SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P32341

- VPH2_YEAST

UniProt

P32341 - VPH2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Vacuolar ATPase assembly integral membrane protein VPH2

Gene
VPH2, CLS10, VMA12, YKL119C, YKL520
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Required for vacuolar ATPase assembly.

GO - Biological processi

  1. vacuolar acidification Source: SGD
  2. vacuolar proton-transporting V-type ATPase complex assembly Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31902-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar ATPase assembly integral membrane protein VPH2
Alternative name(s):
Protein VMA12
Gene namesi
Name:VPH2
Synonyms:CLS10, VMA12
Ordered Locus Names:YKL119C
ORF Names:YKL520
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL119c.
SGDiS000001602. VPH2.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 134134Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei135 – 15521Helical; Reviewed predictionAdd
BLAST
Topological domaini156 – 16712Lumenal Reviewed predictionAdd
BLAST
Transmembranei168 – 18619Helical; Reviewed predictionAdd
BLAST
Topological domaini187 – 21529Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Vacuolar ATPase assembly integral membrane protein VPH2PRO_0000065874Add
BLAST

Proteomic databases

MaxQBiP32341.
PaxDbiP32341.

Expressioni

Gene expression databases

GenevestigatoriP32341.

Interactioni

Protein-protein interaction databases

BioGridi34016. 219 interactions.
DIPiDIP-5907N.
IntActiP32341. 2 interactions.
MINTiMINT-687909.
STRINGi4932.YKL119C.

Structurei

3D structure databases

ProteinModelPortaliP32341.

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72566.
HOGENOMiHOG000246816.
OMAiMTINENP.
OrthoDBiEOG7PS1SN.

Family and domain databases

InterProiIPR021013. ATPase_Vma12.
[Graphical view]
PfamiPF11712. Vma12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32341-1 [UniParc]FASTAAdd to Basket

« Hide

MFEIKLNDRI TEFLRKFKNS AKSNEGIDED IDLFLKRHAI PMQSLLFYVK    50
EYRKDSDLQC SIKELLKPLE FEFKPKAVRG LHYSEDFKKK LEFLKYQEQE 100
LEYQSMVKRS KSVFSLQEDD ELTPSQINKQ IKEQVTTVFN VLVSVISVVV 150
AIWYWTGSST NFPVHVRLLL CLFFGILVLV ADVVVYNSYL KKLEEAKVKE 200
KTKVEKKKVL SKITL 215
Length:215
Mass (Da):25,287
Last modified:October 1, 1993 - v1
Checksum:i96498A7AC1197C08
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11472 Genomic DNA. Translation: BAA02029.1.
M93350 Genomic DNA. Translation: AAA35212.1.
Z28118 Genomic DNA. Translation: CAA81960.1.
S93804 Genomic DNA. Translation: AAB22004.1.
S44213 Genomic DNA. Translation: AAB23070.2.
BK006944 Genomic DNA. Translation: DAA09041.1.
PIRiS25358.
RefSeqiNP_012803.1. NM_001179685.1.

Genome annotation databases

EnsemblFungiiYKL119C; YKL119C; YKL119C.
GeneIDi853741.
KEGGisce:YKL119C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D11472 Genomic DNA. Translation: BAA02029.1 .
M93350 Genomic DNA. Translation: AAA35212.1 .
Z28118 Genomic DNA. Translation: CAA81960.1 .
S93804 Genomic DNA. Translation: AAB22004.1 .
S44213 Genomic DNA. Translation: AAB23070.2 .
BK006944 Genomic DNA. Translation: DAA09041.1 .
PIRi S25358.
RefSeqi NP_012803.1. NM_001179685.1.

3D structure databases

ProteinModelPortali P32341.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34016. 219 interactions.
DIPi DIP-5907N.
IntActi P32341. 2 interactions.
MINTi MINT-687909.
STRINGi 4932.YKL119C.

Proteomic databases

MaxQBi P32341.
PaxDbi P32341.

Protocols and materials databases

DNASUi 853741.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL119C ; YKL119C ; YKL119C .
GeneIDi 853741.
KEGGi sce:YKL119C.

Organism-specific databases

CYGDi YKL119c.
SGDi S000001602. VPH2.

Phylogenomic databases

eggNOGi NOG72566.
HOGENOMi HOG000246816.
OMAi MTINENP.
OrthoDBi EOG7PS1SN.

Enzyme and pathway databases

BioCyci YEAST:G3O-31902-MONOMER.

Miscellaneous databases

NextBioi 974793.

Gene expression databases

Genevestigatori P32341.

Family and domain databases

InterProi IPR021013. ATPase_Vma12.
[Graphical view ]
Pfami PF11712. Vma12. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae."
    Hirata R., Umemoto N., Ho M.N., Ohya Y., Stevens T.H., Anraku Y.
    J. Biol. Chem. 268:961-967(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The VPH2 gene encodes a 25 kDa protein required for activity of the yeast vacuolar H(+)-ATPase."
    Bachhawat A.K., Manolson M.F., Murdock D.G., Garman J.D., Jones E.W.
    Yeast 9:175-184(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases."
    Jacquier A., Legrain P., Dujon B.
    Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
  6. "Sequence of a segment of yeast chromosome XI identifies a new mitochondrial carrier, a new member of the G protein family, and a protein with the PAAKK motif of the H1 histones."
    Colleaux L., Richard G.-F., Thierry A., Dujon B.
    Yeast 8:325-336(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-215.
  7. "VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly."
    Jackson D.D., Stevens T.H.
    J. Biol. Chem. 272:25928-25934(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVPH2_YEAST
AccessioniPrimary (citable) accession number: P32341
Secondary accession number(s): D6VXH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2030 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi