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Protein

Uroporphyrinogen decarboxylase

Gene

HEM12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.By similarity

Miscellaneous

Present with 9220 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Uroporphyrinogen III = coproporphyrinogen + 4 CO2.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (HEM2)
  2. Porphobilinogen deaminase (HEM3)
  3. Uroporphyrinogen-III synthase (HEM4)
  4. Uroporphyrinogen decarboxylase (HEM12)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48SubstrateBy similarity1
Binding sitei80SubstrateBy similarity1
Binding sitei81SubstrateBy similarity1
Sitei81Transition state stabilizerBy similarity1
Binding sitei160SubstrateBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei338SubstrateBy similarity1

GO - Molecular functioni

  • uroporphyrinogen decarboxylase activity Source: SGD

GO - Biological processi

  • heme biosynthetic process Source: SGD
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processHeme biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YDR047W-MONOMER
BRENDAi4.1.1.37 984
ReactomeiR-SCE-189451 Heme biosynthesis
UniPathwayiUPA00251; UER00321

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen decarboxylase (EC:4.1.1.37)
Short name:
UPD
Short name:
URO-D
Gene namesi
Name:HEM12
Synonyms:HEM6, POP3
Ordered Locus Names:YDR047W
ORF Names:YD9609.03
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR047W
SGDiS000002454 HEM12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33G → D: Inactivation. 1
Mutagenesisi300G → D: Inactivation. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001875771 – 362Uroporphyrinogen decarboxylaseAdd BLAST362

Proteomic databases

MaxQBiP32347
PaxDbiP32347
PRIDEiP32347

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399402EBI-5711,EBI-16219

Protein-protein interaction databases

BioGridi32102, 151 interactors
IntActiP32347, 3 interactors
MINTiP32347
STRINGi4932.YDR047W

Structurei

3D structure databases

ProteinModelPortaliP32347
SMRiP32347
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 34Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000018302
HOGENOMiHOG000253896
InParanoidiP32347
KOiK01599
OMAiSWAGQLS
OrthoDBiEOG092C2LLN

Family and domain databases

CDDicd00717 URO-D, 1 hit
Gene3Di3.20.20.210, 1 hit
HAMAPiMF_00218 URO_D, 1 hit
InterProiView protein in InterPro
IPR038071 UROD/MetE-like_sf
IPR006361 Uroporphyrinogen_deCO2ase_HemE
IPR000257 Uroporphyrinogen_deCOase
PfamiView protein in Pfam
PF01208 URO-D, 1 hit
SUPFAMiSSF51726 SSF51726, 1 hit
TIGRFAMsiTIGR01464 hemE, 1 hit
PROSITEiView protein in PROSITE
PS00906 UROD_1, 1 hit
PS00907 UROD_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32347-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNFPAPKND LILRAAKGEK VERPPCWIMR QAGRYLPEYH EVKNNRDFFQ
60 70 80 90 100
TCRDAEIASE ITIQPVRRYR GLIDAAIIFS DILVIPQAMG MRVEMLEGKG
110 120 130 140 150
PHFPEPLRNP EDLQTVLDYK VDVLKELDWA FKAITMTRIK LDGEVPLFGF
160 170 180 190 200
CGGPWTLMVY MTEGGGSRLF RFAKQWINMY PELSHKLLQK ITDVAVEFLS
210 220 230 240 250
QQVVAGAQIL QVFESWGGEL SSVDFDEFSL PYLRQIAERV PKRLQELGIM
260 270 280 290 300
EQIPMIVFAK GSWYALDKLC CSGFDVVSLD WSWDPREAVK INKNRVTLQG
310 320 330 340 350
NLDPGVMYGS KEVITKKVKQ MIEAFGGGKS RYIVNFGHGT HPFMDPDVIK
360
FFLEECHRIG SK
Length:362
Mass (Da):41,349
Last modified:October 1, 1993 - v1
Checksum:iE9CB3A48E62BC277
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti59S → F in HEM12-6 and HEM12-12. 1
Natural varianti62T → I in HEM12-14. 1
Natural varianti107L → S in HEM12-3 and HEM12-13. 1
Natural varianti215S → N in HEM12-2 and HEM12-11. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63721 Genomic DNA Translation: CAA45253.1
Z19089 Genomic DNA Translation: CAA79514.1
Z49209 Genomic DNA Translation: CAA89078.1
BK006938 Genomic DNA Translation: DAA11895.1
PIRiS23471
RefSeqiNP_010332.3, NM_001180355.3

Genome annotation databases

EnsemblFungiiYDR047W; YDR047W; YDR047W
GeneIDi851617
KEGGisce:YDR047W

Similar proteinsi

Entry informationi

Entry nameiDCUP_YEAST
AccessioniPrimary (citable) accession number: P32347
Secondary accession number(s): D6VS35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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