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Protein

Importin subunit beta-3

Gene

PSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for classical and arginine/glycine-rich nuclear localization signals (cNLS and rg-NLS) in cargo substrates (PubMed:15367670). Its predominant cargo substrate seems to be ribosomal proteins and ribosome biogenesis trans- and cis-acting factors (PubMed:9182759, PubMed:9321403, PubMed:15367670). Required for nuclear transport of YAP1, NOP1 and SOF1 (PubMed:11274141, PubMed:15367670). Mediates the nuclear import of histones H3 and H4 (PubMed:11694505). Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins. The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11423015). At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo (PubMed:9321403). The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11423015).1 Publication5 Publications
Plays a role in protein secretion.1 Publication

GO - Molecular functioni

  • nuclear localization sequence binding Source: SGD
  • protein transporter activity Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • NLS-bearing protein import into nucleus Source: GO_Central
  • protein import into nucleus Source: SGD
  • protein import into nucleus, docking Source: GO_Central
  • protein import into nucleus, translocation Source: GO_Central
  • regulation of mitotic nuclear division Source: SGD
  • regulation of protein desumoylation Source: SGD
  • ribosomal protein import into nucleus Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32972-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-3Curated
Alternative name(s):
Karyopherin subunit beta-3Curated
Karyopherin-1211 Publication
Protein secretion enhancer 11 Publication
Gene namesi
Name:PSE11 Publication
Synonyms:KAP1211 Publication
Ordered Locus Names:YMR308CImported
ORF Names:YM9952.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR308C.
SGDiS000004925. PSE1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear membrane Source: GO_Central
  • nuclear periphery Source: GO_Central
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001207742 – 1089Importin subunit beta-3Add BLAST1088

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei830PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32337.
PRIDEiP32337.

PTM databases

iPTMnetiP32337.

Interactioni

Subunit structurei

Interacts with Ran (GSP1); interacts specifically with the GTP-bound form of Ran (GTP-Ran), protecting it from GTP hydrolysis and nucleotide exchange (PubMed:9321403). Interacts with RPL25; this interaction is dissociated by binding to Ran-GTP (PubMed:9321403). Interacts with YAP1; this interaction is dissociated by binding to Ran-GTP (PubMed:11274141). Interacts with NOP1; via its rg-NLS (PubMed:15367670). Interacts with SOF1; via its cNLS (PubMed:15367670). Interacts with histones H3 and H4; via their NLS (PubMed:11694505). Interacts with ABF1 (PubMed:15522095).4 Publications

Protein-protein interaction databases

BioGridi35488. 157 interactors.
DIPiDIP-1533N.
IntActiP32337. 73 interactors.
MINTiMINT-390897.

