Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thiamine thiazole synthase

Gene

THI4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation7 Publications

Cofactori

Fe cationNote: Binds 1 Fe cation per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76Substrate; via amide nitrogen1
Binding sitei105Substrate; via amide nitrogen1
Binding sitei170Substrate; via amide nitrogen and carbonyl oxygen1
Binding sitei207Substrate1
Binding sitei237Substrate1
Binding sitei291Substrate; via amide nitrogen1

GO - Molecular functioni

  • ferrous iron binding Source: SGD
  • oxidoreductase activity Source: InterPro

GO - Biological processi

  • mitochondrial genome maintenance Source: SGD
  • response to stress Source: InterPro
  • thiamine biosynthetic process Source: SGD
  • thiazole biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Iron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-153.
YEAST:MONOMER3O-153.
BRENDAi2.8.1.10. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:THI4UniRule annotation
Synonyms:ESP35, MOL1
Ordered Locus Names:YGR144W
ORF Names:G6620
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR144W.
SGDiS000003376. THI4.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi97E → A or Q: No activity. 1 Publication1
Mutagenesisi200H → N: Partially active, trapping the enzyme at an advanced intermediate, just before the sulfide transfer reaction. 1 Publication1
Mutagenesisi204C → A: Partially active, trapping the enzyme at an advanced intermediate, just before the sulfide transfer reaction. 1 Publication1
Mutagenesisi205C → S: Partially active, with very weak activity toward NAD. 1 Publication1
Mutagenesisi207D → A: No activity. 1 Publication1
Mutagenesisi237H → A or N: No activity. 1 Publication1
Mutagenesisi238D → A: Partially active, with very weak activity toward NAD. 1 Publication1
Mutagenesisi301R → A or Q: No activity. 1 Publication1
Mutagenesisi304P → A: No activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000340581 – 326Thiamine thiazole synthaseAdd BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2052,3-didehydroalanine (Cys)1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-205 to a reaction intermediate, generating a dehydroalanine residue.

Proteomic databases

PRIDEiP32318.

Expressioni

Inductioni

Repressed by thiamine.2 Publications

Interactioni

Subunit structurei

Homooctamer.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-19215,EBI-19215

Protein-protein interaction databases

BioGridi33392. 13 interactors.
DIPiDIP-1703N.
IntActiP32318. 5 interactors.
MINTiMINT-408091.

Structurei

Secondary structure

1326
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 19Combined sources3
Turni25 – 29Combined sources5
Helixi45 – 62Combined sources18
Beta strandi65 – 71Combined sources7
Helixi75 – 87Combined sources13
Beta strandi93 – 96Combined sources4
Beta strandi98 – 101Combined sources4
Turni104 – 107Combined sources4
Beta strandi116 – 119Combined sources4
Turni120 – 122Combined sources3
Helixi123 – 128Combined sources6
Beta strandi137 – 143Combined sources7
Helixi145 – 157Combined sources13
Beta strandi162 – 165Combined sources4
Beta strandi168 – 178Combined sources11
Beta strandi180 – 183Combined sources4
Beta strandi185 – 193Combined sources9
Helixi194 – 197Combined sources4
Turni198 – 201Combined sources4
Beta strandi209 – 214Combined sources6
Beta strandi220 – 222Combined sources3
Beta strandi230 – 233Combined sources4
Beta strandi239 – 241Combined sources3
Helixi244 – 252Combined sources9
Helixi266 – 276Combined sources11
Beta strandi286 – 288Combined sources3
Helixi291 – 297Combined sources7
Helixi307 – 325Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FPZX-ray1.82A/B1-326[»]
4Y4LX-ray2.00A/B/C/D1-326[»]
ProteinModelPortaliP32318.
SMRiP32318.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32318.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 98Substrate binding2
Regioni301 – 303Substrate binding3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048.
InParanoidiP32318.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiEOG092C3CAV.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_03158. THI4. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR027495. Sti35.
IPR002922. Thi4_fam.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

P32318-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATSTATST SASQLHLNST PVTHCLSDIV KKEDWSDFKF APIRESTVSR
60 70 80 90 100
AMTSRYFKDL DKFAVSDVII VGAGSSGLSA AYVIAKNRPD LKVCIIESSV
110 120 130 140 150
APGGGSWLGG QLFSAMVMRK PAHLFLQELE IPYEDEGDYV VVKHAALFIS
160 170 180 190 200
TVLSKVLQLP NVKLFNATCV EDLVTRPPTE KGEVTVAGVV TNWTLVTQAH
210 220 230 240 250
GTQCCMDPNV IELAGYKNDG TRDLSQKHGV ILSTTGHDGP FGAFCAKRIV
260 270 280 290 300
DIDQNQKLGG MKGLDMNHAE HDVVIHSGAY AGVDNMYFAG MEVAELDGLN
310 320
RMGPTFGAMA LSGVHAAEQI LKHFAA
Length:326
Mass (Da):34,991
Last modified:October 1, 1993 - v1
Checksum:i843790F2CE00BF02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61669 Genomic DNA. Translation: CAA43843.1.
Z72929 Genomic DNA. Translation: CAA97157.1.
Z72930 Genomic DNA. Translation: CAA97159.1.
X85807 Genomic DNA. Translation: CAA59802.1.
BK006941 Genomic DNA. Translation: DAA08235.1.
PIRiS25321.
RefSeqiNP_011660.1. NM_001181273.1.

Genome annotation databases

EnsemblFungiiYGR144W; YGR144W; YGR144W.
GeneIDi853047.
KEGGisce:YGR144W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61669 Genomic DNA. Translation: CAA43843.1.
Z72929 Genomic DNA. Translation: CAA97157.1.
Z72930 Genomic DNA. Translation: CAA97159.1.
X85807 Genomic DNA. Translation: CAA59802.1.
BK006941 Genomic DNA. Translation: DAA08235.1.
PIRiS25321.
RefSeqiNP_011660.1. NM_001181273.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FPZX-ray1.82A/B1-326[»]
4Y4LX-ray2.00A/B/C/D1-326[»]
ProteinModelPortaliP32318.
SMRiP32318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33392. 13 interactors.
DIPiDIP-1703N.
IntActiP32318. 5 interactors.
MINTiMINT-408091.

Proteomic databases

PRIDEiP32318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR144W; YGR144W; YGR144W.
GeneIDi853047.
KEGGisce:YGR144W.

Organism-specific databases

EuPathDBiFungiDB:YGR144W.
SGDiS000003376. THI4.

Phylogenomic databases

HOGENOMiHOG000106048.
InParanoidiP32318.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiEOG092C3CAV.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-153.
YEAST:MONOMER3O-153.
BRENDAi2.8.1.10. 984.

Miscellaneous databases

EvolutionaryTraceiP32318.
PROiP32318.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_03158. THI4. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR027495. Sti35.
IPR002922. Thi4_fam.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_YEAST
AccessioniPrimary (citable) accession number: P32318
Secondary accession number(s): D6VUS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Expressed at high levels in the early stationary phase of batch cultures growing on molasses, an industrial medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.