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P32305 (5HT7R_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 7

Short name=5-HT-7
Short name=5-HT7
Alternative name(s):
5-HT-X
GPRFO
Serotonin receptor 7
Gene names
Name:Htr7
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. This receptor is implicated in the regulation of mammalian circadian rhythms.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Thalamus, hypothalamus, and the hippocampal rudiments.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence caution

The sequence AAA42134.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger

Traceable author statement Ref.1. Source: RGD

adenylate cyclase-activating serotonin receptor signaling pathway

Inferred from direct assay Ref.4. Source: RGD

behavioral response to pain

Inferred from mutant phenotype PubMed 16098671. Source: RGD

detection of mechanical stimulus involved in sensory perception of pain

Inferred from direct assay PubMed 21843960. Source: RGD

dopaminergic neuron differentiation

Inferred from mutant phenotype PubMed 17443768. Source: RGD

long-term synaptic potentiation

Inferred from mutant phenotype PubMed 22314569. Source: RGD

memory

Inferred from mutant phenotype PubMed 18308404. Source: RGD

micturition

Inferred from mutant phenotype PubMed 12967934. Source: RGD

negative regulation of circadian sleep/wake cycle, REM sleep

Inferred from direct assay PubMed 18466985. Source: RGD

negative regulation of serotonin secretion

Inferred from mutant phenotype PubMed 15260125. Source: RGD

positive regulation of neuron projection development

Inferred from direct assay PubMed 19243449. Source: RGD

response to corticosterone

Inferred from direct assay PubMed 19617650. Source: RGD

response to electrical stimulus

Inferred from expression pattern PubMed 18195451. Source: RGD

serotonin receptor signaling pathway

Inferred from direct assay Ref.4. Source: RGD

urinary bladder smooth muscle contraction

Inferred from mutant phenotype PubMed 20969855. Source: RGD

vasoconstriction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentaxon terminus

Inferred from direct assay PubMed 16082681. Source: RGD

dendrite

Inferred from direct assay PubMed 16082681. Source: RGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuronal cell body

Inferred from direct assay PubMed 16082681. Source: RGD

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

rough endoplasmic reticulum

Inferred from direct assay PubMed 16082681. Source: RGD

synaptic vesicle

Inferred from direct assay PubMed 16082681. Source: RGD

   Molecular_functionneurotransmitter receptor activity

Inferred from direct assay Ref.4. Source: RGD

serotonin receptor activity

Inferred from direct assay Ref.4. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: P32305-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: P32305-2)

The sequence of this isoform differs from the canonical sequence as follows:
     436-448: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4484485-hydroxytryptamine receptor 7
PRO_0000068981

Regions

Topological domain1 – 8484Extracellular By similarity
Transmembrane85 – 11127Helical; Name=1; By similarity
Topological domain112 – 12110Cytoplasmic By similarity
Transmembrane122 – 14726Helical; Name=2; By similarity
Topological domain148 – 15912Extracellular By similarity
Transmembrane160 – 18122Helical; Name=3; By similarity
Topological domain182 – 20120Cytoplasmic By similarity
Transmembrane202 – 22524Helical; Name=4; By similarity
Topological domain226 – 24015Extracellular By similarity
Transmembrane241 – 26323Helical; Name=5; By similarity
Topological domain264 – 33168Cytoplasmic By similarity
Transmembrane332 – 35524Helical; Name=6; By similarity
Topological domain356 – 36712Extracellular By similarity
Transmembrane368 – 39023Helical; Name=7; By similarity
Topological domain391 – 44858Cytoplasmic By similarity

Amino acid modifications

Lipidation4041S-palmitoyl cysteine Potential
Glycosylation51N-linked (GlcNAc...) Potential
Glycosylation691N-linked (GlcNAc...) Potential
Disulfide bond158 ↔ 234 By similarity

Natural variations

Alternative sequence436 – 44813Missing in isoform B.
VSP_001858

Experimental info

Sequence conflict1101C → S in AAA42134. Ref.4
Sequence conflict1491G → D in CAA49352. Ref.2
Sequence conflict1681C → Y in CAA49352. Ref.2
Sequence conflict1961R → S in CAA49352. Ref.2
Sequence conflict1991G → E in CAA49352. Ref.2
Sequence conflict2371S → N in CAA49352. Ref.2
Sequence conflict3481L → P in CAA49352. Ref.2
Sequence conflict3961T → P Ref.4
Sequence conflict3981Y → S Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified February 1, 1994. Version 2.
Checksum: 746E69C7763B317B

