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P32340 (NDI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial

EC=1.6.5.9
Alternative name(s):
Internal NADH dehydrogenase
NADH:ubiquinone reductase (non-electrogenic)
Gene names
Name:NDI1
Ordered Locus Names:YML120C
ORF Names:YM7056.06C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidation of NADH generated inside the Mitochondrion.

Catalytic activity

NADH + ubiquinone = NAD+ + ubiquinol.

Cofactor

Binds 1 FAD per subunit By similarity.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Note: Bound to the mitochondrial inner membrane facing the matrix site. Ref.5

Miscellaneous

Present with 5240 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the NADH dehydrogenase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ERG11P106141EBI-11961,EBI-5127

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2626Mitochondrion Ref.1
Chain27 – 513487Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial
PRO_0000021793

Regions

Nucleotide binding55 – 8531FAD By similarity
Nucleotide binding229 – 26537NAD By similarity

Natural variations

Natural variant101K → R.

Secondary structure

.................................................................................................. 513
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P32340 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: E3A43D75A1ADCF3B

FASTA51357,250
        10         20         30         40         50         60 
MLSKNLYSNK RLLTSTNTLV RFASTRSTGV ENSGAGPTSF KTMKVIDPQH SDKPNVLILG 

        70         80         90        100        110        120 
SGWGAISFLK HIDTKKYNVS IISPRSYFLF TPLLPSAPVG TVDEKSIIEP IVNFALKKKG 

       130        140        150        160        170        180 
NVTYYEAEAT SINPDRNTVT IKSLSAVSQL YQPENHLGLH QAEPAEIKYD YLISAVGAEP 

       190        200        210        220        230        240 
NTFGIPGVTD YGHFLKEIPN SLEIRRTFAA NLEKANLLPK GDPERRRLLS IVVVGGGPTG 

       250        260        270        280        290        300 
VEAAGELQDY VHQDLRKFLP ALAEEVQIHL VEALPIVLNM FEKKLSSYAQ SHLENTSIKV 

       310        320        330        340        350        360 
HLRTAVAKVE EKQLLAKTKH EDGKITEETI PYGTLIWATG NKARPVITDL FKKIPEQNSS 

       370        380        390        400        410        420 
KRGLAVNDFL QVKGSNNIFA IGDNAFAGLP PTAQVAHQEA EYLAKNFDKM AQIPNFQKNL 

       430        440        450        460        470        480 
SSRKDKIDLL FEENNFKPFK YNDLGALAYL GSERAIATIR SGKRTFYTGG GLMTFYLWRI 

       490        500        510 
LYLSMILSAR SRLKVFFDWI KLAFFKRDFF KGL 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure and import pathway of the rotenone-insensitive NADH-ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae."
de Vries S., van Witzenburg R., Grivell L.A., Marres C.A.M.
Eur. J. Biochem. 203:587-592(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-36 AND 41-50.
Strain: YP102.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X61590 Genomic DNA. Translation: CAA43787.1. Sequence problems.
Z49218 Genomic DNA. Translation: CAA89160.1.
AY723851 Genomic DNA. Translation: AAU09768.1.
BK006946 Genomic DNA. Translation: DAA09778.1.
PIRS26704.
RefSeqNP_013586.1. NM_001182483.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4G6GX-ray2.39A/B24-513[»]
4G6HX-ray2.26A/B24-513[»]
4G73X-ray2.52A/B24-513[»]
4G74X-ray2.48A/B24-513[»]
4G9KX-ray2.70A/B43-513[»]
4GAPX-ray2.90A/B43-513[»]
4GAVX-ray3.00A/B43-513[»]
ProteinModelPortalP32340.
SMRP32340. Positions 42-513.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35084. 34 interactions.
DIPDIP-5554N.
IntActP32340. 15 interactions.
MINTMINT-556740.
STRING4932.YML120C.

2D gel databases

UCD-2DPAGEP32340.

Proteomic databases

MaxQBP32340.
PaxDbP32340.
PeptideAtlasP32340.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML120C; YML120C; YML120C.
GeneID854919.
KEGGsce:YML120C.

Organism-specific databases

CYGDYML120c.
SGDS000004589. NDI1.

Phylogenomic databases

eggNOGCOG1252.
GeneTreeENSGT00530000065152.
HOGENOMHOG000182501.
KOK17871.
OMAPMLYQVA.
OrthoDBEOG7034S6.

Enzyme and pathway databases

BioCycMetaCyc:YML120C-MONOMER.
YEAST:YML120C-MONOMER.

Gene expression databases

GenevestigatorP32340.

Family and domain databases

InterProIPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 2 hits.
[Graphical view]
PRINTSPR00368. FADPNR.
ProtoNetSearch...

Other

NextBio977929.
PROP32340.

Entry information

Entry nameNDI1_YEAST
AccessionPrimary (citable) accession number: P32340
Secondary accession number(s): D6W0G4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references