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Protein

Vacuolar protein sorting/targeting protein VPS10

Gene

PEP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal.7 Publications

GO - Molecular functioni

  • nucleotide binding Source: UniProtKB-KW
  • signal sequence binding Source: SGD

GO - Biological processi

  • protein targeting to vacuole Source: SGD
  • vacuolar transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28921-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting/targeting protein VPS10
Alternative name(s):
Carboxypeptidase Y receptor
Short name:
CPY receptor
Carboxypeptidase Y-deficient protein 1
Sortilin VPS10
Vacuolar carboxypeptidase sorting receptor VPS10
Vacuolar protein sorting-associated protein 10
Vacuolar protein-targeting protein 1
Gene namesi
Name:PEP1
Synonyms:VPS10, VPT1
Ordered Locus Names:YBL017C
ORF Names:YBL03.22, YBL0302
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL017C.
SGDiS000000113. PEP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 13971376LumenalSequence analysisAdd
BLAST
Transmembranei1398 – 141417HelicalSequence analysisAdd
BLAST
Topological domaini1415 – 1579165CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • late endosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 15791558Vacuolar protein sorting/targeting protein VPS10PRO_0000022037Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence analysis
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence analysis
Glycosylationi447 – 4471N-linked (GlcNAc...)Sequence analysis
Glycosylationi793 – 7931N-linked (GlcNAc...)Sequence analysis
Glycosylationi1010 – 10101N-linked (GlcNAc...)Sequence analysis
Glycosylationi1303 – 13031N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP32319.

Interactioni

Protein-protein interaction databases

BioGridi32681. 118 interactions.
DIPiDIP-5900N.
IntActiP32319. 23 interactions.
MINTiMINT-630886.

Structurei

3D structure databases

ProteinModelPortaliP32319.
SMRiP32319. Positions 365-704, 901-1366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati57 – 6812BNR 1Add
BLAST
Repeati101 – 11212BNR 2Add
BLAST
Repeati179 – 19012BNR 3Add
BLAST
Repeati415 – 42612BNR 4Add
BLAST
Repeati487 – 49812BNR 5Add
BLAST
Repeati533 – 54412BNR 6Add
BLAST
Repeati764 – 77512BNR 7Add
BLAST
Repeati861 – 87212BNR 8Add
BLAST
Repeati1143 – 115412BNR 9Add
BLAST
Repeati1184 – 119512BNR 10Add
BLAST

Domaini

The lumenal domain contains two regions of approximately 650 AA that exhibit 20% identity. The cytoplasmic domain serves as a Golgi retention/recycling signal.3 Publications

