Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar protein sorting/targeting protein VPS10

Gene

PEP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal.7 Publications

GO - Molecular functioni

  • nucleotide binding Source: UniProtKB-KW
  • signal sequence binding Source: SGD

GO - Biological processi

  • protein targeting to vacuole Source: SGD
  • vacuolar transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28921-MONOMER.

Protein family/group databases

TCDBi9.A.63.1.1. the retromer-dependnet vacuolar protein sorting (r-vps) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting/targeting protein VPS10
Alternative name(s):
Carboxypeptidase Y receptor
Short name:
CPY receptor
Carboxypeptidase Y-deficient protein 1
Sortilin VPS10
Vacuolar carboxypeptidase sorting receptor VPS10
Vacuolar protein sorting-associated protein 10
Vacuolar protein-targeting protein 1
Gene namesi
Name:PEP1
Synonyms:VPS10, VPT1
Ordered Locus Names:YBL017C
ORF Names:YBL03.22, YBL0302
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL017C.
SGDiS000000113. PEP1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 1397LumenalSequence analysisAdd BLAST1376
Transmembranei1398 – 1414HelicalSequence analysisAdd BLAST17
Topological domaini1415 – 1579CytoplasmicSequence analysisAdd BLAST165

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • late endosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000002203722 – 1579Vacuolar protein sorting/targeting protein VPS10Add BLAST1558

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi96N-linked (GlcNAc...)Sequence analysis1
Glycosylationi170N-linked (GlcNAc...)Sequence analysis1
Glycosylationi447N-linked (GlcNAc...)Sequence analysis1
Glycosylationi793N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1010N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1303N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP32319.
PRIDEiP32319.

Interactioni

Protein-protein interaction databases

BioGridi32681. 118 interactors.
DIPiDIP-5900N.
IntActiP32319. 23 interactors.
MINTiMINT-630886.

Structurei

3D structure databases

ProteinModelPortaliP32319.
SMRiP32319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 68BNR 1Add BLAST12
Repeati101 – 112BNR 2Add BLAST12
Repeati179 – 190BNR 3Add BLAST12
Repeati415 – 426BNR 4Add BLAST12
Repeati487 – 498BNR 5Add BLAST12
Repeati533 – 544BNR 6Add BLAST12
Repeati764 – 775BNR 7Add BLAST12
Repeati861 – 872BNR 8Add BLAST12
Repeati1143 – 1154BNR 9Add BLAST12
Repeati1184 – 1195BNR 10Add BLAST12

Domaini

The lumenal domain contains two regions of approximately 650 AA that exhibit 20% identity. The cytoplasmic domain serves as a Golgi retention/recycling signal.3 Publications

