P32316 (ACH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA hydrolase
EC=3.1.2.1

Alternative name(s):
Acetyl-CoA deacylase
Short name=Acetyl-CoA acylase

Gene names

Name:	ACH1
Ordered Locus Names:	YBL015W
ORF Names:	YBL03.18, YBL0304

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Presumably involved in regulating the intracellular acetyl-CoA pool for fatty acid and cholesterol synthesis and fatty acid oxidation. It may be involved in overall regulation of acetylation during melatonin synthesis.
Catalytic activity	Acetyl-CoA + H2O = CoA + acetate.
Subunit structure	Monomer.
Subcellular location	Cytoplasm Ref.5.
Post-translational modification	Glycosylated; contains mannose.
Miscellaneous	Present with 4890 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the acetyl-CoA hydrolase/transferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	acetate metabolic process Inferred from mutant phenotype PubMed 8841387. Source: SGD acetyl-CoA metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from direct assay PubMed 11746603. Source: SGD mitochondrion Inferred from direct assay PubMed 12606555PubMed 14576278PubMed 16823961. Source: SGD
Molecular_function	acetate CoA-transferase activity Inferred from direct assay PubMed 19298859. Source: SGD acetyl-CoA hydrolase activity Inferred from direct assay PubMed 12606555. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Acetyl-CoA hydrolase		PRO_0000215524
Amino acid modifications
	Modified residue	2	1	Blocked amino end (Thr)
	Modified residue	350	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	308	1	F → L in AAA34388. Ref.1
	Sequence conflict	320	1	A → S in AAA34388. Ref.1
	Sequence conflict	363 – 364	2	LG → FP in AAA34388. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32316 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 7133A291F18AA1E4	FASTA	526	58,712
	10 20 30 40 50 60 MTISNLLKQR VRYAPYLKKV KEAHELIPLF KNGQYLGWSG FTGVGTPKAV PEALIDHVEK 70 80 90 100 110 120 NNLQGKLRFN LFVGASAGPE ENRWAEHDMI IKRAPHQVGK PIAKAINQGR IEFFDKHLSM 130 140 150 160 170 180 FPQDLTYGFY TRERKDNKIL DYTIIEATAI KEDGSIVPGP SVGGSPEFIT VSDKVIIEVN 190 200 210 220 230 240 TATPSFEGIH DIDMPVNPPF RKPYPYLKVD DKCGVDSIPV DPEKVVAIVE STMRDQVPPN 250 260 270 280 290 300 TPSDDMSRAI AGHLVEFFRN EVKHGRLPEN LLPLQSGIGN IANAVIEGLA GAQFKHLTVW 310 320 330 340 350 360 TEVLQDSFLD LFENGSLDYA TATSVRLTEK GFDRAFANWE NFKHRLCLRS QVVSNNPEMI 370 380 390 400 410 420 RRLGVIAMNT PVEVDIYAHA NSTNVNGSRM LNGLGGSADF LRNAKLSIMH APSARPTKVD 430 440 450 460 470 480 PTGISTIVPM ASHVDQTEHD LDILVTDQGL ADLRGLSPKE RAREIINKCA HPDYQALLTD 490 500 510 520 YLDRAEHYAK KHNCLHEPHM LKNAFKFHTN LAEKGTMKVD SWEPVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A glucose-repressible gene encodes acetyl-CoA hydrolase from Saccharomyces cerevisiae." Lee F.-J.S., Lin L.-W., Smith J.A. J. Biol. Chem. 265:7413-7418(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, BLOCKED N-TERMINUS.
[2]	"An 11.4 kb DNA segment on the left arm of yeast chromosome II carries the carboxypeptidase Y sorting gene PEP1, as well as ACH1, FUS3 and a putative ARS." van Dyck L., Purnelle B., Skala J., Goffeau A. Yeast 8:769-776(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 174-181, SUBCELLULAR LOCATION. Strain: ATCC 201238 / W303-1B.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-350, MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31036 mRNA. Translation: AAA34388.1. X68577 Genomic DNA. Translation: CAA48570.1. Z35776 Genomic DNA. Translation: CAA84834.1. BK006936 Genomic DNA. Translation: DAA07105.1.
PIR	S28549.
RefSeq	NP_009538.1. NM_001178255.1.
3D structure databases
ProteinModelPortal	P32316.
SMR	P32316. Positions 8-517.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6548N.
IntAct	P32316. 5 interactions.
MINT	MINT-681658.
STRING	4932.YBL015W.
Proteomic databases
PaxDb	P32316.
PeptideAtlas	P32316.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL015W; YBL015W; YBL015W.
GeneID	852266.
KEGG	sce:YBL015W.
Organism-specific databases
CYGD	YBL015w.
SGD	S000000111. ACH1.
Phylogenomic databases
eggNOG	COG0427.
GeneTree	ENSGT00390000011259.
HOGENOM	HOG000172597.
KO	K01067.
OMA	EVSNHPE.
OrthoDB	EOG4DBXNW.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-17664.
Gene expression databases
Genevestigator	P32316.
GermOnline	YBL015W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR026888. AcetylCoA_hyd_C. IPR003702. ActCoA_hydro. IPR017821. Succinate_CoA_transferase. [Graphical view]
PANTHER	PTHR21432. PTHR21432. 1 hit. PTHR21432:SF10. PTHR21432:SF10. 1 hit.
Pfam	PF13336. AcetylCoA_hyd_C. 1 hit. PF02550. AcetylCoA_hydro. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970868.

Entry information

Entry name

ACH1_YEAST

Accession

Primary (citable) accession number: P32316
Secondary accession number(s): D6VPY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program