P32323 (AGA1_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 86.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: A-agglutinin anchorage subunit Alternative name(s): A-agglutinin cell wall attachment subunit | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › ![]() |
Protein attributes
| Sequence length | 725 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Cell wall anchoring subunit of the a-agglutinin heterodimer. S.cerevisiae a and alpha cells express the complementary cell surface glycoproteins a-agglutinin and alpha-agglutinin, respectively, which interact with one another to promote cellular aggregation during mating. |
| Subunit structure | |
| Subcellular location | Secreted › cell wall. Membrane; Lipid-anchor › GPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP). Ref.6 |
| Domain | The number of the intragenic tandem repeats varies between different S.cerevisiae strains. |
| Post-translational modification | Extensively O-glycosylated by PMT1 and PMT2. Ref.6 The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell adhesion Pheromone response |
| Cellular component | Cell wall Membrane Secreted |
| Domain | Repeat Signal |
| PTM | Disulfide bond GPI-anchor Glycoprotein Lipoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | agglutination involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.1Ref.4. Source: SGD |
| Cellular_component | anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular regionInferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wallInferred from direct assay Ref.1Ref.4. Source: SGD |
| Molecular_function | cell adhesion molecule binding Inferred from mutant phenotype Ref.1Ref.4. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 24 | 24 | Potential | ||||||
| Chain | 25 – 699 | 675 | A-agglutinin anchorage subunit | PRO_0000020641 | |||||
| Propeptide | 700 – 725 | 26 | Removed in mature form Potential | PRO_0000296627 | |||||
Regions | |||||||||
| Repeat | 53 – 149 | 97 | 1-1 | ||||||
| Repeat | 182 – 188 | 7 | 2-1 | ||||||
| Repeat | 189 – 195 | 7 | 2-2 | ||||||
| Repeat | 196 – 202 | 7 | 2-3 | ||||||
| Repeat | 203 – 209 | 7 | 2-4 | ||||||
| Repeat | 210 – 216 | 7 | 2-5 | ||||||
| Repeat | 217 – 223 | 7 | 2-6 | ||||||
| Repeat | 224 – 230 | 7 | 2-7 | ||||||
| Repeat | 231 – 237 | 7 | 2-8 | ||||||
| Repeat | 238 – 244 | 7 | 2-9 | ||||||
| Repeat | 245 – 251 | 7 | 2-10 | ||||||
| Repeat | 252 – 258 | 7 | 2-11 | ||||||
| Repeat | 259 – 265 | 7 | 2-12 | ||||||
| Repeat | 266 – 272 | 7 | 2-13 | ||||||
| Repeat | 273 – 279 | 7 | 2-14 | ||||||
| Repeat | 280 – 286 | 7 | 2-15 | ||||||
| Repeat | 287 – 293 | 7 | 2-16 | ||||||
| Repeat | 294 – 300 | 7 | 2-17 | ||||||
| Repeat | 301 – 307 | 7 | 2-18 | ||||||
| Repeat | 395 – 493 | 99 | 1-2 | ||||||
| Region | 53 – 493 | 441 | 2 X approximate repeats | ||||||
| Region | 182 – 307 | 126 | 18 X approximate tandem repeats, Ser/Thr-rich | ||||||
Amino acid modifications | |||||||||
| Lipidation | 699 | 1 | GPI-anchor amidated glycine Potential | ||||||
| Disulfide bond | 133 | Interchain (with AGA2) Probable | |||||||
| Disulfide bond | 136 | Interchain (with AGA2) Probable | |||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The AGA1 product is involved in cell surface attachment of the Saccharomyces cerevisiae cell adhesion glycoprotein a-agglutinin." Roy A., Lu C.F., Marykwas D.L., Lipke P.N., Kurjan J. Mol. Cell. Biol. 11:4196-4206(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J.Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [4] | "Mating type-specific cell-cell recognition of Saccharomyces cerevisiae: cell wall attachment and active sites of a- and alpha-agglutinin." Cappellaro C., Baldermann C., Rachel R., Tanner W. EMBO J. 13:4737-4744(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT. |
| [5] | "Delineation of functional regions within the subunits of the Saccharomyces cerevisiae cell adhesion molecule a-agglutinin." Shen Z.M., Wang L., Pike J., Jue C.K., Zhao H., de Nobel H., Kurjan J., Lipke P.N. J. Biol. Chem. 276:15768-15775(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, DISULFIDE BONDS. |
| [6] | "Posttranslational modifications required for cell surface localization and function of the fungal adhesin Aga1p." Huang G., Zhang M., Erdman S.E. Eukaryot. Cell 2:1099-1114(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR, GLYCOSYLATION, SUBCELLULAR LOCATION. |
| [7] | "Intragenic tandem repeats generate functional variability." Verstrepen K.J., Jansen A., Lewitter F., Fink G.R. Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract] Cited for: REPEATS. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M60590 Genomic DNA. Translation: AAA34382.1. Z71659 Genomic DNA. Translation: CAA96325.1. BK006947 Genomic DNA. Translation: DAA10585.1. |
| PIR | A41258. |
| RefSeq | NP_014442.1. NM_001183221.1. |
3D structure databases | |
| ProteinModelPortal | P32323. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P32323. 2 interactions. |
| STRING | 4932.YNR044W. |
Proteomic databases | |
| PaxDb | P32323. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YNR044W; YNR044W; YNR044W. |
| GeneID | 855780. |
| KEGG | sce:YNR044W. |
Organism-specific databases | |
| CYGD | YNR044w. |
| SGD | S000005327. AGA1. |
Phylogenomic databases | |
| eggNOG | NOG12793. |
| GeneTree | ENSGT00700000104630. |
| OMA | TETECNK. |
Gene expression databases | |
| Genevestigator | P32323. |
| GermOnline | YNR044W. Saccharomyces cerevisiae. |
Family and domain databases | |
| ProtoNet | Search... |
Other | |
| NextBio | 980247. |
Entry information
| Entry name | AGA1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P32323 Secondary accession number(s): D6W1L9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIV Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names |

Clusters with
