ID   DGR2_YEAST              Reviewed;         852 AA.
AC   P32330; D6VXG9;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   27-MAR-2024, entry version 165.
DE   RecName: Full=2-deoxy-glucose resistant protein 2;
GN   Name=DGR2; OrderedLocusNames=YKL121W; ORFNames=YKL525;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1514329; DOI=10.1002/yea.320080410;
RA   Colleaux L., Richard G.-F., Thierry A., Dujon B.;
RT   "Sequence of a segment of yeast chromosome XI identifies a new
RT   mitochondrial carrier, a new member of the G protein family, and a protein
RT   with the PAAKK motif of the H1 histones.";
RL   Yeast 8:325-336(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8196765; DOI=10.1038/369371a0;
RA   Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA   Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA   Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA   Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA   Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA   Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA   Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA   Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA   Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA   Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA   Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA   Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA   Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA   Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA   Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA   Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA   Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA   Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA   Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA   van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA   von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA   Becker I., Mewes H.-W.;
RT   "Complete DNA sequence of yeast chromosome XI.";
RL   Nature 369:371-378(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [6]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=19004802; DOI=10.1073/pnas.0803090105;
RA   Ralser M., Wamelink M.M., Struys E.A., Joppich C., Krobitsch S., Jakobs C.,
RA   Lehrach H.;
RT   "A catabolic block does not sufficiently explain how 2-deoxy-D-glucose
RT   inhibits cell growth.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:17807-17811(2008).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- DISRUPTION PHENOTYPE: Increases cellular tolerance to 2-deoxy-glucose.
CC       {ECO:0000269|PubMed:19004802}.
CC   -!- MISCELLANEOUS: Present with 504 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the WD repeat DGR2 family. {ECO:0000305}.
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DR   EMBL; S44213; AAB23072.2; -; Genomic_DNA.
DR   EMBL; Z28121; CAA81962.1; -; Genomic_DNA.
DR   EMBL; BK006944; DAA09039.1; -; Genomic_DNA.
DR   PIR; S25359; S25359.
DR   RefSeq; NP_012801.1; NM_001179687.1.
DR   AlphaFoldDB; P32330; -.
DR   BioGRID; 34014; 139.
DR   IntAct; P32330; 1.
DR   STRING; 4932.YKL121W; -.
DR   iPTMnet; P32330; -.
DR   MaxQB; P32330; -.
DR   PaxDb; 4932-YKL121W; -.
DR   PeptideAtlas; P32330; -.
DR   EnsemblFungi; YKL121W_mRNA; YKL121W; YKL121W.
DR   GeneID; 853738; -.
DR   KEGG; sce:YKL121W; -.
DR   AGR; SGD:S000001604; -.
DR   SGD; S000001604; DGR2.
DR   VEuPathDB; FungiDB:YKL121W; -.
DR   eggNOG; KOG0283; Eukaryota.
DR   GeneTree; ENSGT00940000157557; -.
DR   HOGENOM; CLU_008245_0_0_1; -.
DR   InParanoid; P32330; -.
DR   OMA; WKVINSP; -.
DR   OrthoDB; 11918at2759; -.
DR   BioCyc; YEAST:G3O-31904-MONOMER; -.
DR   BioGRID-ORCS; 853738; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P32330; -.
DR   Proteomes; UP000002311; Chromosome XI.
DR   RNAct; P32330; Protein.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   InterPro; IPR040324; WDR44/Dgr2.
DR   PANTHER; PTHR14221; WD REPEAT DOMAIN 44; 1.
DR   PANTHER; PTHR14221:SF0; WD REPEAT-CONTAINING PROTEIN 44; 1.
DR   Pfam; PF00400; WD40; 3.
DR   SMART; SM00320; WD40; 4.
DR   SUPFAM; SSF50978; WD40 repeat-like; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 1.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..852
FT                   /note="2-deoxy-glucose resistant protein 2"
FT                   /id="PRO_0000051479"
FT   REPEAT          171..210
FT                   /note="WD 1"
FT   REPEAT          278..316
FT                   /note="WD 2"
FT   REPEAT          318..358
FT                   /note="WD 3"
FT   REPEAT          426..471
FT                   /note="WD 4"
FT   REPEAT          476..515
FT                   /note="WD 5"
FT   REPEAT          651..689
FT                   /note="WD 6"
FT   REGION          1..60
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          723..748
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..35
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        36..54
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        731..746
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         716
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
SQ   SEQUENCE   852 AA;  96022 MW;  A673BAF9AF22B608 CRC64;
     MFKSKTSTLS YDETPNSNEG DRNATPVNPK EKSQTKHLNI PGDRSRHSSI ADSKRSSSRY
     DGGYSADIIP AQLRFIDNID YGTRLRKTLH RNSVVSNGYN KLSENDRWYF DLFDRKYFEN
     YLEEPTYIKI FKKKEGLEQF DRMFLAQELK IPDVYKSTTY QGEPAVANSE LFKNSICCCT
     FSHDGKYMVI GCKDGSLHLW KVINSPVKRS EMGRSEKSVS ASRANSLKIQ RHLASISSHN
     GSISSNDLKP SDQFEGPSKQ LHLYAPVFYS DVFRVFMEHA LDILDANWSK NGFLITASMD
     KTAKLWHPER KYSLKTFVHP DFVTSAIFFP NDDRFIITGC LDHRCRLWSI LDNEVSYAFD
     CKDLITSLTL SPPGGEYTII GTFNGYIYVL LTHGLKFVSS FHVSDKSTQG TTKNSFHPSS
     EYGKVQHGPR ITGLQCFFSK VDKNLRLIVT TNDSKIQIFD LNEKKPLELF KGFQSGSSRH
     RGQFLMMKNE PVVFTGSDDH WFYTWKMQSF NLSAEMNCTA PHRKKRLSGS MSLKGLLRIV
     SNKSTNDECL TETSNQSSSH TFTNSSKNVL QTQTVGSQAI KNNHYISFHA HNSPVTCASI
     APDVAIKNLS LSNDLIFELT SQYFKEMGQN YSESKETCDN KPNHPVTETG GFSSNLSNVV
     NNVGTILITT DSQGLIRVFR TDILPEIRKK IIEKFHEYNL FHLEAAGKIN NHNNDSILEN
     RMDERSSTED NEFSTTPPSN THNSRPSHDF CELHPNNSPV ISGMPSRASA IFKNSIFNKS
     NGSFISLKSR SESTSSTVFG PHDIPRVSTT YPKLKCDVCN GSNFECASKN PIAGGDSGFT
     CADCGTILNN FR
//