P32317 (AFG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein AFG1

Gene names

Name:	AFG1
Synonyms:	AFG
Ordered Locus Names:	YEL052W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Miscellaneous	Present with 3570 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the AFG1 ATPase family.
Sequence caution	The sequence AAA34414.1 differs from that shown. Reason: Frameshift at position 375. The sequence AAB23997.1 differs from that shown. Reason: Frameshift at position 375.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to oxidative stress Inferred from mutant phenotype. Source: SGD misfolded or incompletely synthesized protein catabolic process Inferred from mutant phenotype. Source: SGD protein import into peroxisome matrix Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial inner membrane Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Protein AFG1		PRO_0000064477
Regions
	Nucleotide binding	126 – 133	8	ATP Potential
Experimental info
	Sequence conflict	210	1	T → P in AAA34414. Ref.4
	Sequence conflict	210	1	T → P in AAB23997. Ref.4
	Sequence conflict	373	1	A → R in AAA34414. Ref.4
	Sequence conflict	373	1	A → R in AAB23997. Ref.4
	Sequence conflict	399	1	V → A in AAT93168. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P32317 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 630315D29D5A9924	FASTA	509	58,265
	10 20 30 40 50 60 MIALKPNAVR TFRQVQHCSF RICRYQSTKS NKCLTPLQEY DRLVKLGKLR DDTYQRGIIS 70 80 90 100 110 120 SLGDLYDSLV KYVPPVVKTP NAVDQVGGWL NGLKSVFSRG KPKNIGAYVD VSKIGNSIPR 130 140 150 160 170 180 GVYLYGDVGC GKTMLMDLFY TTIPNHLTKK RIHFHQFMQY VHKRSHEIVR EQNLKELGDA 190 200 210 220 230 240 KGKEIDTVPF LAAEIANNSH VLCFDEFQVT DVADAMILRR LMTALLSDDY GVVLFATSNR 250 260 270 280 290 300 HPDELYINGV QRQSFIPCIE LIKHRTKVIF LNSPTDYRKI PRPVSSVYYF PSDTSIKYAS 310 320 330 340 350 360 KECKTRRETH IKEWYNYFAQ ASHTDDSTDS HTVHKTFYDY PLTIWGREFK VPKCTPPRVA 370 380 390 400 410 420 QFTFKQLCGE PLAAGDYLTL AKNFEAFIVT DIPYLSIYVR DEVRRFITFL DAVYDSGGKL 430 440 450 460 470 480 ATTGAADFSS LFVEPEQILN DFELRPTTKE PDSVDTGMVD EMVEKHGFSK EIAKKSQMFA 490 500 LDEERFAFAR ALSRLSQMSS TDWVTKPTY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"AFG1, a new member of the SEC18-NSF, PAS1, CDC48-VCP, TBP family of ATPases." Lee Y.-J., Wickner R.B. Yeast 8:787-790(1992) [PubMed: 1441755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-481.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18779 Genomic DNA. Translation: AAB64990.1. M94535 Genomic RNA. Translation: AAA34414.1. Frameshift. AY693149 Genomic DNA. Translation: AAT93168.1. S47813 Genomic DNA. Translation: AAB23997.1. Frameshift. BK006939 Genomic DNA. Translation: DAA07602.1.
PIR	S30825.
RefSeq	NP_010862.1. NM_001178867.1.
3D structure databases
ProteinModelPortal	P32317.
SMR	P32317. Positions 117-245.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32317. 1 interaction.
STRING	P32317.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL052W; YEL052W; YEL052W.
GeneID	856658.
KEGG	sce:YEL052W.
NMPDR	fig|4932.3.peg.1912.
Organism-specific databases
CYGD	YEL052w.
SGD	S000000778. AFG1.
Phylogenomic databases
eggNOG	fuNOG07717.
HOGENOM	HBG664131.
OMA	LKERCHI.
OrthoDB	EOG4ZW8KB.
Gene expression databases
ArrayExpress	P32317.
Genevestigator	P32317.
GermOnline	YEL052W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005654. ATPase_AFG1-like. [Graphical view]
KO	K06916.
PANTHER	PTHR12169. AFG1_ATPase. 1 hit.
Pfam	PF03969. AFG1_ATPase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982649.

Entry information

Entry name

AFG1_YEAST

Accession

Primary (citable) accession number: P32317
Secondary accession number(s): D3DLJ8, E9P923

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program