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Protein

TATA-binding protein-associated factor MOT1

Gene

MOT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box via its C-terminal ATPase activity. Both transcription activation and repression require its ATPase activity.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1297 – 13048ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: SGD
  • helicase activity Source: UniProtKB-KW
  • TBP-class protein binding Source: SGD

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: SGD
  • regulation of RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • rRNA processing Source: SGD
  • transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33988-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-binding protein-associated factor MOT1 (EC:3.6.4.-)
Short name:
TBP-associated factor MOT1
Alternative name(s):
Modifier of transcription 1
Gene namesi
Name:MOT1
Ordered Locus Names:YPL082C
ORF Names:LPF4C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL082C.
SGDiS000006003. MOT1.

Subcellular locationi

  • Mitochondrion
  • Nucleus

  • Note: Localized on chromatin. Specifically localized to the promoters of the genes it regulates.

GO - Cellular componenti

  • mitochondrion Source: UniProtKB-SubCell
  • nuclear chromosome Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1226 – 12261E → R: Temperature sensitive in mot1-301. 1 Publication
Mutagenesisi1303 – 13031K → A: No ATPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18671867TATA-binding protein-associated factor MOT1PRO_0000074335Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei93 – 931PhosphoserineCombined sources
Modified residuei677 – 6771PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32333.
PRIDEiP32333.

PTM databases

iPTMnetiP32333.

Interactioni

Subunit structurei

Forms a complex with TBP which binds TATA DNA with high affinity but with altered specificity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SPT15P133939EBI-11152,EBI-19129

GO - Molecular functioni

  • TBP-class protein binding Source: SGD

Protein-protein interaction databases

BioGridi36099. 143 interactions.
DIPiDIP-2418N.
IntActiP32333. 65 interactions.
MINTiMINT-623118.

Structurei

3D structure databases

ProteinModelPortaliP32333.
SMRiP32333. Positions 241-608, 1113-1141, 1265-1813.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati289 – 32638HEAT 1Add
BLAST
Repeati445 – 48238HEAT 2Add
BLAST
Repeati541 – 57838HEAT 3Add
BLAST
Repeati1108 – 114538HEAT 4Add
BLAST
Repeati1188 – 122538HEAT 5Add
BLAST
Domaini1284 – 1457174Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Repeati1495 – 153743HEAT 6Add
BLAST
Domaini1639 – 1787149Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi195 – 21117Nuclear localization signalSequence analysisAdd
BLAST
Motifi1408 – 14114DEGH box

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 6 HEAT repeats.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00630000089754.
HOGENOMiHOG000210415.
InParanoidiP32333.
KOiK15192.
OMAiDHYYRQQ.
OrthoDBiEOG092C02J3.

