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Protein

Neutral trehalase

Gene

NTH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 1840 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.

Enzyme regulationi

Activated by cAMP-dependent phosphorylation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei302SubstrateBy similarity1
Binding sitei346SubstrateBy similarity1
Binding sitei355SubstrateBy similarity1
Binding sitei476Substrate; via carbonyl oxygenBy similarity1
Active sitei478Proton donor/acceptorBy similarity1
Active sitei674Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • alpha,alpha-trehalase activity Source: SGD
  • calcium ion binding Source: InterPro

GO - Biological processi

  • cellular response to desiccation Source: SGD
  • trehalose catabolic process Source: SGD

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:YDR001C-MONOMER
YEAST:YDR001C-MONOMER

Protein family/group databases

CAZyiGH37 Glycoside Hydrolase Family 37

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral trehalase (EC:3.2.1.28)
Alternative name(s):
Alpha,alpha-trehalase
Alpha,alpha-trehalose glucohydrolase
Gene namesi
Name:NTH1
Synonyms:NTH
Ordered Locus Names:YDR001C
ORF Names:YD8119.07C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR001C
SGDiS000002408 NTH1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001737982 – 751Neutral trehalaseAdd BLAST750

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei20PhosphoserineCombined sources1
Modified residuei21PhosphoserineCombined sources1
Modified residuei23PhosphoserineCombined sources1
Modified residuei58PhosphothreonineCombined sources1
Modified residuei60PhosphoserineCombined sources1
Modified residuei66PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32356
PaxDbiP32356
PRIDEiP32356

PTM databases

iPTMnetiP32356

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32054, 91 interactors
DIPiDIP-1479N
IntActiP32356, 16 interactors
MINTiP32356
STRINGi4932.YDR001C

Structurei

Secondary structure

1751
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 35Combined sources4
Turni55 – 57Combined sources3
Turni67 – 73Combined sources7
Helixi87 – 92Combined sources6
Beta strandi96 – 100Combined sources5
Helixi102 – 113Combined sources12
Beta strandi129 – 135Combined sources7
Turni136 – 140Combined sources5
Beta strandi141 – 148Combined sources8
Helixi149 – 164Combined sources16
Beta strandi168 – 173Combined sources6
Helixi174 – 176Combined sources3
Helixi181 – 191Combined sources11
Helixi193 – 197Combined sources5
Turni203 – 205Combined sources3
Helixi206 – 210Combined sources5
Helixi218 – 220Combined sources3
Beta strandi224 – 227Combined sources4
Helixi232 – 244Combined sources13
Helixi246 – 248Combined sources3
Beta strandi251 – 254Combined sources4
Helixi261 – 266Combined sources6
Turni267 – 269Combined sources3
Beta strandi278 – 282Combined sources5
Turni284 – 286Combined sources3
Beta strandi289 – 293Combined sources5
Helixi309 – 320Combined sources12
Helixi324 – 341Combined sources18
Beta strandi346 – 349Combined sources4
Helixi350 – 352Combined sources3
Helixi361 – 371Combined sources11
Helixi374 – 376Combined sources3
Helixi378 – 397Combined sources20
Turni398 – 400Combined sources3
Turni402 – 404Combined sources3
Turni407 – 409Combined sources3
Turni427 – 430Combined sources4
Helixi431 – 433Combined sources3
Helixi449 – 453Combined sources5
Helixi460 – 474Combined sources15
Turni481 – 486Combined sources6
Helixi487 – 489Combined sources3
Helixi493 – 513Combined sources21
Turni514 – 516Combined sources3
Turni521 – 523Combined sources3
Helixi529 – 547Combined sources19
Turni550 – 553Combined sources4
Turni560 – 563Combined sources4
Helixi571 – 574Combined sources4
Helixi575 – 578Combined sources4
Helixi584 – 593Combined sources10
Helixi595 – 598Combined sources4
Helixi609 – 612Combined sources4
Beta strandi617 – 619Combined sources3
Beta strandi623 – 625Combined sources3
Helixi631 – 643Combined sources13
Helixi647 – 667Combined sources21
Turni668 – 670Combined sources3
Beta strandi674 – 677Combined sources4
Beta strandi699 – 701Combined sources3
Helixi707 – 717Combined sources11
Helixi722 – 729Combined sources8
Helixi734 – 740Combined sources7
Helixi745 – 749Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JTAX-ray2.72A1-751[»]
5M4AX-ray2.90A153-751[»]
5N6NX-ray2.29C1-751[»]
5NISX-ray3.15A100-751[»]
ProteinModelPortaliP32356
SMRiP32356
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 310Substrate bindingBy similarity2
Regioni355 – 357Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyl hydrolase 37 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006949
HOGENOMiHOG000192885
InParanoidiP32356
KOiK01194
OMAiMMTTAFV
OrthoDBiEOG092C0UDQ

