Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P32345 (PP4C_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit

Short name=PP4C
EC=3.1.3.16
Alternative name(s):
Phosphatase PP2A-like catalytic subunit PPH3
Gene names
Name:PPH3
Ordered Locus Names:YDR075W
ORF Names:D4421
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms the histone H2A phosphatase complex in association with the regulatory subunits PSY2 and PSY4, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. PPH3 is directly involved in the dephosphorylation and activation of the transcription factor GLN3 in response to nutrient availability. Ref.6 Ref.12

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Subunit structure

Catalytic subunit of the histone H2A phosphatase complex (HTP-C) containing PPH3, PSY2 and PSY4. Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with SPT5 and TAP42. Ref.7 Ref.10 Ref.11 Ref.12

Subcellular location

Cytoplasm. Nucleus Ref.8.

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PSY4P381938EBI-12759,EBI-21239

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308Serine/threonine-protein phosphatase 4 catalytic subunit
PRO_0000058875

Sites

Active site1121Proton donor By similarity
Metal binding511Iron By similarity
Metal binding531Iron By similarity
Metal binding791Iron By similarity
Metal binding791Manganese By similarity
Metal binding1111Manganese By similarity
Metal binding1611Manganese By similarity
Metal binding2351Manganese By similarity

Experimental info

Sequence conflict2451M → I in CAA41662. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32345 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: AFE99582C7F44052

FASTA30835,264
        10         20         30         40         50         60 
MMDLDKIIAS LRDGKHIPEE TVFRLCLNSQ ELLMNEGNVT QVDTPVTICG DIHGQLHDLL 

        70         80         90        100        110        120 
TLFEKSGGVE KTRYIFLGDF VDRGFYSLES FLLLLCYKLR YPDRITLIRG NHETRQITKV 

       130        140        150        160        170        180 
YGFYDEVVRK YGNSNVWRYC CEVFDYLSLG AIINNSIFCV HGGLSPDMTT VDEIRTIDRK 

       190        200        210        220        230        240 
QEVPHEGAMC DLLWSDPEDV DTWSLSPRGA GFLFGKREVD QFLEKNNVEL IARAHQLVME 

       250        260        270        280        290        300 
GYKEMFDGGL VTVWSAPNYC YRCGNVAAVL KIDDDLNREY TIFEAVQAQN EVGNAIIPTK 


KSQMDYFL 

« Hide

References

« Hide 'large scale' references
[1]"Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis."
Ronne H., Carlberg M., Hu G.-Z., Nehlin J.O.
Mol. Cell. Biol. 11:4876-4884(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 208353 / W303-1A.
[2]"The Saccharomyces cerevisiae gene PPH3 encodes a protein phosphatase with properties different from PPX, PP1 and PP2A."
Hoffmann R., Jung S., Ehrmann M., Hofer H.W.
Yeast 10:567-578(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Tripartite regulation of Gln3p by TOR, Ure2p, and phosphatases."
Bertram P.G., Choi J.H., Carvalho J., Ai W., Zeng C., Chan T.-F., Zheng X.F.S.
J. Biol. Chem. 275:35727-35733(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN GLN3 DEPHOSPHORYLATION.
[7]"Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases."
Wang H., Wang X., Jiang Y.
Mol. Biol. Cell 14:4342-4351(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TAP42.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PPE1 AND RRD1.
[11]"A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity."
Gingras A.-C., Caballero M., Zarske M., Sanchez A., Hazbun T.R., Fields S., Sonenberg N., Hafen E., Raught B., Aebersold R.
Mol. Cell. Proteomics 4:1725-1740(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN HTP-C COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH SPT5.
[12]"A phosphatase complex that dephosphorylates gamma-H2AX regulates DNA damage checkpoint recovery."
Keogh M.-C., Kim J.-A., Downey M., Fillingham J., Chowdhury D., Harrison J.C., Onishi M., Datta N., Galicia S., Emili A., Lieberman J., Shen X., Buratowski S., Haber J.E., Durocher D., Greenblatt J.F., Krogan N.J.
Nature 439:497-501(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN HTP-C COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46796 Genomic DNA. Translation: CAA86797.1.
X58858 Genomic DNA. Translation: CAA41662.1.
X82086 Genomic DNA. Translation: CAA57602.1.
Z74371 Genomic DNA. Translation: CAA98894.1.
AY557686 Genomic DNA. Translation: AAS56012.1.
BK006938 Genomic DNA. Translation: DAA11921.1.
PIRPABY3. S44331.
RefSeqNP_010360.1. NM_001180383.1.

3D structure databases

ProteinModelPortalP32345.
SMRP32345. Positions 3-284.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32130. 174 interactions.
DIPDIP-3905N.
IntActP32345. 8 interactions.
MINTMINT-509529.
STRING4932.YDR075W.

Proteomic databases

PaxDbP32345.
PeptideAtlasP32345.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR075W; YDR075W; YDR075W.
GeneID851647.
KEGGsce:YDR075W.

Organism-specific databases

CYGDYDR075w.
SGDS000002482. PPH3.

Phylogenomic databases

eggNOGCOG0639.
GeneTreeENSGT00710000107291.
HOGENOMHOG000172696.
KOK15423.
OMAVEEDIFC.
OrthoDBEOG7FFN29.

Enzyme and pathway databases

BioCycYEAST:G3O-29680-MONOMER.

Gene expression databases

GenevestigatorP32345.

Family and domain databases

InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio969227.
PROP32345.

Entry information

Entry namePP4C_YEAST
AccessionPrimary (citable) accession number: P32345
Secondary accession number(s): D6VS61
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families