Family and domain databases

Gene3Di1.25.10.10. 4 hits.
3.40.50.300. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022707. DUF3535.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF12054. DUF3535. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSRVSRLDR QVILIETGST QVVRNMAADQ MGDLAKQHPE DILSLLSRVY
60 70 80 90 100
PFLLVKKWET RVTAARAVGG IVAHAPSWDP NESDLVGGTN EGSPLDNAQV
110 120 130 140 150
KLEHEMKIKL EEATQNNQLN LLQEDHHLSS LSDWKLNEIL KSGKVLLASS
160 170 180 190 200
MNDYNVLGKA DDNIRKQAKT DDIKQETSML NASDKANENK SNANKKSARM
210 220 230 240 250
LAMARRKKKM SAKNTPKHPV DITESSVSKT LLNGKNMTNS AASLATSPTS
260 270 280 290 300
NQLNPKLEIT EQADESKLMI ESTVRPLLEQ HEIVAGLVWQ FQGIYELLLD
310 320 330 340 350
NLMSENWEIR HGAALGLREL VKKHAYGVSR VKGNTREENN LRNSRSLEDL
360 370 380 390 400
ASRLLTVFAL DRFGDYVYDT VVAPVRESVA QTLAALLIHL DSTLSIKIFN
410 420 430 440 450
CLEQLVLQDP LQTGLPNKIW EATHGGLLGI RYFVSIKTNF LFAHGLLENV
460 470 480 490 500
VRIVLYGLNQ SDDDVQSVAA SILTPITSEF VKLNNSTIEI LVTTIWSLLA
510 520 530 540 550
RLDDDISSSV GSIMDLLAKL CDHQEVLDIL KNKALEHPSE WSFKSLVPKL
560 570 580 590 600
YPFLRHSISS VRRAVLNLLI AFLSIKDDST KNWLNGKVFR LVFQNILLEQ
610 620 630 640 650
NPELLQLSFD VYVALLEHYK VKHTEKTLDH VFSKHLQPIL HLLNTPVGEK
660 670 680 690 700
GKNYAMESQY ILKPSQHYQL HPEKKRSISE TTTDSDIPIP KNNEHINIDA
710 720 730 740 750
PMIAGDITLL GLDVILNTRI MGAKAFALTL SMFQDSTLQS FFTNVLVRCL
760 770 780 790 800
ELPFSTPRML AGIIVSQFCS SWLQKHPEGE KLPSFVSEIF SPVMNKQLLN
810 820 830 840 850
RDEFPVFREL VPSLKALRTQ CQSLLATFVD VGMLPQYKLP NVAIVVQGET
860 870 880 890 900
EAGPHAFGVE TAEKVYGEYY DKMFKSMNNS YKLLAKKPLE DSKHRVLMAI
910 920 930 940 950
NSAKESAKLR TGSILANYAS SILLFDGLPL KLNPIIRSLM DSVKEERNEK
960 970 980 990 1000
LQTMAGESVV HLIQQLLENN KVNVSGKIVK NLCGFLCVDT SEVPDFSVNA
1010 1020 1030 1040 1050
EYKEKILTLI KESNSIAAQD DINLAKMSEE AQLKRKGGLI TLKILFEVLG
1060 1070 1080 1090 1100
PSILQKLPQL RSILFDSLSD HENEEASKVD NEQGQKIVDS FGVLRALFPF
1110 1120 1130 1140 1150
MSDSLRSSEV FTRFPVLLTF LRSNLSVFRY SAARTFADLA KISSVEVMAY
1160 1170 1180 1190 1200
TIREILPLMN SAGSLSDRQG STELIYHLSL SMETDVLPYV IFLIVPLLGR
1210 1220 1230 1240 1250
MSDSNEDVRN LATTTFASII KLVPLEAGIA DPKGLPEELV ASRERERDFI
1260 1270 1280 1290 1300
QQMMDPSKAK PFKLPIAIKA TLRKYQQDGV NWLAFLNKYH LHGILCDDMG
1310 1320 1330 1340 1350
LGKTLQTICI IASDQYLRKE DYEKTRSVES RALPSLIICP PSLTGHWENE
1360 1370 1380 1390 1400
FDQYAPFLKV VVYAGGPTVR LTLRPQLSDA DIIVTSYDVA RNDLAVLNKT
1410 1420 1430 1440 1450
EYNYCVLDEG HIIKNSQSKL AKAVKEITAN HRLILTGTPI QNNVLELWSL
1460 1470 1480 1490 1500
FDFLMPGFLG TEKMFQERFA KPIAASRNSK TSSKEQEAGV LALEALHKQV
1510 1520 1530 1540 1550
LPFMLRRLKE DVLSDLPPKI IQDYYCELGD LQKQLYMDFT KKQKNVVEKD
1560 1570 1580 1590 1600
IENSEIADGK QHIFQALQYM RKLCNHPALV LSPNHPQLAQ VQDYLKQTGL
1610 1620 1630 1640 1650
DLHDIINAPK LSALRTLLFE CGIGEEDIDK KASQDQNFPI QNVISQHRAL
1660 1670 1680 1690 1700
IFCQLKDMLD MVENDLFKKY MPSVTYMRLD GSIDPRDRQK VVRKFNEDPS
1710 1720 1730 1740 1750
IDCLLLTTKV GGLGLNLTGA DTVIFVEHDW NPMNDLQAMD RAHRIGQKKV
1760 1770 1780 1790 1800
VNVYRIITKG TLEEKIMGLQ KFKMNIASTV VNQQNSGLAS MDTHQLLDLF
1810 1820 1830 1840 1850
DPDNVTSQDN EEKNNGDSQA AKGMEDIANE TGLTGKAKEA LGELKELWDP
1860
SQYEEEYNLD TFIKTLR
Length:1,867
Mass (Da):209,977
Last modified:October 1, 1993 - v1
Checksum:i1A00005148D5632B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83224 Genomic DNA. Translation: AAA34786.1.
U41849 Genomic DNA. Translation: AAB68257.1.
BK006949 Genomic DNA. Translation: DAA11351.1.
PIRiS22775.
RefSeqiNP_015243.1. NM_001183896.1.

Genome annotation databases

EnsemblFungiiYPL082C; YPL082C; YPL082C.
GeneIDi856023.
KEGGisce:YPL082C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83224 Genomic DNA. Translation: AAA34786.1.
U41849 Genomic DNA. Translation: AAB68257.1.
BK006949 Genomic DNA. Translation: DAA11351.1.
PIRiS22775.
RefSeqiNP_015243.1. NM_001183896.1.

3D structure databases

ProteinModelPortaliP32333.
SMRiP32333. Positions 241-608, 1113-1141, 1265-1813.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36099. 143 interactions.
DIPiDIP-2418N.
IntActiP32333. 65 interactions.
MINTiMINT-623118.

PTM databases

iPTMnetiP32333.

Proteomic databases

MaxQBiP32333.
PRIDEiP32333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL082C; YPL082C; YPL082C.
GeneIDi856023.
KEGGisce:YPL082C.

Organism-specific databases

EuPathDBiFungiDB:YPL082C.
SGDiS000006003. MOT1.

Phylogenomic databases

GeneTreeiENSGT00630000089754.
HOGENOMiHOG000210415.
InParanoidiP32333.
KOiK15192.
OMAiDHYYRQQ.
OrthoDBiEOG092C02J3.

Enzyme and pathway databases

BioCyciYEAST:G3O-33988-MONOMER.

Miscellaneous databases

PROiP32333.

Family and domain databases

Gene3Di1.25.10.10. 4 hits.
3.40.50.300. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR022707. DUF3535.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF12054. DUF3535. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOT1_YEAST
AccessioniPrimary (citable) accession number: P32333
Secondary accession number(s): D6W3T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6560 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.