Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P32305

- 5HT7R_RAT

UniProt

P32305 - 5HT7R_RAT

Protein

5-hydroxytryptamine receptor 7

Gene

Htr7

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (01 Feb 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. This receptor is implicated in the regulation of mammalian circadian rhythms.

    GO - Molecular functioni

    1. neurotransmitter receptor activity Source: RGD
    2. serotonin receptor activity Source: RGD

    GO - Biological processi

    1. adenylate cyclase-activating serotonin receptor signaling pathway Source: RGD
    2. behavioral response to pain Source: RGD
    3. detection of mechanical stimulus involved in sensory perception of pain Source: RGD
    4. dopaminergic neuron differentiation Source: RGD
    5. G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: RGD
    6. long-term synaptic potentiation Source: RGD
    7. memory Source: RGD
    8. micturition Source: RGD
    9. negative regulation of circadian sleep/wake cycle, REM sleep Source: RGD
    10. negative regulation of serotonin secretion Source: RGD
    11. positive regulation of neuron projection development Source: RGD
    12. response to corticosterone Source: RGD
    13. response to electrical stimulus Source: RGD
    14. serotonin receptor signaling pathway Source: RGD
    15. urinary bladder smooth muscle contraction Source: RGD
    16. vasoconstriction Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-hydroxytryptamine receptor 7
    Short name:
    5-HT-7
    Short name:
    5-HT7
    Alternative name(s):
    5-HT-X
    GPRFO
    Serotonin receptor 7
    Gene namesi
    Name:Htr7
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi71034. Htr7.

    Subcellular locationi

    GO - Cellular componenti

    1. axon terminus Source: RGD
    2. dendrite Source: RGD
    3. integral component of membrane Source: UniProtKB-KW
    4. neuronal cell body Source: RGD
    5. plasma membrane Source: UniProtKB-SubCell
    6. rough endoplasmic reticulum Source: RGD
    7. synaptic vesicle Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4484485-hydroxytryptamine receptor 7PRO_0000068981Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi158 ↔ 234PROSITE-ProRule annotation
    Lipidationi404 – 4041S-palmitoyl cysteineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP32305.

    Expressioni

    Tissue specificityi

    Thalamus, hypothalamus, and the hippocampal rudiments.

    Gene expression databases

    GenevestigatoriP32305.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliP32305.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8484ExtracellularBy similarityAdd
    BLAST
    Topological domaini112 – 12110CytoplasmicBy similarity
    Topological domaini148 – 15912ExtracellularBy similarityAdd
    BLAST
    Topological domaini182 – 20120CytoplasmicBy similarityAdd
    BLAST
    Topological domaini226 – 24015ExtracellularBy similarityAdd
    BLAST
    Topological domaini264 – 33168CytoplasmicBy similarityAdd
    BLAST
    Topological domaini356 – 36712ExtracellularBy similarityAdd
    BLAST
    Topological domaini391 – 44858CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei85 – 11127Helical; Name=1By similarityAdd
    BLAST
    Transmembranei122 – 14726Helical; Name=2By similarityAdd
    BLAST
    Transmembranei160 – 18122Helical; Name=3By similarityAdd
    BLAST
    Transmembranei202 – 22524Helical; Name=4By similarityAdd
    BLAST
    Transmembranei241 – 26323Helical; Name=5By similarityAdd
    BLAST
    Transmembranei332 – 35524Helical; Name=6By similarityAdd
    BLAST
    Transmembranei368 – 39023Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249628.
    GeneTreeiENSGT00750000117429.
    HOGENOMiHOG000239242.
    HOVERGENiHBG106962.
    KOiK04163.
    OrthoDBiEOG7S7SDT.
    PhylomeDBiP32305.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR001069. 5HT_7_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00652. 5HT7RECEPTR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: P32305-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMDVNSSGRP DLYGHLRSLI LPEVGRGLQD LSPDGGAHPV VSSWMPHLLS    50
    GFLEVTASPA PTWDAPPDNV SGCGEQINYG RVEKVVIGSI LTLITLLTIA 100
    GNCLVVISVC FVKKLRQPSN YLIVSLALAD LSVAVAVMPF VSVTDLIGGK 150
    WIFGHFFCNV FIAMDVMCCT ASIMTLCVIS IDRYLGITRP LTYPVRQNGK 200
    CMAKMILSVW LLSASITLPP LFGWAQNVND DKVCLISQDF GYTIYSTAVA 250
    FYIPMSVMLF MYYQIYKAAR KSAAKHKFPG FPRVQPESVI SLNGVVKLQK 300
    EVEECANLSR LLKHERKNIS IFKREQKAAT TLGIIVGAFT VCWLPFFLLS 350
    TARPFICGTS CSCIPLWVER TCLWLGYANS LINPFIYAFF NRDLRTTYRS 400
    LLQCQYRNIN RKLSAAGMHE ALKLAERPER SEFVLQNSDH CGKKGHDT 448
    Length:448
    Mass (Da):49,837
    Last modified:February 1, 1994 - v2
    Checksum:i746E69C7763B317B
    GO
    Isoform B (identifier: P32305-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         436-448: Missing.

