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Protein

5-hydroxytryptamine receptor 7

Gene

Htr7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. This receptor is implicated in the regulation of mammalian circadian rhythms.

GO - Molecular functioni

  • neurotransmitter receptor activity Source: RGD
  • serotonin receptor activity Source: RGD

GO - Biological processi

  • adenylate cyclase-activating serotonin receptor signaling pathway Source: RGD
  • behavioral response to pain Source: RGD
  • circadian rhythm Source: InterPro
  • detection of mechanical stimulus involved in sensory perception of pain Source: RGD
  • dopaminergic neuron differentiation Source: RGD
  • G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: RGD
  • long-term synaptic potentiation Source: RGD
  • memory Source: RGD
  • micturition Source: RGD
  • negative regulation of circadian sleep/wake cycle, REM sleep Source: RGD
  • negative regulation of serotonin secretion Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • response to corticosterone Source: RGD
  • response to electrical stimulus Source: RGD
  • serotonin receptor signaling pathway Source: RGD
  • urinary bladder smooth muscle contraction Source: RGD
  • vasoconstriction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_287445. G alpha (s) signalling events.
REACT_343696. Serotonin receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 7
Short name:
5-HT-7
Short name:
5-HT7
Alternative name(s):
5-HT-X
GPRFO
Serotonin receptor 7
Gene namesi
Name:Htr7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi71034. Htr7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8484ExtracellularBy similarityAdd
BLAST
Transmembranei85 – 11127Helical; Name=1By similarityAdd
BLAST
Topological domaini112 – 12110CytoplasmicBy similarity
Transmembranei122 – 14726Helical; Name=2By similarityAdd
BLAST
Topological domaini148 – 15912ExtracellularBy similarityAdd
BLAST
Transmembranei160 – 18122Helical; Name=3By similarityAdd
BLAST
Topological domaini182 – 20120CytoplasmicBy similarityAdd
BLAST
Transmembranei202 – 22524Helical; Name=4By similarityAdd
BLAST
Topological domaini226 – 24015ExtracellularBy similarityAdd
BLAST
Transmembranei241 – 26323Helical; Name=5By similarityAdd
BLAST
Topological domaini264 – 33168CytoplasmicBy similarityAdd
BLAST
Transmembranei332 – 35524Helical; Name=6By similarityAdd
BLAST
Topological domaini356 – 36712ExtracellularBy similarityAdd
BLAST
Transmembranei368 – 39023Helical; Name=7By similarityAdd
BLAST
Topological domaini391 – 44858CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • axon terminus Source: RGD
  • dendrite Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • neuronal cell body Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
  • rough endoplasmic reticulum Source: RGD
  • synaptic vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4484485-hydroxytryptamine receptor 7PRO_0000068981Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi158 ↔ 234PROSITE-ProRule annotation
Lipidationi404 – 4041S-palmitoyl cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP32305.

Expressioni

Tissue specificityi

Thalamus, hypothalamus, and the hippocampal rudiments.

Gene expression databases

ExpressionAtlasiP32305. baseline and differential.
GenevisibleiP32305. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025450.

Structurei

3D structure databases

ProteinModelPortaliP32305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP32305.
KOiK04163.
OrthoDBiEOG7S7SDT.
PhylomeDBiP32305.

Family and domain databases

InterProiIPR001069. 5HT_7_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00652. 5HT7RECEPTR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P32305-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMDVNSSGRP DLYGHLRSLI LPEVGRGLQD LSPDGGAHPV VSSWMPHLLS
60 70 80 90 100
GFLEVTASPA PTWDAPPDNV SGCGEQINYG RVEKVVIGSI LTLITLLTIA
110 120 130 140 150
GNCLVVISVC FVKKLRQPSN YLIVSLALAD LSVAVAVMPF VSVTDLIGGK
160 170 180 190 200
WIFGHFFCNV FIAMDVMCCT ASIMTLCVIS IDRYLGITRP LTYPVRQNGK
210 220 230 240 250
CMAKMILSVW LLSASITLPP LFGWAQNVND DKVCLISQDF GYTIYSTAVA
260 270 280 290 300
FYIPMSVMLF MYYQIYKAAR KSAAKHKFPG FPRVQPESVI SLNGVVKLQK
310 320 330 340 350
EVEECANLSR LLKHERKNIS IFKREQKAAT TLGIIVGAFT VCWLPFFLLS
360 370 380 390 400
TARPFICGTS CSCIPLWVER TCLWLGYANS LINPFIYAFF NRDLRTTYRS
410 420 430 440
LLQCQYRNIN RKLSAAGMHE ALKLAERPER SEFVLQNSDH CGKKGHDT
Length:448
Mass (Da):49,837
Last modified:February 1, 1994 - v2
Checksum:i746E69C7763B317B
GO
Isoform B (identifier: P32305-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     436-448: Missing.

Show »
Length:435
Mass (Da):48,428
Checksum:iF92B6BAF23939468
GO

Sequence cautioni

The sequence AAA42134.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101C → S in AAA42134 (PubMed:8394362).Curated
Sequence conflicti149 – 1491G → D in CAA49352 (PubMed:8517926).Curated
Sequence conflicti168 – 1681C → Y in CAA49352 (PubMed:8517926).Curated
Sequence conflicti196 – 1961R → S in CAA49352 (PubMed:8517926).Curated
Sequence conflicti199 – 1991G → E in CAA49352 (PubMed:8517926).Curated
Sequence conflicti237 – 2371S → N in CAA49352 (PubMed:8517926).Curated
Sequence conflicti348 – 3481L → P in CAA49352 (PubMed:8517926).Curated
Sequence conflicti396 – 3961T → P (PubMed:8394362).Curated
Sequence conflicti398 – 3981Y → S (PubMed:8394362).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei436 – 44813Missing in isoform B. 1 PublicationVSP_001858Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19654 Genomic DNA. Translation: AAA40617.1.
X69663 mRNA. Translation: CAA49352.1.
L22558 mRNA. Translation: AAA42132.1.
L15228 Genomic DNA. Translation: AAA42134.1. Different initiation.
U68489 mRNA. Translation: AAB48395.1.
PIRiA47519.
RefSeqiNP_075227.1. NM_022938.2. [P32305-2]
XP_006231369.1. XM_006231307.2. [P32305-1]
XP_008772122.1. XM_008773900.1. [P32305-1]
XP_008772123.1. XM_008773901.1. [P32305-2]
UniGeneiRn.87132.

Genome annotation databases

EnsembliENSRNOT00000025450; ENSRNOP00000025450; ENSRNOG00000018827. [P32305-2]
ENSRNOT00000082732; ENSRNOP00000074238; ENSRNOG00000055705. [P32305-1]
GeneIDi103694905.
65032.
KEGGirno:103694905.
rno:65032.
UCSCiRGD:71034. rat. [P32305-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19654 Genomic DNA. Translation: AAA40617.1.
X69663 mRNA. Translation: CAA49352.1.
L22558 mRNA. Translation: AAA42132.1.
L15228 Genomic DNA. Translation: AAA42134.1. Different initiation.
U68489 mRNA. Translation: AAB48395.1.
PIRiA47519.
RefSeqiNP_075227.1. NM_022938.2. [P32305-2]
XP_006231369.1. XM_006231307.2. [P32305-1]
XP_008772122.1. XM_008773900.1. [P32305-1]
XP_008772123.1. XM_008773901.1. [P32305-2]
UniGeneiRn.87132.

3D structure databases

ProteinModelPortaliP32305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025450.

Chemistry

BindingDBiP32305.
ChEMBLiCHEMBL3223.
GuidetoPHARMACOLOGYi12.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiP32305.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025450; ENSRNOP00000025450; ENSRNOG00000018827. [P32305-2]
ENSRNOT00000082732; ENSRNOP00000074238; ENSRNOG00000055705. [P32305-1]
GeneIDi103694905.
65032.
KEGGirno:103694905.
rno:65032.
UCSCiRGD:71034. rat. [P32305-1]

Organism-specific databases

CTDi3363.
RGDi71034. Htr7.

Phylogenomic databases

eggNOGiNOG249628.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP32305.
KOiK04163.
OrthoDBiEOG7S7SDT.
PhylomeDBiP32305.

Enzyme and pathway databases

ReactomeiREACT_287445. G alpha (s) signalling events.
REACT_343696. Serotonin receptors.

Miscellaneous databases

NextBioi613824.
PROiP32305.

Gene expression databases

ExpressionAtlasiP32305. baseline and differential.
GenevisibleiP32305. RN.

Family and domain databases

InterProiIPR001069. 5HT_7_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00652. 5HT7RECEPTR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation."
    Martial R., Traiffort E., Leurs R., Tardivel-Lacombe J., Diaz J., Arrang J.-M., Schwartz J.-C.
    Proc. Natl. Acad. Sci. U.S.A. 90:8547-8551(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
    Strain: Wistar.
    Tissue: Brain.
  2. "A novel rat serotonin receptor: primary structure, pharmacology, and expression pattern in distinct brain regions."
    Meyerhof W., Obermueller F., Fehr S., Richter D.
    DNA Cell Biol. 12:401-409(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Strain: Wistar.
    Tissue: Brain.
  3. "A novel adenylyl cyclase-activating serotonin receptor (5-HT7) implicated in the regulation of mammalian circadian rhythms."
    Lovenberg T.W., Baron B.M., de Lecea L., Miller J.D., Prosser R.A., Rea M.A., Foye P.E., Slone A.L., Siegel B.W., Danielson P.E., Sutcliffe G.J., Erlander M.G.
    Neuron 11:449-458(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    Strain: Sprague-Dawley.
    Tissue: Hypothalamus.
  4. "Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype."
    Shen Y., Monsma F.J. Jr., Metcalf M.A., Jose P.A., Hamblin M.W., Sibley D.R.
    J. Biol. Chem. 268:18200-18204(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
    Strain: Sprague-Dawley.
  5. "Four 5-hydroxytryptamine7 (5-HT7) receptor isoforms in human and rat produced by alternative splicing: species differences due to altered intron-exon organization."
    Heidmann D.E.A., Metcalf M.A., Kohen R., Hamblin M.W.
    J. Neurochem. 68:1372-1381(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM B).

Entry informationi

Entry namei5HT7R_RAT
AccessioniPrimary (citable) accession number: P32305
Secondary accession number(s): P97936
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1994
Last modified: July 22, 2015
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.