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Protein

Neutral trehalase

Gene

NTH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.

Enzyme regulationi

Activated by cAMP-dependent phosphorylation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei302SubstrateBy similarity1
Binding sitei346SubstrateBy similarity1
Binding sitei355SubstrateBy similarity1
Binding sitei476Substrate; via carbonyl oxygenBy similarity1
Active sitei478Proton donor/acceptorBy similarity1
Active sitei674Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • alpha,alpha-trehalase activity Source: SGD
  • calcium ion binding Source: InterPro

GO - Biological processi

  • cellular response to desiccation Source: SGD
  • trehalose catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:YDR001C-MONOMER.
YEAST:YDR001C-MONOMER.

Protein family/group databases

CAZyiGH37. Glycoside Hydrolase Family 37.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral trehalase (EC:3.2.1.28)
Alternative name(s):
Alpha,alpha-trehalase
Alpha,alpha-trehalose glucohydrolase
Gene namesi
Name:NTH1
Synonyms:NTH
Ordered Locus Names:YDR001C
ORF Names:YD8119.07C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR001C.
SGDiS000002408. NTH1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001737982 – 751Neutral trehalaseAdd BLAST750

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei20PhosphoserineCombined sources1
Modified residuei21PhosphoserineCombined sources1
Modified residuei23PhosphoserineCombined sources1
Modified residuei58PhosphothreonineCombined sources1
Modified residuei60PhosphoserineCombined sources1
Modified residuei66PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32356.
PRIDEiP32356.

PTM databases

iPTMnetiP32356.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
BMH1P293116EBI-19509,EBI-3661
BMH2P347308EBI-19509,EBI-3672

Protein-protein interaction databases

BioGridi32054. 60 interactors.
DIPiDIP-1479N.
IntActiP32356. 16 interactors.
MINTiMINT-392566.

Structurei

3D structure databases

ProteinModelPortaliP32356.
SMRiP32356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 310Substrate bindingBy similarity2
Regioni355 – 357Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyl hydrolase 37 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006949.
HOGENOMiHOG000192885.
InParanoidiP32356.
KOiK01194.
OMAiMNSHARR.
OrthoDBiEOG092C0UDQ.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR001661. Glyco_hydro_37.
IPR018232. Glyco_hydro_37_CS.
IPR011120. Trehalase_Ca-bd.
[Graphical view]
PANTHERiPTHR23403. PTHR23403. 3 hits.
PfamiPF01204. Trehalase. 1 hit.
PF07492. Trehalase_Ca-bi. 1 hit.
[Graphical view]
PRINTSiPR00744. GLHYDRLASE37.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00927. TREHALASE_1. 1 hit.
PS00928. TREHALASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32356-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQVNTSQGP VAQGRQRRLS SLSEFNDPFS NAEVYYGPPT DPRKQKQAKP
60 70 80 90 100
AKINRTRTMS VFDNVSPFKK TGFGKLQQTR RGSEDDTYSS SQGNRRFFIE
110 120 130 140 150
DVDKTLNELL AAEDTDKNYQ ITIEDTGPKV LKVGTANSYG YKHINIRGTY
160 170 180 190 200
MLSNLLQELT IAKSFGRHQI FLDEARINEN PVNRLSRLIN TQFWNSLTRR
210 220 230 240 250
VDLNNVGEIA KDTKIDTPGA KNPRIYVPYD CPEQYEFYVQ ASQMHPSLKL
260 270 280 290 300
EVEYLPKKIT AEYVKSVNDT PGLLALAMEE HFNPSTGEKT LIGYPYAVPG
310 320 330 340 350
GRFNELYGWD SYMMALGLLE ANKTDVARGM VEHFIFEINH YGKILNANRS
360 370 380 390 400
YYLCRSQPPF LTEMALVVFK KLGGRSNPDA VDLLKRAFQA SIKEYKTVWT
410 420 430 440 450
ASPRLDPETG LSRYHPNGLG IPPETESDHF DTVLLPYASK HGVTLDEFKQ
460 470 480 490 500
LYNDGKIKEP KLDEFFLHDR GVRESGHDTT YRFEGVCAYL ATIDLNSLLY
510 520 530 540 550
KYEIDIADFI KEFCDDKYED PLDHSITTSA MWKEMAKIRQ EKITKYMWDD
560 570 580 590 600
ESGFFFDYNT KIKHRTSYES ATTFWALWAG LATKEQAQKM VEKALPKLEM
610 620 630 640 650
LGGLAACTER SRGPISISRP IRQWDYPFGW APHQILAWEG LRSYGYLTVT
660 670 680 690 700
NRLAYRWLFM MTKAFVDYNG IVVEKYDVTR GTDPHRVEAE YGNQGADFKG
710 720 730 740 750
AATEGFGWVN ASYILGLKYM NSHARRALGA CIPPISFFSS LRPQERNLYG