    Show »
    Length:435
    Mass (Da):48,428
    Checksum:iF92B6BAF23939468
    GO

    Sequence cautioni

    The sequence AAA42134.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti110 – 1101C → S in AAA42134. (PubMed:8394362)Curated
    Sequence conflicti149 – 1491G → D in CAA49352. (PubMed:8517926)Curated
    Sequence conflicti168 – 1681C → Y in CAA49352. (PubMed:8517926)Curated
    Sequence conflicti196 – 1961R → S in CAA49352. (PubMed:8517926)Curated
    Sequence conflicti199 – 1991G → E in CAA49352. (PubMed:8517926)Curated
    Sequence conflicti237 – 2371S → N in CAA49352. (PubMed:8517926)Curated
    Sequence conflicti348 – 3481L → P in CAA49352. (PubMed:8517926)Curated
    Sequence conflicti396 – 3961T → P(PubMed:8394362)Curated
    Sequence conflicti398 – 3981Y → S(PubMed:8394362)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei436 – 44813Missing in isoform B. 1 PublicationVSP_001858Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19654 Genomic DNA. Translation: AAA40617.1.
    X69663 mRNA. Translation: CAA49352.1.
    L22558 mRNA. Translation: AAA42132.1.
    L15228 Genomic DNA. Translation: AAA42134.1. Different initiation.
    U68489 mRNA. Translation: AAB48395.1.
    PIRiA47519.
    RefSeqiNP_075227.1. NM_022938.2. [P32305-2]
    XP_006231369.1. XM_006231307.1. [P32305-1]
    UniGeneiRn.87132.

    Genome annotation databases

    EnsembliENSRNOT00000025493; ENSRNOP00000025493; ENSRNOG00000018827. [P32305-2]
    GeneIDi65032.
    KEGGirno:65032.
    UCSCiRGD:71034. rat. [P32305-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19654 Genomic DNA. Translation: AAA40617.1 .
    X69663 mRNA. Translation: CAA49352.1 .
    L22558 mRNA. Translation: AAA42132.1 .
    L15228 Genomic DNA. Translation: AAA42134.1 . Different initiation.
    U68489 mRNA. Translation: AAB48395.1 .
    PIRi A47519.
    RefSeqi NP_075227.1. NM_022938.2. [P32305-2 ]
    XP_006231369.1. XM_006231307.1. [P32305-1 ]
    UniGenei Rn.87132.

    3D structure databases

    ProteinModelPortali P32305.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P32305.
    ChEMBLi CHEMBL3223.
    GuidetoPHARMACOLOGYi 12.

    Protein family/group databases

    GPCRDBi Search...

    Proteomic databases

    PaxDbi P32305.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025493 ; ENSRNOP00000025493 ; ENSRNOG00000018827 . [P32305-2 ]
    GeneIDi 65032.
    KEGGi rno:65032.
    UCSCi RGD:71034. rat. [P32305-1 ]

    Organism-specific databases

    CTDi 3363.
    RGDi 71034. Htr7.

    Phylogenomic databases

    eggNOGi NOG249628.
    GeneTreei ENSGT00750000117429.
    HOGENOMi HOG000239242.
    HOVERGENi HBG106962.
    KOi K04163.
    OrthoDBi EOG7S7SDT.
    PhylomeDBi P32305.

    Miscellaneous databases

    NextBioi 613824.
    PROi P32305.

    Gene expression databases

    Genevestigatori P32305.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR001069. 5HT_7_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00652. 5HT7RECEPTR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation."
      Martial R., Traiffort E., Leurs R., Tardivel-Lacombe J., Diaz J., Arrang J.-M., Schwartz J.-C.
      Proc. Natl. Acad. Sci. U.S.A. 90:8547-8551(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
      Strain: Wistar.
      Tissue: Brain.
    2. "A novel rat serotonin receptor: primary structure, pharmacology, and expression pattern in distinct brain regions."
      Meyerhof W., Obermueller F., Fehr S., Richter D.
      DNA Cell Biol. 12:401-409(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
      Strain: Wistar.
      Tissue: Brain.
    3. "A novel adenylyl cyclase-activating serotonin receptor (5-HT7) implicated in the regulation of mammalian circadian rhythms."
      Lovenberg T.W., Baron B.M., de Lecea L., Miller J.D., Prosser R.A., Rea M.A., Foye P.E., Slone A.L., Siegel B.W., Danielson P.E., Sutcliffe G.J., Erlander M.G.
      Neuron 11:449-458(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
      Strain: Sprague-Dawley.
      Tissue: Hypothalamus.
    4. "Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype."
      Shen Y., Monsma F.J. Jr., Metcalf M.A., Jose P.A., Hamblin M.W., Sibley D.R.
      J. Biol. Chem. 268:18200-18204(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
      Strain: Sprague-Dawley.
    5. "Four 5-hydroxytryptamine7 (5-HT7) receptor isoforms in human and rat produced by alternative splicing: species differences due to altered intron-exon organization."
      Heidmann D.E.A., Metcalf M.A., Kohen R., Hamblin M.W.
      J. Neurochem. 68:1372-1381(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM B).

    Entry informationi

    Entry namei5HT7R_RAT
    AccessioniPrimary (citable) accession number: P32305
    Secondary accession number(s): P97936
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3