FASTA44849,837
        10         20         30         40         50         60 
MMDVNSSGRP DLYGHLRSLI LPEVGRGLQD LSPDGGAHPV VSSWMPHLLS GFLEVTASPA 

        70         80         90        100        110        120 
PTWDAPPDNV SGCGEQINYG RVEKVVIGSI LTLITLLTIA GNCLVVISVC FVKKLRQPSN 

       130        140        150        160        170        180 
YLIVSLALAD LSVAVAVMPF VSVTDLIGGK WIFGHFFCNV FIAMDVMCCT ASIMTLCVIS 

       190        200        210        220        230        240 
IDRYLGITRP LTYPVRQNGK CMAKMILSVW LLSASITLPP LFGWAQNVND DKVCLISQDF 

       250        260        270        280        290        300 
GYTIYSTAVA FYIPMSVMLF MYYQIYKAAR KSAAKHKFPG FPRVQPESVI SLNGVVKLQK 

       310        320        330        340        350        360 
EVEECANLSR LLKHERKNIS IFKREQKAAT TLGIIVGAFT VCWLPFFLLS TARPFICGTS 

       370        380        390        400        410        420 
CSCIPLWVER TCLWLGYANS LINPFIYAFF NRDLRTTYRS LLQCQYRNIN RKLSAAGMHE 

       430        440 
ALKLAERPER SEFVLQNSDH CGKKGHDT 

« Hide

Isoform B [UniParc].

Checksum: F92B6BAF23939468
Show »

FASTA43548,428

References

[1]"Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation."
Martial R., Traiffort E., Leurs R., Tardivel-Lacombe J., Diaz J., Arrang J.-M., Schwartz J.-C.
Proc. Natl. Acad. Sci. U.S.A. 90:8547-8551(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
Strain: Wistar.
Tissue: Brain.
[2]"A novel rat serotonin receptor: primary structure, pharmacology, and expression pattern in distinct brain regions."
Meyerhof W., Obermueller F., Fehr S., Richter D.
DNA Cell Biol. 12:401-409(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
Strain: Wistar.
Tissue: Brain.
[3]"A novel adenylyl cyclase-activating serotonin receptor (5-HT7) implicated in the regulation of mammalian circadian rhythms."
Lovenberg T.W., Baron B.M., de Lecea L., Miller J.D., Prosser R.A., Rea M.A., Foye P.E., Slone A.L., Siegel B.W., Danielson P.E., Sutcliffe G.J., Erlander M.G.
Neuron 11:449-458(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
Strain: Sprague-Dawley.
Tissue: Hypothalamus.
[4]"Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype."
Shen Y., Monsma F.J. Jr., Metcalf M.A., Jose P.A., Hamblin M.W., Sibley D.R.
J. Biol. Chem. 268:18200-18204(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
Strain: Sprague-Dawley.
[5]"Four 5-hydroxytryptamine7 (5-HT7) receptor isoforms in human and rat produced by alternative splicing: species differences due to altered intron-exon organization."
Heidmann D.E.A., Metcalf M.A., Kohen R., Hamblin M.W.
J. Neurochem. 68:1372-1381(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM B).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L19654 Genomic DNA. Translation: AAA40617.1.
X69663 mRNA. Translation: CAA49352.1.
L22558 mRNA. Translation: AAA42132.1.
L15228 Genomic DNA. Translation: AAA42134.1. Different initiation.
U68489 mRNA. Translation: AAB48395.1.
PIRA47519.
RefSeqNP_075227.1. NM_022938.2.
XP_006231369.1. XM_006231307.1.
UniGeneRn.87132.

3D structure databases

ProteinModelPortalP32305.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP32305.
ChEMBLCHEMBL3223.
GuidetoPHARMACOLOGY12.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PaxDbP32305.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000025493; ENSRNOP00000025493; ENSRNOG00000018827. [P32305-2]
GeneID65032.
KEGGrno:65032.
UCSCRGD:71034. rat. [P32305-1]

Organism-specific databases

CTD3363.
RGD71034. Htr7.

Phylogenomic databases

eggNOGNOG249628.
GeneTreeENSGT00750000117429.
HOGENOMHOG000239242.
HOVERGENHBG106962.
KOK04163.
OrthoDBEOG7S7SDT.
PhylomeDBP32305.

Gene expression databases

GenevestigatorP32305.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001069. 5HT_7_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24247:SF3. PTHR24247:SF3. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00652. 5HT7RECEPTR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613824.
PROP32305.

Entry information

Entry name5HT7R_RAT
AccessionPrimary (citable) accession number: P32305
Secondary accession number(s): P97936
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries