P32305 (5HT7R_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 7
Short name=5-HT-7
Short name=5-HT7

Alternative name(s):
5-HT-X
GPRFO
Serotonin receptor 7

Gene names

Name:

Htr7

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. This receptor is implicated in the regulation of mammalian circadian rhythms.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Thalamus, hypothalamus, and the hippocampal rudiments.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence AAA42134.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-activating serotonin receptor signaling pathway Inferred from direct assay Ref.4. Source: RGD behavioral response to pain Inferred from mutant phenotype PubMed 16098671. Source: RGD detection of mechanical stimulus involved in sensory perception of pain Inferred from direct assay PubMed 21843960. Source: RGD dopaminergic neuron differentiation Inferred from mutant phenotype PubMed 17443768. Source: RGD long-term synaptic potentiation Inferred from mutant phenotype PubMed 22314569. Source: RGD memory Inferred from mutant phenotype PubMed 18308404. Source: RGD micturition Inferred from mutant phenotype PubMed 12967934. Source: RGD negative regulation of circadian sleep/wake cycle, REM sleep Inferred from direct assay PubMed 18466985. Source: RGD negative regulation of serotonin secretion Inferred from mutant phenotype PubMed 15260125. Source: RGD positive regulation of neuron projection development Inferred from direct assay PubMed 19243449. Source: RGD response to corticosterone stimulus Inferred from direct assay PubMed 19617650. Source: RGD response to electrical stimulus Inferred from expression pattern PubMed 18195451. Source: RGD urinary bladder smooth muscle contraction Inferred from mutant phenotype PubMed 20969855. Source: RGD
Cellular_component	axon terminus Inferred from direct assay PubMed 16082681. Source: RGD dendrite Inferred from direct assay PubMed 16082681. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from direct assay PubMed 16082681. Source: RGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell rough endoplasmic reticulum Inferred from direct assay PubMed 16082681. Source: RGD synaptic vesicle Inferred from direct assay PubMed 16082681. Source: RGD
Molecular_function	neurotransmitter receptor activity Inferred from direct assay Ref.4. Source: RGD serotonin receptor activity Inferred from direct assay Ref.4. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P32305-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P32305-2)

The sequence of this isoform differs from the canonical sequence as follows:
     436-448: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	5-hydroxytryptamine receptor 7		PRO_0000068981
Regions
	Topological domain	1 – 84	84	Extracellular By similarity
	Transmembrane	85 – 111	27	Helical; Name=1; By similarity
	Topological domain	112 – 121	10	Cytoplasmic By similarity
	Transmembrane	122 – 147	26	Helical; Name=2; By similarity
	Topological domain	148 – 159	12	Extracellular By similarity
	Transmembrane	160 – 181	22	Helical; Name=3; By similarity
	Topological domain	182 – 201	20	Cytoplasmic By similarity
	Transmembrane	202 – 225	24	Helical; Name=4; By similarity
	Topological domain	226 – 240	15	Extracellular By similarity
	Transmembrane	241 – 263	23	Helical; Name=5; By similarity
	Topological domain	264 – 331	68	Cytoplasmic By similarity
	Transmembrane	332 – 355	24	Helical; Name=6; By similarity
	Topological domain	356 – 367	12	Extracellular By similarity
	Transmembrane	368 – 390	23	Helical; Name=7; By similarity
	Topological domain	391 – 448	58	Cytoplasmic By similarity
Amino acid modifications
	Lipidation	404	1	S-palmitoyl cysteine Potential
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Disulfide bond	158 ↔ 234		By similarity
Natural variations
	Alternative sequence	436 – 448	13	Missing in isoform B.		VSP_001858
Experimental info
	Sequence conflict	110	1	C → S in AAA42134. Ref.4
	Sequence conflict	149	1	G → D in CAA49352. Ref.2
	Sequence conflict	168	1	C → Y in CAA49352. Ref.2
	Sequence conflict	196	1	R → S in CAA49352. Ref.2
	Sequence conflict	199	1	G → E in CAA49352. Ref.2
	Sequence conflict	237	1	S → N in CAA49352. Ref.2
	Sequence conflict	348	1	L → P in CAA49352. Ref.2
	Sequence conflict	396	1	T → P Ref.4
	Sequence conflict	398	1	Y → S Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified February 1, 1994. Version 2. Checksum: 746E69C7763B317B	FASTA	448	49,837
	10 20 30 40 50 60 MMDVNSSGRP DLYGHLRSLI LPEVGRGLQD LSPDGGAHPV VSSWMPHLLS GFLEVTASPA 70 80 90 100 110 120 PTWDAPPDNV SGCGEQINYG RVEKVVIGSI LTLITLLTIA GNCLVVISVC FVKKLRQPSN 130 140 150 160 170 180 YLIVSLALAD LSVAVAVMPF VSVTDLIGGK WIFGHFFCNV FIAMDVMCCT ASIMTLCVIS 190 200 210 220 230 240 IDRYLGITRP LTYPVRQNGK CMAKMILSVW LLSASITLPP LFGWAQNVND DKVCLISQDF 250 260 270 280 290 300 GYTIYSTAVA FYIPMSVMLF MYYQIYKAAR KSAAKHKFPG FPRVQPESVI SLNGVVKLQK 310 320 330 340 350 360 EVEECANLSR LLKHERKNIS IFKREQKAAT TLGIIVGAFT VCWLPFFLLS TARPFICGTS 370 380 390 400 410 420 CSCIPLWVER TCLWLGYANS LINPFIYAFF NRDLRTTYRS LLQCQYRNIN RKLSAAGMHE 430 440 ALKLAERPER SEFVLQNSDH CGKKGHDT
	Isoform B [UniParc]. Checksum: F92B6BAF23939468	FASTA	435	48,428
	10 20 30 40 50 60 MMDVNSSGRP DLYGHLRSLI LPEVGRGLQD LSPDGGAHPV VSSWMPHLLS GFLEVTASPA 70 80 90 100 110 120 PTWDAPPDNV SGCGEQINYG RVEKVVIGSI LTLITLLTIA GNCLVVISVC FVKKLRQPSN 130 140 150 160 170 180 YLIVSLALAD LSVAVAVMPF VSVTDLIGGK WIFGHFFCNV FIAMDVMCCT ASIMTLCVIS 190 200 210 220 230 240 IDRYLGITRP LTYPVRQNGK CMAKMILSVW LLSASITLPP LFGWAQNVND DKVCLISQDF 250 260 270 280 290 300 GYTIYSTAVA FYIPMSVMLF MYYQIYKAAR KSAAKHKFPG FPRVQPESVI SLNGVVKLQK 310 320 330 340 350 360 EVEECANLSR LLKHERKNIS IFKREQKAAT TLGIIVGAFT VCWLPFFLLS TARPFICGTS 370 380 390 400 410 420 CSCIPLWVER TCLWLGYANS LINPFIYAFF NRDLRTTYRS LLQCQYRNIN RKLSAAGMHE 430 ALKLAERPER SEFVL

