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P32337

- IMB3_YEAST

UniProt

P32337 - IMB3_YEAST

Protein

Importin subunit beta-3

Gene

PSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in the nuclear import of ribosomal proteins. Binds to nucleoporins and the GTP-bound form of GSP1 (Ran).
    Plays a role in protein secretion.

    GO - Molecular functioni

    1. nuclear localization sequence binding Source: SGD
    2. protein transporter activity Source: SGD

    GO - Biological processi

    1. mRNA export from nucleus Source: SGD
    2. protein import into nucleus Source: SGD
    3. regulation of mitosis Source: SGD
    4. regulation of protein desumoylation Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32972-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Importin subunit beta-3
    Alternative name(s):
    Karyopherin subunit beta-3
    Karyopherin-121
    Protein secretion enhancer 1
    Gene namesi
    Name:PSE1
    Synonyms:KAP121
    Ordered Locus Names:YMR308C
    ORF Names:YM9952.10C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR308c.
    SGDiS000004925. PSE1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 10891088Importin subunit beta-3PRO_0000120774Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei830 – 8301Phosphothreonine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP32337.
    PaxDbiP32337.
    PeptideAtlasiP32337.

    Expressioni

    Gene expression databases

    GenevestigatoriP32337.

    Interactioni

    Subunit structurei

    Interacts with ABF1.1 Publication

    Protein-protein interaction databases

    BioGridi35488. 152 interactions.
    DIPiDIP-1533N.
    IntActiP32337. 73 interactions.
    MINTiMINT-390897.
    STRINGi4932.YMR308C.

    Structurei

    Secondary structure

    1
    1089
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 1813
    Helixi25 – 3713
    Turni41 – 433
    Helixi44 – 5714
    Helixi61 – 7717
    Helixi91 – 933
    Helixi96 – 11116
    Helixi116 – 12712
    Helixi138 – 14912
    Helixi153 – 16513
    Helixi168 – 1725
    Helixi175 – 18511
    Helixi191 – 20717
    Helixi210 – 2156
    Helixi217 – 2193
    Helixi220 – 2267
    Helixi228 – 2325
    Helixi236 – 25217
    Helixi254 – 2607
    Helixi261 – 27313
    Helixi279 – 29517
    Helixi297 – 3015
    Helixi304 – 31815
    Helixi329 – 3324
    Helixi343 – 35917
    Helixi361 – 37616
    Helixi381 – 39414
    Turni395 – 3984
    Helixi399 – 4024
    Helixi406 – 4138
    Helixi414 – 4185
    Helixi422 – 43817
    Turni439 – 4413
    Helixi442 – 45817
    Helixi465 – 48016
    Helixi484 – 4874
    Helixi488 – 4903
    Helixi491 – 50212
    Helixi507 – 52418
    Helixi525 – 5317
    Helixi532 – 54413
    Helixi551 – 56818
    Helixi570 – 5734
    Turni574 – 5763
    Helixi577 – 58812
    Helixi597 – 61317
    Helixi614 – 6207
    Helixi621 – 63212
    Beta strandi638 – 6414
    Beta strandi646 – 6483
    Helixi649 – 6513
    Beta strandi655 – 66814
    Helixi669 – 68921
    Helixi690 – 6934
    Helixi694 – 70310
    Helixi705 – 7095
    Helixi715 – 73420
    Helixi742 – 75716
    Turni758 – 7603
    Helixi764 – 78118
    Helixi788 – 80922
    Helixi829 – 84921
    Turni850 – 8534
    Helixi854 – 8574
    Helixi858 – 8603
    Helixi861 – 8688
    Helixi873 – 88715
    Helixi891 – 8933
    Helixi895 – 90915
    Helixi914 – 93017
    Turni933 – 9353
    Helixi936 – 94914
    Helixi958 – 9603
    Helixi961 – 97717
    Turni978 – 9803
    Helixi987 – 9937
    Helixi1002 – 101312
    Helixi1029 – 104113
    Helixi1053 – 106210
    Helixi1066 – 10716
    Helixi1072 – 10754
    Helixi1078 – 10858

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3W3TX-ray2.90A1-1089[»]
    3W3UX-ray2.60A1-1089[»]
    3W3VX-ray3.20A1-1089[»]
    3W3WX-ray2.20A1-1089[»]
    3W3XX-ray2.90A1-1089[»]
    3W3YX-ray2.80A1-1089[»]
    3W3ZX-ray2.70A1-1089[»]
    ProteinModelPortaliP32337.
    SMRiP32337. Positions 133-549.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati6 – 4338HEAT 1Add
    BLAST
    Repeati135 – 17238HEAT 2Add
    BLAST
    Repeati174 – 21037HEAT 3Add
    BLAST
    Repeati404 – 44138HEAT 4Add
    BLAST
    Repeati446 – 48439HEAT 5Add
    BLAST
    Repeati489 – 52638HEAT 6Add
    BLAST
    Repeati530 – 57041HEAT 7Add
    BLAST
    Repeati719 – 75638HEAT 8Add
    BLAST
    Repeati896 – 93338HEAT 9Add
    BLAST

    Sequence similaritiesi

    Belongs to the importin beta family.Curated
    Contains 9 HEAT repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG249123.
    GeneTreeiENSGT00550000074773.
    HOGENOMiHOG000209725.
    OMAiNCNLPRI.
    OrthoDBiEOG7WX0HQ.

    Family and domain databases

    Gene3Di1.25.10.10. 2 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32337-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSALPEEVNR TLLQIVQAFA SPDNQIRSVA EKALSEEWIT ENNIEYLLTF     50
    LAEQAAFSQD TTVAALSAVL FRKLALKAPP SSKLMIMSKN ITHIRKEVLA 100
    QIRSSLLKGF LSERADSIRH KLSDAIAECV QDDLPAWPEL LQALIESLKS 150
    GNPNFRESSF RILTTVPYLI TAVDINSILP IFQSGFTDAS DNVKIAAVTA 200
    FVGYFKQLPK SEWSKLGILL PSLLNSLPRF LDDGKDDALA SVFESLIELV 250
    ELAPKLFKDM FDQIIQFTDM VIKNKDLEPP ARTTALELLT VFSENAPQMC 300
    KSNQNYGQTL VMVTLIMMTE VSIDDDDAAE WIESDDTDDE EEVTYDHARQ 350
    ALDRVALKLG GEYLAAPLFQ YLQQMITSTE WRERFAAMMA LSSAAEGCAD 400
    VLIGEIPKIL DMVIPLINDP HPRVQYGCCN VLGQISTDFS PFIQRTAHDR 450
    ILPALISKLT SECTSRVQTH AAAALVNFSE FASKDILEPY LDSLLTNLLV 500
    LLQSNKLYVQ EQALTTIAFI AEAAKNKFIK YYDTLMPLLL NVLKVNNKDN 550
    SVLKGKCMEC ATLIGFAVGK EKFHEHSQEL ISILVALQNS DIDEDDALRS 600
    YLEQSWSRIC RILGDDFVPL LPIVIPPLLI TAKATQDVGL IEEEEAANFQ 650
    QYPDWDVVQV QGKHIAIHTS VLDDKVSAME LLQSYATLLR GQFAVYVKEV 700
    MEEIALPSLD FYLHDGVRAA GATLIPILLS CLLAATGTQN EELVLLWHKA 750
    SSKLIGGLMS EPMPEITQVY HNSLVNGIKV MGDNCLSEDQ LAAFTKGVSA 800
    NLTDTYERMQ DRHGDGDEYN ENIDEEEDFT DEDLLDEINK SIAAVLKTTN 850
    GHYLKNLENI WPMINTFLLD NEPILVIFAL VVIGDLIQYG GEQTASMKNA 900
    FIPKVTECLI SPDARIRQAA SYIIGVCAQY APSTYADVCI PTLDTLVQIV 950
    DFPGSKLEEN RSSTENASAA IAKILYAYNS NIPNVDTYTA NWFKTLPTIT 1000
    DKEAASFNYQ FLSQLIENNS PIVCAQSNIS AVVDSVIQAL NERSLTEREG 1050
    QTVISSVKKL LGFLPSSDAM AIFNRYPADI MEKVHKWFA 1089
    Length:1,089
    Mass (Da):121,031
    Last modified:October 1, 1996 - v2
    Checksum:i9C5F7FB5B8824C37
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651A → S in CAA77639. (PubMed:1522152)Curated
    Sequence conflicti65 – 651A → S in AAA10665. (PubMed:1522152)Curated
    Sequence conflicti1077 – 10771P → A in CAA77639. (PubMed:1522152)Curated
    Sequence conflicti1077 – 10771P → A in AAA10665. (PubMed:1522152)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11538 Genomic DNA. Translation: CAA77639.1.
    S45357 Genomic DNA. Translation: AAA10665.1.
    Z49212 Genomic DNA. Translation: CAA89141.1.
    BK006946 Genomic DNA. Translation: DAA10209.1.
    PIRiS53978.
    RefSeqiNP_014039.1. NM_001182819.1.

    Genome annotation databases

    EnsemblFungiiYMR308C; YMR308C; YMR308C.
    GeneIDi855356.
    KEGGisce:YMR308C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11538 Genomic DNA. Translation: CAA77639.1 .
    S45357 Genomic DNA. Translation: AAA10665.1 .
    Z49212 Genomic DNA. Translation: CAA89141.1 .
    BK006946 Genomic DNA. Translation: DAA10209.1 .
    PIRi S53978.
    RefSeqi NP_014039.1. NM_001182819.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3W3T X-ray 2.90 A 1-1089 [» ]
    3W3U X-ray 2.60 A 1-1089 [» ]
    3W3V X-ray 3.20 A 1-1089 [» ]
    3W3W X-ray 2.20 A 1-1089 [» ]
    3W3X X-ray 2.90 A 1-1089 [» ]
    3W3Y X-ray 2.80 A 1-1089 [» ]
    3W3Z X-ray 2.70 A 1-1089 [» ]
    ProteinModelPortali P32337.
    SMRi P32337. Positions 133-549.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35488. 152 interactions.
    DIPi DIP-1533N.
    IntActi P32337. 73 interactions.
    MINTi MINT-390897.
    STRINGi 4932.YMR308C.

    Proteomic databases

    MaxQBi P32337.
    PaxDbi P32337.
    PeptideAtlasi P32337.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR308C ; YMR308C ; YMR308C .
    GeneIDi 855356.
    KEGGi sce:YMR308C.

    Organism-specific databases

    CYGDi YMR308c.
    SGDi S000004925. PSE1.

    Phylogenomic databases

    eggNOGi NOG249123.
    GeneTreei ENSGT00550000074773.
    HOGENOMi HOG000209725.
    OMAi NCNLPRI.
    OrthoDBi EOG7WX0HQ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32972-MONOMER.

    Miscellaneous databases

    NextBioi 979118.

    Gene expression databases

    Genevestigatori P32337.

    Family and domain databases

    Gene3Di 1.25.10.10. 2 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Screening and identification of a gene, PSE-1, that affects protein secretion in Saccharomyces cerevisiae."
      Chow T.Y.-K., Ash J.J., Dignard D., Thomas D.Y.
      J. Cell Sci. 101:709-719(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Functional and physical interactions between autonomously replicating sequence-binding factor 1 and the nuclear transport machinery."
      Loch C.M., Mosammaparast N., Miyake T., Pemberton L.F., Li R.
      Traffic 5:925-935(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ABF1.
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-830, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiIMB3_YEAST
    AccessioniPrimary (citable) accession number: P32337
    Secondary accession number(s): D6W0D5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 15500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3