Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P32343

- SSH4_YEAST

UniProt

P32343 - SSH4_YEAST

Protein

Protein SSH4

Gene

SSH4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Components of the endosome-vacuole trafficking pathway that regulates nutrient transport. May be involved in processes which determine whether plasma membrane proteins are degraded or routed to the plasma membrane. Confers leflunomide resistance when overexpressed.2 Publications

    GO - Biological processi

    1. protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
    2. vesicle-mediated transport Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31906-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein SSH4
    Alternative name(s):
    Multicopy suppressor of leflunomide protein 4
    Suppressor of SHR3 null mutation protein 4
    Gene namesi
    Name:SSH4
    Synonyms:MLF4
    Ordered Locus Names:YKL124W
    ORF Names:YKL529
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL124w.
    SGDiS000001607. SSH4.

    Subcellular locationi

    Vacuole membrane 1 Publication; Single-pass type II membrane protein 1 Publication. Endosome membrane 1 Publication; Single-pass type II membrane protein 1 Publication

    GO - Cellular componenti

    1. endosome membrane Source: UniProtKB-SubCell
    2. fungal-type vacuole Source: SGD
    3. integral component of membrane Source: UniProtKB-KW
    4. vacuolar membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endosome, Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 579579Protein SSH4PRO_0000203153Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
    Modified residuei358 – 3581Phosphoserine3 Publications
    Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi507 – 5071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi557 – 5571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi575 – 5751N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP32343.
    PaxDbiP32343.

    Expressioni

    Gene expression databases

    GenevestigatoriP32343.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RSP5P399401EBI-26858,EBI-16219

    Protein-protein interaction databases

    BioGridi34012. 16 interactions.
    DIPiDIP-5420N.
    IntActiP32343. 1 interaction.
    MINTiMINT-480218.
    STRINGi4932.YKL124W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32343.
    SMRiP32343. Positions 198-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4444CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini66 – 579514LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei45 – 6521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini166 – 364199B30.2/SPRYPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi490 – 55768Asn-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SSH4 family.Curated
    Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG278737.
    GeneTreeiENSGT00530000067969.
    HOGENOMiHOG000154429.
    OMAiIFITHNG.
    OrthoDBiEOG7T1RKP.

    Family and domain databases

    InterProiIPR001870. B30.2/SPRY.
    IPR008985. ConA-like_lec_gl_sf.
    IPR018355. SPla/RYanodine_receptor_subgr.
    IPR003877. SPRY_rcpt.
    [Graphical view]
    PfamiPF00622. SPRY. 1 hit.
    [Graphical view]
    SMARTiSM00449. SPRY. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS50188. B302_SPRY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32343-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYVTFNEALD SSFGNLESPN HDFKVGDPNM VPTPPMDSDS AAISLAFLIS    50
    LSITFAILML ILVVIAAYVT FCGDDESEYD EENALGTRTS GTLHSLFGKK 100
    HSGILLDSSF ASPGGFDDEI VLQERELEEL PKMSAYEVEL YIRAKEFQMM 150
    SPPMVKDFGT YLDSDDQQFI KDRGIQSYFL LPSINDNIDE YGNFLPSFIV 200
    QDKLDIQFSK FNKSSSTVMN YPLPHNRKDA VYFEVKIFRH IQKSNSIFSI 250
    GLTTVPYPYF RVPGMAKYSI AYESTGKLRI NNPFTASTLL PKLEEGDTVG 300
    FGYRYKTGTI FITHNGKKLM DVTQNIGIDL FIGIGAFNAA YTRTYTRDGL 350
    LEDPDNVSFR EALSEGKDIE VAKDLQRVHD PHDESDEMTS DEVELHVNLG 400
    QVGFVFIEAN VKKYAFGSVY GQIGIPPAYN GTEIKKDTIL QKGEELPPRY 450
    ADTDNFFGSM KVKEGSSSRI TAQTSKPLWS VGTYERISSN FDRENNVYHD 500
    SLETDDNNTD NNVNNNDENA GCNENSPLLE DDGNKRPENS NTPREVSDGA 550
    INKNPRNKST KKRQRNRGKS SKKKNRSRK 579
    Length:579
    Mass (Da):65,061
    Last modified:June 1, 1994 - v2
    Checksum:i700F1CBA07A49754
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28123 Genomic DNA. Translation: CAA81964.1.
    S44213 Genomic DNA. Translation: AAB23074.1.
    BK006944 Genomic DNA. Translation: DAA09037.1.
    PIRiS37953.
    RefSeqiNP_012798.1. NM_001179690.1.

    Genome annotation databases

    EnsemblFungiiYKL124W; YKL124W; YKL124W.
    GeneIDi853735.
    KEGGisce:YKL124W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28123 Genomic DNA. Translation: CAA81964.1 .
    S44213 Genomic DNA. Translation: AAB23074.1 .
    BK006944 Genomic DNA. Translation: DAA09037.1 .
    PIRi S37953.
    RefSeqi NP_012798.1. NM_001179690.1.

    3D structure databases

    ProteinModelPortali P32343.
    SMRi P32343. Positions 198-339.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34012. 16 interactions.
    DIPi DIP-5420N.
    IntActi P32343. 1 interaction.
    MINTi MINT-480218.
    STRINGi 4932.YKL124W.

    Proteomic databases

    MaxQBi P32343.
    PaxDbi P32343.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL124W ; YKL124W ; YKL124W .
    GeneIDi 853735.
    KEGGi sce:YKL124W.

    Organism-specific databases

    CYGDi YKL124w.
    SGDi S000001607. SSH4.

    Phylogenomic databases

    eggNOGi NOG278737.
    GeneTreei ENSGT00530000067969.
    HOGENOMi HOG000154429.
    OMAi IFITHNG.
    OrthoDBi EOG7T1RKP.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31906-MONOMER.

    Miscellaneous databases

    NextBioi 974781.

    Gene expression databases

    Genevestigatori P32343.

    Family and domain databases

    InterProi IPR001870. B30.2/SPRY.
    IPR008985. ConA-like_lec_gl_sf.
    IPR018355. SPla/RYanodine_receptor_subgr.
    IPR003877. SPRY_rcpt.
    [Graphical view ]
    Pfami PF00622. SPRY. 1 hit.
    [Graphical view ]
    SMARTi SM00449. SPRY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS50188. B302_SPRY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Sequence of a segment of yeast chromosome XI identifies a new mitochondrial carrier, a new member of the G protein family, and a protein with the PAAKK motif of the H1 histones."
      Colleaux L., Richard G.-F., Thierry A., Dujon B.
      Yeast 8:325-336(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 41-579.
    4. "Molecular cloning of Saccharomyces cerevisiae MLF4/SSH4 gene which confers the immunosuppressant leflunomide resistance."
      Fujimura H.
      Biochem. Biophys. Res. Commun. 246:378-381(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Ssh4, Rcr2 and Rcr1 affect plasma membrane transporter activity in Saccharomyces cerevisiae."
      Kota J., Melin-Larsson M., Ljungdahl P.O., Forsberg H.
      Genetics 175:1681-1694(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSSH4_YEAST
    AccessioniPrimary (citable) accession number: P32343
    Secondary accession number(s): D6VX71
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 450 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3