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P32316 (ACH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetyl-CoA hydrolase

EC=3.1.2.1
Alternative name(s):
Acetyl-CoA deacylase
Short name=Acetyl-CoA acylase
Gene names
Name:ACH1
Ordered Locus Names:YBL015W
ORF Names:YBL03.18, YBL0304
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Presumably involved in regulating the intracellular acetyl-CoA pool for fatty acid and cholesterol synthesis and fatty acid oxidation. It may be involved in overall regulation of acetylation during melatonin synthesis.

Catalytic activity

Acetyl-CoA + H2O = CoA + acetate.

Subunit structure

Monomer.

Subcellular location

Cytoplasm Ref.5.

Post-translational modification

Glycosylated; contains mannose.

Miscellaneous

Present with 4890 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the acetyl-CoA hydrolase/transferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 526526Acetyl-CoA hydrolase
PRO_0000215524

Amino acid modifications

Modified residue21Blocked amino end (Thr)
Modified residue3501Phosphoserine Ref.7

Experimental info

Sequence conflict3081F → L in AAA34388. Ref.1
Sequence conflict3201A → S in AAA34388. Ref.1
Sequence conflict363 – 3642LG → FP in AAA34388. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P32316 [UniParc].

Last modified January 4, 2005. Version 2.
Checksum: 7133A291F18AA1E4

FASTA52658,712
        10         20         30         40         50         60 
MTISNLLKQR VRYAPYLKKV KEAHELIPLF KNGQYLGWSG FTGVGTPKAV PEALIDHVEK 

        70         80         90        100        110        120 
NNLQGKLRFN LFVGASAGPE ENRWAEHDMI IKRAPHQVGK PIAKAINQGR IEFFDKHLSM 

       130        140        150        160        170        180 
FPQDLTYGFY TRERKDNKIL DYTIIEATAI KEDGSIVPGP SVGGSPEFIT VSDKVIIEVN 

       190        200        210        220        230        240 
TATPSFEGIH DIDMPVNPPF RKPYPYLKVD DKCGVDSIPV DPEKVVAIVE STMRDQVPPN 

       250        260        270        280        290        300 
TPSDDMSRAI AGHLVEFFRN EVKHGRLPEN LLPLQSGIGN IANAVIEGLA GAQFKHLTVW 

       310        320        330        340        350        360 
TEVLQDSFLD LFENGSLDYA TATSVRLTEK GFDRAFANWE NFKHRLCLRS QVVSNNPEMI 

       370        380        390        400        410        420 
RRLGVIAMNT PVEVDIYAHA NSTNVNGSRM LNGLGGSADF LRNAKLSIMH APSARPTKVD 

       430        440        450        460        470        480 
PTGISTIVPM ASHVDQTEHD LDILVTDQGL ADLRGLSPKE RAREIINKCA HPDYQALLTD 

       490        500        510        520 
YLDRAEHYAK KHNCLHEPHM LKNAFKFHTN LAEKGTMKVD SWEPVD 

« Hide

References

« Hide 'large scale' references
[1]"A glucose-repressible gene encodes acetyl-CoA hydrolase from Saccharomyces cerevisiae."
Lee F.-J.S., Lin L.-W., Smith J.A.
J. Biol. Chem. 265:7413-7418(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, BLOCKED N-TERMINUS.
[2]"An 11.4 kb DNA segment on the left arm of yeast chromosome II carries the carboxypeptidase Y sorting gene PEP1, as well as ACH1, FUS3 and a putative ARS."
van Dyck L., Purnelle B., Skala J., Goffeau A.
Yeast 8:769-776(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 174-181, SUBCELLULAR LOCATION.
Strain: ATCC 201238 / W303-1B.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-350, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31036 mRNA. Translation: AAA34388.1.
X68577 Genomic DNA. Translation: CAA48570.1.
Z35776 Genomic DNA. Translation: CAA84834.1.
BK006936 Genomic DNA. Translation: DAA07105.1.
PIRS28549.
RefSeqNP_009538.1. NM_001178255.1.

3D structure databases

ProteinModelPortalP32316.
SMRP32316. Positions 8-517.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32683. 93 interactions.
DIPDIP-6548N.
IntActP32316. 1 interaction.
MINTMINT-681658.
STRING4932.YBL015W.

Proteomic databases

MaxQBP32316.
PaxDbP32316.
PeptideAtlasP32316.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL015W; YBL015W; YBL015W.
GeneID852266.
KEGGsce:YBL015W.

Organism-specific databases

CYGDYBL015w.
SGDS000000111. ACH1.

Phylogenomic databases

eggNOGCOG0427.
GeneTreeENSGT00390000011259.
HOGENOMHOG000172597.
KOK01067.
OMAPRFFENM.
OrthoDBEOG7TQV8M.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-17664.
YEAST:YBL015W-MONOMER.

Gene expression databases

GenevestigatorP32316.

Family and domain databases

InterProIPR026888. AcetylCoA_hyd_C.
IPR003702. ActCoA_hydro.
[Graphical view]
PfamPF13336. AcetylCoA_hyd_C. 1 hit.
PF02550. AcetylCoA_hydro. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970868.
PROP32316.

Entry information

Entry nameACH1_YEAST
AccessionPrimary (citable) accession number: P32316
Secondary accession number(s): D6VPY5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 4, 2005
Last modified: June 11, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families