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P32350

- KNS1_YEAST

UniProt

P32350 - KNS1_YEAST

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Protein
Dual specificity protein kinase KNS1
Gene
KNS1, YLL019C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Nonessential protein kinase.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei343 – 3431ATP By similarity
Active sitei440 – 4401Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi319 – 3279ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: SGD
  3. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
  4. protein tyrosine kinase activity Source: SGD

GO - Biological processi

  1. negative regulation of transcription from RNA polymerase III promoter Source: SGD
  2. peptidyl-tyrosine phosphorylation Source: GOC
  3. protein autophosphorylation Source: SGD
  4. protein phosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32124-MONOMER.
BRENDAi2.7.12.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase KNS1 (EC:2.7.12.1)
Gene namesi
Name:KNS1
Ordered Locus Names:YLL019C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLL019c.
SGDiS000003942. KNS1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 737737Dual specificity protein kinase KNS1
PRO_0000086155Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei562 – 5621Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylated (auto-) on Ser/Thr/Tyr.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP32350.
PeptideAtlasiP32350.
PRIDEiP32350.

Expressioni

Gene expression databases

GenevestigatoriP32350.

Interactioni

Protein-protein interaction databases

BioGridi31233. 68 interactions.
DIPiDIP-1900N.
IntActiP32350. 22 interactions.
MINTiMINT-396154.
STRINGi4932.YLL019C.

Structurei

3D structure databases

ProteinModelPortaliP32350.
SMRiP32350. Positions 294-648.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini313 – 720408Protein kinase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi143 – 1464Poly-Ser
Compositional biasi148 – 1525Poly-Asn
Compositional biasi673 – 6797Ser/Thr-rich

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000113240.
KOiK08287.
OMAiLYPIHEN.
OrthoDBiEOG7PGF0M.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 3 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32350-1 [UniParc]FASTAAdd to Basket

« Hide

MSQNIQIGTR KRSRANMNNS TTTGPANNTS SNKTFLDNFE ETRTNKLLDE    50
MFARQNSFLT DNLRNSLDLN QADNPLRPRQ HQHQLFLDNE NAIELDEEPR 100
IINTTINNSN NHNSSRVDED ADDDIIFIKE QPIQFSSPLI LPSSSSINNN 150
NNIVTSNNPG CGTAATSNST YITTPKKFKK QRTISLPQLP LSKLSYQSNY 200
FNVPDQTNAI VPRVTQTENE LLHLTGSCAK TLEGNKAVNL TIAHSTSPFS 250
NPPAQIASLP QSNLKKQIGS SLRKFTSNGS SESASSNKSN FKTDKDGHYV 300
YQENDIFGSG GRFVVKDLLG QGTFGKVLKC IDNKYEPNYV AVKVIRAVDR 350
YREAAKTELR ILQTILNNDP QGQFQCLLLR ECFDYKNHIC LVTDLYGRSI 400
YDFMCSNGIA RFPGSHIQAI ARQLIRSVCF LHDLGIIHTD LKPENILICD 450
ETHIAQKLPL KTVQSLSKRR REASKGKRKI LKNPEIKIID FGSAIFHYEY 500
HPPVISTRHY RAPEIVLGLG WSFPCDIWSI ACVLVELVIG ESLYPIHENL 550
EHMAMMQRIN GTPFPTDIID KMFYKSKHKL GNSPSDLNST VIKHFDRKTL 600
SLQWPEKNKR GDTITTEKSM KRVLQSCDRL DIYISKVLKQ DYGDSLSINW 650
NLPPEKNWSL INSKLAWKRQ THSSSSSTTD ELDKETFLFW YWFIDLLRKM 700
FEFDPTKRIT AKDALDHEWF NLGILDDGIA TYNNTQG 737
Length:737
Mass (Da):83,843
Last modified:August 2, 2002 - v2
Checksum:iF0224445BDEE8431
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti411 – 42111RFPGSHIQAIA → GSPALISGHC in AAA34724. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M85200 Genomic DNA. Translation: AAA34724.1.
Z73125 Genomic DNA. Translation: CAA97468.1.
X97560 Genomic DNA. Translation: CAA66171.1.
Z73123 Genomic DNA. Translation: CAA97465.1.
BK006945 Genomic DNA. Translation: DAA09300.1.
PIRiS64767.
RefSeqiNP_013081.1. NM_001181839.1.

Genome annotation databases

EnsemblFungiiYLL019C; YLL019C; YLL019C.
GeneIDi850641.
KEGGisce:YLL019C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M85200 Genomic DNA. Translation: AAA34724.1 .
Z73125 Genomic DNA. Translation: CAA97468.1 .
X97560 Genomic DNA. Translation: CAA66171.1 .
Z73123 Genomic DNA. Translation: CAA97465.1 .
BK006945 Genomic DNA. Translation: DAA09300.1 .
PIRi S64767.
RefSeqi NP_013081.1. NM_001181839.1.

3D structure databases

ProteinModelPortali P32350.
SMRi P32350. Positions 294-648.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31233. 68 interactions.
DIPi DIP-1900N.
IntActi P32350. 22 interactions.
MINTi MINT-396154.
STRINGi 4932.YLL019C.

Proteomic databases

PaxDbi P32350.
PeptideAtlasi P32350.
PRIDEi P32350.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLL019C ; YLL019C ; YLL019C .
GeneIDi 850641.
KEGGi sce:YLL019C.

Organism-specific databases

CYGDi YLL019c.
SGDi S000003942. KNS1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00580000081366.
HOGENOMi HOG000113240.
KOi K08287.
OMAi LYPIHEN.
OrthoDBi EOG7PGF0M.

Enzyme and pathway databases

BioCyci YEAST:G3O-32124-MONOMER.
BRENDAi 2.7.12.1. 984.

Miscellaneous databases

NextBioi 966573.

Gene expression databases

Genevestigatori P32350.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 3 hits.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The KNS1 gene of Saccharomyces cerevisiae encodes a nonessential protein kinase homologue that is distantly related to members of the CDC28/cdc2 gene family."
    Padmanabha R., Gehrung S., Snyder M.
    Mol. Gen. Genet. 229:1-9(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The sequence of 32kb on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABC transporter homologous to the human multidrug resistance protein."
    Purnelle B., Goffeau A.
    Yeast 13:183-188(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Activity and autophosphorylation of LAMMER protein kinases."
    Lee K., Du C., Horn M., Rabinow L.
    J. Biol. Chem. 271:27299-27303(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Negative regulation of filamentous growth and flocculation by Lkh1, a fission yeast LAMMER kinase homolog."
    Kim K.-H., Cho Y.-M., Kang W.-H., Kim J.-H., Byun K.-H., Park Y.-D., Bae K.-S., Park H.-M.
    Biochem. Biophys. Res. Commun. 289:1237-1242(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: AUTOPHOSPHORYLATION.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKNS1_YEAST
AccessioniPrimary (citable) accession number: P32350
Secondary accession number(s): D6VXY4, Q12399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: August 2, 2002
Last modified: June 11, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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