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Protein

Signaling mucin MSB2

Gene

MSB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane signaling mucin that promotes activation of the MAPK for the filamentous growth pathway. Partially redundant with the SHO1 osmosensing branch for the activation of STE11.6 Publications

GO - Molecular functioni

  • osmosensor activity Source: SGD

GO - Biological processi

  • cellular bud site selection Source: GO_Central
  • establishment of cell polarity Source: SGD
  • fungal-type cell wall organization Source: GO_Central
  • hyperosmotic response Source: SGD
  • osmosensory signaling pathway via Sho1 osmosensor Source: SGD
  • response to osmotic stress Source: SGD
  • signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30741-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signaling mucin MSB2
Alternative name(s):
Multicopy suppressor of bud emergence 2
Osmosensor MSB2
Gene namesi
Name:MSB2
Ordered Locus Names:YGR014W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR014W.
SGDiS000003246. MSB2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 11851164ExtracellularSequence analysisAdd
BLAST
Transmembranei1186 – 120621HelicalSequence analysisAdd
BLAST
Topological domaini1207 – 1306100CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: GO_Central
  • extracellular region Source: SGD
  • integral component of plasma membrane Source: SGD
  • site of polarized growth Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 13061285Signaling mucin MSB2PRO_0000096589Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi30 – 301N-linked (GlcNAc...)Sequence analysis
Glycosylationi859 – 8591N-linked (GlcNAc...)Sequence analysis
Glycosylationi885 – 8851N-linked (GlcNAc...)Sequence analysis
Glycosylationi945 – 9451N-linked (GlcNAc...)Sequence analysis
Glycosylationi1049 – 10491N-linked (GlcNAc...)Sequence analysis
Glycosylationi1088 – 10881N-linked (GlcNAc...)Sequence analysis
Glycosylationi1175 – 11751N-linked (GlcNAc...)Sequence analysis
Modified residuei1300 – 13001PhosphoserineCombined sources

Post-translational modificationi

O-glycosylated in the Ser/Thr-rich regions.Curated

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32334.

PTM databases

iPTMnetiP32334.

Interactioni

Subunit structurei

Interacts with CDC42 and SHO1.1 Publication

Protein-protein interaction databases

BioGridi33257. 53 interactions.
DIPiDIP-1455N.
IntActiP32334. 12 interactions.
MINTiMINT-396603.

Structurei

3D structure databases

ProteinModelPortaliP32334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati698 – 714171Add
BLAST
Repeati715 – 731172Add
BLAST
Repeati732 – 748173Add
BLAST
Repeati749 – 765174Add
BLAST
Repeati766 – 782175Add
BLAST
Repeati783 – 799176Add
BLAST
Repeati800 – 816177Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni698 – 8161197 X 17 AA tandem repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi37 – 956920Ser-richAdd
BLAST
Compositional biasi1132 – 115019Poly-SerAdd
BLAST

Sequence similaritiesi

Belongs to the HKR1/MSB2 family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00770000121599.
InParanoidiP32334.
KOiK19849.
OMAiCAHEESS.
OrthoDBiEOG78H456.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFPFACLLS TLVISGSLAR ASPFDFIFGN GTQQAQSQSE SQGQVSFTNE
60 70 80 90 100
ASQDSSTTSL VTAYSQGVHS HQSATIVSAT ISSLPSTWYD ASSTSQTSVS
110 120 130 140 150
YASQESDYAV NQNSWSASTN QLPSTSTTSY YAPTFSTSAD FAASSVNAAS
160 170 180 190 200
DVSTASVPID TSANSIPFTT TSNIETTTSA PLTSDTPLIS TSTMSAADNV
210 220 230 240 250
FSSANPISAS LTTTDSSESF DQTSTAGAIP VQSSADFSSS SEILVQSSAD
260 270 280 290 300
FSSPSSPTTT DISLSAAPLQ TSESSSFTTA SAALPVSSTD VDGSSASPVV
310 320 330 340 350
SMSAAGQIAS SSSTDNPTMS ETFSLTSTEV DGSDVSSTVS ALLSAPFLQT
360 370 380 390 400
STSNSFSIVS PSVSFVPSQS SSDVASSSTA NVVSSSFSDI PPQTSTSGSV
410 420 430 440 450
VSVAQSASAL AFQSSTEVYG ASASSTMSSL LSTTSLQSTT LDSSSLASSS
460 470 480 490 500
ASSSDLTDYG VSSTASIPLL SASEQASTSS SFSVVSPSVS FVPSQSSSDV
510 520 530 540 550
ASTSAPSVVS SSFSYTSLQA GGSSMTNPSS STIVYSSSTG SSEESAASTA
560 570 580 590 600
SATLSGSSST YMAGNLQSQP PSTSSLLSES QATSTSAVLA SSSVSTTSPY
610 620 630 640 650
TTAGGASTEA SSLISSTSAE TSQVSYSQST TALQTSSFAS SSTTEGSETS
660 670 680 690 700
SQGFSTSSVL VQMPSSISSE FSPSQTTTQM NSASSSSQYT ISSTGILSQV
710 720 730 740 750
SDTSVSYTTS SSSVSQVSDT PVSYTTSSSS VSQVSDTPVS YTTSSSSVSQ
760 770 780 790 800
VSDTPVSYTT SSSSVSQVSD TPVSYTTSSS SVSQVSDTSV PSTSSRSSVS
810 820 830 840 850
QVSDTPVPST SSRSSVSQTS SSLQPTTTSS QRFTISTHGA LSESSSVSQQ
860 870 880 890 900
ASEITSSINA TASEYHSIQT TAATQSTTLS FTDANSSSAS APLEVATSTP
910 920 930 940 950
TPSSKASSLL LTPSTSSLSQ VATNTNVQTS LTTESTTVLE PSTTNSSSTF
960 970 980 990 1000
SLVTSSDNNW WIPTELITQA PEAASTASST VGGTQTMTLP HAIAAATQVP
1010 1020 1030 1040 1050
EPEGYTLITI GFKKALNYEF VVSEPKSSAQ IFGYLPEALN TPFKNVFTNI
1060 1070 1080 1090 1100
TVLQIVPLQD DSLNYLVSVA EVYFPTAEIE ELSNLITNSS SAFYTDGMGT
1110 1120 1130 1140 1150
AKSMAAMVDS SIPLTGLLHD SNSNSGGSSD GSSSSNSNSG SSGSGSNSNS
1160 1170 1180 1190 1200
GVSSSSGNSY QDAGTLEYSS KSNSNVSTSS KSKKKIIGLV IGVVVGGCLY
1210 1220 1230 1240 1250
ILFMIFAFKY IIRRRIQSQE IIKNPEISSI SSSEFGGEKN YNNEKRMSVQ
1260 1270 1280 1290 1300
ESITQSMRIQ NWMDDSYYGH GLTNNDSTPT RHNTSSSIPK ISRPIASQNS

LGWNEV
Length:1,306
Mass (Da):133,115
Last modified:October 1, 1993 - v1
Checksum:i67D5D984D5CA4A6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77354 Genomic DNA. Translation: AAA34798.1.
Z72799 Genomic DNA. Translation: CAA96997.1.
BK006941 Genomic DNA. Translation: DAA08111.1.
PIRiS25370.
RefSeqiNP_011528.3. NM_001181143.3.

Genome annotation databases

EnsemblFungiiYGR014W; YGR014W; YGR014W.
GeneIDi852897.
KEGGisce:YGR014W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77354 Genomic DNA. Translation: AAA34798.1.
Z72799 Genomic DNA. Translation: CAA96997.1.
BK006941 Genomic DNA. Translation: DAA08111.1.
PIRiS25370.
RefSeqiNP_011528.3. NM_001181143.3.

3D structure databases

ProteinModelPortaliP32334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33257. 53 interactions.
DIPiDIP-1455N.
IntActiP32334. 12 interactions.
MINTiMINT-396603.

PTM databases

iPTMnetiP32334.

Proteomic databases

MaxQBiP32334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR014W; YGR014W; YGR014W.
GeneIDi852897.
KEGGisce:YGR014W.

Organism-specific databases

EuPathDBiFungiDB:YGR014W.
SGDiS000003246. MSB2.

Phylogenomic databases

GeneTreeiENSGT00770000121599.
InParanoidiP32334.
KOiK19849.
OMAiCAHEESS.
OrthoDBiEOG78H456.

Enzyme and pathway databases

BioCyciYEAST:G3O-30741-MONOMER.

Miscellaneous databases

PROiP32334.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A Ser/Thr-rich multicopy suppressor of a cdc24 bud emergence defect."
    Bender A., Pringle J.R.
    Yeast 8:315-323(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1."
    Bender A., Pringle J.R.
    Proc. Natl. Acad. Sci. U.S.A. 86:9976-9980(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A third osmosensing branch in Saccharomyces cerevisiae requires the Msb2 protein and functions in parallel with the Sho1 branch."
    O'Rourke S.M., Herskowitz I.
    Mol. Cell. Biol. 22:4739-4749(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast."
    Cullen P.J., Sabbagh W. Jr., Graham E., Irick M.M., van Olden E.K., Neal C., Delrow J., Bardwell L., Sprague G.F. Jr.
    Genes Dev. 18:1695-1708(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CDC42 AND SHO1.
  9. "Mitogen-activated protein kinases with distinct requirements for Ste5 scaffolding influence signaling specificity in Saccharomyces cerevisiae."
    Flatauer L.J., Zadeh S.F., Bardwell L.
    Mol. Cell. Biol. 25:1793-1803(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response."
    Pitoniak A., Birkaya B., Dionne H.M., Vadaie N., Cullen P.J.
    Mol. Biol. Cell 20:3101-3114(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1300, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMSB2_YEAST
AccessioniPrimary (citable) accession number: P32334
Secondary accession number(s): D6VUF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 6, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1320 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.