Structurei

Secondary structure

11089
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 18Combined sources13
Helixi25 – 37Combined sources13
Beta strandi38 – 40Combined sources3
Turni41 – 43Combined sources3
Helixi44 – 57Combined sources14
Helixi61 – 77Combined sources17
Helixi91 – 93Combined sources3
Helixi96 – 111Combined sources16
Helixi116 – 127Combined sources12
Helixi138 – 149Combined sources12
Helixi153 – 165Combined sources13
Helixi168 – 172Combined sources5
Helixi175 – 185Combined sources11
Helixi191 – 207Combined sources17
Helixi210 – 215Combined sources6
Helixi217 – 219Combined sources3
Helixi220 – 226Combined sources7
Helixi228 – 232Combined sources5
Helixi236 – 252Combined sources17
Helixi254 – 260Combined sources7
Helixi261 – 273Combined sources13
Helixi279 – 295Combined sources17
Helixi297 – 301Combined sources5
Helixi304 – 318Combined sources15
Helixi329 – 332Combined sources4
Helixi343 – 359Combined sources17
Helixi361 – 376Combined sources16
Helixi381 – 394Combined sources14
Turni395 – 398Combined sources4
Helixi399 – 402Combined sources4
Helixi406 – 413Combined sources8
Helixi414 – 418Combined sources5
Helixi422 – 438Combined sources17
Turni439 – 441Combined sources3
Helixi442 – 458Combined sources17
Helixi465 – 480Combined sources16
Helixi484 – 487Combined sources4
Helixi488 – 490Combined sources3
Helixi491 – 502Combined sources12
Helixi507 – 524Combined sources18
Helixi525 – 531Combined sources7
Helixi532 – 544Combined sources13
Helixi551 – 568Combined sources18
Helixi570 – 573Combined sources4
Turni574 – 576Combined sources3
Helixi577 – 588Combined sources12
Helixi597 – 613Combined sources17
Helixi614 – 620Combined sources7
Helixi621 – 632Combined sources12
Beta strandi638 – 641Combined sources4
Beta strandi646 – 648Combined sources3
Helixi649 – 651Combined sources3
Beta strandi655 – 668Combined sources14
Helixi669 – 689Combined sources21
Helixi690 – 693Combined sources4
Helixi694 – 703Combined sources10
Helixi705 – 709Combined sources5
Helixi715 – 734Combined sources20
Helixi742 – 757Combined sources16
Turni758 – 760Combined sources3
Helixi764 – 781Combined sources18
Helixi788 – 809Combined sources22
Helixi829 – 849Combined sources21
Turni850 – 853Combined sources4
Helixi854 – 857Combined sources4
Helixi858 – 860Combined sources3
Helixi861 – 868Combined sources8
Helixi873 – 887Combined sources15
Helixi891 – 893Combined sources3
Helixi895 – 909Combined sources15
Helixi914 – 930Combined sources17
Turni933 – 935Combined sources3
Helixi936 – 949Combined sources14
Helixi958 – 960Combined sources3
Helixi961 – 977Combined sources17
Turni978 – 980Combined sources3
Helixi987 – 993Combined sources7
Helixi1002 – 1013Combined sources12
Helixi1029 – 1041Combined sources13
Helixi1053 – 1062Combined sources10
Helixi1066 – 1071Combined sources6
Helixi1072 – 1075Combined sources4
Helixi1078 – 1085Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W3TX-ray2.90A1-1089[»]
3W3UX-ray2.60A1-1089[»]
3W3VX-ray3.20A1-1089[»]
3W3WX-ray2.20A1-1089[»]
3W3XX-ray2.90A1-1089[»]
3W3YX-ray2.80A1-1089[»]
3W3ZX-ray2.70A1-1089[»]
4ZJ7X-ray2.40A1-1089[»]
ProteinModelPortaliP32337.
SMRiP32337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati6 – 39HEAT 11 PublicationAdd BLAST34
Repeati44 – 78HEAT 21 PublicationAdd BLAST35
Repeati96 – 129HEAT 31 PublicationAdd BLAST34
Repeati138 – 165HEAT 41 PublicationAdd BLAST28
Repeati175 – 207HEAT 51 PublicationAdd BLAST33
Repeati216 – 252HEAT 61 PublicationAdd BLAST37
Repeati260 – 295HEAT 71 PublicationAdd BLAST36
Repeati304 – 359HEAT 81 PublicationAdd BLAST56
Repeati361 – 395HEAT 91 PublicationAdd BLAST35
Repeati399 – 439HEAT 101 PublicationAdd BLAST41
Repeati441 – 481HEAT 111 PublicationAdd BLAST41
Repeati484 – 524HEAT 121 PublicationAdd BLAST41
Repeati526 – 568HEAT 131 PublicationAdd BLAST43
Repeati571 – 613HEAT 141 PublicationAdd BLAST43
Repeati615 – 689HEAT 151 PublicationAdd BLAST75
Repeati692 – 735HEAT 161 PublicationAdd BLAST44
Repeati742 – 781HEAT 171 PublicationAdd BLAST40
Repeati788 – 849HEAT 181 PublicationAdd BLAST62
Repeati852 – 890HEAT 191 PublicationAdd BLAST39
Repeati898 – 930HEAT 201 PublicationAdd BLAST33
Repeati938 – 978HEAT 211 PublicationAdd BLAST41
Repeati986 – 1017HEAT 221 PublicationAdd BLAST32
Repeati1028 – 1063HEAT 231 PublicationAdd BLAST36
Repeati1066 – 1089HEAT 241 PublicationAdd BLAST24