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR001661 Glyco_hydro_37
IPR018232 Glyco_hydro_37_CS
IPR011120 Trehalase_Ca-bd
PANTHERiPTHR23403 PTHR23403, 1 hit
PfamiView protein in Pfam
PF01204 Trehalase, 1 hit
PF07492 Trehalase_Ca-bi, 1 hit
PRINTSiPR00744 GLHYDRLASE37
SUPFAMiSSF48208 SSF48208, 1 hit
PROSITEiView protein in PROSITE
PS00927 TREHALASE_1, 1 hit
PS00928 TREHALASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32356-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQVNTSQGP VAQGRQRRLS SLSEFNDPFS NAEVYYGPPT DPRKQKQAKP
60 70 80 90 100
AKINRTRTMS VFDNVSPFKK TGFGKLQQTR RGSEDDTYSS SQGNRRFFIE
110 120 130 140 150
DVDKTLNELL AAEDTDKNYQ ITIEDTGPKV LKVGTANSYG YKHINIRGTY
160 170 180 190 200
MLSNLLQELT IAKSFGRHQI FLDEARINEN PVNRLSRLIN TQFWNSLTRR
210 220 230 240 250
VDLNNVGEIA KDTKIDTPGA KNPRIYVPYD CPEQYEFYVQ ASQMHPSLKL
260 270 280 290 300
EVEYLPKKIT AEYVKSVNDT PGLLALAMEE HFNPSTGEKT LIGYPYAVPG
310 320 330 340 350
GRFNELYGWD SYMMALGLLE ANKTDVARGM VEHFIFEINH YGKILNANRS
360 370 380 390 400
YYLCRSQPPF LTEMALVVFK KLGGRSNPDA VDLLKRAFQA SIKEYKTVWT
410 420 430 440 450
ASPRLDPETG LSRYHPNGLG IPPETESDHF DTVLLPYASK HGVTLDEFKQ
460 470 480 490 500
LYNDGKIKEP KLDEFFLHDR GVRESGHDTT YRFEGVCAYL ATIDLNSLLY
510 520 530 540 550
KYEIDIADFI KEFCDDKYED PLDHSITTSA MWKEMAKIRQ EKITKYMWDD
560 570 580 590 600
ESGFFFDYNT KIKHRTSYES ATTFWALWAG LATKEQAQKM VEKALPKLEM
610 620 630 640 650
LGGLAACTER SRGPISISRP IRQWDYPFGW APHQILAWEG LRSYGYLTVT
660 670 680 690 700
NRLAYRWLFM MTKAFVDYNG IVVEKYDVTR GTDPHRVEAE YGNQGADFKG
710 720 730 740 750
AATEGFGWVN ASYILGLKYM NSHARRALGA CIPPISFFSS LRPQERNLYG

L
Length:751
Mass (Da):85,879
Last modified:October 1, 1996 - v3
Checksum:iE6687F2E9377E49F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti514 – 515CD → NN in AAA66896 (PubMed:3537685).Curated2
Sequence conflicti712S → R in CAA46718 (PubMed:8444853).Curated1
Sequence conflicti712S → R in AAA66896 (PubMed:3537685).Curated1
Sequence conflicti723 – 724HA → YE in CAA46718 (PubMed:8444853).Curated2
Sequence conflicti723 – 724HA → YE in AAA66896 (PubMed:3537685).Curated2
Sequence conflicti727 – 728AL → EI in CAA46718 (PubMed:8444853).Curated2
Sequence conflicti727 – 728AL → EI in AAA66896 (PubMed:3537685).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65925 Genomic DNA Translation: CAA46718.1
Z48008 Genomic DNA Translation: CAA88061.1
M13000 Genomic DNA Translation: AAA66896.1
BK006938 Genomic DNA Translation: DAA11848.1
PIRiS50982
RefSeqiNP_010284.1, NM_001180309.1

Genome annotation databases

EnsemblFungiiYDR001C; YDR001C; YDR001C
GeneIDi851564
KEGGisce:YDR001C

Similar proteinsi

Entry informationi

Entry nameiTREA_YEAST
AccessioniPrimary (citable) accession number: P32356
Secondary accession number(s): D6VRY8, E9P9U7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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