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P32345

- PP4C_YEAST

UniProt

P32345 - PP4C_YEAST

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Protein
Serine/threonine-protein phosphatase 4 catalytic subunit
Gene
PPH3, YDR075W, D4421
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Forms the histone H2A phosphatase complex in association with the regulatory subunits PSY2 and PSY4, which dephosphorylates H2AS128ph (gamma-H2A) that has been displaced from sites of DNA lesions in the double-stranded DNA break repair process. Dephosphorylation is necessary for efficient recovery from the DNA damage checkpoint. PPH3 is directly involved in the dephosphorylation and activation of the transcription factor GLN3 in response to nutrient availability.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511Manganese 1 By similarity
Metal bindingi53 – 531Manganese 1 By similarity
Metal bindingi79 – 791Manganese 1 By similarity
Metal bindingi79 – 791Manganese 2 By similarity
Metal bindingi111 – 1111Manganese 2 By similarity
Active sitei112 – 1121Proton donor By similarity
Metal bindingi161 – 1611Manganese 2 By similarity
Metal bindingi235 – 2351Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  1. double-strand break repair via homologous recombination Source: SGD
  2. negative regulation of DNA damage checkpoint Source: SGD
  3. negative regulation of glucose mediated signaling pathway Source: SGD
  4. positive regulation of nitrogen compound metabolic process Source: SGD
  5. protein dephosphorylation Source: SGD
  6. signal transduction involved in meiotic recombination checkpoint Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29680-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
Short name:
PP4C
Alternative name(s):
Phosphatase PP2A-like catalytic subunit PPH3
Gene namesi
Name:PPH3
Ordered Locus Names:YDR075W
ORF Names:D4421
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR075w.
SGDiS000002482. PPH3.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. protein phosphatase 4 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Serine/threonine-protein phosphatase 4 catalytic subunit
PRO_0000058875Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei308 – 3081Leucine methyl ester By similarity

Post-translational modificationi

Reversibly methyl esterified on Leu-308 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization By similarity.

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP32345.
PaxDbiP32345.
PeptideAtlasiP32345.

Expressioni

Gene expression databases

GenevestigatoriP32345.

Interactioni

Subunit structurei

Catalytic subunit of the histone H2A phosphatase complex (HTP-C) containing PPH3, PSY2 and PSY4. Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD1, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with SPT5 and TAP42.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PSY4P381938EBI-12759,EBI-21239

Protein-protein interaction databases

BioGridi32130. 210 interactions.
DIPiDIP-3905N.
IntActiP32345. 8 interactions.
MINTiMINT-509529.
STRINGi4932.YDR075W.

Structurei

3D structure databases

ProteinModelPortaliP32345.
SMRiP32345. Positions 3-284.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00710000107291.
HOGENOMiHOG000172696.
KOiK15423.
OMAiVEEDIFC.
OrthoDBiEOG7FFN29.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32345-1 [UniParc]FASTAAdd to Basket

« Hide

MMDLDKIIAS LRDGKHIPEE TVFRLCLNSQ ELLMNEGNVT QVDTPVTICG    50
DIHGQLHDLL TLFEKSGGVE KTRYIFLGDF VDRGFYSLES FLLLLCYKLR 100
YPDRITLIRG NHETRQITKV YGFYDEVVRK YGNSNVWRYC CEVFDYLSLG 150
AIINNSIFCV HGGLSPDMTT VDEIRTIDRK QEVPHEGAMC DLLWSDPEDV 200
DTWSLSPRGA GFLFGKREVD QFLEKNNVEL IARAHQLVME GYKEMFDGGL 250
VTVWSAPNYC YRCGNVAAVL KIDDDLNREY TIFEAVQAQN EVGNAIIPTK 300
KSQMDYFL 308
Length:308
Mass (Da):35,264
Last modified:February 1, 1995 - v2
Checksum:iAFE99582C7F44052
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti245 – 2451M → I in CAA41662. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46796 Genomic DNA. Translation: CAA86797.1.
X58858 Genomic DNA. Translation: CAA41662.1.
X82086 Genomic DNA. Translation: CAA57602.1.
Z74371 Genomic DNA. Translation: CAA98894.1.
AY557686 Genomic DNA. Translation: AAS56012.1.
BK006938 Genomic DNA. Translation: DAA11921.1.
PIRiS44331. PABY3.
RefSeqiNP_010360.1. NM_001180383.1.

Genome annotation databases

EnsemblFungiiYDR075W; YDR075W; YDR075W.
GeneIDi851647.
KEGGisce:YDR075W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z46796 Genomic DNA. Translation: CAA86797.1 .
X58858 Genomic DNA. Translation: CAA41662.1 .
X82086 Genomic DNA. Translation: CAA57602.1 .
Z74371 Genomic DNA. Translation: CAA98894.1 .
AY557686 Genomic DNA. Translation: AAS56012.1 .
BK006938 Genomic DNA. Translation: DAA11921.1 .
PIRi S44331. PABY3.
RefSeqi NP_010360.1. NM_001180383.1.

3D structure databases

ProteinModelPortali P32345.
SMRi P32345. Positions 3-284.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32130. 210 interactions.
DIPi DIP-3905N.
IntActi P32345. 8 interactions.
MINTi MINT-509529.
STRINGi 4932.YDR075W.

Proteomic databases

MaxQBi P32345.
PaxDbi P32345.
PeptideAtlasi P32345.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR075W ; YDR075W ; YDR075W .
GeneIDi 851647.
KEGGi sce:YDR075W.

Organism-specific databases

CYGDi YDR075w.
SGDi S000002482. PPH3.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00710000107291.
HOGENOMi HOG000172696.
KOi K15423.
OMAi VEEDIFC.
OrthoDBi EOG7FFN29.

Enzyme and pathway databases

BioCyci YEAST:G3O-29680-MONOMER.

Miscellaneous databases

NextBioi 969227.
PROi P32345.

Gene expression databases

Genevestigatori P32345.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis."
    Ronne H., Carlberg M., Hu G.-Z., Nehlin J.O.
    Mol. Cell. Biol. 11:4876-4884(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 208353 / W303-1A.
  2. "The Saccharomyces cerevisiae gene PPH3 encodes a protein phosphatase with properties different from PPX, PP1 and PP2A."
    Hoffmann R., Jung S., Ehrmann M., Hofer H.W.
    Yeast 10:567-578(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Tripartite regulation of Gln3p by TOR, Ure2p, and phosphatases."
    Bertram P.G., Choi J.H., Carvalho J., Ai W., Zeng C., Chan T.-F., Zheng X.F.S.
    J. Biol. Chem. 275:35727-35733(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GLN3 DEPHOSPHORYLATION.
  7. "Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases."
    Wang H., Wang X., Jiang Y.
    Mol. Biol. Cell 14:4342-4351(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TAP42.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
    Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
    Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPE1 AND RRD1.
  11. "A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity."
    Gingras A.-C., Caballero M., Zarske M., Sanchez A., Hazbun T.R., Fields S., Sonenberg N., Hafen E., Raught B., Aebersold R.
    Mol. Cell. Proteomics 4:1725-1740(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN HTP-C COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH SPT5.
  12. Cited for: FUNCTION, IDENTIFICATION IN HTP-C COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPP4C_YEAST
AccessioniPrimary (citable) accession number: P32345
Secondary accession number(s): D6VS61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi