Importin subunit beta-3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Importin subunit beta-3

Alternative name(s):
Karyopherin subunit beta-3
Karyopherin-121
Protein secretion enhancer 1

Gene names

Name:	PSE1
Synonyms:	KAP121
Ordered Locus Names:	YMR308C
ORF Names:	YM9952.10C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1089 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the nuclear import of ribosomal proteins. Binds to nucleoporins and the GTP-bound form of GSP1 (Ran). Plays a role in protein secretion.
Subunit structure	Interacts with ABF1. Ref.5
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Miscellaneous	Present with 15500 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the importin beta family. Contains 9 HEAT repeats.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA export from nucleus Inferred from genetic interaction. Source: SGD protein import into nucleus Inferred from mutant phenotype. Source: SGD regulation of mitosis Inferred from mutant phenotype. Source: SGD regulation of protein desumoylation Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	binding Inferred from electronic annotation. Source: InterPro protein transporter activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1089

1089

Importin subunit beta-3

PRO_0000120774

Regions

Repeat

6 – 43

38

HEAT 1

Repeat

135 – 172

38

HEAT 2

Repeat

174 – 210

37

HEAT 3

Repeat

404 – 441

38

HEAT 4

Repeat

446 – 484

39

HEAT 5

Repeat

489 – 526

38

HEAT 6

Repeat

530 – 570

41

HEAT 7

Repeat

719 – 756

38

HEAT 8

Repeat

896 – 933

38

HEAT 9

Experimental info

Sequence conflict

65

1

A → S in CAA77639. Ref.1

Sequence conflict

65

1

A → S in AAA10665. Ref.1

Sequence conflict

1077

1

P → A in CAA77639. Ref.1

Sequence conflict

1077

1

P → A in AAA10665. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P32337 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 9C5F7FB5B8824C37
Show »

FASTA

1,089

121,031

        10         20         30         40         50         60 
MSALPEEVNR TLLQIVQAFA SPDNQIRSVA EKALSEEWIT ENNIEYLLTF LAEQAAFSQD 

        70         80         90        100        110        120 
TTVAALSAVL FRKLALKAPP SSKLMIMSKN ITHIRKEVLA QIRSSLLKGF LSERADSIRH 

       130        140        150        160        170        180 
KLSDAIAECV QDDLPAWPEL LQALIESLKS GNPNFRESSF RILTTVPYLI TAVDINSILP 

       190        200        210        220        230        240 
IFQSGFTDAS DNVKIAAVTA FVGYFKQLPK SEWSKLGILL PSLLNSLPRF LDDGKDDALA 

       250        260        270        280        290        300 
SVFESLIELV ELAPKLFKDM FDQIIQFTDM VIKNKDLEPP ARTTALELLT VFSENAPQMC 

       310        320        330        340        350        360 
KSNQNYGQTL VMVTLIMMTE VSIDDDDAAE WIESDDTDDE EEVTYDHARQ ALDRVALKLG 

       370        380        390        400        410        420 
GEYLAAPLFQ YLQQMITSTE WRERFAAMMA LSSAAEGCAD VLIGEIPKIL DMVIPLINDP 

       430        440        450        460        470        480 
HPRVQYGCCN VLGQISTDFS PFIQRTAHDR ILPALISKLT SECTSRVQTH AAAALVNFSE 

       490        500        510        520        530        540 
FASKDILEPY LDSLLTNLLV LLQSNKLYVQ EQALTTIAFI AEAAKNKFIK YYDTLMPLLL 

       550        560        570        580        590        600 
NVLKVNNKDN SVLKGKCMEC ATLIGFAVGK EKFHEHSQEL ISILVALQNS DIDEDDALRS 

       610        620        630        640        650        660 
YLEQSWSRIC RILGDDFVPL LPIVIPPLLI TAKATQDVGL IEEEEAANFQ QYPDWDVVQV 

       670        680        690        700        710        720 
QGKHIAIHTS VLDDKVSAME LLQSYATLLR GQFAVYVKEV MEEIALPSLD FYLHDGVRAA 

       730        740        750        760        770        780 
GATLIPILLS CLLAATGTQN EELVLLWHKA SSKLIGGLMS EPMPEITQVY HNSLVNGIKV 

       790        800        810        820        830        840 
MGDNCLSEDQ LAAFTKGVSA NLTDTYERMQ DRHGDGDEYN ENIDEEEDFT DEDLLDEINK 

       850        860        870        880        890        900 
SIAAVLKTTN GHYLKNLENI WPMINTFLLD NEPILVIFAL VVIGDLIQYG GEQTASMKNA 

       910        920        930        940        950        960 
FIPKVTECLI SPDARIRQAA SYIIGVCAQY APSTYADVCI PTLDTLVQIV DFPGSKLEEN 

       970        980        990       1000       1010       1020 
RSSTENASAA IAKILYAYNS NIPNVDTYTA NWFKTLPTIT DKEAASFNYQ FLSQLIENNS 

      1030       1040       1050       1060       1070       1080 
PIVCAQSNIS AVVDSVIQAL NERSLTEREG QTVISSVKKL LGFLPSSDAM AIFNRYPADI 


MEKVHKWFA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Screening and identification of a gene, PSE-1, that affects protein secretion in Saccharomyces cerevisiae." Chow T.Y.-K., Ash J.J., Dignard D., Thomas D.Y. J. Cell Sci. 101:709-719(1992) [PubMed: 1522152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Functional and physical interactions between autonomously replicating sequence-binding factor 1 and the nuclear transport machinery." Loch C.M., Mosammaparast N., Miyake T., Pemberton L.F., Li R. Traffic 5:925-935(2004) [PubMed: 15522095] [Abstract] Cited for: INTERACTION WITH ABF1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11538 Genomic DNA. Translation: CAA77639.1. S45357 Genomic DNA. Translation: AAA10665.1. Z49212 Genomic DNA. Translation: CAA89141.1. BK006946 Genomic DNA. Translation: DAA10209.1.
PIR	S53978.
RefSeq	NP_014039.1. NM_001182819.1.
3D structure databases
ProteinModelPortal	P32337.
SMR	P32337. Positions 138-165, 175-208, 378-544, 900-932.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1533N.
IntAct	P32337. 78 interactions.
MINT	MINT-390897.
STRING	P32337.
Proteomic databases
PeptideAtlas	P32337.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR308C; YMR308C; YMR308C.
GeneID	855356.
KEGG	sce:YMR308C.
NMPDR	fig\|4932.3.peg.5098.
Organism-specific databases
CYGD	YMR308c.
SGD	S000004925. PSE1.
Phylogenomic databases
eggNOG	fuNOG05809.
GeneTree	EFGT00050000005847.
HOGENOM	HBG378569.
OMA	EWESVES.
OrthoDB	EOG4B5SD7.
Gene expression databases
ArrayExpress	P32337.
Genevestigator	P32337.
GermOnline	YMR308C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 3 hits.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50077. HEAT_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	979118.

Entry information

Entry name

IMB3_YEAST

Accession

Primary (citable) accession number: P32337
Secondary accession number(s): D6W0D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents