P32352 (ERG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-8 sterol isomerase
EC=5.-.-.-

Alternative name(s):
Delta-8--delta-7 sterol isomerase

Gene names

Name:	ERG2
Ordered Locus Names:	YMR202W
ORF Names:	YM8325.03

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 2/5.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Potential Ref.6.
Miscellaneous	This protein is a major target of morpholine antibiotics. Present with 2640 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ERG2 family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ergosterol biosynthetic process Inferred from mutant phenotype PubMed 18459942. Source: SGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	C-8 sterol isomerase activity Inferred from mutant phenotype PubMed 18459942. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	C-8 sterol isomerase		PRO_0000087021
Regions
	Transmembrane	3 – 23	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P32352 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 9E17143542DDBB75	FASTA	222	24,896
	10 20 30 40 50 60 MKFFPLLLLI GVVGYIMNVL FTTWLPTNYM FDPKTLNEIC NSVISKHNAA EGLSTEDLLQ 70 80 90 100 110 120 DVRDALASHY GDEYINRYVK EEWVFNNAGG AMGQMIILHA SVSEYLILFG TAVGTEGHTG 130 140 150 160 170 180 VHFADDYFTI LHGTQIAALP YATEAEVYTP GMTHHLKKGY AKQYSMPGGS FALELAQGWI 190 200 210 220 PCMLPFGFLD TFSSTLDLYT LYRTVYLTAR DMGKNLLQNK KF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the gene encoding yeast C-8 sterol isomerase." Arthington B.A., Hoskins J.A., Skatrud P.L., Bard M. Gene 107:173-174(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy." Natter K., Leitner P., Faschinger A., Wolinski H., McCraith S., Fields S., Kohlwein S.D. Mol. Cell. Proteomics 4:662-672(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M74037 Genomic DNA. Translation: AAA34593.1. Z48755 Genomic DNA. Translation: CAA88643.1. AY558122 Genomic DNA. Translation: AAS56448.1. BK006946 Genomic DNA. Translation: DAA10101.1.
PIR	JH0488.
RefSeq	NP_013929.1. NM_001182709.1.
3D structure databases
ProteinModelPortal	P32352.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5068N.
IntAct	P32352. 7 interactions.
MINT	MINT-515226.
STRING	4932.YMR202W.
Proteomic databases
PaxDb	P32352.
PeptideAtlas	P32352.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR202W; YMR202W; YMR202W.
GeneID	855242.
KEGG	sce:YMR202W.
Organism-specific databases
CYGD	YMR202w.
SGD	S000004815. ERG2.
Phylogenomic databases
eggNOG	NOG296707.
GeneTree	ENSGT00390000012082.
HOGENOM	HOG000211245.
KO	K09829.
OMA	WNNAGGA.
OrthoDB	EOG45TGXC.
Enzyme and pathway databases
BioCyc	MetaCyc:YMR202W-MONOMER.
UniPathway	UPA00768; UER00761.
Gene expression databases
Genevestigator	P32352.
GermOnline	YMR202W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006716. ERG2_sigma1_rcpt-like. [Graphical view]
Pfam	PF04622. ERG2_Sigma1R. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P32352.
ChEMBL	CHEMBL3224.
NextBio	978798.

Entry information

Entry name

ERG2_YEAST

Accession

Primary (citable) accession number: P32352
Secondary accession number(s): D6W027

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program