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Protein

A1 cistron-splicing factor AAR2

Gene

AAR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the U5 snRNP complex that is required for spliceosome assembly and for pre-mRNA splicing. Involved in splicing pre-mRNA of the A1 cistron and other genes that are important for cell growth.2 Publications

GO - Biological processi

  • spliceosomal tri-snRNP complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-28966-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
A1 cistron-splicing factor AAR2
Gene namesi
Name:AAR2
Ordered Locus Names:YBL074C
ORF Names:YBL06.06, YBL0611
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL074C.
SGDiS000000170. AAR2.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: This protein lacks a nuclear localization signal, it may interact in the cytoplasm with a component of spliceosomes via the leucine-zipper and then be transported into the nucleus.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • U5 snRNP Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi253S → A: No effect on interaction with PRP8. 1 Publication1
Mutagenesisi253S → D or E: Disrupts interaction with PRP8. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002097051 – 355A1 cistron-splicing factor AAR2Add BLAST355

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253Phosphoserine1 Publication1
Modified residuei274Phosphothreonine1 Publication1
Modified residuei328Phosphotyrosine1 Publication1
Modified residuei331Phosphoserine1 Publication1
Modified residuei345Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated on serine and tyrosine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32357.
PRIDEiP32357.

PTM databases

iPTMnetiP32357.

Interactioni

Subunit structurei

Heterodimer (Probable). Interacts with PRP8 (via RNase H homology domain and MPN domain), competing with BRR2 for the same binding site. Component of a U5 snRNP complex that contains at least the U5 snRNA, PRP8, SNU114, AAR2, SMB1, SMD1, SMD2, SMD3, SME1, SMX2 and SMX3, but is not a component of the U4/U6-U5 tri-snRNP complex.Curated3 Publications

Protein-protein interaction databases

BioGridi32628. 17 interactors.
DIPiDIP-6642N.
IntActiP32357. 8 interactors.
MINTiMINT-663730.

Structurei

Secondary structure

1355
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Beta strandi13 – 17Combined sources5
Beta strandi20 – 24Combined sources5
Beta strandi32 – 36Combined sources5
Beta strandi38 – 40Combined sources3
Beta strandi42 – 48Combined sources7
Beta strandi51 – 60Combined sources10
Helixi62 – 64Combined sources3
Beta strandi66 – 72Combined sources7
Turni73 – 76Combined sources4
Beta strandi77 – 83Combined sources7
Helixi86 – 98Combined sources13
Beta strandi102 – 104Combined sources3
Helixi113 – 118Combined sources6
Helixi123 – 129Combined sources7
Beta strandi136 – 141Combined sources6
Turni147 – 149Combined sources3
Beta strandi153 – 155Combined sources3
Helixi172 – 174Combined sources3
Beta strandi183 – 185Combined sources3
Turni186 – 188Combined sources3
Helixi194 – 199Combined sources6
Helixi202 – 206Combined sources5
Helixi207 – 214Combined sources8
Helixi217 – 234Combined sources18
Helixi237 – 251Combined sources15
Helixi258 – 274Combined sources17
Helixi277 – 279Combined sources3
Helixi280 – 283Combined sources4
Helixi286 – 294Combined sources9
Turni297 – 300Combined sources4
Helixi303 – 312Combined sources10
Helixi314 – 316Combined sources3
Beta strandi345 – 353Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SBSX-ray2.10A1-355[»]
3SBTX-ray1.80B1-355[»]
3ZEFX-ray3.10A/D1-355[»]
4I43X-ray2.00A1-355[»]
4ILGX-ray2.10A1-355[»]
4ILHX-ray1.85B1-331[»]
4ILIX-ray3.20A/B1-318[»]
ProteinModelPortaliP32357.
SMRiP32357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni261 – 282Leucine-zipperAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi332 – 341Asp/Glu-rich (acidic)10

Sequence similaritiesi

Belongs to the AAR2 family.Curated

Phylogenomic databases

HOGENOMiHOG000033742.
InParanoidiP32357.
KOiK13205.
OMAiGSSLQWH.
OrthoDBiEOG092C4BSY.