Sequence similaritiesi

Contains 24 HEAT repeats.1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074773.
HOGENOMiHOG000209725.
InParanoidiP32337.
KOiK20222.
OMAiIERITHM.
OrthoDBiEOG092C0HC0.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32337-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALPEEVNR TLLQIVQAFA SPDNQIRSVA EKALSEEWIT ENNIEYLLTF
60 70 80 90 100
LAEQAAFSQD TTVAALSAVL FRKLALKAPP SSKLMIMSKN ITHIRKEVLA
110 120 130 140 150
QIRSSLLKGF LSERADSIRH KLSDAIAECV QDDLPAWPEL LQALIESLKS
160 170 180 190 200
GNPNFRESSF RILTTVPYLI TAVDINSILP IFQSGFTDAS DNVKIAAVTA
210 220 230 240 250
FVGYFKQLPK SEWSKLGILL PSLLNSLPRF LDDGKDDALA SVFESLIELV
260 270 280 290 300
ELAPKLFKDM FDQIIQFTDM VIKNKDLEPP ARTTALELLT VFSENAPQMC
310 320 330 340 350
KSNQNYGQTL VMVTLIMMTE VSIDDDDAAE WIESDDTDDE EEVTYDHARQ
360 370 380 390 400
ALDRVALKLG GEYLAAPLFQ YLQQMITSTE WRERFAAMMA LSSAAEGCAD
410 420 430 440 450
VLIGEIPKIL DMVIPLINDP HPRVQYGCCN VLGQISTDFS PFIQRTAHDR
460 470 480 490 500
ILPALISKLT SECTSRVQTH AAAALVNFSE FASKDILEPY LDSLLTNLLV
510 520 530 540 550
LLQSNKLYVQ EQALTTIAFI AEAAKNKFIK YYDTLMPLLL NVLKVNNKDN
560 570 580 590 600
SVLKGKCMEC ATLIGFAVGK EKFHEHSQEL ISILVALQNS DIDEDDALRS
610 620 630 640 650
YLEQSWSRIC RILGDDFVPL LPIVIPPLLI TAKATQDVGL IEEEEAANFQ
660 670 680 690 700
QYPDWDVVQV QGKHIAIHTS VLDDKVSAME LLQSYATLLR GQFAVYVKEV
710 720 730 740 750
MEEIALPSLD FYLHDGVRAA GATLIPILLS CLLAATGTQN EELVLLWHKA
760 770 780 790 800
SSKLIGGLMS EPMPEITQVY HNSLVNGIKV MGDNCLSEDQ LAAFTKGVSA
810 820 830 840 850
NLTDTYERMQ DRHGDGDEYN ENIDEEEDFT DEDLLDEINK SIAAVLKTTN
860 870 880 890 900
GHYLKNLENI WPMINTFLLD NEPILVIFAL VVIGDLIQYG GEQTASMKNA
910 920 930 940 950
FIPKVTECLI SPDARIRQAA SYIIGVCAQY APSTYADVCI PTLDTLVQIV
960 970 980 990 1000
DFPGSKLEEN RSSTENASAA IAKILYAYNS NIPNVDTYTA NWFKTLPTIT
1010 1020 1030 1040 1050
DKEAASFNYQ FLSQLIENNS PIVCAQSNIS AVVDSVIQAL NERSLTEREG
1060 1070 1080
QTVISSVKKL LGFLPSSDAM AIFNRYPADI MEKVHKWFA
Length:1,089
Mass (Da):121,031
Last modified:October 1, 1996 - v2
Checksum:i9C5F7FB5B8824C37
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65A → S in CAA77639 (PubMed:1522152).Curated1
Sequence conflicti65A → S in AAA10665 (PubMed:1522152).Curated1
Sequence conflicti1077P → A in CAA77639 (PubMed:1522152).Curated1
Sequence conflicti1077P → A in AAA10665 (PubMed:1522152).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11538 Genomic DNA. Translation: CAA77639.1.
S45357 Genomic DNA. Translation: AAA10665.1.
Z49212 Genomic DNA. Translation: CAA89141.1.
BK006946 Genomic DNA. Translation: DAA10209.1.
PIRiS53978.
RefSeqiNP_014039.1. NM_001182819.1.

Genome annotation databases

EnsemblFungiiYMR308C; YMR308C; YMR308C.
GeneIDi855356.
KEGGisce:YMR308C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11538 Genomic DNA. Translation: CAA77639.1.
S45357 Genomic DNA. Translation: AAA10665.1.
Z49212 Genomic DNA. Translation: CAA89141.1.
BK006946 Genomic DNA. Translation: DAA10209.1.
PIRiS53978.
RefSeqiNP_014039.1. NM_001182819.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3W3TX-ray2.90A1-1089[»]
3W3UX-ray2.60A1-1089[»]
3W3VX-ray3.20A1-1089[»]
3W3WX-ray2.20A1-1089[»]
3W3XX-ray2.90A1-1089[»]
3W3YX-ray2.80A1-1089[»]
3W3ZX-ray2.70A1-1089[»]
4ZJ7X-ray2.40A1-1089[»]
ProteinModelPortaliP32337.
SMRiP32337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35488. 157 interactors.
DIPiDIP-1533N.
IntActiP32337. 73 interactors.
MINTiMINT-390897.

PTM databases

iPTMnetiP32337.

Proteomic databases

MaxQBiP32337.
PRIDEiP32337.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR308C; YMR308C; YMR308C.
GeneIDi855356.
KEGGisce:YMR308C.

Organism-specific databases

EuPathDBiFungiDB:YMR308C.
SGDiS000004925. PSE1.

Phylogenomic databases

GeneTreeiENSGT00550000074773.
HOGENOMiHOG000209725.
InParanoidiP32337.
KOiK20222.
OMAiIERITHM.
OrthoDBiEOG092C0HC0.

Enzyme and pathway databases

BioCyciYEAST:G3O-32972-MONOMER.

Miscellaneous databases

PROiP32337.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiIMB3_YEAST
AccessioniPrimary (citable) accession number: P32337
Secondary accession number(s): D6W0D5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.