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P32337

- IMB3_YEAST

UniProt

P32337 - IMB3_YEAST

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Protein

Importin subunit beta-3

Gene

PSE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the nuclear import of ribosomal proteins. Binds to nucleoporins and the GTP-bound form of GSP1 (Ran).
Plays a role in protein secretion.

GO - Molecular functioni

  1. nuclear localization sequence binding Source: SGD
  2. protein transporter activity Source: SGD

GO - Biological processi

  1. mRNA export from nucleus Source: SGD
  2. protein import into nucleus Source: SGD
  3. regulation of mitosis Source: SGD
  4. regulation of protein desumoylation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32972-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-3
Alternative name(s):
Karyopherin subunit beta-3
Karyopherin-121
Protein secretion enhancer 1
Gene namesi
Name:PSE1
Synonyms:KAP121
Ordered Locus Names:YMR308C
ORF Names:YM9952.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR308c.
SGDiS000004925. PSE1.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 10891088Importin subunit beta-3PRO_0000120774Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei830 – 8301Phosphothreonine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32337.
PaxDbiP32337.
PeptideAtlasiP32337.

Expressioni

Gene expression databases

GenevestigatoriP32337.

Interactioni

Subunit structurei

Interacts with ABF1.1 Publication

Protein-protein interaction databases

BioGridi35488. 152 interactions.
DIPiDIP-1533N.
IntActiP32337. 73 interactions.
MINTiMINT-390897.
STRINGi4932.YMR308C.

Structurei

Secondary structure

1
1089
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1813
Helixi25 – 3713
Turni41 – 433
Helixi44 – 5714
Helixi61 – 7717
Helixi91 – 933
Helixi96 – 11116
Helixi116 – 12712
Helixi138 – 14912
Helixi153 – 16513
Helixi168 – 1725
Helixi175 – 18511
Helixi191 – 20717
Helixi210 – 2156
Helixi217 – 2193
Helixi220 – 2267
Helixi228 – 2325
Helixi236 – 25217
Helixi254 – 2607
Helixi261 – 27313
Helixi279 – 29517
Helixi297 – 3015
Helixi304 – 31815
Helixi329 – 3324
Helixi343 – 35917
Helixi361 – 37616
Helixi381 – 39414
Turni395 – 3984
Helixi399 – 4024
Helixi406 – 4138
Helixi414 – 4185
Helixi422 – 43817
Turni439 – 4413
Helixi442 – 45817
Helixi465 – 48016
Helixi484 – 4874
Helixi488 – 4903
Helixi491 – 50212
Helixi507 – 52418
Helixi525 – 5317
Helixi532 – 54413
Helixi551 – 56818
Helixi570 – 5734
Turni574 – 5763
Helixi577 – 58812
Helixi597 – 61317
Helixi614 – 6207
Helixi621 – 63212
Beta strandi638 – 6414
Beta strandi646 – 6483
Helixi649 – 6513
Beta strandi655 – 66814
Helixi669 – 68921
Helixi690 – 6934
Helixi694 – 70310
Helixi705 – 7095
Helixi715 – 73420
Helixi742 – 75716
Turni758 – 7603
Helixi764 – 78118
Helixi788 – 80922
Helixi829 – 84921
Turni850 – 8534
Helixi854 – 8574
Helixi858 – 8603
Helixi861 – 8688
Helixi873 – 88715
Helixi891 – 8933
Helixi895 – 90915
Helixi914 – 93017
Turni933 – 9353
Helixi936 – 94914
Helixi958 – 9603
Helixi961 – 97717
Turni978 – 9803
Helixi987 – 9937
Helixi1002 – 101312
Helixi1029 – 104113
Helixi1053 – 106210
Helixi1066 – 10716
Helixi1072 – 10754
Helixi1078 – 10858

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3W3TX-ray2.90A1-1089[»]
3W3UX-ray2.60A1-1089[»]
3W3VX-ray3.20A1-1089[»]
3W3WX-ray2.20A1-1089[»]
3W3XX-ray2.90A1-1089[»]
3W3YX-ray2.80A1-1089[»]
3W3ZX-ray2.70A1-1089[»]
ProteinModelPortaliP32337.
SMRiP32337. Positions 133-549.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati6 – 4338HEAT 1Add
BLAST
Repeati135 – 17238HEAT 2Add
BLAST
Repeati174 – 21037HEAT 3Add
BLAST
Repeati404 – 44138HEAT 4Add
BLAST
Repeati446 – 48439HEAT 5Add
BLAST
Repeati489 – 52638HEAT 6Add
BLAST
Repeati530 – 57041HEAT 7Add
BLAST
Repeati719 – 75638HEAT 8Add
BLAST
Repeati896 – 93338HEAT 9Add
BLAST

Sequence similaritiesi

Belongs to the importin beta family.Curated
Contains 9 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG249123.
GeneTreeiENSGT00550000074773.
HOGENOMiHOG000209725.
InParanoidiP32337.
OMAiNCNLPRI.
OrthoDBiEOG7WX0HQ.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32337-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSALPEEVNR TLLQIVQAFA SPDNQIRSVA EKALSEEWIT ENNIEYLLTF
60 70 80 90 100
LAEQAAFSQD TTVAALSAVL FRKLALKAPP SSKLMIMSKN ITHIRKEVLA
110 120 130 140 150
QIRSSLLKGF LSERADSIRH KLSDAIAECV QDDLPAWPEL LQALIESLKS
160 170 180 190 200
GNPNFRESSF RILTTVPYLI TAVDINSILP IFQSGFTDAS DNVKIAAVTA
210 220 230 240 250
FVGYFKQLPK SEWSKLGILL PSLLNSLPRF LDDGKDDALA SVFESLIELV
260 270 280 290 300
ELAPKLFKDM FDQIIQFTDM VIKNKDLEPP ARTTALELLT VFSENAPQMC
310 320 330 340 350
KSNQNYGQTL VMVTLIMMTE VSIDDDDAAE WIESDDTDDE EEVTYDHARQ
360 370 380 390 400
ALDRVALKLG GEYLAAPLFQ YLQQMITSTE WRERFAAMMA LSSAAEGCAD
410 420 430 440 450
VLIGEIPKIL DMVIPLINDP HPRVQYGCCN VLGQISTDFS PFIQRTAHDR
460 470 480 490 500
ILPALISKLT SECTSRVQTH AAAALVNFSE FASKDILEPY LDSLLTNLLV
510 520 530 540 550
LLQSNKLYVQ EQALTTIAFI AEAAKNKFIK YYDTLMPLLL NVLKVNNKDN
560 570 580 590 600
SVLKGKCMEC ATLIGFAVGK EKFHEHSQEL ISILVALQNS DIDEDDALRS
610 620 630 640 650
YLEQSWSRIC RILGDDFVPL LPIVIPPLLI TAKATQDVGL IEEEEAANFQ
660 670 680 690 700
QYPDWDVVQV QGKHIAIHTS VLDDKVSAME LLQSYATLLR GQFAVYVKEV
710 720 730 740 750
MEEIALPSLD FYLHDGVRAA GATLIPILLS CLLAATGTQN EELVLLWHKA
760 770 780 790 800
SSKLIGGLMS EPMPEITQVY HNSLVNGIKV MGDNCLSEDQ LAAFTKGVSA
810 820 830 840 850
NLTDTYERMQ DRHGDGDEYN ENIDEEEDFT DEDLLDEINK SIAAVLKTTN
860 870 880 890 900
GHYLKNLENI WPMINTFLLD NEPILVIFAL VVIGDLIQYG GEQTASMKNA
910 920 930 940 950
FIPKVTECLI SPDARIRQAA SYIIGVCAQY APSTYADVCI PTLDTLVQIV
960 970 980 990 1000
DFPGSKLEEN RSSTENASAA IAKILYAYNS NIPNVDTYTA NWFKTLPTIT
1010 1020 1030 1040 1050
DKEAASFNYQ FLSQLIENNS PIVCAQSNIS AVVDSVIQAL NERSLTEREG
1060 1070 1080
QTVISSVKKL LGFLPSSDAM AIFNRYPADI MEKVHKWFA
Length:1,089
Mass (Da):121,031
Last modified:October 1, 1996 - v2
Checksum:i9C5F7FB5B8824C37
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651A → S in CAA77639. (PubMed:1522152)Curated
Sequence conflicti65 – 651A → S in AAA10665. (PubMed:1522152)Curated
Sequence conflicti1077 – 10771P → A in CAA77639. (PubMed:1522152)Curated
Sequence conflicti1077 – 10771P → A in AAA10665. (PubMed:1522152)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11538 Genomic DNA. Translation: CAA77639.1.
S45357 Genomic DNA. Translation: AAA10665.1.
Z49212 Genomic DNA. Translation: CAA89141.1.
BK006946 Genomic DNA. Translation: DAA10209.1.
PIRiS53978.
RefSeqiNP_014039.1. NM_001182819.1.

Genome annotation databases

EnsemblFungiiYMR308C; YMR308C; YMR308C.
GeneIDi855356.
KEGGisce:YMR308C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11538 Genomic DNA. Translation: CAA77639.1 .
S45357 Genomic DNA. Translation: AAA10665.1 .
Z49212 Genomic DNA. Translation: CAA89141.1 .
BK006946 Genomic DNA. Translation: DAA10209.1 .
PIRi S53978.
RefSeqi NP_014039.1. NM_001182819.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3W3T X-ray 2.90 A 1-1089 [» ]
3W3U X-ray 2.60 A 1-1089 [» ]
3W3V X-ray 3.20 A 1-1089 [» ]
3W3W X-ray 2.20 A 1-1089 [» ]
3W3X X-ray 2.90 A 1-1089 [» ]
3W3Y X-ray 2.80 A 1-1089 [» ]
3W3Z X-ray 2.70 A 1-1089 [» ]
ProteinModelPortali P32337.
SMRi P32337. Positions 133-549.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35488. 152 interactions.
DIPi DIP-1533N.
IntActi P32337. 73 interactions.
MINTi MINT-390897.
STRINGi 4932.YMR308C.

Proteomic databases

MaxQBi P32337.
PaxDbi P32337.
PeptideAtlasi P32337.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR308C ; YMR308C ; YMR308C .
GeneIDi 855356.
KEGGi sce:YMR308C.

Organism-specific databases

CYGDi YMR308c.
SGDi S000004925. PSE1.

Phylogenomic databases

eggNOGi NOG249123.
GeneTreei ENSGT00550000074773.
HOGENOMi HOG000209725.
InParanoidi P32337.
OMAi NCNLPRI.
OrthoDBi EOG7WX0HQ.

Enzyme and pathway databases

BioCyci YEAST:G3O-32972-MONOMER.

Miscellaneous databases

NextBioi 979118.

Gene expression databases

Genevestigatori P32337.

Family and domain databases

Gene3Di 1.25.10.10. 2 hits.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Screening and identification of a gene, PSE-1, that affects protein secretion in Saccharomyces cerevisiae."
    Chow T.Y.-K., Ash J.J., Dignard D., Thomas D.Y.
    J. Cell Sci. 101:709-719(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Functional and physical interactions between autonomously replicating sequence-binding factor 1 and the nuclear transport machinery."
    Loch C.M., Mosammaparast N., Miyake T., Pemberton L.F., Li R.
    Traffic 5:925-935(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ABF1.
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-830, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIMB3_YEAST
AccessioniPrimary (citable) accession number: P32337
Secondary accession number(s): D6W0D5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3