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P32319 (VPS10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein sorting/targeting protein VPS10
Alternative name(s):
Carboxypeptidase Y receptor
Short name=CPY receptor
Carboxypeptidase Y-deficient protein 1
Sortilin VPS10
Vacuolar carboxypeptidase sorting receptor VPS10
Vacuolar protein sorting-associated protein 10
Vacuolar protein-targeting protein 1
Gene names
Name:PEP1
Synonyms:VPS10, VPT1
Ordered Locus Names:YBL017C
ORF Names:YBL0302, YBL03.22
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length1579 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein. Prevacuolar compartment membrane; Single-pass type I membrane protein. Note: Cycles between the Golgi apparatus and the prevacuolar compartments. Ref.1 Ref.7 Ref.9

Domain

The lumenal domain contains two regions of approximately 650 AA that exhibit 20% identity. The cytoplasmic domain serves as a Golgi retention/recycling signal. Ref.7 Ref.9 Ref.10

Miscellaneous

Present with 7210 molecules/cell in log phase SD medium. Ref.11

Sequence similarities

Belongs to the VPS10-related sortilin family.

Contains 10 BNR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 15791558Vacuolar protein sorting/targeting protein VPS10
PRO_0000022037

Regions

Topological domain22 – 13971376Lumenal Potential
Transmembrane1398 – 141417Helical; Potential
Topological domain1415 – 1579165Cytoplasmic Potential
Repeat57 – 6812BNR 1
Repeat101 – 11212BNR 2
Repeat179 – 19012BNR 3
Repeat415 – 42612BNR 4
Repeat487 – 49812BNR 5
Repeat533 – 54412BNR 6
Repeat764 – 77512BNR 7
Repeat861 – 87212BNR 8
Repeat1143 – 115412BNR 9
Repeat1184 – 119512BNR 10

Amino acid modifications

Glycosylation961N-linked (GlcNAc...) Potential
Glycosylation1701N-linked (GlcNAc...) Potential
Glycosylation4471N-linked (GlcNAc...) Potential
Glycosylation7931N-linked (GlcNAc...) Potential
Glycosylation10101N-linked (GlcNAc...) Potential
Glycosylation13031N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict191T → I in AAA18831. Ref.1
Sequence conflict381D → E in AAA18831. Ref.1
Sequence conflict541T → A in AAA18831. Ref.1
Sequence conflict741G → D in AAA18831. Ref.1
Sequence conflict94 – 996AMNGSY → SMYESR in AAA18831. Ref.1
Sequence conflict119 – 1268GESISPRE → EKNISSRG in AAA18831. Ref.1
Sequence conflict152 – 1532Missing in AAA18831. Ref.1
Sequence conflict165 – 1673QFE → APV in AAA18831. Ref.1
Sequence conflict2021P → A in AAA18831. Ref.1
Sequence conflict2141S → G in AAA18831. Ref.1
Sequence conflict2831L → I in AAA18831. Ref.1
Sequence conflict3781F → L in AAA18831. Ref.1
Sequence conflict4101S → N in AAA18831. Ref.1
Sequence conflict4261M → V in AAA18831. Ref.1
Sequence conflict7741K → R in AAA18831. Ref.1
Sequence conflict10891S → F in AAA18831. Ref.1
Sequence conflict12661G → S in AAA18831. Ref.1
Sequence conflict14761A → V in AAA18831. Ref.1
Sequence conflict15571T → A in AAA18831. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32319 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: C778A0400612ECF4

FASTA1,579177,777
        10         20         30         40         50         60 
MILLHFVYSL WALLLIPLTN AEEFTPKVTK TIAQDSFDIL SFDDSNTLIR KQDTSVTISF 

        70         80         90        100        110        120 
DDGETWEKVE GIEGEITWIY IDPFNRHDRA VATAMNGSYL YITNDQGKSW ERITLPDSGE 

       130        140        150        160        170        180 
SISPRECYIE THPLNKNYFL AKCNYCEKTE VNNDNEENSG DEEGQFEIFN ITRCTDKVFA 

