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Protein

C-8 sterol isomerase

Gene

ERG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols.

Miscellaneous

This protein is a major target of morpholine antibiotics.
Present with 2640 molecules/cell in log phase SD medium.1 Publication

Pathwayi: ergosterol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes ergosterol from zymosterol.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Sterol 24-C-methyltransferase (ERG6)
  2. C-8 sterol isomerase (ERG2)
  3. Delta(7)-sterol 5(6)-desaturase (ERG3)
  4. Cytochrome P450 61 (ERG5)
  5. Delta(24(24(1)))-sterol reductase (ERG4)
This subpathway is part of the pathway ergosterol biosynthesis, which is itself part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ergosterol from zymosterol, the pathway ergosterol biosynthesis and in Steroid metabolism.

GO - Molecular functioni

  • C-8 sterol isomerase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD

Keywordsi

Molecular functionIsomerase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YMR202W-MONOMER
YEAST:YMR202W-MONOMER
UniPathwayiUPA00768; UER00761

Names & Taxonomyi

Protein namesi
Recommended name:
C-8 sterol isomerase (EC:5.-.-.-)
Alternative name(s):
Delta-8--delta-7 sterol isomerase
Gene namesi
Name:ERG2
Ordered Locus Names:YMR202W
ORF Names:YM8325.03
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR202W
SGDiS000004815 ERG2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3224

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000870211 – 222C-8 sterol isomeraseAdd BLAST222

Proteomic databases

MaxQBiP32352
PaxDbiP32352
PRIDEiP32352
TopDownProteomicsiP32352

Interactioni

Protein-protein interaction databases

BioGridi35380400 interactors.
DIPiDIP-5068N
IntActiP32352 7 interactors.
STRINGi4932.YMR202W

Chemistry databases

BindingDBiP32352

Structurei

3D structure databases

ProteinModelPortaliP32352
SMRiP32352
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ERG2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000012082
HOGENOMiHOG000211245
InParanoidiP32352
KOiK09829
OMAiWKEGTTK
OrthoDBiEOG092C47C3

Family and domain databases

InterProiView protein in InterPro
IPR006716 ERG2_sigma1_rcpt-like
PANTHERiPTHR10868 PTHR10868, 1 hit
PfamiView protein in Pfam
PF04622 ERG2_Sigma1R, 1 hit

Sequencei

Sequence statusi: Complete.

P32352-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFFPLLLLI GVVGYIMNVL FTTWLPTNYM FDPKTLNEIC NSVISKHNAA
60 70 80 90 100
EGLSTEDLLQ DVRDALASHY GDEYINRYVK EEWVFNNAGG AMGQMIILHA
110 120 130 140 150
SVSEYLILFG TAVGTEGHTG VHFADDYFTI LHGTQIAALP YATEAEVYTP
160 170 180 190 200
GMTHHLKKGY AKQYSMPGGS FALELAQGWI PCMLPFGFLD TFSSTLDLYT
210 220
LYRTVYLTAR DMGKNLLQNK KF
Length:222
Mass (Da):24,896
Last modified:October 1, 1993 - v1
Checksum:i9E17143542DDBB75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74037 Genomic DNA Translation: AAA34593.1
Z48755 Genomic DNA Translation: CAA88643.1
AY558122 Genomic DNA Translation: AAS56448.1
BK006946 Genomic DNA Translation: DAA10101.1
PIRiJH0488
RefSeqiNP_013929.1, NM_001182709.1

Genome annotation databases

EnsemblFungiiYMR202W; YMR202W; YMR202W
GeneIDi855242
KEGGisce:YMR202W

Similar proteinsi

Entry informationi

Entry nameiERG2_YEAST
AccessioniPrimary (citable) accession number: P32352
Secondary accession number(s): D6W027
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 28, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome