P32318 (THI4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiazole biosynthetic enzyme, mitochondrial

Gene names

Name:	THI4
Synonyms:	ESP35, MOL1
Ordered Locus Names:	YGR144W
ORF Names:	G6620

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in biosynthesis of the thiamine precursor thiazole. Also seems to have a role in mitochondrial DNA damage tolerance. Ref.6
Subcellular location	Mitochondrion Potential.
Induction	Repressed by thiamine. Ref.5
Miscellaneous	Expressed at high levels in the early stationary phase of batch cultures growing on molasses, an industrial medium.
Sequence similarities	Belongs to the THI4 family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD NAD
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial genome maintenance Inferred from mutant phenotype Ref.6. Source: SGD thiamine biosynthetic process Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD mitochondrion Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-19215,EBI-19215
SRP1	Q02821	1	EBI-19215,EBI-1797

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 326

Thiazole biosynthetic enzyme, mitochondrial

PRO_0000034058

Regions

Nucleotide binding

67 – 97

31

FAD or NAD Potential

Secondary structure

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326

Helix Strand Turn

Details...

Helix

17 – 19

3

Turn

25 – 29

5

Helix

45 – 62

18

Beta strand

65 – 71

7

Helix

75 – 87

13

Beta strand

93 – 96

4

Beta strand

98 – 101

4

Turn

104 – 107

4

Helix

110 – 112

3

Beta strand

116 – 119

4

Turn

120 – 122

3

Helix

123 – 128

6

Beta strand

137 – 143

7

Helix

145 – 157

13

Beta strand

162 – 165

4

Beta strand

168 – 175

8

Beta strand

186 – 193

8

Helix

194 – 197

4

Beta strand

209 – 214

6

Beta strand

218 – 220

3

Beta strand

230 – 233

4

Helix

244 – 253

10

Helix

266 – 276

11

Beta strand

286 – 288

3

Helix

291 – 297

7

Helix

307 – 325

19

Sequences

	Sequence		Length	Mass (Da)
	P32318 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 843790F2CE00BF02	FASTA	326	34,991
	10 20 30 40 50 60 MSATSTATST SASQLHLNST PVTHCLSDIV KKEDWSDFKF APIRESTVSR AMTSRYFKDL 70 80 90 100 110 120 DKFAVSDVII VGAGSSGLSA AYVIAKNRPD LKVCIIESSV APGGGSWLGG QLFSAMVMRK 130 140 150 160 170 180 PAHLFLQELE IPYEDEGDYV VVKHAALFIS TVLSKVLQLP NVKLFNATCV EDLVTRPPTE 190 200 210 220 230 240 KGEVTVAGVV TNWTLVTQAH GTQCCMDPNV IELAGYKNDG TRDLSQKHGV ILSTTGHDGP 250 260 270 280 290 300 FGAFCAKRIV DIDQNQKLGG MKGLDMNHAE HDVVIHSGAY AGVDNMYFAG MEVAELDGLN 310 320 RMGPTFGAMA LSGVHAAEQI LKHFAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"MOL1, a Saccharomyces cerevisiae gene that is highly expressed in early stationary phase during growth on molasses." Praekelt U.M., Meacock P.A. Yeast 8:699-710(1992) [PubMed: 1441749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames." Skala J., Nawrocki A., Goffeau A. Yeast 11:1421-1427(1995) [PubMed: 8585325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 205-326. Strain: ATCC 204508 / S288c.
[5]	"Regulation of THI4 (MOL1), a thiamine-biosynthetic gene of Saccharomyces cerevisiae." Praekelt U.M., Byrne K.L., Meacock P.A. Yeast 10:481-490(1994) [PubMed: 7941734] [Abstract] Cited for: THIAMINE REGULATION.
[6]	"Dual role for the yeast THI4 gene in thiamine biosynthesis and DNA damage tolerance." Machado C.R., Praekelt U.M., de Oliveira R.C., Barbosa A.C., Byrne K.L., Meacock P.A., Menck C.F. J. Mol. Biol. 273:114-121(1997) [PubMed: 9367751] [Abstract] Cited for: FUNCTION IN DNA DAMAGE TOLERANCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X61669 Genomic DNA. Translation: CAA43843.1.
Z72929 Genomic DNA. Translation: CAA97157.1.
Z72930 Genomic DNA. Translation: CAA97159.1.
X85807 Genomic DNA. Translation: CAA59802.1.
BK006941 Genomic DNA. Translation: DAA08235.1.

PIR

S25321.

RefSeq

NP_011660.1. NM_001181273.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FPZ	X-ray	1.82	A/B	1-326	[»]

ProteinModelPortal

P32318.

SMR

P32318. Positions 16-326.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1703N.

IntAct

P32318. 7 interactions.

MINT

MINT-408091.

STRING

P32318.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR144W; YGR144W; YGR144W.

GeneID

853047.

KEGG

sce:YGR144W.

NMPDR

fig|4932.3.peg.2779.

Organism-specific databases

CYGD

YGR144w.

SGD

S000003376. THI4.

Phylogenomic databases

eggNOG

fuNOG05536.

GeneTree

EFGT00050000003737.

HOGENOM

HBG325902.

OMA

GLHVDPL.

OrthoDB

EOG45MRF9.

Gene expression databases

ArrayExpress

P32318.

Genevestigator

P32318.

GermOnline

YGR144W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002922. Thi4.
[Graphical view]

KO

K03146.

PANTHER

PTHR10617:SF54. PTHR10617:SF54. 1 hit.

TIGRFAMs

TIGR00292. TIGR00292. 1 hit.

ProtoNet

Search...

Other

NextBio

972960.

Entry information

Entry name

THI4_YEAST

Accession

Primary (citable) accession number: P32318
Secondary accession number(s): D6VUS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program