       190        200        210        220        230        240 
SNDGGKSFSE IKSSLERNEN SPISISDCGF AKTSKDSDLE SSDTSIICLF QNMQLIMDEF 

       250        260        270        280        290        300 
SSPYTESKLV LTTDWGKSLK EFDQFKDKVV NGYRILKSHM VVLTQGDRYN DMSSMDVWVS 

       310        320        330        340        350        360 
NDLSNFKMAY MPTQLRHSMQ GEIYEDAMGR IILPMSRERS DQEEDKGIVS EILISDSQGL 

       370        380        390        400        410        420 
KFSPIPWTAN EVFGYINFYQ PTYLKGTMIA SLYPLSRRRN RKGKAKGVKS KGVTKISVDN 

       430        440        450        460        470        480 
GLTWTMLKVV DPDNADSFDC DITDFENCSL QNMFYTREGS TPTAGILMTT GIVGDGSVFD 

       490        500        510        520        530        540 
WGDQRTFISR DGGLTWKLAF DFPCLYAVGD YGNVIVAIPY NADEDDDPQS EFYYSLDQGK 

       550        560        570        580        590        600 
TWTEYQLETT IYPNEVMNTT PDGSGAKFIL NGFTLAHMDG TTNFIYAIDF STAFNDKTCE 

       610        620        630        640        650        660 
ENDFEDWNLA EGKCVNGVKY KIRRRKQDAQ CLVKKVFEDL QLFETACDKC TEADYECAFE 

       670        680        690        700        710        720 
FVRDATGKCV PDYNLIVLSD VCDKTKKKTV PVKPLQLVKG DKCKKPMTVK SVDISCEGVP 

       730        740        750        760        770        780 
KKGTNDKEIV VTENKFDFKI QFYQYFDTVT DESLLMINSR GEAYISHDGG QTIKRFDSNG 

       790        800        810        820        830        840 
ETIIEVVFNP YYNSSAYLFG SKGSIFSTHD RGYSFMTAKL PEARQLGMPL DFNAKAQDTF 

       850        860        870        880        890        900 
IYYGGKNCES ILSPECHAVA YLTNDGGETF TEMLDNAIHC EFAGSLFKYP SNEDMVMCQV 

       910        920        930        940        950        960 
KEKSSQTRSL VSSTDFFQDD KNTVFENIIG YLSTGGYIIV AVPHENNELR AYVTIDGTEF 

       970        980        990       1000       1010       1020 
AEAKFPYDED VGKQEAFTIL ESEKGSIFLH LATNLVPGRD FGNLLKSNSN GTSFVTLEHA 

      1030       1040       1050       1060       1070       1080 
VNRNTFGYVD FEKIQGLEGI ILTNIVSNSD KVAENKEDKQ LKTKITFNEG SDWNFLKPPK 

      1090       1100       1110       1120       1130       1140 
RDSEGKKFSC SSKSLDECSL HLHGYTERKD IRDTYSSGSA LGMMFGVGNV GPNLLPYKEC 

      1150       1160       1170       1180       1190       1200 
STFFTTDGGE TWAEVKKTPH QWEYGDHGGI LVLVPENSET DSISYSTDFG KTWKDYKFCA 

      1210       1220       1230       1240       1250       1260 
DKVLVKDITT VPRDSALRFL LFGEAADIGG SSFRTYTIDF RNIFERQCDF DITGKESADY 

      1270       1280       1290       1300       1310       1320 
KYSPLGSKSN CLFGHQTEFL RKTDENCFIG NIPLSEFSRN IKNCSCTRQD FECDYNFYKA 

      1330       1340       1350       1360       1370       1380 
NDGTCKLVKG LSPANAADVC KKEPDLIEYF ESSGYRKIPL STCEGGLKLD APSSPHACPG 

      1390       1400       1410       1420       1430       1440 
KEKEFKEKYS VSAGPFAFIF ISILLIIFFA AWFVYDRGIR RNGGFARFGE IRLGDDGLIE 

      1450       1460       1470       1480       1490       1500 
NNNTDRVVNN IVKSGFYVFS NIGSLLQHTK TNIAHAISKI RGRFGNRTGP SYSSLIHDQF 

