Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P32340 (NDI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 122. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial
EC=1.6.5.9

Alternative name(s):
Internal NADH dehydrogenase
NADH:ubiquinone reductase (non-electrogenic)

Gene names

Name:	NDI1
Ordered Locus Names:	YML120C
ORF Names:	YM7056.06C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidation of NADH generated inside the Mitochondrion.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Note: Bound to the mitochondrial inner membrane facing the matrix site. Ref.5
Miscellaneous	Present with 5240 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the NADH dehydrogenase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	NADH oxidation Inferred from direct assay. Source: SGD chronological cell aging Inferred from mutant phenotype. Source: SGD mitochondrial electron transport, NADH to ubiquinone Inferred from direct assay. Source: SGD positive regulation of apoptotic process Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial matrix Inferred from direct assay. Source: SGD
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from direct assay. Source: SGD flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERG11	P10614	1	EBI-11961,EBI-5127

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 26

Mitochondrion Ref.1

Chain

27 – 513

487

Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial

PRO_0000021793

Regions

Nucleotide binding

55 – 85

FAD By similarity

Nucleotide binding

229 – 265

NAD By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Modified residue

Phosphothreonine Ref.7

Natural variations

Natural variant

K → R.

Sequences

Sequence

Length

Mass (Da)

Tools

P32340 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: E3A43D75A1ADCF3B
Show »

FASTA

513

57,250

        10         20         30         40         50         60 
MLSKNLYSNK RLLTSTNTLV RFASTRSTGV ENSGAGPTSF KTMKVIDPQH SDKPNVLILG 

        70         80         90        100        110        120 
SGWGAISFLK HIDTKKYNVS IISPRSYFLF TPLLPSAPVG TVDEKSIIEP IVNFALKKKG 

       130        140        150        160        170        180 
NVTYYEAEAT SINPDRNTVT IKSLSAVSQL YQPENHLGLH QAEPAEIKYD YLISAVGAEP 

       190        200        210        220        230        240 
NTFGIPGVTD YGHFLKEIPN SLEIRRTFAA NLEKANLLPK GDPERRRLLS IVVVGGGPTG 

       250        260        270        280        290        300 
VEAAGELQDY VHQDLRKFLP ALAEEVQIHL VEALPIVLNM FEKKLSSYAQ SHLENTSIKV 

       310        320        330        340        350        360 
HLRTAVAKVE EKQLLAKTKH EDGKITEETI PYGTLIWATG NKARPVITDL FKKIPEQNSS 

       370        380        390        400        410        420 
KRGLAVNDFL QVKGSNNIFA IGDNAFAGLP PTAQVAHQEA EYLAKNFDKM AQIPNFQKNL 

       430        440        450        460        470        480 
SSRKDKIDLL FEENNFKPFK YNDLGALAYL GSERAIATIR SGKRTFYTGG GLMTFYLWRI 

       490        500        510 
LYLSMILSAR SRLKVFFDWI KLAFFKRDFF KGL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure and import pathway of the rotenone-insensitive NADH-ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae." de Vries S., van Witzenburg R., Grivell L.A., Marres C.A.M. Eur. J. Biochem. 203:587-592(1992) [PubMed: 1735444] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-36 AND 41-50. Strain: YP102.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed: 11502169] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27 AND THR-28, MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61590 Genomic DNA. Translation: CAA43787.1. Sequence problems. Z49218 Genomic DNA. Translation: CAA89160.1. AY723851 Genomic DNA. Translation: AAU09768.1. BK006946 Genomic DNA. Translation: DAA09778.1.
PIR	S26704.
RefSeq	NP_013586.1. NM_001182483.1.
3D structure databases
ProteinModelPortal	P32340.
SMR	P32340. Positions 52-86, 230-345, 375-408.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5554N.
IntAct	P32340. 15 interactions.
MINT	MINT-556740.
STRING	P32340.
2D gel databases
UCD-2DPAGE	P32340.
Proteomic databases
PeptideAtlas	P32340.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML120C; YML120C; YML120C.
GeneID	854919.
KEGG	sce:YML120C.
NMPDR	fig\|4932.3.peg.4622.
Organism-specific databases
CYGD	YML120c.
SGD	S000004589. NDI1.
Phylogenomic databases
eggNOG	fuNOG08220.
GeneTree	EFGT00050000003551.
HOGENOM	HBG319217.
OMA	DRNTVTI.
OrthoDB	EOG4TQQJG.
Gene expression databases
ArrayExpress	P32340.
Genevestigator	P32340.
GermOnline	YML120C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. [Graphical view]
KO	K03885.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 2 hits. [Graphical view]
PRINTS	PR00368. FADPNR.
ProtoNet	Search...
Other
NextBio	977929.

Entry information

Entry name

NDI1_YEAST

Accession

Primary (citable) accession number: P32340
Secondary accession number(s): D6W0G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents