Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

C-8 sterol isomerase

Gene

ERG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols.

Pathwayi: ergosterol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes ergosterol from zymosterol.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Sterol 24-C-methyltransferase (ERG6)
  2. C-8 sterol isomerase (ERG2)
  3. Delta(7)-sterol 5(6)-desaturase (ERG3)
  4. Cytochrome P450 61 (ERG5)
  5. Delta(24(24(1)))-sterol reductase (ERG4)
This subpathway is part of the pathway ergosterol biosynthesis, which is itself part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ergosterol from zymosterol, the pathway ergosterol biosynthesis and in Steroid metabolism.

GO - Molecular functioni

  • C-8 sterol isomerase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YMR202W-MONOMER.
YEAST:YMR202W-MONOMER.
UniPathwayiUPA00768; UER00761.

Names & Taxonomyi

Protein namesi
Recommended name:
C-8 sterol isomerase (EC:5.-.-.-)
Alternative name(s):
Delta-8--delta-7 sterol isomerase
Gene namesi
Name:ERG2
Ordered Locus Names:YMR202W
ORF Names:YM8325.03
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR202W.
SGDiS000004815. ERG2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3224.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000870211 – 222C-8 sterol isomeraseAdd BLAST222

Proteomic databases

MaxQBiP32352.
PRIDEiP32352.
TopDownProteomicsiP32352.

Interactioni

Protein-protein interaction databases

BioGridi35380. 302 interactors.
DIPiDIP-5068N.
IntActiP32352. 7 interactors.
MINTiMINT-515226.

Chemistry databases

BindingDBiP32352.

Structurei

3D structure databases

ProteinModelPortaliP32352.
SMRiP32352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ERG2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000012082.
HOGENOMiHOG000211245.
InParanoidiP32352.
KOiK09829.
OMAiDFPTLWK.
OrthoDBiEOG092C47C3.

Family and domain databases

InterProiIPR006716. ERG2_sigma1_rcpt-like.
[Graphical view]
PfamiPF04622. ERG2_Sigma1R. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32352-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFFPLLLLI GVVGYIMNVL FTTWLPTNYM FDPKTLNEIC NSVISKHNAA
60 70 80 90 100
EGLSTEDLLQ DVRDALASHY GDEYINRYVK EEWVFNNAGG AMGQMIILHA
110 120 130 140 150
SVSEYLILFG TAVGTEGHTG VHFADDYFTI LHGTQIAALP YATEAEVYTP
160 170 180 190 200
GMTHHLKKGY AKQYSMPGGS FALELAQGWI PCMLPFGFLD TFSSTLDLYT
210 220
LYRTVYLTAR DMGKNLLQNK KF
Length:222
Mass (Da):24,896
Last modified:October 1, 1993 - v1
Checksum:i9E17143542DDBB75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74037 Genomic DNA. Translation: AAA34593.1.
Z48755 Genomic DNA. Translation: CAA88643.1.
AY558122 Genomic DNA. Translation: AAS56448.1.
BK006946 Genomic DNA. Translation: DAA10101.1.
PIRiJH0488.
RefSeqiNP_013929.1. NM_001182709.1.

Genome annotation databases

EnsemblFungiiYMR202W; YMR202W; YMR202W.
GeneIDi855242.
KEGGisce:YMR202W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74037 Genomic DNA. Translation: AAA34593.1.
Z48755 Genomic DNA. Translation: CAA88643.1.
AY558122 Genomic DNA. Translation: AAS56448.1.
BK006946 Genomic DNA. Translation: DAA10101.1.
PIRiJH0488.
RefSeqiNP_013929.1. NM_001182709.1.

3D structure databases

ProteinModelPortaliP32352.
SMRiP32352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35380. 302 interactors.
DIPiDIP-5068N.
IntActiP32352. 7 interactors.
MINTiMINT-515226.

Chemistry databases

BindingDBiP32352.
ChEMBLiCHEMBL3224.

Proteomic databases

MaxQBiP32352.
PRIDEiP32352.
TopDownProteomicsiP32352.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR202W; YMR202W; YMR202W.
GeneIDi855242.
KEGGisce:YMR202W.

Organism-specific databases

EuPathDBiFungiDB:YMR202W.
SGDiS000004815. ERG2.

Phylogenomic databases

GeneTreeiENSGT00390000012082.
HOGENOMiHOG000211245.
InParanoidiP32352.
KOiK09829.
OMAiDFPTLWK.
OrthoDBiEOG092C47C3.

Enzyme and pathway databases

UniPathwayiUPA00768; UER00761.
BioCyciMetaCyc:YMR202W-MONOMER.
YEAST:YMR202W-MONOMER.

Miscellaneous databases

PROiP32352.

Family and domain databases

InterProiIPR006716. ERG2_sigma1_rcpt-like.
[Graphical view]
PfamiPF04622. ERG2_Sigma1R. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERG2_YEAST
AccessioniPrimary (citable) accession number: P32352
Secondary accession number(s): D6W027
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is a major target of morpholine antibiotics.
Present with 2640 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.