P32323 (AGA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A-agglutinin anchorage subunit

Alternative name(s):
A-agglutinin cell wall attachment subunit

Gene names

Name:	AGA1
Ordered Locus Names:	YNR044W
ORF Names:	N3431

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell wall anchoring subunit of the a-agglutinin heterodimer. S.cerevisiae a and alpha cells express the complementary cell surface glycoproteins a-agglutinin and alpha-agglutinin, respectively, which interact with one another to promote cellular aggregation during mating.
Subunit structure	Heterodimer; disulfide-linked Probable. Ref.4 Ref.5
Subcellular location	Secreted › cell wall. Membrane; Lipid-anchor › GPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP). Ref.6
Domain	The number of the intragenic tandem repeats varies between different S.cerevisiae strains.
Post-translational modification	Extensively O-glycosylated by PMT1 and PMT2. Ref.6 The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Ontologies

Keywords
Biological process	Cell adhesion Pheromone response
Cellular component	Cell wall Membrane Secreted
Domain	Repeat Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	agglutination involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.1Ref.4. Source: SGD
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall Inferred from direct assay Ref.1Ref.4. Source: SGD
Molecular_function	cell adhesion molecule binding Inferred from mutant phenotype Ref.1Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 699	675	A-agglutinin anchorage subunit		PRO_0000020641
	Propeptide	700 – 725	26	Removed in mature form Potential		PRO_0000296627
Regions
	Repeat	53 – 149	97	1-1
	Repeat	182 – 188	7	2-1
	Repeat	189 – 195	7	2-2
	Repeat	196 – 202	7	2-3
	Repeat	203 – 209	7	2-4
	Repeat	210 – 216	7	2-5
	Repeat	217 – 223	7	2-6
	Repeat	224 – 230	7	2-7
	Repeat	231 – 237	7	2-8
	Repeat	238 – 244	7	2-9
	Repeat	245 – 251	7	2-10
	Repeat	252 – 258	7	2-11
	Repeat	259 – 265	7	2-12
	Repeat	266 – 272	7	2-13
	Repeat	273 – 279	7	2-14
	Repeat	280 – 286	7	2-15
	Repeat	287 – 293	7	2-16
	Repeat	294 – 300	7	2-17
	Repeat	301 – 307	7	2-18
	Repeat	395 – 493	99	1-2
	Region	53 – 493	441	2 X approximate repeats
	Region	182 – 307	126	18 X approximate tandem repeats, Ser/Thr-rich
Amino acid modifications
	Lipidation	699	1	GPI-anchor amidated glycine Potential
	Disulfide bond	133		Interchain (with AGA2) Probable
	Disulfide bond	136		Interchain (with AGA2) Probable

Sequences

	Sequence		Length	Mass (Da)
	P32323 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 70420C853B0B01F8	FASTA	725	73,354
	10 20 30 40 50 60 MTLSFAHFTY LFTILLGLTN IALASDPETI LVTITKTNDA NGVVTTTVSP ALVSTSTIVQ 70 80 90 100 110 120 AGTTTLYTTW CPLTVSTSSA AEISPSISYA TTLSRFSTLT LSTEVCSHEA CPSSSTLPTT 130 140 150 160 170 180 TLSVTSKFTS YICPTCHTTA ISSLSEVGTT TVVSSSAIEP SSASIISPVT STLSSTTSSN 190 200 210 220 230 240 PTTTSLSSTS TSPSSTSTSP SSTSTSSSST STSSSSTSTS SSSTSTSPSS TSTSSSLTST 250 260 270 280 290 300 SSSSTSTSQS STSTSSSSTS TSPSSTSTSS SSTSTSPSSK STSASSTSTS SYSTSTSPSL 310 320 330 340 350 360 TSSSPTLAST SPSSTSISST FTDSTSSLGS SIASSSTSVS LYSPSTPVYS VPSTSSNVAT 370 380 390 400 410 420 PSMTSSTVET TVSSQSSSEY ITKSSISTTI PSFSMSTYFT TVSGVTTMYT TWCPYSSESE 430 440 450 460 470 480 TSTLTSMHET VTTDATVCTH ESCMPSQTTS LITSSIKMST KNVATSVSTS TVESSYACST 490 500 510 520 530 540 CAETSHSYSS VQTASSSSVT QQTTSTKSWV SSMTTSDEDF NKHATGKYHV TSSGTSTIST 550 560 570 580 590 600 SVSEATSTSS IDSESQEQSS HLLSTSVLSS SSLSATLSSD STILLFSSVS SLSVEQSPVT 610 620 630 640 650 660 TLQISSTSEI LQPTSSTAIA TISASTSSLS ATSISTPSTS VESTIESSSL TPTVSSIFLS 670 680 690 700 710 720 SSSAPSSLQT SVTTTEVSTT SISIQYQTSS MVTISQYMGS GSQTRLPLGK LVFAIMAVAC NVIFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The AGA1 product is involved in cell surface attachment of the Saccharomyces cerevisiae cell adhesion glycoprotein a-agglutinin." Roy A., Lu C.F., Marykwas D.L., Lipke P.N., Kurjan J. Mol. Cell. Biol. 11:4196-4206(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Mating type-specific cell-cell recognition of Saccharomyces cerevisiae: cell wall attachment and active sites of a- and alpha-agglutinin." Cappellaro C., Baldermann C., Rachel R., Tanner W. EMBO J. 13:4737-4744(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[5]	"Delineation of functional regions within the subunits of the Saccharomyces cerevisiae cell adhesion molecule a-agglutinin." Shen Z.M., Wang L., Pike J., Jue C.K., Zhao H., de Nobel H., Kurjan J., Lipke P.N. J. Biol. Chem. 276:15768-15775(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, DISULFIDE BONDS.
[6]	"Posttranslational modifications required for cell surface localization and function of the fungal adhesin Aga1p." Huang G., Zhang M., Erdman S.E. Eukaryot. Cell 2:1099-1114(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GPI-ANCHOR, GLYCOSYLATION, SUBCELLULAR LOCATION.
[7]	"Intragenic tandem repeats generate functional variability." Verstrepen K.J., Jansen A., Lewitter F., Fink G.R. Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract] Cited for: REPEATS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60590 Genomic DNA. Translation: AAA34382.1. Z71659 Genomic DNA. Translation: CAA96325.1. BK006947 Genomic DNA. Translation: DAA10585.1.
PIR	A41258.
RefSeq	NP_014442.1. NM_001183221.1.
3D structure databases
ProteinModelPortal	P32323.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32323. 2 interactions.
STRING	4932.YNR044W.
Proteomic databases
PaxDb	P32323.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR044W; YNR044W; YNR044W.
GeneID	855780.
KEGG	sce:YNR044W.
Organism-specific databases
CYGD	YNR044w.
SGD	S000005327. AGA1.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00700000104630.
OMA	TETECNK.
Gene expression databases
Genevestigator	P32323.
GermOnline	YNR044W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	980247.

Entry information

Entry name

AGA1_YEAST

Accession

Primary (citable) accession number: P32323
Secondary accession number(s): D6W1L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program