P32330 (DGR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-deoxy-glucose resistant protein 2

Gene names

Name:	DGR2
Ordered Locus Names:	YKL121W
ORF Names:	YKL525

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	852 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Disruption phenotype	Increases cellular tolerance to 2-deoxy-glucose. Ref.6
Miscellaneous	Present with 504 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the WD repeat DGR2 family. Contains 6 WD repeats.

Ontologies

Keywords
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 852	852	2-deoxy-glucose resistant protein 2		PRO_0000051479
Regions
	Repeat	171 – 210	40	WD 1
	Repeat	278 – 316	39	WD 2
	Repeat	318 – 358	41	WD 3
	Repeat	426 – 471	46	WD 4
	Repeat	476 – 515	40	WD 5
	Repeat	651 – 689	39	WD 6
Amino acid modifications
	Modified residue	10	1	Phosphoserine Ref.5
	Modified residue	96	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P32330 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: A673BAF9AF22B608	FASTA	852	96,022
	10 20 30 40 50 60 MFKSKTSTLS YDETPNSNEG DRNATPVNPK EKSQTKHLNI PGDRSRHSSI ADSKRSSSRY 70 80 90 100 110 120 DGGYSADIIP AQLRFIDNID YGTRLRKTLH RNSVVSNGYN KLSENDRWYF DLFDRKYFEN 130 140 150 160 170 180 YLEEPTYIKI FKKKEGLEQF DRMFLAQELK IPDVYKSTTY QGEPAVANSE LFKNSICCCT 190 200 210 220 230 240 FSHDGKYMVI GCKDGSLHLW KVINSPVKRS EMGRSEKSVS ASRANSLKIQ RHLASISSHN 250 260 270 280 290 300 GSISSNDLKP SDQFEGPSKQ LHLYAPVFYS DVFRVFMEHA LDILDANWSK NGFLITASMD 310 320 330 340 350 360 KTAKLWHPER KYSLKTFVHP DFVTSAIFFP NDDRFIITGC LDHRCRLWSI LDNEVSYAFD 370 380 390 400 410 420 CKDLITSLTL SPPGGEYTII GTFNGYIYVL LTHGLKFVSS FHVSDKSTQG TTKNSFHPSS 430 440 450 460 470 480 EYGKVQHGPR ITGLQCFFSK VDKNLRLIVT TNDSKIQIFD LNEKKPLELF KGFQSGSSRH 490 500 510 520 530 540 RGQFLMMKNE PVVFTGSDDH WFYTWKMQSF NLSAEMNCTA PHRKKRLSGS MSLKGLLRIV 550 560 570 580 590 600 SNKSTNDECL TETSNQSSSH TFTNSSKNVL QTQTVGSQAI KNNHYISFHA HNSPVTCASI 610 620 630 640 650 660 APDVAIKNLS LSNDLIFELT SQYFKEMGQN YSESKETCDN KPNHPVTETG GFSSNLSNVV 670 680 690 700 710 720 NNVGTILITT DSQGLIRVFR TDILPEIRKK IIEKFHEYNL FHLEAAGKIN NHNNDSILEN 730 740 750 760 770 780 RMDERSSTED NEFSTTPPSN THNSRPSHDF CELHPNNSPV ISGMPSRASA IFKNSIFNKS 790 800 810 820 830 840 NGSFISLKSR SESTSSTVFG PHDIPRVSTT YPKLKCDVCN GSNFECASKN PIAGGDSGFT 850 CADCGTILNN FR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a segment of yeast chromosome XI identifies a new mitochondrial carrier, a new member of the G protein family, and a protein with the PAAKK motif of the H1 histones." Colleaux L., Richard G.-F., Thierry A., Dujon B. Yeast 8:325-336(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10 AND SER-96, MASS SPECTROMETRY.
[6]	"A catabolic block does not sufficiently explain how 2-deoxy-D-glucose inhibits cell growth." Ralser M., Wamelink M.M., Struys E.A., Joppich C., Krobitsch S., Jakobs C., Lehrach H. Proc. Natl. Acad. Sci. U.S.A. 105:17807-17811(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S44213 Genomic DNA. Translation: AAB23072.2. Z28121 Genomic DNA. Translation: CAA81962.1. BK006944 Genomic DNA. Translation: DAA09039.1.
PIR	S25359.
RefSeq	NP_012801.1. NM_001179687.1.
3D structure databases
ProteinModelPortal	P32330.
SMR	P32330. Positions 171-509.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32330. 1 interaction.
STRING	4932.YKL121W.
Proteomic databases
PaxDb	P32330.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL121W; YKL121W; YKL121W.
GeneID	853738.
KEGG	sce:YKL121W.
Organism-specific databases
CYGD	YKL121w.
SGD	S000001604. DGR2.
Phylogenomic databases
eggNOG	NOG270909.
GeneTree	ENSGT00530000063479.
OMA	KTFVHPD.
OrthoDB	EOG46DQBD.
Gene expression databases
Genevestigator	P32330.
GermOnline	YKL121W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 4 hits.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 4 hits. [Graphical view]
SMART	SM00320. WD40. 4 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 1 hit. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974787.

Entry information

Entry name

DGR2_YEAST

Accession

Primary (citable) accession number: P32330
Secondary accession number(s): D6VXG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program