      1510       1520       1530       1540       1550       1560 
LDEADDLLAG HDEDANDLSS FMDQGSNFEI EEDDVPTLEE EHTSYTDQPT TTDVPDTLPE 

      1570 
GNEENIDRPD STAPSNENQ

« Hide

References

« Hide 'large scale' references
[1]"The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene."
Marcusson E.G., Horazdovsky B.F., Cereghino J.L., Gharakhanian E., Emr S.D.
Cell 77:579-586(1994) [PubMed: 8187177] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"An 11.4 kb DNA segment on the left arm of yeast chromosome II carries the carboxypeptidase Y sorting gene PEP1, as well as ACH1, FUS3 and a putative ARS."
van Dyck L., Purnelle B., Skala J., Goffeau A.
Yeast 8:769-776(1992) [PubMed: 1441754] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Isolation of yeast mutants defective in protein targeting to the vacuole."
Bankaitis V.A., Johnson L.M., Emr S.D.
Proc. Natl. Acad. Sci. U.S.A. 83:9075-9079(1986) [PubMed: 3538017] [Abstract]
Cited for: FUNCTION.
[6]"Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases."
Robinson J.S., Klionsky D.J., Banta L.M., Emr S.D.
Mol. Cell. Biol. 8:4936-4948(1988) [PubMed: 3062374] [Abstract]
Cited for: FUNCTION.
[7]"The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization."
Cereghino J.L., Marcusson E.G., Emr S.D.
Mol. Biol. Cell 6:1089-1102(1995) [PubMed: 8534908] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN.
[8]"Multiple pathways for vacuolar sorting of yeast proteinase A."
Westphal V., Marcusson E.G., Winther J.R., Emr S.D., van den Hazel H.B.
J. Biol. Chem. 271:11865-11870(1996) [PubMed: 8662642] [Abstract]
Cited for: FUNCTION.
[9]"Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases."
Cooper A.A., Stevens T.H.
J. Cell Biol. 133:529-541(1996) [PubMed: 8636229] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN.
[10]"Ligand recognition and domain structure of Vps10p, a vacuolar protein sorting receptor in Saccharomyces cerevisiae."
Jorgensen M.U., Emr S.D., Winther J.R.
Eur. J. Biochem. 260:461-469(1999) [PubMed: 10095782] [Abstract]
Cited for: FUNCTION, DOMAIN.
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U07621 Unassigned DNA. Translation: AAA18831.1.
X68577 Genomic DNA. Translation: CAA48568.1.
Z35778 Genomic DNA. Translation: CAA84836.1.
BK006936 Genomic DNA. Translation: DAA07103.1.
PIRS25329.
RefSeqNP_009536.1. NM_001178257.1.

3D structure databases

ProteinModelPortalP32319.
SMRP32319. Positions 510-546, 760-818, 833-882, 909-967, 1356-1389.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5900N.
IntActP32319. 32 interactions.
MINTMINT-630886.
STRINGP32319.

Proteomic databases

PeptideAtlasP32319.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL017C; YBL017C; YBL017C.
GeneID852264.
KEGGsce:YBL017C.
NMPDRfig|4932.3.peg.229.

Organism-specific databases

CYGDYBL017c.
SGDS000000113. PEP1.

Phylogenomic databases

eggNOGfuNOG04761.
GeneTreeEFGT00050000004688.
HOGENOMHBG395571.
OMAVDFEKIQ.
OrthoDBEOG46T68P.

Gene expression databases

ArrayExpressP32319.
GenevestigatorP32319.
GermOnlineYBL017C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR002860. BNR_rpt.
IPR011040. Neuraminidase.
IPR006581. VPS10.
[Graphical view]
PfamPF02012. BNR. 10 hits.
[Graphical view]
SMARTSM00602. VPS10. 2 hits.
[Graphical view]
SUPFAMSSF50939. Sialidase. 1 hit.
ProtoNetSearch...

Other

NextBio970862.

Entry information

Entry nameVPS10_YEAST
AccessionPrimary (citable) accession number: P32319
Secondary accession number(s): D6VPY3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: December 14, 2011
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

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