Sequence similaritiesi

Belongs to the VPS10-related sortilin family.Curated
Contains 10 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046443.
InParanoidiP32319.
OMAiFRIFANC.
OrthoDBiEOG7DVDKD.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
InterProiIPR011040. Sialidases.
IPR031777. Sortilin_C.
IPR031778. Sortilin_N.
IPR006581. VPS10.
[Graphical view]
PfamiPF15902. Sortilin-Vps10. 2 hits.
PF15901. Sortilin_C. 2 hits.
[Graphical view]
SMARTiSM00602. VPS10. 2 hits.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILLHFVYSL WALLLIPLTN AEEFTPKVTK TIAQDSFDIL SFDDSNTLIR
60 70 80 90 100
KQDTSVTISF DDGETWEKVE GIEGEITWIY IDPFNRHDRA VATAMNGSYL
110 120 130 140 150
YITNDQGKSW ERITLPDSGE SISPRECYIE THPLNKNYFL AKCNYCEKTE
160 170 180 190 200
VNNDNEENSG DEEGQFEIFN ITRCTDKVFA SNDGGKSFSE IKSSLERNEN
210 220 230 240 250
SPISISDCGF AKTSKDSDLE SSDTSIICLF QNMQLIMDEF SSPYTESKLV
260 270 280 290 300
LTTDWGKSLK EFDQFKDKVV NGYRILKSHM VVLTQGDRYN DMSSMDVWVS
310 320 330 340 350
NDLSNFKMAY MPTQLRHSMQ GEIYEDAMGR IILPMSRERS DQEEDKGIVS
360 370 380 390 400
EILISDSQGL KFSPIPWTAN EVFGYINFYQ PTYLKGTMIA SLYPLSRRRN
410 420 430 440 450
RKGKAKGVKS KGVTKISVDN GLTWTMLKVV DPDNADSFDC DITDFENCSL
460 470 480 490 500
QNMFYTREGS TPTAGILMTT GIVGDGSVFD WGDQRTFISR DGGLTWKLAF
510 520 530 540 550
DFPCLYAVGD YGNVIVAIPY NADEDDDPQS EFYYSLDQGK TWTEYQLETT
560 570 580 590 600
IYPNEVMNTT PDGSGAKFIL NGFTLAHMDG TTNFIYAIDF STAFNDKTCE
610 620 630 640 650
ENDFEDWNLA EGKCVNGVKY KIRRRKQDAQ CLVKKVFEDL QLFETACDKC
660 670 680 690 700
TEADYECAFE FVRDATGKCV PDYNLIVLSD VCDKTKKKTV PVKPLQLVKG
710 720 730 740 750
DKCKKPMTVK SVDISCEGVP KKGTNDKEIV VTENKFDFKI QFYQYFDTVT
760 770 780 790 800
DESLLMINSR GEAYISHDGG QTIKRFDSNG ETIIEVVFNP YYNSSAYLFG
810 820 830 840 850
SKGSIFSTHD RGYSFMTAKL PEARQLGMPL DFNAKAQDTF IYYGGKNCES
860 870 880 890 900
ILSPECHAVA YLTNDGGETF TEMLDNAIHC EFAGSLFKYP SNEDMVMCQV
910 920 930 940 950
KEKSSQTRSL VSSTDFFQDD KNTVFENIIG YLSTGGYIIV AVPHENNELR
960 970 980 990 1000
AYVTIDGTEF AEAKFPYDED VGKQEAFTIL ESEKGSIFLH LATNLVPGRD
1010 1020 1030 1040 1050
FGNLLKSNSN GTSFVTLEHA VNRNTFGYVD FEKIQGLEGI ILTNIVSNSD
1060 1070 1080 1090 1100
KVAENKEDKQ LKTKITFNEG SDWNFLKPPK RDSEGKKFSC SSKSLDECSL
1110 1120 1130 1140 1150
HLHGYTERKD IRDTYSSGSA LGMMFGVGNV GPNLLPYKEC STFFTTDGGE
1160 1170 1180 1190 1200
TWAEVKKTPH QWEYGDHGGI LVLVPENSET DSISYSTDFG KTWKDYKFCA
1210 1220 1230 1240 1250
DKVLVKDITT VPRDSALRFL LFGEAADIGG SSFRTYTIDF RNIFERQCDF
1260 1270 1280 1290 1300
DITGKESADY KYSPLGSKSN CLFGHQTEFL RKTDENCFIG NIPLSEFSRN
1310 1320 1330 1340 1350
IKNCSCTRQD FECDYNFYKA NDGTCKLVKG LSPANAADVC KKEPDLIEYF
1360 1370 1380 1390 1400
ESSGYRKIPL STCEGGLKLD APSSPHACPG KEKEFKEKYS VSAGPFAFIF
1410 1420 1430 1440 1450
ISILLIIFFA AWFVYDRGIR RNGGFARFGE IRLGDDGLIE NNNTDRVVNN
1460 1470 1480 1490 1500
IVKSGFYVFS NIGSLLQHTK TNIAHAISKI RGRFGNRTGP SYSSLIHDQF
1510 1520 1530 1540 1550
LDEADDLLAG HDEDANDLSS FMDQGSNFEI EEDDVPTLEE EHTSYTDQPT
1560 1570
TTDVPDTLPE GNEENIDRPD STAPSNENQ
Length:1,579
Mass (Da):177,777
Last modified:October 1, 1993 - v1
Checksum:iC778A0400612ECF4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191T → I in AAA18831 (PubMed:8187177).Curated
Sequence conflicti38 – 381D → E in AAA18831 (PubMed:8187177).Curated
Sequence conflicti54 – 541T → A in AAA18831 (PubMed:8187177).Curated
Sequence conflicti74 – 741G → D in AAA18831 (PubMed:8187177).Curated
Sequence conflicti94 – 996AMNGSY → SMYESR in AAA18831 (PubMed:8187177).Curated
Sequence conflicti119 – 1268GESISPRE → EKNISSRG in AAA18831 (PubMed:8187177).Curated
Sequence conflicti152 – 1532Missing in AAA18831 (PubMed:8187177).Curated
Sequence conflicti165 – 1673QFE → APV in AAA18831 (PubMed:8187177).Curated
Sequence conflicti202 – 2021P → A in AAA18831 (PubMed:8187177).Curated
Sequence conflicti214 – 2141S → G in AAA18831 (PubMed:8187177).Curated
Sequence conflicti283 – 2831L → I in AAA18831 (PubMed:8187177).Curated
Sequence conflicti378 – 3781F → L in AAA18831 (PubMed:8187177).Curated
Sequence conflicti410 – 4101S → N in AAA18831 (PubMed:8187177).Curated
Sequence conflicti426 – 4261M → V in AAA18831 (PubMed:8187177).Curated
Sequence conflicti774 – 7741K → R in AAA18831 (PubMed:8187177).Curated
Sequence conflicti1089 – 10891S → F in AAA18831 (PubMed:8187177).Curated
Sequence conflicti1266 – 12661G → S in AAA18831 (PubMed:8187177).Curated
Sequence conflicti1476 – 14761A → V in AAA18831 (PubMed:8187177).Curated
Sequence conflicti1557 – 15571T → A in AAA18831 (PubMed:8187177).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07621 Unassigned DNA. Translation: AAA18831.1.
X68577 Genomic DNA. Translation: CAA48568.1.
Z35778 Genomic DNA. Translation: CAA84836.1.
BK006936 Genomic DNA. Translation: DAA07103.1.
PIRiS25329.
RefSeqiNP_009536.1. NM_001178257.1.

