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Protein

Dual specificity protein kinase KNS1

Gene

KNS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nonessential protein kinase.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei343ATPPROSITE-ProRule annotation1
Active sitei440Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi319 – 327ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
  • protein serine/threonine kinase activity Source: SGD
  • protein tyrosine kinase activity Source: SGD

GO - Biological processi

  • negative regulation of transcription from RNA polymerase III promoter Source: SGD
  • peptidyl-serine phosphorylation Source: SGD
  • protein autophosphorylation Source: SGD
  • protein phosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32124-MONOMER.
BRENDAi2.7.12.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase KNS1 (EC:2.7.12.1)
Gene namesi
Name:KNS1
Ordered Locus Names:YLL019C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL019C.
SGDiS000003942. KNS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861551 – 737Dual specificity protein kinase KNS1Add BLAST737

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei562PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated (auto-) on Ser/Thr/Tyr.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP32350.
TopDownProteomicsiP32350.

PTM databases

iPTMnetiP32350.

Interactioni

Protein-protein interaction databases

BioGridi31233. 137 interactors.
DIPiDIP-1900N.
IntActiP32350. 22 interactors.
MINTiMINT-396154.

Structurei

3D structure databases

ProteinModelPortaliP32350.
SMRiP32350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini313 – 720Protein kinasePROSITE-ProRule annotationAdd BLAST408

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi143 – 146Poly-Ser4
Compositional biasi148 – 152Poly-Asn5
Compositional biasi673 – 679Ser/Thr-rich7

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00580000081366.
HOGENOMiHOG000113240.
InParanoidiP32350.
KOiK08287.
OMAiEHMAMMQ.
OrthoDBiEOG092C2DKP.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 3 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQNIQIGTR KRSRANMNNS TTTGPANNTS SNKTFLDNFE ETRTNKLLDE
60 70 80 90 100
MFARQNSFLT DNLRNSLDLN QADNPLRPRQ HQHQLFLDNE NAIELDEEPR
110 120 130 140 150
IINTTINNSN NHNSSRVDED ADDDIIFIKE QPIQFSSPLI LPSSSSINNN
160 170 180 190 200
NNIVTSNNPG CGTAATSNST YITTPKKFKK QRTISLPQLP LSKLSYQSNY
210 220 230 240 250
FNVPDQTNAI VPRVTQTENE LLHLTGSCAK TLEGNKAVNL TIAHSTSPFS
260 270 280 290 300
NPPAQIASLP QSNLKKQIGS SLRKFTSNGS SESASSNKSN FKTDKDGHYV
310 320 330 340 350
YQENDIFGSG GRFVVKDLLG QGTFGKVLKC IDNKYEPNYV AVKVIRAVDR
360 370 380 390 400
YREAAKTELR ILQTILNNDP QGQFQCLLLR ECFDYKNHIC LVTDLYGRSI
410 420 430 440 450
YDFMCSNGIA RFPGSHIQAI ARQLIRSVCF LHDLGIIHTD LKPENILICD
460 470 480 490 500
ETHIAQKLPL KTVQSLSKRR REASKGKRKI LKNPEIKIID FGSAIFHYEY
510 520 530 540 550
HPPVISTRHY RAPEIVLGLG WSFPCDIWSI ACVLVELVIG ESLYPIHENL
560 570 580 590 600
EHMAMMQRIN GTPFPTDIID KMFYKSKHKL GNSPSDLNST VIKHFDRKTL
610 620 630 640 650
SLQWPEKNKR GDTITTEKSM KRVLQSCDRL DIYISKVLKQ DYGDSLSINW
660 670 680 690 700
NLPPEKNWSL INSKLAWKRQ THSSSSSTTD ELDKETFLFW YWFIDLLRKM
710 720 730
FEFDPTKRIT AKDALDHEWF NLGILDDGIA TYNNTQG
Length:737
Mass (Da):83,843
Last modified:August 2, 2002 - v2
Checksum:iF0224445BDEE8431
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti411 – 421RFPGSHIQAIA → GSPALISGHC in AAA34724 (PubMed:1910150).CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85200 Genomic DNA. Translation: AAA34724.1.
Z73125 Genomic DNA. Translation: CAA97468.1.
X97560 Genomic DNA. Translation: CAA66171.1.
Z73123 Genomic DNA. Translation: CAA97465.1.
BK006945 Genomic DNA. Translation: DAA09300.1.
PIRiS64767.
RefSeqiNP_013081.1. NM_001181839.1.

Genome annotation databases

EnsemblFungiiYLL019C; YLL019C; YLL019C.
GeneIDi850641.
KEGGisce:YLL019C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85200 Genomic DNA. Translation: AAA34724.1.
Z73125 Genomic DNA. Translation: CAA97468.1.
X97560 Genomic DNA. Translation: CAA66171.1.
Z73123 Genomic DNA. Translation: CAA97465.1.
BK006945 Genomic DNA. Translation: DAA09300.1.
PIRiS64767.
RefSeqiNP_013081.1. NM_001181839.1.

3D structure databases

ProteinModelPortaliP32350.
SMRiP32350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31233. 137 interactors.
DIPiDIP-1900N.
IntActiP32350. 22 interactors.
MINTiMINT-396154.

PTM databases

iPTMnetiP32350.

Proteomic databases

PRIDEiP32350.
TopDownProteomicsiP32350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL019C; YLL019C; YLL019C.
GeneIDi850641.
KEGGisce:YLL019C.

Organism-specific databases

EuPathDBiFungiDB:YLL019C.
SGDiS000003942. KNS1.

Phylogenomic databases

GeneTreeiENSGT00580000081366.
HOGENOMiHOG000113240.
InParanoidiP32350.
KOiK08287.
OMAiEHMAMMQ.
OrthoDBiEOG092C2DKP.

Enzyme and pathway databases

BioCyciYEAST:G3O-32124-MONOMER.
BRENDAi2.7.12.1. 984.

Miscellaneous databases

PROiP32350.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 3 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKNS1_YEAST
AccessioniPrimary (citable) accession number: P32350
Secondary accession number(s): D6VXY4, Q12399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: August 2, 2002
Last modified: November 2, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.