Reviewed, UniProtKB/Swiss-Prot P32340 (NDI1_YEAST)

Names and origin

Protein names

Recommended name:
    Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial
    EC=1.6.5.3
Alternative name(s):
    Internal NADH dehydrogenase

Gene names

Name:	NDI1
Ordered Locus Names:	YML120C
ORF Names:	YM7056.06C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of NADH generated inside the Mitochondrion.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Note: Bound to the mitochondrial inner membrane facing the matrix site. Ref.4
Miscellaneous	Present with 5240 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the NADH dehydrogenase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	NADH oxidation Inferred from direct assay. Source: SGD chronological cell aging Inferred from mutant phenotype. Source: SGD mitochondrial electron transport, NADH to ubiquinone Inferred from direct assay. Source: SGD positive regulation of apoptosis Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial matrix Inferred from direct assay. Source: SGD
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERG11	P10614	1	EBI-11961,EBI-5127

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Mitochondrion Ref.1
	Chain	27 – 513	487	Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial		PRO_0000021793
Regions
	Nucleotide binding	55 – 85	31	FAD By similarity
	Nucleotide binding	229 – 265	37	NAD By similarity
Amino acid modifications
	Modified residue	27	1	Phosphoserine Ref.6
	Modified residue	28	1	Phosphothreonine Ref.6
Natural variations
	Natural variant	10	1	K → R

Sequences

	Sequence		Length	Mass (Da)
	P32340-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: E3A43D75A1ADCF3B	FASTA	513	57,250
	10 20 30 40 50 60 MLSKNLYSNK RLLTSTNTLV RFASTRSTGV ENSGAGPTSF KTMKVIDPQH SDKPNVLILG 70 80 90 100 110 120 SGWGAISFLK HIDTKKYNVS IISPRSYFLF TPLLPSAPVG TVDEKSIIEP IVNFALKKKG 130 140 150 160 170 180 NVTYYEAEAT SINPDRNTVT IKSLSAVSQL YQPENHLGLH QAEPAEIKYD YLISAVGAEP 190 200 210 220 230 240 NTFGIPGVTD YGHFLKEIPN SLEIRRTFAA NLEKANLLPK GDPERRRLLS IVVVGGGPTG 250 260 270 280 290 300 VEAAGELQDY VHQDLRKFLP ALAEEVQIHL VEALPIVLNM FEKKLSSYAQ SHLENTSIKV 310 320 330 340 350 360 HLRTAVAKVE EKQLLAKTKH EDGKITEETI PYGTLIWATG NKARPVITDL FKKIPEQNSS 370 380 390 400 410 420 KRGLAVNDFL QVKGSNNIFA IGDNAFAGLP PTAQVAHQEA EYLAKNFDKM AQIPNFQKNL 430 440 450 460 470 480 SSRKDKIDLL FEENNFKPFK YNDLGALAYL GSERAIATIR SGKRTFYTGG GLMTFYLWRI 490 500 510 LYLSMILSAR SRLKVFFDWI KLAFFKRDFF KGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure and import pathway of the rotenone-insensitive NADH-ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae." de Vries S., van Witzenburg R., Grivell L.A., Marres C.A.M. Eur. J. Biochem. 203:587-592(1992) [PubMed: 1735444] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-36 AND 41-50. Strain: YP102.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed: 11502169] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27 AND THR-28, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X61590 Genomic DNA. Translation: CAA43787.1. Sequence problems. Z49218 Genomic DNA. Translation: CAA89160.1. AY723851 Genomic DNA. Translation: AAU09768.1.
PIR	S26704.
RefSeq	NP_013586.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5554N.
IntAct	P32340. 58 interactions.
Proteomic databases
PeptideAtlas	P32340.
Genome annotation databases
Ensembl	YML120C. Saccharomyces cerevisiae. [Contig view]
GeneID	854919.
GenomeReviews	Gene locus YML120C in contig Z71257_GR.
KEGG	sce:YML120C.
NMPDR	fig|4932.3.peg.4622.
Organism-specific databases
CYGD	YML120c.
SGD	S000004589. NDI1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P32340.
OMA	P32340. NTVTIKS.
Enzyme and pathway databases
BRENDA	1.6.5.3. 250.
Gene expression databases
ArrayExpress	P32340.
GermOnline	YML120C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977929.

Entry information

Entry name

NDI1_YEAST

Accession

Primary (citable) accession number: P32340

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)