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P32321 (DCTD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxycytidylate deaminase

EC=3.5.4.12
Alternative name(s):
dCMP deaminase
Gene names
Name:DCTD
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length178 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Supplies the nucleotide substrate for thymidylate synthetase.

Catalytic activity

dCMP + H2O = dUMP + NH3.

Cofactor

Zinc.

Enzyme regulation

Allosteric enzyme whose activity is greatly influenced by the end products of its metabolic pathway, dCTP and dTTP.

Subunit structure

Homohexamer.

Sequence similarities

Belongs to the cytidine and deoxycytidylate deaminase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P32321-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P32321-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVGGGQPCGPNM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 178178Deoxycytidylate deaminase
PRO_0000171691

Sites

Active site861Proton donor By similarity
Metal binding841Zinc; catalytic
Metal binding1101Zinc; catalytic
Metal binding1131Zinc; catalytic

Natural variations

Alternative sequence11M → MVGGGQPCGPNM in isoform 2.
VSP_038094

Experimental info

Sequence conflict951S → L in AAH01286. Ref.6
Sequence conflict1281M → T in AAA35755. Ref.1

Secondary structure

.............................. 178
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 19, 2002. Version 2.
Checksum: 2B8DA5EAC85F3666

FASTA17820,016
        10         20         30         40         50         60 
MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC 

        70         80         90        100        110        120 
SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA 

       130        140        150        160        170 
GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ 

« Hide

Isoform 2 [UniParc].

Checksum: 973677A3294EBB97
Show »

FASTA18921,014

References

« Hide 'large scale' references
[1]"Primary structure of human deoxycytidylate deaminase and overexpression of its functional protein in Escherichia coli."
Weiner K.X., Weiner R.S., Maley F., Maley G.F.
J. Biol. Chem. 268:12983-12989(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
[2]"Chromosomal location and structural organization of the human deoxycytidylate deaminase gene."
Weiner K.X., Ciesla J., Jaffe A.B., Ketring R., Maley F., Maley G.F.
J. Biol. Chem. 270:18727-18729(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Lung.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Cervix and Testis.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"The crystal structure of human dCMP deaminase."
Structural genomics consortium (SGC)
Submitted (NOV-2008) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 5-173 IN COMPLEX WITH ZINC.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12136 mRNA. Translation: AAA35755.1.
L39874 Genomic DNA. Translation: AAC37579.1.
AK313221 mRNA. Translation: BAG36033.1.
AC079766 Genomic DNA. No translation available.
CH471056 Genomic DNA. Translation: EAX04697.1.
CH471056 Genomic DNA. Translation: EAX04698.1.
CH471056 Genomic DNA. Translation: EAX04700.1.
CH471056 Genomic DNA. Translation: EAX04701.1.
BC001286 mRNA. Translation: AAH01286.1.
BC088357 mRNA. Translation: AAH88357.2.
PIRA47288.
I55434.
RefSeqNP_001012750.1. NM_001012732.1.
NP_001912.2. NM_001921.2.
XP_005262836.1. XM_005262779.1.
XP_005262837.1. XM_005262780.1.
UniGeneHs.183850.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2W4LX-ray2.10A/B/C/D/E/F5-173[»]
ProteinModelPortalP32321.
SMRP32321. Positions 12-173.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108003. 7 interactions.
IntActP32321. 4 interactions.
MINTMINT-3013156.
STRING9606.ENSP00000349576.

Chemistry

ChEMBLCHEMBL5675.

PTM databases

PhosphoSiteP32321.

Polymorphism databases

DMDM23503055.

Proteomic databases

PaxDbP32321.
PRIDEP32321.

Protocols and materials databases

DNASU1635.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000357067; ENSP00000349576; ENSG00000129187. [P32321-2]
ENST00000438320; ENSP00000398194; ENSG00000129187. [P32321-1]
ENST00000510370; ENSP00000424017; ENSG00000129187. [P32321-1]
GeneID1635.
KEGGhsa:1635.
UCSCuc003ivf.3. human. [P32321-1]
uc003ivg.3. human. [P32321-2]

Organism-specific databases

CTD1635.
GeneCardsGC04M183811.
HGNCHGNC:2710. DCTD.
HPAHPA035894.
MIM607638. gene.
neXtProtNX_P32321.
PharmGKBPA138.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2131.
HOGENOMHOG000015715.
HOVERGENHBG025823.
InParanoidP32321.
KOK01493.
OMALMFEMAG.
OrthoDBEOG7JDQZR.
PhylomeDBP32321.
TreeFamTF105971.

Enzyme and pathway databases

BioCycMetaCyc:HS05252-MONOMER.
ReactomeREACT_111217. Metabolism.
SABIO-RKP32321.

Gene expression databases

ArrayExpressP32321.
BgeeP32321.
CleanExHS_DCTD.
GenevestigatorP32321.

Family and domain databases

InterProIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR015517. Cyt_deaminase.
IPR016193. Cytidine_deaminase-like.
IPR016473. dCMP_deaminase.
[Graphical view]
PANTHERPTHR11086. PTHR11086. 1 hit.
PfamPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
PIRSFPIRSF006019. dCMP_deaminase. 1 hit.
SUPFAMSSF53927. SSF53927. 1 hit.
PROSITEPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDCTD. human.
EvolutionaryTraceP32321.
GenomeRNAi1635.
NextBio6716.
PROP32321.
SOURCESearch...

Entry information

Entry nameDCTD_HUMAN
AccessionPrimary (citable) accession number: P32321
Secondary accession number(s): B2R836 expand/collapse secondary AC list , D3DP49, D3DP50, Q5M7Z8, Q9BVD8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 19, 2002
Last modified: April 16, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM