and tyrosine residues. Ref.9

Miscellaneous

Present with 7330 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the AAR2 family.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentCytoplasm
Nucleus
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processspliceosomal tri-snRNP complex assembly

Inferred from mutant phenotype Ref.6. Source: SGD

   Cellular_componentU5 snRNP

Inferred from direct assay Ref.6. Source: SGD

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355A1 cistron-splicing factor AAR2
PRO_0000209705

Regions

Region261 – 28222Leucine-zipper
Compositional bias332 – 34110Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue2531Phosphoserine Ref.9
Modified residue2741Phosphothreonine Ref.9
Modified residue3281Phosphotyrosine Ref.9
Modified residue3311Phosphoserine Ref.9
Modified residue3451Phosphothreonine Ref.9

Experimental info

Mutagenesis2531S → A: No effect on interaction with PRP8. Ref.9
Mutagenesis2531S → D or E: Disrupts interaction with PRP8. Ref.9

Secondary structure

................................................................ 355
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32357 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 1F030D821D974131

FASTA35541,688
        10         20         30         40         50         60 
MNTVPFTSAP IEVTIGIDQY SFNVKENQPF HGIKDIPIGH VHVIHFQHAD NSSMRYGYWF 

        70         80         90        100        110        120 
DCRMGNFYIQ YDPKDGLYKM MEERDGAKFE NIVHNFKERQ MMVSYPKIDE DDTWYNLTEF 

       130        140        150        160        170        180 
VQMDKIRKIV RKDENQFSYV DSSMTTVQEN ELLKSSLQKA GSKMEAKNED DPAHSLNYTV 

       190        200        210        220        230        240 
INFKSREAIR PGHEMEDFLD KSYYLNTVML QGIFKNSSNY FGELQFAFLN AMFFGNYGSS 

       250        260        270        280        290        300 
LQWHAMIELI CSSATVPKHM LDKLDEILYY QIKTLPEQYS DILLNERVWN ICLYSSFQKN 

       310        320        330        340        350 
SLHNTEKIME NKYPELLGKD NEDDALIYGI SDEERDDEDD EHNPTIVGGL YYQRP 

« Hide

References

« Hide 'large scale' references
[1]"AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type control of Saccharomyces cerevisiae."
Nakazawa N., Harashima S., Oshima Y.
Mol. Cell. Biol. 11:5693-5700(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"The two genes encoding yeast ribosomal protein S8 reside on different chromosomes, and are closely linked to the hsp70 stress protein genes SSA3 and SSA4."
Logghe M., Molemans F., Fiers W., Contreras R.
Yeast 10:1093-1100(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces cerevisiae."
Boorstein W.R., Craig E.A.
Mol. Cell. Biol. 10:3262-3267(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 77-355.
[6]"The yeast U5 snRNP coisolated with the U1 snRNP has an unexpected protein composition and includes the splicing factor Aar2p."
Gottschalk A., Kastner B., Luhrmann R., Fabrizio P.
RNA 7:1554-1565(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Mechanism for Aar2p function as a U5 snRNP assembly factor."
Weber G., Cristao V.F., de Lima Alves F., Santos K.F., Holton N., Rappsilber J., Beggs J.D., Wahl M.C.
Genes Dev. 25:1601-1612(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), INTERACTION WITH PRP8, PHOSPHORYLATION AT SER-253; THR-274; TYR-328; SER-331 AND THR-345, MASS SPECTROMETRY, MUTAGENESIS OF SER-253.
[10]"Crystal structure of Prp8 reveals active site cavity of the spliceosome."
Galej W.P., Oubridge C., Newman A.J., Nagai K.
Nature 493:638-643(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH PRP8, INTERACTION WITH PRP8.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D90455 Genomic DNA. Translation: BAA14421.1.
Z26879 Genomic DNA. Translation: CAA81524.1.
Z35835 Genomic DNA. Translation: CAA84894.1.
M36115 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07048.1.
PIRS18510.
RefSeqNP_009479.1. NM_001178314.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3SBSX-ray2.10A1-355[»]
3SBTX-ray1.80B1-355[»]
3ZEFX-ray3.10A/D1-355[»]
4I43X-ray2.00A1-355[»]
4ILGX-ray2.10A1-355[»]
4ILHX-ray1.85B1-331[»]
4ILIX-ray3.20A/B1-318[»]
ProteinModelPortalP32357.
SMRP32357. Positions 1-355.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32628. 20 interactions.
DIPDIP-6642N.
IntActP32357. 7 interactions.
MINTMINT-663730.
STRING4932.YBL074C.

Proteomic databases

PaxDbP32357.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL074C; YBL074C; YBL074C.
GeneID852205.
KEGGsce:YBL074C.

Organism-specific databases

CYGDYBL074c.
SGDS000000170. AAR2.

Phylogenomic databases

eggNOGNOG331204.
HOGENOMHOG000033742.
KOK13205.
OMAYLDKSWY.
OrthoDBEOG7GTTGG.

Enzyme and pathway databases

BioCycYEAST:G3O-28966-MONOMER.

Gene expression databases

GenevestigatorP32357.

Family and domain databases

InterProIPR007946. AAR2.
[Graphical view]
PANTHERPTHR12689. PTHR12689. 1 hit.
PfamPF05282. AAR2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970703.

Entry information

Entry nameAAR2_YEAST
AccessionPrimary (citable) accession number: P32357
Secondary accession number(s): D6VPS8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: February 19, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references