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P32350 (KNS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity protein kinase KNS1

EC=2.7.12.1
Gene names
Name:KNS1
Ordered Locus Names:YLL019C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length737 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Nonessential protein kinase. Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Post-translational modification

Phosphorylated (auto-) on Ser/Thr/Tyr. Ref.6

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 737737Dual specificity protein kinase KNS1
PRO_0000086155

Regions

Domain313 – 720408Protein kinase
Nucleotide binding319 – 3279ATP By similarity
Compositional bias143 – 1464Poly-Ser
Compositional bias148 – 1525Poly-Asn
Compositional bias673 – 6797Ser/Thr-rich

Sites

Active site4401Proton acceptor By similarity
Binding site3431ATP By similarity

Amino acid modifications

Modified residue5621Phosphothreonine Ref.7

Experimental info

Sequence conflict411 – 42111RFPGSHIQAIA → GSPALISGHC in AAA34724. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32350 [UniParc].

Last modified August 2, 2002. Version 2.
Checksum: F0224445BDEE8431

FASTA73783,843
        10         20         30         40         50         60 
MSQNIQIGTR KRSRANMNNS TTTGPANNTS SNKTFLDNFE ETRTNKLLDE MFARQNSFLT 

        70         80         90        100        110        120 
DNLRNSLDLN QADNPLRPRQ HQHQLFLDNE NAIELDEEPR IINTTINNSN NHNSSRVDED 

       130        140        150        160        170        180 
ADDDIIFIKE QPIQFSSPLI LPSSSSINNN NNIVTSNNPG CGTAATSNST YITTPKKFKK 

       190        200        210        220        230        240 
QRTISLPQLP LSKLSYQSNY FNVPDQTNAI VPRVTQTENE LLHLTGSCAK TLEGNKAVNL 

       250        260        270        280        290        300 
TIAHSTSPFS NPPAQIASLP QSNLKKQIGS SLRKFTSNGS SESASSNKSN FKTDKDGHYV 

       310        320        330        340        350        360 
YQENDIFGSG GRFVVKDLLG QGTFGKVLKC IDNKYEPNYV AVKVIRAVDR YREAAKTELR 

       370        380        390        400        410        420 
ILQTILNNDP QGQFQCLLLR ECFDYKNHIC LVTDLYGRSI YDFMCSNGIA RFPGSHIQAI 

       430        440        450        460        470        480 
ARQLIRSVCF LHDLGIIHTD LKPENILICD ETHIAQKLPL KTVQSLSKRR REASKGKRKI 

       490        500        510        520        530        540 
LKNPEIKIID FGSAIFHYEY HPPVISTRHY RAPEIVLGLG WSFPCDIWSI ACVLVELVIG 

       550        560        570        580        590        600 
ESLYPIHENL EHMAMMQRIN GTPFPTDIID KMFYKSKHKL GNSPSDLNST VIKHFDRKTL 

       610        620        630        640        650        660 
SLQWPEKNKR GDTITTEKSM KRVLQSCDRL DIYISKVLKQ DYGDSLSINW NLPPEKNWSL 

       670        680        690        700        710        720 
INSKLAWKRQ THSSSSSTTD ELDKETFLFW YWFIDLLRKM FEFDPTKRIT AKDALDHEWF 

       730 
NLGILDDGIA TYNNTQG 

« Hide

References

« Hide 'large scale' references
[1]"The KNS1 gene of Saccharomyces cerevisiae encodes a nonessential protein kinase homologue that is distantly related to members of the CDC28/cdc2 gene family."
Padmanabha R., Gehrung S., Snyder M.
Mol. Gen. Genet. 229:1-9(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The sequence of 32kb on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABC transporter homologous to the human multidrug resistance protein."
Purnelle B., Goffeau A.
Yeast 13:183-188(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Activity and autophosphorylation of LAMMER protein kinases."
Lee K., Du C., Horn M., Rabinow L.
J. Biol. Chem. 271:27299-27303(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Negative regulation of filamentous growth and flocculation by Lkh1, a fission yeast LAMMER kinase homolog."
Kim K.-H., Cho Y.-M., Kang W.-H., Kim J.-H., Byun K.-H., Park Y.-D., Bae K.-S., Park H.-M.
Biochem. Biophys. Res. Commun. 289:1237-1242(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: AUTOPHOSPHORYLATION.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M85200 Genomic DNA. Translation: AAA34724.1.
Z73125 Genomic DNA. Translation: CAA97468.1.
X97560 Genomic DNA. Translation: CAA66171.1.
Z73123 Genomic DNA. Translation: CAA97465.1.
BK006945 Genomic DNA. Translation: DAA09300.1.
PIRS64767.
RefSeqNP_013081.1. NM_001181839.1.

3D structure databases

ProteinModelPortalP32350.
SMRP32350. Positions 291-723.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31233. 68 interactions.
DIPDIP-1900N.
IntActP32350. 22 interactions.
MINTMINT-396154.
STRING4932.YLL019C.

Proteomic databases

PaxDbP32350.
PeptideAtlasP32350.
PRIDEP32350.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLL019C; YLL019C; YLL019C.
GeneID850641.
KEGGsce:YLL019C.

Organism-specific databases

CYGDYLL019c.
SGDS000003942. KNS1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00580000081366.
HOGENOMHOG000113240.
KOK08287.
OMALYPIHEN.
OrthoDBEOG7PGF0M.

Enzyme and pathway databases

BioCycYEAST:G3O-32124-MONOMER.
BRENDA2.7.12.1. 984.

Gene expression databases

GenevestigatorP32350.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 3 hits.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966573.

Entry information

Entry nameKNS1_YEAST
AccessionPrimary (citable) accession number: P32350
Secondary accession number(s): D6VXY4, Q12399
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: August 2, 2002
Last modified: April 16, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families