Family and domain databases

CDDicd13778. Aar2_C. 1 hit.
cd13777. Aar2_N. 1 hit.
InterProiIPR007946. AAR2.
IPR033648. AAR2_C.
IPR033647. Aar2_N.
[Graphical view]
PANTHERiPTHR12689. PTHR12689. 1 hit.
PfamiPF05282. AAR2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32357-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTVPFTSAP IEVTIGIDQY SFNVKENQPF HGIKDIPIGH VHVIHFQHAD
60 70 80 90 100
NSSMRYGYWF DCRMGNFYIQ YDPKDGLYKM MEERDGAKFE NIVHNFKERQ
110 120 130 140 150
MMVSYPKIDE DDTWYNLTEF VQMDKIRKIV RKDENQFSYV DSSMTTVQEN
160 170 180 190 200
ELLKSSLQKA GSKMEAKNED DPAHSLNYTV INFKSREAIR PGHEMEDFLD
210 220 230 240 250
KSYYLNTVML QGIFKNSSNY FGELQFAFLN AMFFGNYGSS LQWHAMIELI
260 270 280 290 300
CSSATVPKHM LDKLDEILYY QIKTLPEQYS DILLNERVWN ICLYSSFQKN
310 320 330 340 350
SLHNTEKIME NKYPELLGKD NEDDALIYGI SDEERDDEDD EHNPTIVGGL

YYQRP
Length:355
Mass (Da):41,688
Last modified:October 1, 1993 - v1
Checksum:i1F030D821D974131
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90455 Genomic DNA. Translation: BAA14421.1.
Z26879 Genomic DNA. Translation: CAA81524.1.
Z35835 Genomic DNA. Translation: CAA84894.1.
M36115 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07048.1.
PIRiS18510.
RefSeqiNP_009479.1. NM_001178314.1.

Genome annotation databases

EnsemblFungiiYBL074C; YBL074C; YBL074C.
GeneIDi852205.
KEGGisce:YBL074C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90455 Genomic DNA. Translation: BAA14421.1.
Z26879 Genomic DNA. Translation: CAA81524.1.
Z35835 Genomic DNA. Translation: CAA84894.1.
M36115 Genomic DNA. No translation available.
BK006936 Genomic DNA. Translation: DAA07048.1.
PIRiS18510.
RefSeqiNP_009479.1. NM_001178314.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SBSX-ray2.10A1-355[»]
3SBTX-ray1.80B1-355[»]
3ZEFX-ray3.10A/D1-355[»]
4I43X-ray2.00A1-355[»]
4ILGX-ray2.10A1-355[»]
4ILHX-ray1.85B1-331[»]
4ILIX-ray3.20A/B1-318[»]
ProteinModelPortaliP32357.
SMRiP32357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32628. 17 interactors.
DIPiDIP-6642N.
IntActiP32357. 8 interactors.
MINTiMINT-663730.

PTM databases

iPTMnetiP32357.

Proteomic databases

MaxQBiP32357.
PRIDEiP32357.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL074C; YBL074C; YBL074C.
GeneIDi852205.
KEGGisce:YBL074C.

Organism-specific databases

EuPathDBiFungiDB:YBL074C.
SGDiS000000170. AAR2.

Phylogenomic databases

HOGENOMiHOG000033742.
InParanoidiP32357.
KOiK13205.
OMAiGSSLQWH.
OrthoDBiEOG092C4BSY.

Enzyme and pathway databases

BioCyciYEAST:G3O-28966-MONOMER.

Miscellaneous databases

PROiP32357.

Family and domain databases

CDDicd13778. Aar2_C. 1 hit.
cd13777. Aar2_N. 1 hit.
InterProiIPR007946. AAR2.
IPR033648. AAR2_C.
IPR033647. Aar2_N.
[Graphical view]
PANTHERiPTHR12689. PTHR12689. 1 hit.
PfamiPF05282. AAR2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAR2_YEAST
AccessioniPrimary (citable) accession number: P32357
Secondary accession number(s): D6VPS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7330 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.