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Protein

Protein SSH4

Gene

SSH4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Components of the endosome-vacuole trafficking pathway that regulates nutrient transport. May be involved in processes which determine whether plasma membrane proteins are degraded or routed to the plasma membrane. Confers leflunomide resistance when overexpressed.2 Publications

GO - Biological processi

  • protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31906-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SSH4
Alternative name(s):
Multicopy suppressor of leflunomide protein 4
Suppressor of SHR3 null mutation protein 4
Gene namesi
Name:SSH4
Synonyms:MLF4
Ordered Locus Names:YKL124W
ORF Names:YKL529
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL124W.
SGDiS000001607. SSH4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 44CytoplasmicSequence analysisAdd BLAST44
Transmembranei45 – 65Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini66 – 579LumenalSequence analysisAdd BLAST514

GO - Cellular componenti

  • endosome membrane Source: UniProtKB-SubCell
  • fungal-type vacuole Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002031531 – 579Protein SSH4Add BLAST579

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi212N-linked (GlcNAc...)Sequence analysis1
Glycosylationi356N-linked (GlcNAc...)Sequence analysis1
Modified residuei358PhosphoserineCombined sources1
Cross-linki367Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Glycosylationi430N-linked (GlcNAc...)Sequence analysis1
Glycosylationi507N-linked (GlcNAc...)Sequence analysis1
Glycosylationi557N-linked (GlcNAc...)Sequence analysis1
Glycosylationi575N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP32343.
PRIDEiP32343.

PTM databases

iPTMnetiP32343.

Interactioni

Protein-protein interaction databases

BioGridi34012. 18 interactors.
DIPiDIP-5420N.
IntActiP32343. 1 interactor.
MINTiMINT-480218.

Structurei

3D structure databases

ProteinModelPortaliP32343.
SMRiP32343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini166 – 364B30.2/SPRYPROSITE-ProRule annotationAdd BLAST199

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi490 – 557Asn-richAdd BLAST68

Sequence similaritiesi

Belongs to the SSH4 family.Curated
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000067969.
HOGENOMiHOG000154429.
InParanoidiP32343.
OMAiIFITHNG.
OrthoDBiEOG092C16NT.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYVTFNEALD SSFGNLESPN HDFKVGDPNM VPTPPMDSDS AAISLAFLIS
60 70 80 90 100
LSITFAILML ILVVIAAYVT FCGDDESEYD EENALGTRTS GTLHSLFGKK
110 120 130 140 150
HSGILLDSSF ASPGGFDDEI VLQERELEEL PKMSAYEVEL YIRAKEFQMM
160 170 180 190 200
SPPMVKDFGT YLDSDDQQFI KDRGIQSYFL LPSINDNIDE YGNFLPSFIV
210 220 230 240 250
QDKLDIQFSK FNKSSSTVMN YPLPHNRKDA VYFEVKIFRH IQKSNSIFSI
260 270 280 290 300
GLTTVPYPYF RVPGMAKYSI AYESTGKLRI NNPFTASTLL PKLEEGDTVG
310 320 330 340 350
FGYRYKTGTI FITHNGKKLM DVTQNIGIDL FIGIGAFNAA YTRTYTRDGL
360 370 380 390 400
LEDPDNVSFR EALSEGKDIE VAKDLQRVHD PHDESDEMTS DEVELHVNLG
410 420 430 440 450
QVGFVFIEAN VKKYAFGSVY GQIGIPPAYN GTEIKKDTIL QKGEELPPRY
460 470 480 490 500
ADTDNFFGSM KVKEGSSSRI TAQTSKPLWS VGTYERISSN FDRENNVYHD
510 520 530 540 550
SLETDDNNTD NNVNNNDENA GCNENSPLLE DDGNKRPENS NTPREVSDGA
560 570
INKNPRNKST KKRQRNRGKS SKKKNRSRK
Length:579
Mass (Da):65,061
Last modified:June 1, 1994 - v2
Checksum:i700F1CBA07A49754
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28123 Genomic DNA. Translation: CAA81964.1.
S44213 Genomic DNA. Translation: AAB23074.1.
BK006944 Genomic DNA. Translation: DAA09037.1.
PIRiS37953.
RefSeqiNP_012798.1. NM_001179690.1.

Genome annotation databases

EnsemblFungiiYKL124W; YKL124W; YKL124W.
GeneIDi853735.
KEGGisce:YKL124W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28123 Genomic DNA. Translation: CAA81964.1.
S44213 Genomic DNA. Translation: AAB23074.1.
BK006944 Genomic DNA. Translation: DAA09037.1.
PIRiS37953.
RefSeqiNP_012798.1. NM_001179690.1.

3D structure databases

ProteinModelPortaliP32343.
SMRiP32343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34012. 18 interactors.
DIPiDIP-5420N.
IntActiP32343. 1 interactor.
MINTiMINT-480218.

PTM databases

iPTMnetiP32343.

Proteomic databases

MaxQBiP32343.
PRIDEiP32343.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL124W; YKL124W; YKL124W.
GeneIDi853735.
KEGGisce:YKL124W.

Organism-specific databases

EuPathDBiFungiDB:YKL124W.
SGDiS000001607. SSH4.

Phylogenomic databases

GeneTreeiENSGT00530000067969.
HOGENOMiHOG000154429.
InParanoidiP32343.
OMAiIFITHNG.
OrthoDBiEOG092C16NT.

Enzyme and pathway databases

BioCyciYEAST:G3O-31906-MONOMER.

Miscellaneous databases

PROiP32343.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSH4_YEAST
AccessioniPrimary (citable) accession number: P32343
Secondary accession number(s): D6VX71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 450 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.