P32301 (GLP1R_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucagon-like peptide 1 receptor
Short name=GLP-1 receptor
Short name=GLP-1-R
Short name=GLP-1R

Gene names

Name:	Glp1r
Synonyms:	Glpr

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Subunit structure	May form homodimers and heterodimers with GIPR By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Pancreatic islets, stomach, lung, rat insulinoma cell line.
Post-translational modification	N-glycosylation enhances cell surface expression and lengthens receptor half-life by preventing degradation in the ER By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	ADP-ribosylation Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-activating G-protein coupled receptor signaling pathway Inferred from mutant phenotype PubMed 8612565. Source: RGD associative learning Inferred from direct assay PubMed 12925848. Source: RGD cAMP-mediated signaling Inferred from mutant phenotype PubMed 10446905. Source: RGD cellular response to glucagon stimulus Inferred from electronic annotation. Source: GOC feeding behavior Inferred from mutant phenotype PubMed 10933308. Source: RGD insulin secretion Inferred from mutant phenotype PubMed 10362617. Source: UniProtKB memory Inferred from direct assay PubMed 12925848. Source: RGD negative regulation of neuron apoptotic process Inferred from direct assay PubMed 12925848. Source: RGD neuropeptide signaling pathway Traceable author statement PubMed 12093887. Source: RGD positive regulation of blood pressure Inferred from direct assay PubMed 12093887. Source: RGD positive regulation of cell differentiation Inferred from mutant phenotype PubMed 10580413. Source: RGD positive regulation of cell proliferation Inferred from mutant phenotype PubMed 10580413. Source: RGD positive regulation of heart contraction Inferred from direct assay PubMed 12093887. Source: RGD positive regulation of insulin secretion Inferred from direct assay PubMed 12093887. Source: RGD positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 11845326. Source: RGD regulation of calcium ion transport Inferred from mutant phenotype PubMed 10446905. Source: RGD release of sequestered calcium ion into cytosol Inferred from direct assay PubMed 15789279. Source: RGD response to glucose stimulus Inferred from mutant phenotype PubMed 11845326. Source: RGD
Cellular_component	integral to plasma membrane Inferred from direct assay PubMed 16002551. Source: RGD
Molecular_function	G-protein coupled peptide receptor activity Inferred from physical interaction PubMed 8612565. Source: RGD glucagon receptor activity Inferred from electronic annotation. Source: InterPro peptide hormone binding Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 463	442	Glucagon-like peptide 1 receptor		PRO_0000012837
Regions
	Topological domain	22 – 145	124	Extracellular Potential
	Transmembrane	146 – 168	23	Helical; Name=1; Potential
	Topological domain	169 – 176	8	Cytoplasmic Potential
	Transmembrane	177 – 196	20	Helical; Name=2; Potential
	Topological domain	197 – 227	31	Extracellular Potential
	Transmembrane	228 – 252	25	Helical; Name=3; Potential
	Topological domain	253 – 264	12	Cytoplasmic Potential
	Transmembrane	265 – 288	24	Helical; Name=4; Potential
	Topological domain	289 – 303	15	Extracellular Potential
	Transmembrane	304 – 329	26	Helical; Name=5; Potential
	Topological domain	330 – 351	22	Cytoplasmic Potential
	Transmembrane	352 – 372	21	Helical; Name=6; Potential
	Topological domain	373 – 387	15	Extracellular Potential
	Transmembrane	388 – 408	21	Helical; Name=7; Potential
	Topological domain	409 – 463	55	Cytoplasmic Potential
Amino acid modifications
	Modified residue	341	1	ADP-ribosylcysteine By similarity
	Modified residue	348	1	ADP-ribosylarginine By similarity
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Disulfide bond	46 ↔ 71		By similarity
	Disulfide bond	62 ↔ 104		By similarity
	Disulfide bond	85 ↔ 126		By similarity
	Disulfide bond	226 ↔ 296		By similarity
Experimental info
	Sequence conflict	323	1	V → I in AAP31860. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32301 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: ABE2183E8EBE621F	FASTA	463	52,877
	10 20 30 40 50 60 MAVTPSLLRL ALLLLGAVGR AGPRPQGATV SLSETVQKWR EYRHQCQRFL TEAPLLATGL 70 80 90 100 110 120 FCNRTFDDYA CWPDGPPGSF VNVSCPWYLP WASSVLQGHV YRFCTAEGIW LHKDNSSLPW 130 140 150 160 170 180 RDLSECEESK QGERNSPEEQ LLSLYIIYTV GYALSFSALV IASAILVSFR HLHCTRNYIH 190 200 210 220 230 240 LNLFASFILR ALSVFIKDAA LKWMYSTAAQ QHQWDGLLSY QDSLGCRLVF LLMQYCVAAN 250 260 270 280 290 300 YYWLLVEGVY LYTLLAFSVF SEQRIFKLYL SIGWGVPLLF VIPWGIVKYL YEDEGCWTRN 310 320 330 340 350 360 SNMNYWLIIR LPILFAIGVN FLVFIRVICI VIAKLKANLM CKTDIKCRLA KSTLTLIPLL 370 380 390 400 410 420 GTHEVIFAFV MDEHARGTLR FVKLFTELSF TSFQGFMVAV LYCFVNNEVQ MEFRKSWERW 430 440 450 460 RLERLNIQRD SSMKPLKCPT SSVSSGATVG SSVYAATCQN SCS

References

[1]	"Expression cloning of the pancreatic beta cell receptor for the gluco-incretin hormone glucagon-like peptide 1." Thorens B. Proc. Natl. Acad. Sci. U.S.A. 89:8641-8645(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Pancreatic islet.
[2]	"Molecular cloning of a cDNA encoding for the GLP-1 receptor expressed in rat lung." Lankat-Buttgereit B., Goke R., Fehmann H.C., Richter G., Goke B. Exp. Clin. Endocrinol. 102:341-347(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M97797 mRNA. Translation: AAA73377.1. S75952 mRNA. Translation: AAP31860.1.
IPI	IPI00769114.
PIR	A46172.
RefSeq	NP_036860.1. NM_012728.1.
UniGene	Rn.11408.
3D structure databases
ProteinModelPortal	P32301.
SMR	P32301. Positions 28-131.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P32301.
Proteomic databases
PRIDE	P32301.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25051.
KEGG	rno:25051.
Organism-specific databases
CTD	2740.
RGD	2703. Glp1r.
Phylogenomic databases
eggNOG	NOG263329.
HOGENOM	HOG000008250.
HOVERGEN	HBG008318.
InParanoid	P32301.
KO	K04581.
Enzyme and pathway databases
Reactome	REACT_113568. Metabolism.
Gene expression databases
ArrayExpress	P32301.
Genevestigator	P32301.
GermOnline	ENSRNOG00000001152. Rattus norvegicus.
Family and domain databases
InterPro	IPR017981. GPCR_2-like. IPR001879. GPCR_2_extracellular_dom. IPR003290. GPCR_2_GLP1/glucagon_rcpt. IPR003292. GPCR_2_GLP1_rcpt. IPR000832. GPCR_2_secretin-like. IPR017983. GPCR_2_secretin-like_CS. [Graphical view]
PANTHER	PTHR12011:SF20. PTHR12011:SF20. 1 hit.
Pfam	PF00002. 7tm_2. 1 hit. PF02793. HRM. 1 hit. [Graphical view]
PRINTS	PR01353. GLUCAGNFAMLY. PR01355. GLUCAGNLIKER. PR00249. GPCRSECRETIN.
SMART	SM00008. HormR. 1 hit. [Graphical view]
PROSITE	PS00649. G_PROTEIN_RECEP_F2_1. 1 hit. PS00650. G_PROTEIN_RECEP_F2_2. 1 hit. PS50227. G_PROTEIN_RECEP_F2_3. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P32301.
ChEMBL	CHEMBL5862.
NextBio	605244.

Entry information

Entry name

GLP1R_RAT

Accession

Primary (citable) accession number: P32301
Secondary accession number(s): Q64073, Q6LD83

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program