Genome annotation databases

EnsemblFungiiYBL017C; YBL017C; YBL017C.
GeneIDi852264.
KEGGisce:YBL017C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07621 Unassigned DNA. Translation: AAA18831.1.
X68577 Genomic DNA. Translation: CAA48568.1.
Z35778 Genomic DNA. Translation: CAA84836.1.
BK006936 Genomic DNA. Translation: DAA07103.1.
PIRiS25329.
RefSeqiNP_009536.1. NM_001178257.1.

3D structure databases

ProteinModelPortaliP32319.
SMRiP32319. Positions 365-704, 901-1366.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32681. 118 interactions.
DIPiDIP-5900N.
IntActiP32319. 23 interactions.
MINTiMINT-630886.

Proteomic databases

MaxQBiP32319.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL017C; YBL017C; YBL017C.
GeneIDi852264.
KEGGisce:YBL017C.

Organism-specific databases

EuPathDBiFungiDB:YBL017C.
SGDiS000000113. PEP1.

Phylogenomic databases

GeneTreeiENSGT00510000046443.
InParanoidiP32319.
OMAiFRIFANC.
OrthoDBiEOG7DVDKD.

Enzyme and pathway databases

BioCyciYEAST:G3O-28921-MONOMER.

Miscellaneous databases

PROiP32319.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
InterProiIPR011040. Sialidases.
IPR031777. Sortilin_C.
IPR031778. Sortilin_N.
IPR006581. VPS10.
[Graphical view]
PfamiPF15902. Sortilin-Vps10. 2 hits.
PF15901. Sortilin_C. 2 hits.
[Graphical view]
SMARTiSM00602. VPS10. 2 hits.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene."
    Marcusson E.G., Horazdovsky B.F., Cereghino J.L., Gharakhanian E., Emr S.D.
    Cell 77:579-586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "An 11.4 kb DNA segment on the left arm of yeast chromosome II carries the carboxypeptidase Y sorting gene PEP1, as well as ACH1, FUS3 and a putative ARS."
    van Dyck L., Purnelle B., Skala J., Goffeau A.
    Yeast 8:769-776(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Isolation of yeast mutants defective in protein targeting to the vacuole."
    Bankaitis V.A., Johnson L.M., Emr S.D.
    Proc. Natl. Acad. Sci. U.S.A. 83:9075-9079(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases."
    Robinson J.S., Klionsky D.J., Banta L.M., Emr S.D.
    Mol. Cell. Biol. 8:4936-4948(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization."
    Cereghino J.L., Marcusson E.G., Emr S.D.
    Mol. Biol. Cell 6:1089-1102(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN.
  8. Cited for: FUNCTION.
  9. "Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases."
    Cooper A.A., Stevens T.H.
    J. Cell Biol. 133:529-541(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN.
  10. "Ligand recognition and domain structure of Vps10p, a vacuolar protein sorting receptor in Saccharomyces cerevisiae."
    Jorgensen M.U., Emr S.D., Winther J.R.
    Eur. J. Biochem. 260:461-469(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN.
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVPS10_YEAST
AccessioniPrimary (citable) accession number: P32319
Secondary accession number(s): D6VPY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 6, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7210 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.