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P32341 (VPH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar ATPase assembly integral membrane protein VPH2
Alternative name(s):
Protein VMA12
Gene names
Name:VPH2
Synonyms:CLS10, VMA12
Ordered Locus Names:YKL119C
ORF Names:YKL520
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length215 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for vacuolar ATPase assembly.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.7.

Miscellaneous

Present with 2030 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 215215Vacuolar ATPase assembly integral membrane protein VPH2
PRO_0000065874

Regions

Topological domain1 – 134134Cytoplasmic Potential
Transmembrane135 – 15521Helical; Potential
Topological domain156 – 16712Lumenal Potential
Transmembrane168 – 18619Helical; Potential
Topological domain187 – 21529Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P32341 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 96498A7AC1197C08

FASTA21525,287
        10         20         30         40         50         60 
MFEIKLNDRI TEFLRKFKNS AKSNEGIDED IDLFLKRHAI PMQSLLFYVK EYRKDSDLQC 

        70         80         90        100        110        120 
SIKELLKPLE FEFKPKAVRG LHYSEDFKKK LEFLKYQEQE LEYQSMVKRS KSVFSLQEDD 

       130        140        150        160        170        180 
ELTPSQINKQ IKEQVTTVFN VLVSVISVVV AIWYWTGSST NFPVHVRLLL CLFFGILVLV 

       190        200        210 
ADVVVYNSYL KKLEEAKVKE KTKVEKKKVL SKITL 

« Hide

References

« Hide 'large scale' references
[1]"VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto the vacuolar membrane in Saccharomyces cerevisiae."
Hirata R., Umemoto N., Ho M.N., Ohya Y., Stevens T.H., Anraku Y.
J. Biol. Chem. 268:961-967(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The VPH2 gene encodes a 25 kDa protein required for activity of the yeast vacuolar H(+)-ATPase."
Bachhawat A.K., Manolson M.F., Murdock D.G., Garman J.D., Jones E.W.
Yeast 9:175-184(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases."
Jacquier A., Legrain P., Dujon B.
Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
[6]"Sequence of a segment of yeast chromosome XI identifies a new mitochondrial carrier, a new member of the G protein family, and a protein with the PAAKK motif of the H1 histones."
Colleaux L., Richard G.-F., Thierry A., Dujon B.
Yeast 8:325-336(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-215.
[7]"VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly."
Jackson D.D., Stevens T.H.
J. Biol. Chem. 272:25928-25934(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D11472 Genomic DNA. Translation: BAA02029.1.
M93350 Genomic DNA. Translation: AAA35212.1.
Z28118 Genomic DNA. Translation: CAA81960.1.
S93804 Genomic DNA. Translation: AAB22004.1.
S44213 Genomic DNA. Translation: AAB23070.2.
BK006944 Genomic DNA. Translation: DAA09041.1.
PIRS25358.
RefSeqNP_012803.1. NM_001179685.1.

3D structure databases

ProteinModelPortalP32341.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34016. 219 interactions.
DIPDIP-5907N.
IntActP32341. 2 interactions.
MINTMINT-687909.
STRING4932.YKL119C.

Proteomic databases

MaxQBP32341.
PaxDbP32341.

Protocols and materials databases

DNASU853741.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL119C; YKL119C; YKL119C.
GeneID853741.
KEGGsce:YKL119C.

Organism-specific databases

CYGDYKL119c.
SGDS000001602. VPH2.

Phylogenomic databases

eggNOGNOG72566.
HOGENOMHOG000246816.
OMAMTINENP.
OrthoDBEOG7PS1SN.

Enzyme and pathway databases

BioCycYEAST:G3O-31902-MONOMER.

Gene expression databases

GenevestigatorP32341.

Family and domain databases

InterProIPR021013. ATPase_Vma12.
[Graphical view]
PfamPF11712. Vma12. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974793.

Entry information

Entry nameVPH2_YEAST
AccessionPrimary (citable) accession number: P32341
Secondary accession number(s): D6VXH1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD