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P32350

- KNS1_YEAST

UniProt

P32350 - KNS1_YEAST

Protein

Dual specificity protein kinase KNS1

Gene

KNS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 2 (02 Aug 2002)
      Previous versions | rss
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    Functioni

    Nonessential protein kinase.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei343 – 3431ATPPROSITE-ProRule annotation
    Active sitei440 – 4401Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi319 – 3279ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
    3. protein serine/threonine kinase activity Source: SGD
    4. protein tyrosine kinase activity Source: SGD

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase III promoter Source: SGD
    2. peptidyl-tyrosine phosphorylation Source: GOC
    3. protein autophosphorylation Source: SGD
    4. protein phosphorylation Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32124-MONOMER.
    BRENDAi2.7.12.1. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein kinase KNS1 (EC:2.7.12.1)
    Gene namesi
    Name:KNS1
    Ordered Locus Names:YLL019C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLL019c.
    SGDiS000003942. KNS1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 737737Dual specificity protein kinase KNS1PRO_0000086155Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei562 – 5621Phosphothreonine1 Publication

    Post-translational modificationi

    Phosphorylated (auto-) on Ser/Thr/Tyr.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP32350.
    PeptideAtlasiP32350.
    PRIDEiP32350.

    Expressioni

    Gene expression databases

    GenevestigatoriP32350.

    Interactioni

    Protein-protein interaction databases

    BioGridi31233. 68 interactions.
    DIPiDIP-1900N.
    IntActiP32350. 22 interactions.
    MINTiMINT-396154.
    STRINGi4932.YLL019C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32350.
    SMRiP32350. Positions 294-648.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini313 – 720408Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi143 – 1464Poly-Ser
    Compositional biasi148 – 1525Poly-Asn
    Compositional biasi673 – 6797Ser/Thr-rich

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00580000081366.
    HOGENOMiHOG000113240.
    KOiK08287.
    OMAiLYPIHEN.
    OrthoDBiEOG7PGF0M.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 3 hits.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32350-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQNIQIGTR KRSRANMNNS TTTGPANNTS SNKTFLDNFE ETRTNKLLDE    50
    MFARQNSFLT DNLRNSLDLN QADNPLRPRQ HQHQLFLDNE NAIELDEEPR 100
    IINTTINNSN NHNSSRVDED ADDDIIFIKE QPIQFSSPLI LPSSSSINNN 150
    NNIVTSNNPG CGTAATSNST YITTPKKFKK QRTISLPQLP LSKLSYQSNY 200
    FNVPDQTNAI VPRVTQTENE LLHLTGSCAK TLEGNKAVNL TIAHSTSPFS 250
    NPPAQIASLP QSNLKKQIGS SLRKFTSNGS SESASSNKSN FKTDKDGHYV 300
    YQENDIFGSG GRFVVKDLLG QGTFGKVLKC IDNKYEPNYV AVKVIRAVDR 350
    YREAAKTELR ILQTILNNDP QGQFQCLLLR ECFDYKNHIC LVTDLYGRSI 400
    YDFMCSNGIA RFPGSHIQAI ARQLIRSVCF LHDLGIIHTD LKPENILICD 450
    ETHIAQKLPL KTVQSLSKRR REASKGKRKI LKNPEIKIID FGSAIFHYEY 500
    HPPVISTRHY RAPEIVLGLG WSFPCDIWSI ACVLVELVIG ESLYPIHENL 550
    EHMAMMQRIN GTPFPTDIID KMFYKSKHKL GNSPSDLNST VIKHFDRKTL 600
    SLQWPEKNKR GDTITTEKSM KRVLQSCDRL DIYISKVLKQ DYGDSLSINW 650
    NLPPEKNWSL INSKLAWKRQ THSSSSSTTD ELDKETFLFW YWFIDLLRKM 700
    FEFDPTKRIT AKDALDHEWF NLGILDDGIA TYNNTQG 737
    Length:737
    Mass (Da):83,843
    Last modified:August 2, 2002 - v2
    Checksum:iF0224445BDEE8431
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti411 – 42111RFPGSHIQAIA → GSPALISGHC in AAA34724. (PubMed:1910150)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85200 Genomic DNA. Translation: AAA34724.1.
    Z73125 Genomic DNA. Translation: CAA97468.1.
    X97560 Genomic DNA. Translation: CAA66171.1.
    Z73123 Genomic DNA. Translation: CAA97465.1.
    BK006945 Genomic DNA. Translation: DAA09300.1.
    PIRiS64767.
    RefSeqiNP_013081.1. NM_001181839.1.

    Genome annotation databases

    EnsemblFungiiYLL019C; YLL019C; YLL019C.
    GeneIDi850641.
    KEGGisce:YLL019C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85200 Genomic DNA. Translation: AAA34724.1 .
    Z73125 Genomic DNA. Translation: CAA97468.1 .
    X97560 Genomic DNA. Translation: CAA66171.1 .
    Z73123 Genomic DNA. Translation: CAA97465.1 .
    BK006945 Genomic DNA. Translation: DAA09300.1 .
    PIRi S64767.
    RefSeqi NP_013081.1. NM_001181839.1.

    3D structure databases

    ProteinModelPortali P32350.
    SMRi P32350. Positions 294-648.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31233. 68 interactions.
    DIPi DIP-1900N.
    IntActi P32350. 22 interactions.
    MINTi MINT-396154.
    STRINGi 4932.YLL019C.

    Proteomic databases

    PaxDbi P32350.
    PeptideAtlasi P32350.
    PRIDEi P32350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLL019C ; YLL019C ; YLL019C .
    GeneIDi 850641.
    KEGGi sce:YLL019C.

    Organism-specific databases

    CYGDi YLL019c.
    SGDi S000003942. KNS1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00580000081366.
    HOGENOMi HOG000113240.
    KOi K08287.
    OMAi LYPIHEN.
    OrthoDBi EOG7PGF0M.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32124-MONOMER.
    BRENDAi 2.7.12.1. 984.

    Miscellaneous databases

    NextBioi 966573.

    Gene expression databases

    Genevestigatori P32350.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 3 hits.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The KNS1 gene of Saccharomyces cerevisiae encodes a nonessential protein kinase homologue that is distantly related to members of the CDC28/cdc2 gene family."
      Padmanabha R., Gehrung S., Snyder M.
      Mol. Gen. Genet. 229:1-9(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The sequence of 32kb on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABC transporter homologous to the human multidrug resistance protein."
      Purnelle B., Goffeau A.
      Yeast 13:183-188(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Activity and autophosphorylation of LAMMER protein kinases."
      Lee K., Du C., Horn M., Rabinow L.
      J. Biol. Chem. 271:27299-27303(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Negative regulation of filamentous growth and flocculation by Lkh1, a fission yeast LAMMER kinase homolog."
      Kim K.-H., Cho Y.-M., Kang W.-H., Kim J.-H., Byun K.-H., Park Y.-D., Bae K.-S., Park H.-M.
      Biochem. Biophys. Res. Commun. 289:1237-1242(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: AUTOPHOSPHORYLATION.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-562, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKNS1_YEAST
    AccessioniPrimary (citable) accession number: P32350
    Secondary accession number(s): D6VXY4, Q12399
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: August 2, 2002
    Last modified: October 1, 2014
    This is version 139 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3