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P32343

- SSH4_YEAST

UniProt

P32343 - SSH4_YEAST

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Protein
Protein SSH4
Gene
SSH4, MLF4, YKL124W, YKL529
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Components of the endosome-vacuole trafficking pathway that regulates nutrient transport. May be involved in processes which determine whether plasma membrane proteins are degraded or routed to the plasma membrane. Confers leflunomide resistance when overexpressed.2 Publications

GO - Biological processi

  1. protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
  2. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31906-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SSH4
Alternative name(s):
Multicopy suppressor of leflunomide protein 4
Suppressor of SHR3 null mutation protein 4
Gene namesi
Name:SSH4
Synonyms:MLF4
Ordered Locus Names:YKL124W
ORF Names:YKL529
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL124w.
SGDiS000001607. SSH4.

Subcellular locationi

Vacuole membrane; Single-pass type II membrane protein. Endosome membrane; Single-pass type II membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4444Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei45 – 6521Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini66 – 579514Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endosome membrane Source: UniProtKB-SubCell
  2. fungal-type vacuole Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
  4. vacuolar membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 579579Protein SSH4
PRO_0000203153Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi212 – 2121N-linked (GlcNAc...) Reviewed prediction
Glycosylationi356 – 3561N-linked (GlcNAc...) Reviewed prediction
Modified residuei358 – 3581Phosphoserine3 Publications
Glycosylationi430 – 4301N-linked (GlcNAc...) Reviewed prediction
Glycosylationi507 – 5071N-linked (GlcNAc...) Reviewed prediction
Glycosylationi557 – 5571N-linked (GlcNAc...) Reviewed prediction
Glycosylationi575 – 5751N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32343.
PaxDbiP32343.

Expressioni

Gene expression databases

GenevestigatoriP32343.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RSP5P399401EBI-26858,EBI-16219

Protein-protein interaction databases

BioGridi34012. 16 interactions.
DIPiDIP-5420N.
IntActiP32343. 1 interaction.
MINTiMINT-480218.
STRINGi4932.YKL124W.

Structurei

3D structure databases

ProteinModelPortaliP32343.
SMRiP32343. Positions 198-339.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini166 – 364199B30.2/SPRY
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi490 – 55768Asn-rich
Add
BLAST

Sequence similaritiesi

Belongs to the SSH4 family.
Contains 1 B30.2/SPRY domain.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG278737.
GeneTreeiENSGT00530000067969.
HOGENOMiHOG000154429.
OMAiIFITHNG.
OrthoDBiEOG7T1RKP.

Family and domain databases

InterProiIPR001870. B30.2/SPRY.
IPR008985. ConA-like_lec_gl_sf.
IPR018355. SPla/RYanodine_receptor_subgr.
IPR003877. SPRY_rcpt.
[Graphical view]
PfamiPF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32343-1 [UniParc]FASTAAdd to Basket

« Hide

MYVTFNEALD SSFGNLESPN HDFKVGDPNM VPTPPMDSDS AAISLAFLIS    50
LSITFAILML ILVVIAAYVT FCGDDESEYD EENALGTRTS GTLHSLFGKK 100
HSGILLDSSF ASPGGFDDEI VLQERELEEL PKMSAYEVEL YIRAKEFQMM 150
SPPMVKDFGT YLDSDDQQFI KDRGIQSYFL LPSINDNIDE YGNFLPSFIV 200
QDKLDIQFSK FNKSSSTVMN YPLPHNRKDA VYFEVKIFRH IQKSNSIFSI 250
GLTTVPYPYF RVPGMAKYSI AYESTGKLRI NNPFTASTLL PKLEEGDTVG 300
FGYRYKTGTI FITHNGKKLM DVTQNIGIDL FIGIGAFNAA YTRTYTRDGL 350
LEDPDNVSFR EALSEGKDIE VAKDLQRVHD PHDESDEMTS DEVELHVNLG 400
QVGFVFIEAN VKKYAFGSVY GQIGIPPAYN GTEIKKDTIL QKGEELPPRY 450
ADTDNFFGSM KVKEGSSSRI TAQTSKPLWS VGTYERISSN FDRENNVYHD 500
SLETDDNNTD NNVNNNDENA GCNENSPLLE DDGNKRPENS NTPREVSDGA 550
INKNPRNKST KKRQRNRGKS SKKKNRSRK 579
Length:579
Mass (Da):65,061
Last modified:June 1, 1994 - v2
Checksum:i700F1CBA07A49754
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z28123 Genomic DNA. Translation: CAA81964.1.
S44213 Genomic DNA. Translation: AAB23074.1.
BK006944 Genomic DNA. Translation: DAA09037.1.
PIRiS37953.
RefSeqiNP_012798.1. NM_001179690.1.

Genome annotation databases

EnsemblFungiiYKL124W; YKL124W; YKL124W.
GeneIDi853735.
KEGGisce:YKL124W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z28123 Genomic DNA. Translation: CAA81964.1 .
S44213 Genomic DNA. Translation: AAB23074.1 .
BK006944 Genomic DNA. Translation: DAA09037.1 .
PIRi S37953.
RefSeqi NP_012798.1. NM_001179690.1.

3D structure databases

ProteinModelPortali P32343.
SMRi P32343. Positions 198-339.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34012. 16 interactions.
DIPi DIP-5420N.
IntActi P32343. 1 interaction.
MINTi MINT-480218.
STRINGi 4932.YKL124W.

Proteomic databases

MaxQBi P32343.
PaxDbi P32343.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL124W ; YKL124W ; YKL124W .
GeneIDi 853735.
KEGGi sce:YKL124W.

Organism-specific databases

CYGDi YKL124w.
SGDi S000001607. SSH4.

Phylogenomic databases

eggNOGi NOG278737.
GeneTreei ENSGT00530000067969.
HOGENOMi HOG000154429.
OMAi IFITHNG.
OrthoDBi EOG7T1RKP.

Enzyme and pathway databases

BioCyci YEAST:G3O-31906-MONOMER.

Miscellaneous databases

NextBioi 974781.

Gene expression databases

Genevestigatori P32343.

Family and domain databases

InterProi IPR001870. B30.2/SPRY.
IPR008985. ConA-like_lec_gl_sf.
IPR018355. SPla/RYanodine_receptor_subgr.
IPR003877. SPRY_rcpt.
[Graphical view ]
Pfami PF00622. SPRY. 1 hit.
[Graphical view ]
SMARTi SM00449. SPRY. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS50188. B302_SPRY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Sequence of a segment of yeast chromosome XI identifies a new mitochondrial carrier, a new member of the G protein family, and a protein with the PAAKK motif of the H1 histones."
    Colleaux L., Richard G.-F., Thierry A., Dujon B.
    Yeast 8:325-336(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 41-579.
  4. "Molecular cloning of Saccharomyces cerevisiae MLF4/SSH4 gene which confers the immunosuppressant leflunomide resistance."
    Fujimura H.
    Biochem. Biophys. Res. Commun. 246:378-381(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Ssh4, Rcr2 and Rcr1 affect plasma membrane transporter activity in Saccharomyces cerevisiae."
    Kota J., Melin-Larsson M., Ljungdahl P.O., Forsberg H.
    Genetics 175:1681-1694(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSSH4_YEAST
AccessioniPrimary (citable) accession number: P32343
Secondary accession number(s): D6VX71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 450 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi