P32341 (VPH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar ATPase assembly integral membrane protein VPH2

Alternative name(s):
Protein VMA12

Gene names

Name:	VPH2
Synonyms:	CLS10, VMA12
Ordered Locus Names:	YKL119C
ORF Names:	YKL520

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for vacuolar ATPase assembly.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.7.
Miscellaneous	Present with 2030 molecules/cell in log phase SD medium. Ref.8

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	vacuolar acidification Inferred from mutant phenotype. Source: SGD vacuolar proton-transporting V-type ATPase complex assembly Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	endoplasmic reticulum membrane Inferred from direct assay Ref.7. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuole Inferred from mutant phenotype Ref.7. Source: GOC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	Vacuolar ATPase assembly integral membrane protein VPH2		PRO_0000065874
Regions
	Topological domain	1 – 134	134	Cytoplasmic Potential
	Transmembrane	135 – 155	21	Helical; Potential
	Topological domain	156 – 167	12	Lumenal Potential
	Transmembrane	168 – 186	19	Helical; Potential
	Topological domain	187 – 215	29	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P32341 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 96498A7AC1197C08	FASTA	215	25,287
	10 20 30 40 50 60 MFEIKLNDRI TEFLRKFKNS AKSNEGIDED IDLFLKRHAI PMQSLLFYVK EYRKDSDLQC 70 80 90 100 110 120 SIKELLKPLE FEFKPKAVRG LHYSEDFKKK LEFLKYQEQE LEYQSMVKRS KSVFSLQEDD 130 140 150 160 170 180 ELTPSQINKQ IKEQVTTVFN VLVSVISVVV AIWYWTGSST NFPVHVRLLL CLFFGILVLV 190 200 210 ADVVVYNSYL KKLEEAKVKE KTKVEKKKVL SKITL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae." Hirata R., Umemoto N., Ho M.N., Ohya Y., Stevens T.H., Anraku Y. J. Biol. Chem. 268:961-967(1993) [PubMed: 8419376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The VPH2 gene encodes a 25 kDa protein required for activity of the yeast vacuolar H(+)-ATPase." Bachhawat A.K., Manolson M.F., Murdock D.G., Garman J.D., Jones E.W. Yeast 9:175-184(1993) [PubMed: 8465604] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases." Jacquier A., Legrain P., Dujon B. Yeast 8:121-132(1992) [PubMed: 1561835] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
[6]	"Sequence of a segment of yeast chromosome XI identifies a new mitochondrial carrier, a new member of the G protein family, and a protein with the PAAKK motif of the H1 histones." Colleaux L., Richard G.-F., Thierry A., Dujon B. Yeast 8:325-336(1992) [PubMed: 1514329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-215.
[7]	"VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly." Jackson D.D., Stevens T.H. J. Biol. Chem. 272:25928-25934(1997) [PubMed: 9325326] [Abstract] Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11472 Genomic DNA. Translation: BAA02029.1. M93350 Genomic DNA. Translation: AAA35212.1. Z28118 Genomic DNA. Translation: CAA81960.1. S93804 Genomic DNA. Translation: AAB22004.1. S44213 Genomic DNA. Translation: AAB23070.2. BK006944 Genomic DNA. Translation: DAA09041.1.
PIR	S25358.
RefSeq	NP_012803.1. NM_001179685.1.
3D structure databases
ProteinModelPortal	P32341.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5907N.
IntAct	P32341. 4 interactions.
MINT	MINT-687909.
STRING	P32341.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL119C; YKL119C; YKL119C.
GeneID	853741.
KEGG	sce:YKL119C.
NMPDR	fig|4932.3.peg.3784.
Organism-specific databases
CYGD	YKL119c.
SGD	S000001602. VPH2.
Phylogenomic databases
eggNOG	fuNOG11980.
HOGENOM	HBG202418.
OMA	VVAIWYW.
OrthoDB	EOG4JQ77F.
Gene expression databases
ArrayExpress	P32341.
Genevestigator	P32341.
GermOnline	YKL119C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021013. ATPase_Vma12. [Graphical view]
Pfam	PF11712. Vma12. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974793.

Entry information

Entry name

VPH2_YEAST

Accession

Primary (citable) accession number: P32341
Secondary accession number(s): D6VXH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program