L
Length:751
Mass (Da):85,879
Last modified:October 1, 1996 - v3
Checksum:iE6687F2E9377E49F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti514 – 515CD → NN in AAA66896 (PubMed:3537685).Curated2
Sequence conflicti712S → R in CAA46718 (PubMed:8444853).Curated1
Sequence conflicti712S → R in AAA66896 (PubMed:3537685).Curated1
Sequence conflicti723 – 724HA → YE in CAA46718 (PubMed:8444853).Curated2
Sequence conflicti723 – 724HA → YE in AAA66896 (PubMed:3537685).Curated2
Sequence conflicti727 – 728AL → EI in CAA46718 (PubMed:8444853).Curated2
Sequence conflicti727 – 728AL → EI in AAA66896 (PubMed:3537685).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65925 Genomic DNA. Translation: CAA46718.1.
Z48008 Genomic DNA. Translation: CAA88061.1.
M13000 Genomic DNA. Translation: AAA66896.1.
BK006938 Genomic DNA. Translation: DAA11848.1.
PIRiS50982.
RefSeqiNP_010284.1. NM_001180309.1.

Genome annotation databases

EnsemblFungiiYDR001C; YDR001C; YDR001C.
GeneIDi851564.
KEGGisce:YDR001C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65925 Genomic DNA. Translation: CAA46718.1.
Z48008 Genomic DNA. Translation: CAA88061.1.
M13000 Genomic DNA. Translation: AAA66896.1.
BK006938 Genomic DNA. Translation: DAA11848.1.
PIRiS50982.
RefSeqiNP_010284.1. NM_001180309.1.

3D structure databases

ProteinModelPortaliP32356.
SMRiP32356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32054. 60 interactors.
DIPiDIP-1479N.
IntActiP32356. 16 interactors.
MINTiMINT-392566.

Protein family/group databases

CAZyiGH37. Glycoside Hydrolase Family 37.

PTM databases

iPTMnetiP32356.

Proteomic databases

MaxQBiP32356.
PRIDEiP32356.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR001C; YDR001C; YDR001C.
GeneIDi851564.
KEGGisce:YDR001C.

Organism-specific databases

EuPathDBiFungiDB:YDR001C.
SGDiS000002408. NTH1.

Phylogenomic databases

GeneTreeiENSGT00390000006949.
HOGENOMiHOG000192885.
InParanoidiP32356.
KOiK01194.
OMAiMNSHARR.
OrthoDBiEOG092C0UDQ.

Enzyme and pathway databases

BioCyciMetaCyc:YDR001C-MONOMER.
YEAST:YDR001C-MONOMER.

Miscellaneous databases

PROiP32356.

Family and domain databases

InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR001661. Glyco_hydro_37.
IPR018232. Glyco_hydro_37_CS.
IPR011120. Trehalase_Ca-bd.
[Graphical view]
PANTHERiPTHR23403. PTHR23403. 3 hits.
PfamiPF01204. Trehalase. 1 hit.
PF07492. Trehalase_Ca-bi. 1 hit.
[Graphical view]
PRINTSiPR00744. GLHYDRLASE37.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00927. TREHALASE_1. 1 hit.
PS00928. TREHALASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTREA_YEAST
AccessioniPrimary (citable) accession number: P32356
Secondary accession number(s): D6VRY8, E9P9U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 148 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1840 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.