ID   5HT7R_RAT               Reviewed;         448 AA.
AC   P32305; P97936;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 2.
DT   27-MAR-2024, entry version 179.
DE   RecName: Full=5-hydroxytryptamine receptor 7;
DE            Short=5-HT-7;
DE            Short=5-HT7;
DE   AltName: Full=5-HT-X;
DE   AltName: Full=GPRFO;
DE   AltName: Full=Serotonin receptor 7;
GN   Name=Htr7;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
RC   STRAIN=Wistar; TISSUE=Brain;
RX   PubMed=8397408; DOI=10.1073/pnas.90.18.8547;
RA   Martial R., Traiffort E., Leurs R., Tardivel-Lacombe J., Diaz J.,
RA   Arrang J.-M., Schwartz J.-C.;
RT   "Molecular cloning, characterization, and localization of a high-affinity
RT   serotonin receptor (5-HT7) activating cAMP formation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:8547-8551(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RC   STRAIN=Wistar; TISSUE=Brain;
RX   PubMed=8517926; DOI=10.1089/dna.1993.12.401;
RA   Meyerhof W., Obermueller F., Fehr S., Richter D.;
RT   "A novel rat serotonin receptor: primary structure, pharmacology, and
RT   expression pattern in distinct brain regions.";
RL   DNA Cell Biol. 12:401-409(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
RC   STRAIN=Sprague-Dawley; TISSUE=Hypothalamus;
RX   PubMed=8398139; DOI=10.1016/0896-6273(93)90149-l;
RA   Lovenberg T.W., Baron B.M., de Lecea L., Miller J.D., Prosser R.A.,
RA   Rea M.A., Foye P.E., Slone A.L., Siegel B.W., Danielson P.E.,
RA   Sutcliffe G.J., Erlander M.G.;
RT   "A novel adenylyl cyclase-activating serotonin receptor (5-HT7) implicated
RT   in the regulation of mammalian circadian rhythms.";
RL   Neuron 11:449-458(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
RC   STRAIN=Sprague-Dawley;
RX   PubMed=8394362; DOI=10.1016/s0021-9258(17)46830-x;
RA   Shen Y., Monsma F.J. Jr., Metcalf M.A., Jose P.A., Hamblin M.W.,
RA   Sibley D.R.;
RT   "Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin
RT   receptor subtype.";
RL   J. Biol. Chem. 268:18200-18204(1993).
RN   [5]
RP   NUCLEOTIDE SEQUENCE (ISOFORM B).
RX   PubMed=9084407; DOI=10.1046/j.1471-4159.1997.68041372.x;
RA   Heidmann D.E.A., Metcalf M.A., Kohen R., Hamblin M.W.;
RT   "Four 5-hydroxytryptamine7 (5-HT7) receptor isoforms in human and rat
RT   produced by alternative splicing: species differences due to altered
RT   intron-exon organization.";
RL   J. Neurochem. 68:1372-1381(1997).
CC   -!- FUNCTION: This is one of the several different receptors for 5-
CC       hydroxytryptamine (serotonin), a biogenic hormone that functions as a
CC       neurotransmitter, a hormone, and a mitogen. The activity of this
CC       receptor is mediated by G proteins that stimulate adenylate cyclase.
CC       This receptor is implicated in the regulation of mammalian circadian
CC       rhythms.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=A;
CC         IsoId=P32305-1; Sequence=Displayed;
CC       Name=B;
CC         IsoId=P32305-2; Sequence=VSP_001858;
CC   -!- TISSUE SPECIFICITY: Thalamus, hypothalamus, and the hippocampal
CC       rudiments.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA42134.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; L19654; AAA40617.1; -; Genomic_DNA.
DR   EMBL; X69663; CAA49352.1; -; mRNA.
DR   EMBL; L22558; AAA42132.1; -; mRNA.
DR   EMBL; L15228; AAA42134.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U68489; AAB48395.1; -; mRNA.
DR   PIR; A47519; A47519.
DR   RefSeq; NP_075227.1; NM_022938.2. [P32305-2]
DR   RefSeq; XP_006231369.1; XM_006231307.2. [P32305-1]
DR   RefSeq; XP_008772122.1; XM_008773900.1.
DR   RefSeq; XP_008772123.1; XM_008773901.1.
DR   AlphaFoldDB; P32305; -.
DR   SMR; P32305; -.
DR   STRING; 10116.ENSRNOP00000025450; -.
DR   BindingDB; P32305; -.
DR   ChEMBL; CHEMBL3223; -.
DR   DrugCentral; P32305; -.
DR   GuidetoPHARMACOLOGY; 12; -.
DR   GlyCosmos; P32305; 2 sites, No reported glycans.
DR   GlyGen; P32305; 2 sites.
DR   PhosphoSitePlus; P32305; -.
DR   PaxDb; 10116-ENSRNOP00000025450; -.
DR   Ensembl; ENSRNOT00000025450.8; ENSRNOP00000025450.6; ENSRNOG00000018827.10. [P32305-2]
DR   Ensembl; ENSRNOT00000111885.1; ENSRNOP00000082600.1; ENSRNOG00000018827.10. [P32305-1]
DR   Ensembl; ENSRNOT00055052855; ENSRNOP00055043688; ENSRNOG00055030434. [P32305-1]
DR   Ensembl; ENSRNOT00060052273; ENSRNOP00060043494; ENSRNOG00060030044. [P32305-1]
DR   Ensembl; ENSRNOT00065052373; ENSRNOP00065043068; ENSRNOG00065030344. [P32305-1]
DR   GeneID; 65032; -.
DR   KEGG; rno:65032; -.
DR   UCSC; RGD:71034; rat. [P32305-1]
DR   AGR; RGD:71034; -.
DR   CTD; 3363; -.
DR   RGD; 71034; Htr7.
DR   eggNOG; KOG3656; Eukaryota.
DR   GeneTree; ENSGT01010000222287; -.
DR   HOGENOM; CLU_009579_11_6_1; -.
DR   InParanoid; P32305; -.
DR   OMA; LXKTLTT; -.
DR   OrthoDB; 2999405at2759; -.
DR   PhylomeDB; P32305; -.
DR   Reactome; R-RNO-390666; Serotonin receptors.
DR   Reactome; R-RNO-9706019; RHOBTB3 ATPase cycle.
DR   PRO; PR:P32305; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000018827; Expressed in brain and 11 other cell types or tissues.
DR   ExpressionAtlas; P32305; baseline and differential.
DR   GO; GO:0043679; C:axon terminus; IDA:RGD.
DR   GO; GO:0030425; C:dendrite; IDA:RGD.
DR   GO; GO:0043025; C:neuronal cell body; IDA:RGD.
DR   GO; GO:0045211; C:postsynaptic membrane; IDA:SynGO.
DR   GO; GO:0042734; C:presynaptic membrane; IDA:SynGO.
DR   GO; GO:0008021; C:synaptic vesicle; IDA:RGD.
DR   GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IDA:RGD.
DR   GO; GO:0030594; F:neurotransmitter receptor activity; IDA:RGD.
DR   GO; GO:0007192; P:adenylate cyclase-activating serotonin receptor signaling pathway; IDA:RGD.
DR   GO; GO:0048266; P:behavioral response to pain; IMP:RGD.
DR   GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR   GO; GO:0007623; P:circadian rhythm; IEA:InterPro.
DR   GO; GO:0050966; P:detection of mechanical stimulus involved in sensory perception of pain; IDA:RGD.
DR   GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:RGD.
DR   GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central.
DR   GO; GO:0060291; P:long-term synaptic potentiation; IMP:RGD.
DR   GO; GO:0007613; P:memory; IMP:RGD.
DR   GO; GO:0014063; P:negative regulation of serotonin secretion; IMP:RGD.
DR   GO; GO:0010976; P:positive regulation of neuron projection development; IDA:RGD.
DR   GO; GO:0099171; P:presynaptic modulation of chemical synaptic transmission; IDA:SynGO.
DR   GO; GO:0062086; P:regulation of vein smooth muscle contraction; IMP:RGD.
DR   GO; GO:0051412; P:response to corticosterone; IDA:RGD.
DR   GO; GO:0051602; P:response to electrical stimulus; IEP:RGD.
DR   GO; GO:0007210; P:serotonin receptor signaling pathway; IDA:RGD.
DR   GO; GO:0014832; P:urinary bladder smooth muscle contraction; IMP:RGD.
DR   GO; GO:0042310; P:vasoconstriction; IEA:InterPro.
DR   CDD; cd15329; 7tmA_5-HT7; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR001069; 5HT_7_rcpt.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   PANTHER; PTHR24247; 5-HYDROXYTRYPTAMINE RECEPTOR; 1.
DR   PANTHER; PTHR24247:SF221; 5-HYDROXYTRYPTAMINE RECEPTOR 7; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00652; 5HT7RECEPTR.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
DR   Genevisible; P32305; RN.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; Disulfide bond;
KW   G-protein coupled receptor; Glycoprotein; Lipoprotein; Membrane; Palmitate;
KW   Receptor; Reference proteome; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..448
FT                   /note="5-hydroxytryptamine receptor 7"
FT                   /id="PRO_0000068981"
FT   TOPO_DOM        1..84
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        85..111
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        112..121
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        122..147
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        148..159
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        160..181
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        182..201
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        202..225
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        226..240
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        241..263
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        264..331
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        332..355
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        356..367
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        368..390
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        391..448
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   LIPID           404
FT                   /note="S-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        5
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        69
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        158..234
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT   VAR_SEQ         436..448
FT                   /note="Missing (in isoform B)"
FT                   /evidence="ECO:0000303|PubMed:8398139"
FT                   /id="VSP_001858"
FT   CONFLICT        110
FT                   /note="C -> S (in Ref. 4; AAA42134)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        149
FT                   /note="G -> D (in Ref. 2; CAA49352)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        168
FT                   /note="C -> Y (in Ref. 2; CAA49352)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        196
FT                   /note="R -> S (in Ref. 2; CAA49352)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        199
FT                   /note="G -> E (in Ref. 2; CAA49352)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        237
FT                   /note="S -> N (in Ref. 2; CAA49352)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        348
FT                   /note="L -> P (in Ref. 2; CAA49352)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        396
FT                   /note="T -> P (in Ref. 4)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        398
FT                   /note="Y -> S (in Ref. 4)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   448 AA;  49837 MW;  746E69C7763B317B CRC64;
     MMDVNSSGRP DLYGHLRSLI LPEVGRGLQD LSPDGGAHPV VSSWMPHLLS GFLEVTASPA
     PTWDAPPDNV SGCGEQINYG RVEKVVIGSI LTLITLLTIA GNCLVVISVC FVKKLRQPSN
     YLIVSLALAD LSVAVAVMPF VSVTDLIGGK WIFGHFFCNV FIAMDVMCCT ASIMTLCVIS
     IDRYLGITRP LTYPVRQNGK CMAKMILSVW LLSASITLPP LFGWAQNVND DKVCLISQDF
     GYTIYSTAVA FYIPMSVMLF MYYQIYKAAR KSAAKHKFPG FPRVQPESVI SLNGVVKLQK
     EVEECANLSR LLKHERKNIS IFKREQKAAT TLGIIVGAFT VCWLPFFLLS TARPFICGTS
     CSCIPLWVER TCLWLGYANS LINPFIYAFF NRDLRTTYRS LLQCQYRNIN RKLSAAGMHE
     ALKLAERPER SEFVLQNSDH CGKKGHDT
//