Sequence similaritiesi

Belongs to the VPS10-related sortilin family.Curated
Contains 10 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046443.
InParanoidiP32319.
OrthoDBiEOG092C0D5Y.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
InterProiIPR011040. Sialidases.
IPR031777. Sortilin_C.
IPR031778. Sortilin_N.
IPR006581. VPS10.
[Graphical view]
PfamiPF15902. Sortilin-Vps10. 2 hits.
PF15901. Sortilin_C. 2 hits.
[Graphical view]
SMARTiSM00602. VPS10. 2 hits.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILLHFVYSL WALLLIPLTN AEEFTPKVTK TIAQDSFDIL SFDDSNTLIR
60 70 80 90 100
KQDTSVTISF DDGETWEKVE GIEGEITWIY IDPFNRHDRA VATAMNGSYL
110 120 130 140 150
YITNDQGKSW ERITLPDSGE SISPRECYIE THPLNKNYFL AKCNYCEKTE
160 170 180 190 200
VNNDNEENSG DEEGQFEIFN ITRCTDKVFA SNDGGKSFSE IKSSLERNEN
210 220 230 240 250
SPISISDCGF AKTSKDSDLE SSDTSIICLF QNMQLIMDEF SSPYTESKLV
260 270 280 290 300
LTTDWGKSLK EFDQFKDKVV NGYRILKSHM VVLTQGDRYN DMSSMDVWVS
310 320 330 340 350
NDLSNFKMAY MPTQLRHSMQ GEIYEDAMGR IILPMSRERS DQEEDKGIVS
360 370 380 390 400
EILISDSQGL KFSPIPWTAN EVFGYINFYQ PTYLKGTMIA SLYPLSRRRN
410 420 430 440 450
RKGKAKGVKS KGVTKISVDN GLTWTMLKVV DPDNADSFDC DITDFENCSL
460 470 480 490 500
QNMFYTREGS TPTAGILMTT GIVGDGSVFD WGDQRTFISR DGGLTWKLAF
510 520 530 540 550
DFPCLYAVGD YGNVIVAIPY NADEDDDPQS EFYYSLDQGK TWTEYQLETT
560 570 580 590 600
IYPNEVMNTT PDGSGAKFIL NGFTLAHMDG TTNFIYAIDF STAFNDKTCE
610 620 630 640 650
ENDFEDWNLA EGKCVNGVKY KIRRRKQDAQ CLVKKVFEDL QLFETACDKC
660 670 680 690 700
TEADYECAFE FVRDATGKCV PDYNLIVLSD VCDKTKKKTV PVKPLQLVKG
710 720 730 740 750
DKCKKPMTVK SVDISCEGVP KKGTNDKEIV VTENKFDFKI QFYQYFDTVT
760 770 780 790 800
DESLLMINSR GEAYISHDGG QTIKRFDSNG ETIIEVVFNP YYNSSAYLFG
810 820 830 840 850
SKGSIFSTHD RGYSFMTAKL PEARQLGMPL DFNAKAQDTF IYYGGKNCES
860 870 880 890 900
ILSPECHAVA YLTNDGGETF TEMLDNAIHC EFAGSLFKYP SNEDMVMCQV
910 920 930 940 950
KEKSSQTRSL VSSTDFFQDD KNTVFENIIG YLSTGGYIIV AVPHENNELR
960 970 980 990 1000
AYVTIDGTEF AEAKFPYDED VGKQEAFTIL ESEKGSIFLH LATNLVPGRD
1010 1020 1030 1040 1050
FGNLLKSNSN GTSFVTLEHA VNRNTFGYVD FEKIQGLEGI ILTNIVSNSD
1060 1070 1080 1090 1100
KVAENKEDKQ LKTKITFNEG SDWNFLKPPK RDSEGKKFSC SSKSLDECSL
1110 1120 1130 1140 1150
HLHGYTERKD IRDTYSSGSA LGMMFGVGNV GPNLLPYKEC STFFTTDGGE
1160 1170 1180 1190 1200
TWAEVKKTPH QWEYGDHGGI LVLVPENSET DSISYSTDFG KTWKDYKFCA
1210 1220 1230 1240 1250
DKVLVKDITT VPRDSALRFL LFGEAADIGG SSFRTYTIDF RNIFERQCDF
1260 1270 1280 1290 1300
DITGKESADY KYSPLGSKSN CLFGHQTEFL RKTDENCFIG NIPLSEFSRN
1310 1320 1330 1340 1350
IKNCSCTRQD FECDYNFYKA NDGTCKLVKG LSPANAADVC KKEPDLIEYF
1360 1370 1380 1390 1400
ESSGYRKIPL STCEGGLKLD APSSPHACPG KEKEFKEKYS VSAGPFAFIF
1410 1420 1430 1440 1450
ISILLIIFFA AWFVYDRGIR RNGGFARFGE IRLGDDGLIE NNNTDRVVNN
1460 1470 1480 1490 1500
IVKSGFYVFS NIGSLLQHTK TNIAHAISKI RGRFGNRTGP SYSSLIHDQF
1510 1520 1530 1540 1550
LDEADDLLAG HDEDANDLSS FMDQGSNFEI EEDDVPTLEE EHTSYTDQPT
1560 1570
TTDVPDTLPE GNEENIDRPD STAPSNENQ
Length:1,579
Mass (Da):177,777
Last modified:October 1, 1993 - v1
Checksum:iC778A0400612ECF4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19T → I in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti38D → E in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti54T → A in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti74G → D in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti94 – 99AMNGSY → SMYESR in AAA18831 (PubMed:8187177).Curated6
Sequence conflicti119 – 126GESISPRE → EKNISSRG in AAA18831 (PubMed:8187177).Curated8
Sequence conflicti152 – 153Missing in AAA18831 (PubMed:8187177).Curated2
Sequence conflicti165 – 167QFE → APV in AAA18831 (PubMed:8187177).Curated3
Sequence conflicti202P → A in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti214S → G in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti283L → I in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti378F → L in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti410S → N in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti426M → V in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti774K → R in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti1089S → F in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti1266G → S in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti1476A → V in AAA18831 (PubMed:8187177).Curated1
Sequence conflicti1557T → A in AAA18831 (PubMed:8187177).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07621 Unassigned DNA. Translation: AAA18831.1.
X68577 Genomic DNA. Translation: CAA48568.1.
Z35778 Genomic DNA. Translation: CAA84836.1.
BK006936 Genomic DNA. Translation: DAA07103.1.
PIRiS25329.
RefSeqiNP_009536.1. NM_001178257.1.

Genome annotation databases

EnsemblFungiiYBL017C; YBL017C; YBL017C.
GeneIDi852264.
KEGGisce:YBL017C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07621 Unassigned DNA. Translation: AAA18831.1.
X68577 Genomic DNA. Translation: CAA48568.1.
Z35778 Genomic DNA. Translation: CAA84836.1.
BK006936 Genomic DNA. Translation: DAA07103.1.
PIRiS25329.
RefSeqiNP_009536.1. NM_001178257.1.

3D structure databases

ProteinModelPortaliP32319.
SMRiP32319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32681. 118 interactors.
DIPiDIP-5900N.
IntActiP32319. 23 interactors.
MINTiMINT-630886.

Protein family/group databases

TCDBi9.A.63.1.1. the retromer-dependnet vacuolar protein sorting (r-vps) family.

Proteomic databases

MaxQBiP32319.
PRIDEiP32319.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL017C; YBL017C; YBL017C.
GeneIDi852264.
KEGGisce:YBL017C.

Organism-specific databases

EuPathDBiFungiDB:YBL017C.
SGDiS000000113. PEP1.

Phylogenomic databases

GeneTreeiENSGT00510000046443.
InParanoidiP32319.
OrthoDBiEOG092C0D5Y.

Enzyme and pathway databases

BioCyciYEAST:G3O-28921-MONOMER.

Miscellaneous databases

PROiP32319.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
InterProiIPR011040. Sialidases.
IPR031777. Sortilin_C.
IPR031778. Sortilin_N.
IPR006581. VPS10.
[Graphical view]
PfamiPF15902. Sortilin-Vps10. 2 hits.
PF15901. Sortilin_C. 2 hits.
[Graphical view]
SMARTiSM00602. VPS10. 2 hits.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiVPS10_YEAST
AccessioniPrimary (citable) accession number: P32319
Secondary accession number(s): D6VPY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7210 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.