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Protein

Delta(7)-sterol 5(6)-desaturase

Gene

ERG3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the introduction of a C-5 double bond in the B ring of ergosterol. May contribute to the regulation of ergosterol biosynthesis.1 Publication

Catalytic activityi

A Delta(7)-sterol + 2 ferrocytochrome b5 + O2 + 2 H+ = a Delta(5,7)-sterol + 2 ferricytochrome b5 + 2 H2O.1 Publication

Cofactori

Fe cationBy similarity

Pathwayi: ergosterol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes ergosterol from zymosterol.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Sterol 24-C-methyltransferase (ERG6)
  2. C-8 sterol isomerase (ERG2)
  3. Delta(7)-sterol 5(6)-desaturase (ERG3)
  4. Cytochrome P450 61 (ERG5)
  5. Delta(24(24(1)))-sterol reductase (ERG4)
This subpathway is part of the pathway ergosterol biosynthesis, which is itself part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ergosterol from zymosterol, the pathway ergosterol biosynthesis and in Steroid metabolism.

GO - Molecular functioni

  • C-5 sterol desaturase activity Source: SGD
  • iron ion binding Source: InterPro

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
  • fatty acid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciMetaCyc:YLR056W-MONOMER.
YEAST:YLR056W-MONOMER.
ReactomeiR-SCE-2426168. Activation of gene expression by SREBF (SREBP).
R-SCE-6807047. Cholesterol biosynthesis via desmosterol.
R-SCE-6807062. Cholesterol biosynthesis via lathosterol.
UniPathwayiUPA00768; UER00762.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta(7)-sterol 5(6)-desaturase (EC:1.14.19.201 Publication)
Alternative name(s):
C-5 sterol desaturase
Ergosterol Delta(5,6) desaturase
Sterol-C5-desaturase
Gene namesi
Name:ERG31 Publication
Synonyms:PSO6, SYR1
Ordered Locus Names:YLR056W
ORF Names:L2150
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR056W.
SGDiS000004046. ERG3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 92CytoplasmicSequence analysisAdd BLAST92
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 140LumenalSequence analysisAdd BLAST27
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 242CytoplasmicSequence analysisAdd BLAST81
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 365LumenalSequence analysisAdd BLAST102

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001170271 – 365Delta(7)-sterol 5(6)-desaturaseAdd BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki324Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki344Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP32353.
PRIDEiP32353.

Interactioni

Subunit structurei

Interacts with ERG28.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ERG25P530453EBI-6554,EBI-6506

Protein-protein interaction databases

BioGridi31331. 349 interactors.
DIPiDIP-4136N.
IntActiP32353. 11 interactors.
MINTiMINT-481056.

Structurei

3D structure databases

ProteinModelPortaliP32353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi200 – 204Histidine box-15
Motifi213 – 217Histidine box-25
Motifi288 – 292Histidine box-35

Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075101.
HOGENOMiHOG000200579.
InParanoidiP32353.
KOiK00227.
OMAiHHKLIYK.
OrthoDBiEOG092C3V4R.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32353-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLVLEVADH YVLDDLYAKV LPASLAANIP VKWQKLLGLN SGFSNSTILQ
60 70 80 90 100
ETLNSKNAVK ECRRFYGQVP FLFDMSTTSF ASLLPRSSIL REFLSLWVIV
110 120 130 140 150
TIFGLLLYLF TASLSYVFVF DKSIFNHPRY LKNQMAMEIK LAVSAIPWMS
160 170 180 190 200
MLTVPWFVME LNGHSKLYMK IDYENHGVRK LIIEYFTFIF FTDCGVYLAH
210 220 230 240 250
RWLHWPRVYR ALHKPHHKWL VCTPFASHSF HPVDGFLQSI SYHIYPLILP
260 270 280 290 300
LHKVSYLILF TFVNFWTVMI HDGQYLSNNP AVNGTACHTV HHLYFNYNYG
310 320 330 340 350
QFTTLWDRLG GSYRRPDDSL FDPKLRDAKE TWDAQVKEVE HFIKEVEGDD
360
NDRIYENDPN TKKNN
Length:365
Mass (Da):42,730
Last modified:October 1, 1993 - v1
Checksum:i7F441DA6927A711C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62623 Genomic DNA. Translation: AAA34594.1.
S46162 Genomic DNA. Translation: AAB39844.1.
M64989 Genomic DNA. Translation: AAA34595.1.
D14299 Genomic DNA. Translation: BAA20292.1.
X94607 Genomic DNA. Translation: CAA64303.1.
Z73228 Genomic DNA. Translation: CAA97586.1.
AY692996 Genomic DNA. Translation: AAT93015.1.
BK006945 Genomic DNA. Translation: DAA09374.1.
PIRiJQ1146.
RefSeqiNP_013157.1. NM_001181943.1.

Genome annotation databases

EnsemblFungiiYLR056W; YLR056W; YLR056W.
GeneIDi850745.
KEGGisce:YLR056W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62623 Genomic DNA. Translation: AAA34594.1.
S46162 Genomic DNA. Translation: AAB39844.1.
M64989 Genomic DNA. Translation: AAA34595.1.
D14299 Genomic DNA. Translation: BAA20292.1.
X94607 Genomic DNA. Translation: CAA64303.1.
Z73228 Genomic DNA. Translation: CAA97586.1.
AY692996 Genomic DNA. Translation: AAT93015.1.
BK006945 Genomic DNA. Translation: DAA09374.1.
PIRiJQ1146.
RefSeqiNP_013157.1. NM_001181943.1.

3D structure databases

ProteinModelPortaliP32353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31331. 349 interactors.
DIPiDIP-4136N.
IntActiP32353. 11 interactors.
MINTiMINT-481056.

Proteomic databases

MaxQBiP32353.
PRIDEiP32353.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR056W; YLR056W; YLR056W.
GeneIDi850745.
KEGGisce:YLR056W.

Organism-specific databases

EuPathDBiFungiDB:YLR056W.
SGDiS000004046. ERG3.

Phylogenomic databases

GeneTreeiENSGT00550000075101.
HOGENOMiHOG000200579.
InParanoidiP32353.
KOiK00227.
OMAiHHKLIYK.
OrthoDBiEOG092C3V4R.

Enzyme and pathway databases

UniPathwayiUPA00768; UER00762.
BioCyciMetaCyc:YLR056W-MONOMER.
YEAST:YLR056W-MONOMER.
ReactomeiR-SCE-2426168. Activation of gene expression by SREBF (SREBP).
R-SCE-6807047. Cholesterol biosynthesis via desmosterol.
R-SCE-6807062. Cholesterol biosynthesis via lathosterol.

Miscellaneous databases

PROiP32353.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERG3_YEAST
AccessioniPrimary (citable) accession number: P32353
Secondary accession number(s): D6VY58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 36200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.