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Protein

Signaling mucin MSB2

Gene

MSB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane signaling mucin that promotes activation of the MAPK for the filamentous growth pathway. Partially redundant with the SHO1 osmosensing branch for the activation of STE11.6 Publications

GO - Molecular functioni

  • osmosensor activity Source: SGD

GO - Biological processi

  • cellular bud site selection Source: GO_Central
  • establishment of cell polarity Source: SGD
  • fungal-type cell wall organization Source: GO_Central
  • hyperosmotic response Source: SGD
  • osmosensory signaling pathway via Sho1 osmosensor Source: SGD
  • response to osmotic stress Source: SGD
  • signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30741-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signaling mucin MSB2
Alternative name(s):
Multicopy suppressor of bud emergence 2
Osmosensor MSB2
Gene namesi
Name:MSB2
Ordered Locus Names:YGR014W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR014W.
SGDiS000003246. MSB2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 1185ExtracellularSequence analysisAdd BLAST1164
Transmembranei1186 – 1206HelicalSequence analysisAdd BLAST21
Topological domaini1207 – 1306CytoplasmicSequence analysisAdd BLAST100

GO - Cellular componenti

  • cell surface Source: GO_Central
  • extracellular region Source: SGD
  • integral component of plasma membrane Source: SGD
  • site of polarized growth Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000009658922 – 1306Signaling mucin MSB2Add BLAST1285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi30N-linked (GlcNAc...)Sequence analysis1
Glycosylationi859N-linked (GlcNAc...)Sequence analysis1
Glycosylationi885N-linked (GlcNAc...)Sequence analysis1
Glycosylationi945N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1049N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1088N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1175N-linked (GlcNAc...)Sequence analysis1
Modified residuei1300PhosphoserineCombined sources1

Post-translational modificationi

O-glycosylated in the Ser/Thr-rich regions.Curated

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP32334.
PRIDEiP32334.

PTM databases

iPTMnetiP32334.

Interactioni

Subunit structurei

Interacts with CDC42 and SHO1.1 Publication

Protein-protein interaction databases

BioGridi33257. 53 interactors.
DIPiDIP-1455N.
IntActiP32334. 12 interactors.
MINTiMINT-396603.

Structurei

3D structure databases

ProteinModelPortaliP32334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati698 – 7141Add BLAST17
Repeati715 – 7312Add BLAST17
Repeati732 – 7483Add BLAST17
Repeati749 – 7654Add BLAST17
Repeati766 – 7825Add BLAST17
Repeati783 – 7996Add BLAST17
Repeati800 – 8167Add BLAST17

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni698 – 8167 X 17 AA tandem repeatsAdd BLAST119

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi37 – 956Ser-richAdd BLAST920
Compositional biasi1132 – 1150Poly-SerAdd BLAST19

Sequence similaritiesi

Belongs to the HKR1/MSB2 family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00770000121599.
InParanoidiP32334.
KOiK19849.
OMAiSLGWNEV.
OrthoDBiEOG092C480G.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFPFACLLS TLVISGSLAR ASPFDFIFGN GTQQAQSQSE SQGQVSFTNE
60 70 80 90 100
ASQDSSTTSL VTAYSQGVHS HQSATIVSAT ISSLPSTWYD ASSTSQTSVS
110 120 130 140 150
YASQESDYAV NQNSWSASTN QLPSTSTTSY YAPTFSTSAD FAASSVNAAS
160 170 180 190 200
DVSTASVPID TSANSIPFTT TSNIETTTSA PLTSDTPLIS TSTMSAADNV
210 220 230 240 250
FSSANPISAS LTTTDSSESF DQTSTAGAIP VQSSADFSSS SEILVQSSAD
260 270 280 290 300
FSSPSSPTTT DISLSAAPLQ TSESSSFTTA SAALPVSSTD VDGSSASPVV
310 320 330 340 350
SMSAAGQIAS SSSTDNPTMS ETFSLTSTEV DGSDVSSTVS ALLSAPFLQT
360 370 380 390 400
STSNSFSIVS PSVSFVPSQS SSDVASSSTA NVVSSSFSDI PPQTSTSGSV
410 420 430 440 450
VSVAQSASAL AFQSSTEVYG ASASSTMSSL LSTTSLQSTT LDSSSLASSS
460 470 480 490 500
ASSSDLTDYG VSSTASIPLL SASEQASTSS SFSVVSPSVS FVPSQSSSDV
510 520 530 540 550
ASTSAPSVVS SSFSYTSLQA GGSSMTNPSS STIVYSSSTG SSEESAASTA
560 570 580 590 600
SATLSGSSST YMAGNLQSQP PSTSSLLSES QATSTSAVLA SSSVSTTSPY
610 620 630 640 650
TTAGGASTEA SSLISSTSAE TSQVSYSQST TALQTSSFAS SSTTEGSETS
660 670 680 690 700
SQGFSTSSVL VQMPSSISSE FSPSQTTTQM NSASSSSQYT ISSTGILSQV
710 720 730 740 750
SDTSVSYTTS SSSVSQVSDT PVSYTTSSSS VSQVSDTPVS YTTSSSSVSQ
760 770 780 790 800
VSDTPVSYTT SSSSVSQVSD TPVSYTTSSS SVSQVSDTSV PSTSSRSSVS
810 820 830 840 850
QVSDTPVPST SSRSSVSQTS SSLQPTTTSS QRFTISTHGA LSESSSVSQQ
860 870 880 890 900
ASEITSSINA TASEYHSIQT TAATQSTTLS FTDANSSSAS APLEVATSTP
910 920 930 940 950
TPSSKASSLL LTPSTSSLSQ VATNTNVQTS LTTESTTVLE PSTTNSSSTF
960 970 980 990 1000
SLVTSSDNNW WIPTELITQA PEAASTASST VGGTQTMTLP HAIAAATQVP
1010 1020 1030 1040 1050
EPEGYTLITI GFKKALNYEF VVSEPKSSAQ IFGYLPEALN TPFKNVFTNI
1060 1070 1080 1090 1100
TVLQIVPLQD DSLNYLVSVA EVYFPTAEIE ELSNLITNSS SAFYTDGMGT
1110 1120 1130 1140 1150
AKSMAAMVDS SIPLTGLLHD SNSNSGGSSD GSSSSNSNSG SSGSGSNSNS
1160 1170 1180 1190 1200
GVSSSSGNSY QDAGTLEYSS KSNSNVSTSS KSKKKIIGLV IGVVVGGCLY
1210 1220 1230 1240 1250
ILFMIFAFKY IIRRRIQSQE IIKNPEISSI SSSEFGGEKN YNNEKRMSVQ
1260 1270 1280 1290 1300
ESITQSMRIQ NWMDDSYYGH GLTNNDSTPT RHNTSSSIPK ISRPIASQNS

LGWNEV
Length:1,306
Mass (Da):133,115
Last modified:October 1, 1993 - v1
Checksum:i67D5D984D5CA4A6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77354 Genomic DNA. Translation: AAA34798.1.
Z72799 Genomic DNA. Translation: CAA96997.1.
BK006941 Genomic DNA. Translation: DAA08111.1.
PIRiS25370.
RefSeqiNP_011528.3. NM_001181143.3.

Genome annotation databases

EnsemblFungiiYGR014W; YGR014W; YGR014W.
GeneIDi852897.
KEGGisce:YGR014W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77354 Genomic DNA. Translation: AAA34798.1.
Z72799 Genomic DNA. Translation: CAA96997.1.
BK006941 Genomic DNA. Translation: DAA08111.1.
PIRiS25370.
RefSeqiNP_011528.3. NM_001181143.3.

3D structure databases

ProteinModelPortaliP32334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33257. 53 interactors.
DIPiDIP-1455N.
IntActiP32334. 12 interactors.
MINTiMINT-396603.

PTM databases

iPTMnetiP32334.

Proteomic databases

MaxQBiP32334.
PRIDEiP32334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR014W; YGR014W; YGR014W.
GeneIDi852897.
KEGGisce:YGR014W.

Organism-specific databases

EuPathDBiFungiDB:YGR014W.
SGDiS000003246. MSB2.

Phylogenomic databases

GeneTreeiENSGT00770000121599.
InParanoidiP32334.
KOiK19849.
OMAiSLGWNEV.
OrthoDBiEOG092C480G.

Enzyme and pathway databases

BioCyciYEAST:G3O-30741-MONOMER.

Miscellaneous databases

PROiP32334.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiMSB2_YEAST
AccessioniPrimary (citable) accession number: P32334
Secondary accession number(s): D6VUF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1320 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.