Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P32341

- VPH2_YEAST

UniProt

P32341 - VPH2_YEAST

Protein

Vacuolar ATPase assembly integral membrane protein VPH2

Gene

VPH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for vacuolar ATPase assembly.

    GO - Biological processi

    1. vacuolar acidification Source: SGD
    2. vacuolar proton-transporting V-type ATPase complex assembly Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31902-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar ATPase assembly integral membrane protein VPH2
    Alternative name(s):
    Protein VMA12
    Gene namesi
    Name:VPH2
    Synonyms:CLS10, VMA12
    Ordered Locus Names:YKL119C
    ORF Names:YKL520
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL119c.
    SGDiS000001602. VPH2.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: SGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 215215Vacuolar ATPase assembly integral membrane protein VPH2PRO_0000065874Add
    BLAST

    Proteomic databases

    MaxQBiP32341.
    PaxDbiP32341.

    Expressioni

    Gene expression databases

    GenevestigatoriP32341.

    Interactioni

    Protein-protein interaction databases

    BioGridi34016. 219 interactions.
    DIPiDIP-5907N.
    IntActiP32341. 2 interactions.
    MINTiMINT-687909.
    STRINGi4932.YKL119C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32341.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 134134CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini156 – 16712LumenalSequence AnalysisAdd
    BLAST
    Topological domaini187 – 21529CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei135 – 15521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei168 – 18619HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG72566.
    HOGENOMiHOG000246816.
    OMAiMTINENP.
    OrthoDBiEOG7PS1SN.

    Family and domain databases

    InterProiIPR021013. ATPase_Vma12.
    [Graphical view]
    PfamiPF11712. Vma12. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32341-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFEIKLNDRI TEFLRKFKNS AKSNEGIDED IDLFLKRHAI PMQSLLFYVK    50
    EYRKDSDLQC SIKELLKPLE FEFKPKAVRG LHYSEDFKKK LEFLKYQEQE 100
    LEYQSMVKRS KSVFSLQEDD ELTPSQINKQ IKEQVTTVFN VLVSVISVVV 150
    AIWYWTGSST NFPVHVRLLL CLFFGILVLV ADVVVYNSYL KKLEEAKVKE 200
    KTKVEKKKVL SKITL 215
    Length:215
    Mass (Da):25,287
    Last modified:October 1, 1993 - v1
    Checksum:i96498A7AC1197C08
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11472 Genomic DNA. Translation: BAA02029.1.
    M93350 Genomic DNA. Translation: AAA35212.1.
    Z28118 Genomic DNA. Translation: CAA81960.1.
    S93804 Genomic DNA. Translation: AAB22004.1.
    S44213 Genomic DNA. Translation: AAB23070.2.
    BK006944 Genomic DNA. Translation: DAA09041.1.
    PIRiS25358.
    RefSeqiNP_012803.1. NM_001179685.1.

    Genome annotation databases

    EnsemblFungiiYKL119C; YKL119C; YKL119C.
    GeneIDi853741.
    KEGGisce:YKL119C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11472 Genomic DNA. Translation: BAA02029.1 .
    M93350 Genomic DNA. Translation: AAA35212.1 .
    Z28118 Genomic DNA. Translation: CAA81960.1 .
    S93804 Genomic DNA. Translation: AAB22004.1 .
    S44213 Genomic DNA. Translation: AAB23070.2 .
    BK006944 Genomic DNA. Translation: DAA09041.1 .
    PIRi S25358.
    RefSeqi NP_012803.1. NM_001179685.1.

    3D structure databases

    ProteinModelPortali P32341.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34016. 219 interactions.
    DIPi DIP-5907N.
    IntActi P32341. 2 interactions.
    MINTi MINT-687909.
    STRINGi 4932.YKL119C.

    Proteomic databases

    MaxQBi P32341.
    PaxDbi P32341.

    Protocols and materials databases

    DNASUi 853741.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL119C ; YKL119C ; YKL119C .
    GeneIDi 853741.
    KEGGi sce:YKL119C.

    Organism-specific databases

    CYGDi YKL119c.
    SGDi S000001602. VPH2.

    Phylogenomic databases

    eggNOGi NOG72566.
    HOGENOMi HOG000246816.
    OMAi MTINENP.
    OrthoDBi EOG7PS1SN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31902-MONOMER.

    Miscellaneous databases

    NextBioi 974793.

    Gene expression databases

    Genevestigatori P32341.

    Family and domain databases

    InterProi IPR021013. ATPase_Vma12.
    [Graphical view ]
    Pfami PF11712. Vma12. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae."
      Hirata R., Umemoto N., Ho M.N., Ohya Y., Stevens T.H., Anraku Y.
      J. Biol. Chem. 268:961-967(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The VPH2 gene encodes a 25 kDa protein required for activity of the yeast vacuolar H(+)-ATPase."
      Bachhawat A.K., Manolson M.F., Murdock D.G., Garman J.D., Jones E.W.
      Yeast 9:175-184(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases."
      Jacquier A., Legrain P., Dujon B.
      Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
    6. "Sequence of a segment of yeast chromosome XI identifies a new mitochondrial carrier, a new member of the G protein family, and a protein with the PAAKK motif of the H1 histones."
      Colleaux L., Richard G.-F., Thierry A., Dujon B.
      Yeast 8:325-336(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-215.
    7. "VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly."
      Jackson D.D., Stevens T.H.
      J. Biol. Chem. 272:25928-25934(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVPH2_YEAST
    AccessioniPrimary (citable) accession number: P32341
    Secondary accession number(s): D6VXH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2030 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3