References

[1]	"Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation." Martial R., Traiffort E., Leurs R., Tardivel-Lacombe J., Diaz J., Arrang J.-M., Schwartz J.-C. Proc. Natl. Acad. Sci. U.S.A. 90:8547-8551(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A). Strain: Wistar. Tissue: Brain.
[2]	"A novel rat serotonin receptor: primary structure, pharmacology, and expression pattern in distinct brain regions." Meyerhof W., Obermueller F., Fehr S., Richter D. DNA Cell Biol. 12:401-409(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Strain: Wistar. Tissue: Brain.
[3]	"A novel adenylyl cyclase-activating serotonin receptor (5-HT7) implicated in the regulation of mammalian circadian rhythms." Lovenberg T.W., Baron B.M., de Lecea L., Miller J.D., Prosser R.A., Rea M.A., Foye P.E., Slone A.L., Siegel B.W., Danielson P.E., Sutcliffe G.J., Erlander M.G. Neuron 11:449-458(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Strain: Sprague-Dawley. Tissue: Hypothalamus.
[4]	"Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype." Shen Y., Monsma F.J. Jr., Metcalf M.A., Jose P.A., Hamblin M.W., Sibley D.R. J. Biol. Chem. 268:18200-18204(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A). Strain: Sprague-Dawley.
[5]	"Four 5-hydroxytryptamine7 (5-HT7) receptor isoforms in human and rat produced by alternative splicing: species differences due to altered intron-exon organization." Heidmann D.E.A., Metcalf M.A., Kohen R., Hamblin M.W. J. Neurochem. 68:1372-1381(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM B).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L19654 Genomic DNA. Translation: AAA40617.1. X69663 mRNA. Translation: CAA49352.1. L22558 mRNA. Translation: AAA42132.1. L15228 Genomic DNA. Translation: AAA42134.1. Different initiation. U68489 mRNA. Translation: AAB48395.1.
IPI	IPI00194546. IPI00215109.
PIR	A47519.
RefSeq	NP_075227.1. NM_022938.2.
UniGene	Rn.87132.
3D structure databases
ProteinModelPortal	P32305.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PaxDb	P32305.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025493; ENSRNOP00000025493; ENSRNOG00000018827.
GeneID	65032.
KEGG	rno:65032.
UCSC	RGD:71034. rat.
Organism-specific databases
CTD	3363.
RGD	71034. Htr7.
Phylogenomic databases
eggNOG	NOG249628.
GeneTree	ENSGT00700000104198.
HOGENOM	HOG000239242.
HOVERGEN	HBG106962.
KO	K04163.
OrthoDB	EOG4F1X37.
Gene expression databases
ArrayExpress	P32305.
Genevestigator	P32305.
GermOnline	ENSRNOG00000018827. Rattus norvegicus.
Family and domain databases
InterPro	IPR001069. 5HT_7_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00652. 5HT7RECEPTR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P32305.
ChEMBL	CHEMBL3223.
NextBio	613824.

Entry information

Entry name

5HT7R_RAT

Accession

Primary (citable) accession number: P32305
Secondary accession number(s): P97936

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program